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The map label for this gene is pleD [H]

Identifier: 17232388

GI number: 17232388

Start: 5835841

End: 5837391

Strand: Reverse

Name: pleD [H]

Synonym: all4896

Alternate gene names: 17232388

Gene position: 5837391-5835841 (Counterclockwise)

Preceding gene: 17232389

Following gene: 17232387

Centisome position: 91.01

GC content: 39.65

Gene sequence:

>1551_bases
ATGCCAGTGTCCTCCCTGCACTTAGGCATACTCAATATTATGGCGTTTATCTCTTTATCTACATTATTGTGCCTGATGCC
AACCGCTTATCAAATTGTAGAGTTGATTGCCAAATTTCAAGGAGATGCCCGCGAGCTTCAGCGCAGCCAGCAGATGCAAC
AGCTATTTTTAGATCAAGGCCCATTTGGAGCTTATATCAAAGATGAGCAATCTAGAGTGCTTTACTACAACAAAGAAATC
CAGTCTAGATTTTTGGTGGGGCCAATTGAATGGCTAGGTAAAACAGATAGTGAATTCTTGCCAGATCCAGAACAAGGGCG
GCTGGTAATGGTAAATGATCAACTCGTTTTGAAAACTCTACGCCCATTGAAGTTGATTGAAGAAGTCAAGACACCAGGTA
ATGAGAAACCATATTACTGGCTATCTTTTAAATTTCCGTTTAGTGACCATGTTACGGGAGAGCGTCGCATCGGAGGCATT
AGCATAGATATTACAGAATCGGTAGAAGCGCAACGAGTGATCGCGGATTTGAGTCGGCAGTTAGAAGAAAAAACACTAAT
ATTAGAAGCAAAAAATCAGCAAGTTACATATTTGTCAGATATGGTGAATATGCTCTATACTTGCGAGTCTGAAGATGAAG
TATATCAAGTGGTATCTTTAACTTTTACTAAGCTTTTTCCTAGCGTGAGTGGTTGTATCAACATAATTGCTAATTCCAAG
AATTATGTTGAGATGAGAACTTTTTGGGGAAGCGAAAAAAAGAGTAAAGAGGTATTTTCACAATCAGAGTGTTGGGCATT
ACGGAGAGGTAAATTAAACCTGTTATCACCTCATAACCCAGGACTGATGTGCAGTCATTTAATACATCCTGTTAATGGTA
CACATTTGTGTGTTCCATTGTTAGGACAAATTGAAGTAGTTGGGTTTTTACACATTCATGCCCTGGAAGAAATCGGCTCA
GAAGAACAACAAATTGCTGAGATAATTGCTAGATATTTAGCTTTTGCGCTCAACAATCTCTTAATGAAACAACGTCTAAC
CCATGACAATTTGCGGGACGGGTTAACTCAACTATTCAACCCTAGCTATATGCAAACCATAACAGATCAGAGGCTAGCAG
AAGCTGAACGGTTGGGACAATTTCTTGGCATTGTTTTTCTGGATATTGATAACTTTAAATCTTACAACTCTCGCTATGGT
CATTTAACTGCCAACATTGCTCTCAAGGCATTAGCAAAACTACTTTTAACATCTATTCGCTCTTTTGACATTCCTTGCAG
ATGGGGTGGTGACGAATTTGTGATTGTGATACCTAATGTTACGATGGAGACACTGATTGAGCGAGTAGAGCAACTAAGAG
CAGATATTGCTCAAATGCAGTTCAAGGATGGTGATCAGATCCTTGAAAGTATCACCGCAACTTTTGGCATAGCTATATCA
GAGCCTGGGATTACTGTTAAGGATTTATTGAGTCGAGCAAATCAGGCAATGCTTGAGGCCAAGCGAACAAAAAAGAATCA
GGTTGTTGCATACAGAAACGCCGATAATTAA

Upstream 100 bases:

>100_bases
AGCAACTGTCCCTTCTAAATTCTGGCTAGCCTTTTTCTTATCAGGCGCATTCGTACTATTTTGTGGATTTCATCACTTTT
TGGCGATTTTTGAGCAGCAT

Downstream 100 bases:

>100_bases
ATCTCTTAAGTTAGCAACTTATATGCAGATAGTTGATAAAAGGTCAAATAAGAAAGCCTGGGCAAGTGCATTAACTGAAC
CAGCAAAAGAATTTCCGCTT

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MPVSSLHLGILNIMAFISLSTLLCLMPTAYQIVELIAKFQGDARELQRSQQMQQLFLDQGPFGAYIKDEQSRVLYYNKEI
QSRFLVGPIEWLGKTDSEFLPDPEQGRLVMVNDQLVLKTLRPLKLIEEVKTPGNEKPYYWLSFKFPFSDHVTGERRIGGI
SIDITESVEAQRVIADLSRQLEEKTLILEAKNQQVTYLSDMVNMLYTCESEDEVYQVVSLTFTKLFPSVSGCINIIANSK
NYVEMRTFWGSEKKSKEVFSQSECWALRRGKLNLLSPHNPGLMCSHLIHPVNGTHLCVPLLGQIEVVGFLHIHALEEIGS
EEQQIAEIIARYLAFALNNLLMKQRLTHDNLRDGLTQLFNPSYMQTITDQRLAEAERLGQFLGIVFLDIDNFKSYNSRYG
HLTANIALKALAKLLLTSIRSFDIPCRWGGDEFVIVIPNVTMETLIERVEQLRADIAQMQFKDGDQILESITATFGIAIS
EPGITVKDLLSRANQAMLEAKRTKKNQVVAYRNADN

Sequences:

>Translated_516_residues
MPVSSLHLGILNIMAFISLSTLLCLMPTAYQIVELIAKFQGDARELQRSQQMQQLFLDQGPFGAYIKDEQSRVLYYNKEI
QSRFLVGPIEWLGKTDSEFLPDPEQGRLVMVNDQLVLKTLRPLKLIEEVKTPGNEKPYYWLSFKFPFSDHVTGERRIGGI
SIDITESVEAQRVIADLSRQLEEKTLILEAKNQQVTYLSDMVNMLYTCESEDEVYQVVSLTFTKLFPSVSGCINIIANSK
NYVEMRTFWGSEKKSKEVFSQSECWALRRGKLNLLSPHNPGLMCSHLIHPVNGTHLCVPLLGQIEVVGFLHIHALEEIGS
EEQQIAEIIARYLAFALNNLLMKQRLTHDNLRDGLTQLFNPSYMQTITDQRLAEAERLGQFLGIVFLDIDNFKSYNSRYG
HLTANIALKALAKLLLTSIRSFDIPCRWGGDEFVIVIPNVTMETLIERVEQLRADIAQMQFKDGDQILESITATFGIAIS
EPGITVKDLLSRANQAMLEAKRTKKNQVVAYRNADN
>Mature_515_residues
PVSSLHLGILNIMAFISLSTLLCLMPTAYQIVELIAKFQGDARELQRSQQMQQLFLDQGPFGAYIKDEQSRVLYYNKEIQ
SRFLVGPIEWLGKTDSEFLPDPEQGRLVMVNDQLVLKTLRPLKLIEEVKTPGNEKPYYWLSFKFPFSDHVTGERRIGGIS
IDITESVEAQRVIADLSRQLEEKTLILEAKNQQVTYLSDMVNMLYTCESEDEVYQVVSLTFTKLFPSVSGCINIIANSKN
YVEMRTFWGSEKKSKEVFSQSECWALRRGKLNLLSPHNPGLMCSHLIHPVNGTHLCVPLLGQIEVVGFLHIHALEEIGSE
EQQIAEIIARYLAFALNNLLMKQRLTHDNLRDGLTQLFNPSYMQTITDQRLAEAERLGQFLGIVFLDIDNFKSYNSRYGH
LTANIALKALAKLLLTSIRSFDIPCRWGGDEFVIVIPNVTMETLIERVEQLRADIAQMQFKDGDQILESITATFGIAISE
PGITVKDLLSRANQAMLEAKRTKKNQVVAYRNADN

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=169, Percent_Identity=28.9940828402367, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=29.4478527607362, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=32.7160493827161, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=25.9036144578313, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87082007, Length=135, Percent_Identity=28.1481481481481, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787541, Length=178, Percent_Identity=28.0898876404494, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787262, Length=188, Percent_Identity=25, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=119, Percent_Identity=31.0924369747899, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 58618; Mature: 58486

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSSLHLGILNIMAFISLSTLLCLMPTAYQIVELIAKFQGDARELQRSQQMQQLFLDQG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
PFGAYIKDEQSRVLYYNKEIQSRFLVGPIEWLGKTDSEFLPDPEQGRLVMVNDQLVLKTL
CCCCEEECCCCEEEEECHHHHHHHEECHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHH
RPLKLIEEVKTPGNEKPYYWLSFKFPFSDHVTGERRIGGISIDITESVEAQRVIADLSRQ
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEECCEEEEECCCHHHHHHHHHHHHH
LEEKTLILEAKNQQVTYLSDMVNMLYTCESEDEVYQVVSLTFTKLFPSVSGCINIIANSK
HHHHEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NYVEMRTFWGSEKKSKEVFSQSECWALRRGKLNLLSPHNPGLMCSHLIHPVNGTHLCVPL
CEEEEEECCCCCHHHHHHHCCHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCCCEEEEEH
LGQIEVVGFLHIHALEEIGSEEQQIAEIIARYLAFALNNLLMKQRLTHDNLRDGLTQLFN
HCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
PSYMQTITDQRLAEAERLGQFLGIVFLDIDNFKSYNSRYGHLTANIALKALAKLLLTSIR
CHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
SFDIPCRWGGDEFVIVIPNVTMETLIERVEQLRADIAQMQFKDGDQILESITATFGIAIS
HCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEEC
EPGITVKDLLSRANQAMLEAKRTKKNQVVAYRNADN
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
>Mature Secondary Structure 
PVSSLHLGILNIMAFISLSTLLCLMPTAYQIVELIAKFQGDARELQRSQQMQQLFLDQG
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
PFGAYIKDEQSRVLYYNKEIQSRFLVGPIEWLGKTDSEFLPDPEQGRLVMVNDQLVLKTL
CCCCEEECCCCEEEEECHHHHHHHEECHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHH
RPLKLIEEVKTPGNEKPYYWLSFKFPFSDHVTGERRIGGISIDITESVEAQRVIADLSRQ
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEECCEEEEECCCHHHHHHHHHHHHH
LEEKTLILEAKNQQVTYLSDMVNMLYTCESEDEVYQVVSLTFTKLFPSVSGCINIIANSK
HHHHEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NYVEMRTFWGSEKKSKEVFSQSECWALRRGKLNLLSPHNPGLMCSHLIHPVNGTHLCVPL
CEEEEEECCCCCHHHHHHHCCHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCCCEEEEEH
LGQIEVVGFLHIHALEEIGSEEQQIAEIIARYLAFALNNLLMKQRLTHDNLRDGLTQLFN
HCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
PSYMQTITDQRLAEAERLGQFLGIVFLDIDNFKSYNSRYGHLTANIALKALAKLLLTSIR
CHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
SFDIPCRWGGDEFVIVIPNVTMETLIERVEQLRADIAQMQFKDGDQILESITATFGIAIS
HCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEEC
EPGITVKDLLSRANQAMLEAKRTKKNQVVAYRNADN
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA