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The map label for this gene is kojP [H]

Identifier: 17232481

GI number: 17232481

Start: 5959869

End: 5962148

Strand: Reverse

Name: kojP [H]

Synonym: all4989

Alternate gene names: 17232481

Gene position: 5962148-5959869 (Counterclockwise)

Preceding gene: 17232483

Following gene: 17232480

Centisome position: 92.96

GC content: 45.13

Gene sequence:

>2280_bases
ATGCTTGACGACGTAGATATATCGGTGGAAAATTCCCTTGAGCCATTGCTAGATTCCCATGAATGGAATGTGATTGAGAC
AGAATTTAACCCTACACAACTACACCATAAAGAAACTGTTTTCACTCTCAGCAATGGTTATCTAGGAACCAGGGGAAGTT
TGGAGGAAGGATATCAAGAAGATTTTCCCGCCACAATGATTCATGGCATTTATGACGATACGCAAATTTCCCACACAGAA
TTGGTAAATTGTCCTCACTGGCTGCCGTTGGTAGTGGAGGTGGCAGGTGAACGATTTAGCATGAATTCGGGGGAAGTTCT
CCATTACGAGCGTCGGTTGGACTTGCGCTTGGGTTTACTTAGTCGTGATGTGCGGTGGCGTTCTCCCCAAGGCCATACCT
TAGACTTACACTTTGAGCGTTTTGCTAGTTTGGCAGATCAACACGTATTGGCGATTCGTTGTCAAATCACATCTGTAGAT
TTTACAGGTGAAATTACCGTGACAGCGCGATTTGATGCGGAACCAAAAACCCAAGGCGTTCCCCATTGGCAGACTTTAAA
CCAAGGAGGCGTAGACCAGATTCTTTGGTTACAAAGTCAAACCCTGCATTCTGGTGTTGGCTTGGGGATGAGTGCCAAGT
TAATCATAGATGGTGATGAGACTGCGGTTGTTTCTGTCAAAAATGCCGGTATTCCAACTTTGGAGACTACTTTTGAATGT
AACCCAGGAAAAACAGTCATTGTGGAAAAAATTGTGACTGTGTTTACTTCCAGAGAAACAGAAACTCCAATTGCCGCCGC
ACTAGAAAGATTAGCTGATGAACCCAGATACACAACCCTATTAGCTGCCCATATTGCCGCTTGGGAGCAAGTTTGGCAAA
ATAGCGACATTATTATTGAAGGCGATCGCCAAGCCCAACTCAGTGTCCGTTACAATCTTTTTCAGTTACTATCCGTAGCA
CCACGCCACGACGATCATGTAAGTATAGCCCCTAAAACTCTGTCTGGCTTTGCCTATAGCGGACACATTTTTTGGGACAC
CGAAATATTTATCCTCCCCTTCCTCACATTCACCCAACCAGATTTAGCGCGAAACTTACTCACCTACCGCTACCACACCT
TACCAGGCGCAAGACGCAAAGCCCAAGAAGCTGGTTATCAAGGAGCAATGTTCGCTTGGGAAAGTGCTACCACAGGTGAT
GAAGTTACCCCTCGGTGGGTTCCTGGTGCTAATGGTGAATTAATTAGAATTTGGTGCGGTGATCTTGAAGTACACATTAC
AGCCGATGTGGCTTATGCAGTTTGGCACTACTGGCAAACTACCAACGATGATGAATGGATGCACGACTATGGTGCAGAAA
TAATTCTCGATACAGCCGTGTTTTGGGAAAGTCGCGTCCAGTGGAACGAACAACGCCACAGCTACGACATTCTCGATGTA
ATTGGCCCCGATGAAAACCACGATCGCGTCAATAATAACGCCTTTACCAACCTCATGGTACAGTGGCACTTACAATCAGC
TTTAGCCCTGTGGGATTGGTTCAAACACGTTTATCCCGAAAAAGCCGCGCAACTAGTAGAACAACTTAACCTAACTACAG
AACGTCTACACTGTTGGGCAGAAATTCAAGAACGTATCTTTGTCAATGAAGATTTAACTACAGGTTTAATCGAACAATTT
GAAGGCTTTTTTCAATTAGAAGATGTCAACCTAGCTGATTACGAACCCCGCACAAAATCTTTGCAAGGTTTGTTAGGGAT
TGAAGCCACTAGTCAAAAGCAAATACTCAAGCAGCCTGATGTGTTGATGCTACTTTACTTGCTACGTCAGGCTTATGACT
ATAACACCCTCAAGGTTAACTGGGACTATTACACCCAACGCACCGACCATACTTATGGTTCTTCACTAGGCCCAGCAATT
CATGCCGTCTTAGCTTGCGATCTTAATAACCCTACAGAAGCCTACACTCATTTTTTAAGAGCTGCGTTAGTAGATTTAGA
AGATGTCAGACTCAACGCCGCCGAGGGAATCCACGCCGCCAGCGCAGGAGGAGTTTGGCAAGCTGTTGTTTTAGGGTTCG
GGGGTGTGAGAATGACTCAATTTGGCCCGATGGCTTGTCCTAATCTCCCGCCAACTTGGAAACGCTTGAAATTTAAGCTG
CAATGGCGCAATGAATGGTACGAGTTTGATTTACAACCAGAAACAGCAATGGCAATGGTGACACAGGAAGCTCAAGTTCC
TGTTTTTGCCACTGTACAAGCTGTAATGAGTAATGGGTAA

Upstream 100 bases:

>100_bases
ACGTATCAACAGTGAAAGCCAGGATTTTGTTATCTATGAATCCTATCGATTATGTTTCATCTTGACAACAGCCATCTGCA
AATTTTAGGTTTTCTAACTC

Downstream 100 bases:

>100_bases
TGGGTAATGGGTAATGGGTAATGGGTAATTAGTTTTTCCCATTACCAATTAGTATGAGGGGTTGACTCTCCATCCAACTA
GAGAGTTCACAATAGGCTAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 759; Mature: 759

Protein sequence:

>759_residues
MLDDVDISVENSLEPLLDSHEWNVIETEFNPTQLHHKETVFTLSNGYLGTRGSLEEGYQEDFPATMIHGIYDDTQISHTE
LVNCPHWLPLVVEVAGERFSMNSGEVLHYERRLDLRLGLLSRDVRWRSPQGHTLDLHFERFASLADQHVLAIRCQITSVD
FTGEITVTARFDAEPKTQGVPHWQTLNQGGVDQILWLQSQTLHSGVGLGMSAKLIIDGDETAVVSVKNAGIPTLETTFEC
NPGKTVIVEKIVTVFTSRETETPIAAALERLADEPRYTTLLAAHIAAWEQVWQNSDIIIEGDRQAQLSVRYNLFQLLSVA
PRHDDHVSIAPKTLSGFAYSGHIFWDTEIFILPFLTFTQPDLARNLLTYRYHTLPGARRKAQEAGYQGAMFAWESATTGD
EVTPRWVPGANGELIRIWCGDLEVHITADVAYAVWHYWQTTNDDEWMHDYGAEIILDTAVFWESRVQWNEQRHSYDILDV
IGPDENHDRVNNNAFTNLMVQWHLQSALALWDWFKHVYPEKAAQLVEQLNLTTERLHCWAEIQERIFVNEDLTTGLIEQF
EGFFQLEDVNLADYEPRTKSLQGLLGIEATSQKQILKQPDVLMLLYLLRQAYDYNTLKVNWDYYTQRTDHTYGSSLGPAI
HAVLACDLNNPTEAYTHFLRAALVDLEDVRLNAAEGIHAASAGGVWQAVVLGFGGVRMTQFGPMACPNLPPTWKRLKFKL
QWRNEWYEFDLQPETAMAMVTQEAQVPVFATVQAVMSNG

Sequences:

>Translated_759_residues
MLDDVDISVENSLEPLLDSHEWNVIETEFNPTQLHHKETVFTLSNGYLGTRGSLEEGYQEDFPATMIHGIYDDTQISHTE
LVNCPHWLPLVVEVAGERFSMNSGEVLHYERRLDLRLGLLSRDVRWRSPQGHTLDLHFERFASLADQHVLAIRCQITSVD
FTGEITVTARFDAEPKTQGVPHWQTLNQGGVDQILWLQSQTLHSGVGLGMSAKLIIDGDETAVVSVKNAGIPTLETTFEC
NPGKTVIVEKIVTVFTSRETETPIAAALERLADEPRYTTLLAAHIAAWEQVWQNSDIIIEGDRQAQLSVRYNLFQLLSVA
PRHDDHVSIAPKTLSGFAYSGHIFWDTEIFILPFLTFTQPDLARNLLTYRYHTLPGARRKAQEAGYQGAMFAWESATTGD
EVTPRWVPGANGELIRIWCGDLEVHITADVAYAVWHYWQTTNDDEWMHDYGAEIILDTAVFWESRVQWNEQRHSYDILDV
IGPDENHDRVNNNAFTNLMVQWHLQSALALWDWFKHVYPEKAAQLVEQLNLTTERLHCWAEIQERIFVNEDLTTGLIEQF
EGFFQLEDVNLADYEPRTKSLQGLLGIEATSQKQILKQPDVLMLLYLLRQAYDYNTLKVNWDYYTQRTDHTYGSSLGPAI
HAVLACDLNNPTEAYTHFLRAALVDLEDVRLNAAEGIHAASAGGVWQAVVLGFGGVRMTQFGPMACPNLPPTWKRLKFKL
QWRNEWYEFDLQPETAMAMVTQEAQVPVFATVQAVMSNG
>Mature_759_residues
MLDDVDISVENSLEPLLDSHEWNVIETEFNPTQLHHKETVFTLSNGYLGTRGSLEEGYQEDFPATMIHGIYDDTQISHTE
LVNCPHWLPLVVEVAGERFSMNSGEVLHYERRLDLRLGLLSRDVRWRSPQGHTLDLHFERFASLADQHVLAIRCQITSVD
FTGEITVTARFDAEPKTQGVPHWQTLNQGGVDQILWLQSQTLHSGVGLGMSAKLIIDGDETAVVSVKNAGIPTLETTFEC
NPGKTVIVEKIVTVFTSRETETPIAAALERLADEPRYTTLLAAHIAAWEQVWQNSDIIIEGDRQAQLSVRYNLFQLLSVA
PRHDDHVSIAPKTLSGFAYSGHIFWDTEIFILPFLTFTQPDLARNLLTYRYHTLPGARRKAQEAGYQGAMFAWESATTGD
EVTPRWVPGANGELIRIWCGDLEVHITADVAYAVWHYWQTTNDDEWMHDYGAEIILDTAVFWESRVQWNEQRHSYDILDV
IGPDENHDRVNNNAFTNLMVQWHLQSALALWDWFKHVYPEKAAQLVEQLNLTTERLHCWAEIQERIFVNEDLTTGLIEQF
EGFFQLEDVNLADYEPRTKSLQGLLGIEATSQKQILKQPDVLMLLYLLRQAYDYNTLKVNWDYYTQRTDHTYGSSLGPAI
HAVLACDLNNPTEAYTHFLRAALVDLEDVRLNAAEGIHAASAGGVWQAVVLGFGGVRMTQFGPMACPNLPPTWKRLKFKL
QWRNEWYEFDLQPETAMAMVTQEAQVPVFATVQAVMSNG

Specific function: Hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and beta-D-glucose-1-phosphoric acid. Can act with alpha-1,2-oligoglucans, such as selaginose, as substrate, but more slowly. Inactive when dissaccharides with linkages

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=444, Percent_Identity=29.954954954955, Blast_Score=159, Evalue=8e-39,
Organism=Escherichia coli, GI1787575, Length=714, Percent_Identity=31.7927170868347, Blast_Score=333, Evalue=3e-92,
Organism=Saccharomyces cerevisiae, GI6325283, Length=521, Percent_Identity=23.4165067178503, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24583760, Length=309, Percent_Identity=30.42071197411, Blast_Score=133, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: =2.4.1.230 [H]

Molecular weight: Translated: 86311; Mature: 86311

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDVDISVENSLEPLLDSHEWNVIETEFNPTQLHHKETVFTLSNGYLGTRGSLEEGYQE
CCCCCEEECCCCCHHHHCCCCCCEEEECCCCHHCCCCEEEEEEECCCCCCCCCHHHCHHH
DFPATMIHGIYDDTQISHTELVNCPHWLPLVVEVAGERFSMNSGEVLHYERRLDLRLGLL
CCCHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHCCCEECCCCCCEEEEEECCCEEECHH
SRDVRWRSPQGHTLDLHFERFASLADQHVLAIRCQITSVDFTGEITVTARFDAEPKTQGV
CCCCEECCCCCCEEEEEHHHHHHHHCCEEEEEEEEEEEEEECEEEEEEEEECCCCCCCCC
PHWQTLNQGGVDQILWLQSQTLHSGVGLGMSAKLIIDGDETAVVSVKNAGIPTLETTFEC
CCHHHCCCCCCCCEEEEEHHHHHCCCCCCCEEEEEEECCCEEEEEEECCCCCCEEEEEEC
NPGKTVIVEKIVTVFTSRETETPIAAALERLADEPRYTTLLAAHIAAWEQVWQNSDIIIE
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEE
GDRQAQLSVRYNLFQLLSVAPRHDDHVSIAPKTLSGFAYSGHIFWDTEIFILPFLTFTQP
CCCCEEEEEHHHHHHHHHCCCCCCCCEEECCCHHCCEEEECEEEECCEEEEEEEECCCCH
DLARNLLTYRYHTLPGARRKAQEAGYQGAMFAWESATTGDEVTPRWVPGANGELIRIWCG
HHHHHHHHHHEECCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEC
DLEVHITADVAYAVWHYWQTTNDDEWMHDYGAEIILDTAVFWESRVQWNEQRHSYDILDV
CEEEEEEEHHHEEHHHHHCCCCCCHHHHHCCCEEEEEHHHHHHHHHHHCHHCCCEEEEEE
IGPDENHDRVNNNAFTNLMVQWHLQSALALWDWFKHVYPEKAAQLVEQLNLTTERLHCWA
CCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHH
EIQERIFVNEDLTTGLIEQFEGFFQLEDVNLADYEPRTKSLQGLLGIEATSQKQILKQPD
HHHHHEEECCCHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCC
VLMLLYLLRQAYDYNTLKVNWDYYTQRTDHTYGSSLGPAIHAVLACDLNNPTEAYTHFLR
HHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHEEEEEEECCCCHHHHHHHHHH
AALVDLEDVRLNAAEGIHAASAGGVWQAVVLGFGGVRMTQFGPMACPNLPPTWKRLKFKL
HHHHCHHHHEEECCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHEEEEE
QWRNEWYEFDLQPETAMAMVTQEAQVPVFATVQAVMSNG
EECCCEEEEECCCCHHHHEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLDDVDISVENSLEPLLDSHEWNVIETEFNPTQLHHKETVFTLSNGYLGTRGSLEEGYQE
CCCCCEEECCCCCHHHHCCCCCCEEEECCCCHHCCCCEEEEEEECCCCCCCCCHHHCHHH
DFPATMIHGIYDDTQISHTELVNCPHWLPLVVEVAGERFSMNSGEVLHYERRLDLRLGLL
CCCHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHCCCEECCCCCCEEEEEECCCEEECHH
SRDVRWRSPQGHTLDLHFERFASLADQHVLAIRCQITSVDFTGEITVTARFDAEPKTQGV
CCCCEECCCCCCEEEEEHHHHHHHHCCEEEEEEEEEEEEEECEEEEEEEEECCCCCCCCC
PHWQTLNQGGVDQILWLQSQTLHSGVGLGMSAKLIIDGDETAVVSVKNAGIPTLETTFEC
CCHHHCCCCCCCCEEEEEHHHHHCCCCCCCEEEEEEECCCEEEEEEECCCCCCEEEEEEC
NPGKTVIVEKIVTVFTSRETETPIAAALERLADEPRYTTLLAAHIAAWEQVWQNSDIIIE
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEE
GDRQAQLSVRYNLFQLLSVAPRHDDHVSIAPKTLSGFAYSGHIFWDTEIFILPFLTFTQP
CCCCEEEEEHHHHHHHHHCCCCCCCCEEECCCHHCCEEEECEEEECCEEEEEEEECCCCH
DLARNLLTYRYHTLPGARRKAQEAGYQGAMFAWESATTGDEVTPRWVPGANGELIRIWCG
HHHHHHHHHHEECCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEC
DLEVHITADVAYAVWHYWQTTNDDEWMHDYGAEIILDTAVFWESRVQWNEQRHSYDILDV
CEEEEEEEHHHEEHHHHHCCCCCCHHHHHCCCEEEEEHHHHHHHHHHHCHHCCCEEEEEE
IGPDENHDRVNNNAFTNLMVQWHLQSALALWDWFKHVYPEKAAQLVEQLNLTTERLHCWA
CCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHH
EIQERIFVNEDLTTGLIEQFEGFFQLEDVNLADYEPRTKSLQGLLGIEATSQKQILKQPD
HHHHHEEECCCHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCC
VLMLLYLLRQAYDYNTLKVNWDYYTQRTDHTYGSSLGPAIHAVLACDLNNPTEAYTHFLR
HHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHEEEEEEECCCCHHHHHHHHHH
AALVDLEDVRLNAAEGIHAASAGGVWQAVVLGFGGVRMTQFGPMACPNLPPTWKRLKFKL
HHHHCHHHHEEECCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHEEEEE
QWRNEWYEFDLQPETAMAMVTQEAQVPVFATVQAVMSNG
EECCCEEEEECCCCHHHHEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA