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The map label for this gene is sphS [H]

Identifier: 17231994

GI number: 17231994

Start: 5391625

End: 5392929

Strand: Reverse

Name: sphS [H]

Synonym: all4502

Alternate gene names: 17231994

Gene position: 5392929-5391625 (Counterclockwise)

Preceding gene: 17231995

Following gene: 17231993

Centisome position: 84.08

GC content: 44.14

Gene sequence:

>1305_bases
ATGCTCTTATTGGGATTTTTTCTGGGTTTGGCGGTAGGTATTGGGTTATGGATTTGGCAACAGATTCAACTAAACCGCTA
CCTGACGCGAGTTACCCAACCCCTCCACTCCCATACTTCTAAGCTGAAAGTGGCGTTGCCGTTGCTTCCTCGTCTGCAAC
GAGAAATTGCTGCTCTCAAACAGCATCGACTAGATTTGCAACAGTCGCTGCAAACTTACCAAGACCTACTGGAATTCGCA
CCAGTGGGCTATTTGCAGGTCGATGAAGAAAATCAATTGCTGTGGTGTAATCAGCAAGCCAGAGAAATCTTATATTTACA
AAGATGGCAACCGGGACAGGTGCGTTTGTTGCTAGAATTGGTGCGTTCCTATGAACTTGACCGCTTAATTGAGCAAACAC
GCGATCGCCAAAAACCCCAGACTAAAGAGTGGGTATTTCATCCCTCTTGTGATAACGCCGCAGAAATGCCCACCATCAAA
TCCATGATGTTAAGGGCGTTTAGCTTACCACTACCCAATGAACAAGTGGGAGTTTTTCTGGAAAATCGCCAACCCCTTTT
AGACTTGAATCAAGTTAGGGAAAGGTCTTTTTCTGATTTAGCCCATGAATTACGCACACCCCTAACTTCCATTCGCTTGG
TTGCAGAAACCCTGCAAAATCGTCTGGAACCACCCTTAAATCGTTGGGTTGACCGTTTGATGCAGGAAGTGGACAGACTG
GTTAACTTAGTCCAAAGCTGGCTAGAACTGACTCAAATGGAAGCAAATCCTACCATGCAGTTGCAGATGGAAACGGTAGA
ATTGCGATCGCTCATCACTTCAGTGTGGGAAACCCTAGAACCTCTAGCACAGCGTCAAAATCTGTCTCTCTCCTACTCCG
GCCCAGAACAACTATTGATTAAAGCCGACTCAGCCCGGATTTATCAAGTGTTTCTCAACTTGTTAGATAACAGTATTAAG
TACAGCTCACCCAATACCAGTATTCAAATTCAAGCGGAAATCTTATCCTTTAAGAAGAATCATGAAGCTAATTCTGCTCC
TCCCATCCTCGAAATAAATTTGATTGATGCTGGTAAAGGTTTTGCAGATGAAGACTTACCCCATGTTTTTGAGCGATTTT
ATCGAGGAGATAAAGCCCGGACACACTCAGCTTTGCAGGATACTAATATTGTCGGTAATGGTTTAGGTTTAGCTATTGTC
AGGCAAATTATCTTAGCTCACGGAGGTTCCATCAAAGCCATGAACCATTCAGAAATTGGTGGCGCATGGATACAACTTCA
TATACCTGAAGTAGTGGCAAACTGA

Upstream 100 bases:

>100_bases
TATATTGTGACTGTAAGAGGATTTGGATATCGATTCGGATAAGCTATTGAGATAGTTGTTAGTTTTTATTAATCACCCGC
AACTAACAACTTAAACTCAA

Downstream 100 bases:

>100_bases
CAACGCCAAGACTATAGTTAAAGATATCTTCACGTTTGAGACTACAAGTGTGAAAGCTGTCGTTTATAGTCCCAACCCTG
AAAATCCCCAGTTAGCACGA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MLLLGFFLGLAVGIGLWIWQQIQLNRYLTRVTQPLHSHTSKLKVALPLLPRLQREIAALKQHRLDLQQSLQTYQDLLEFA
PVGYLQVDEENQLLWCNQQAREILYLQRWQPGQVRLLLELVRSYELDRLIEQTRDRQKPQTKEWVFHPSCDNAAEMPTIK
SMMLRAFSLPLPNEQVGVFLENRQPLLDLNQVRERSFSDLAHELRTPLTSIRLVAETLQNRLEPPLNRWVDRLMQEVDRL
VNLVQSWLELTQMEANPTMQLQMETVELRSLITSVWETLEPLAQRQNLSLSYSGPEQLLIKADSARIYQVFLNLLDNSIK
YSSPNTSIQIQAEILSFKKNHEANSAPPILEINLIDAGKGFADEDLPHVFERFYRGDKARTHSALQDTNIVGNGLGLAIV
RQIILAHGGSIKAMNHSEIGGAWIQLHIPEVVAN

Sequences:

>Translated_434_residues
MLLLGFFLGLAVGIGLWIWQQIQLNRYLTRVTQPLHSHTSKLKVALPLLPRLQREIAALKQHRLDLQQSLQTYQDLLEFA
PVGYLQVDEENQLLWCNQQAREILYLQRWQPGQVRLLLELVRSYELDRLIEQTRDRQKPQTKEWVFHPSCDNAAEMPTIK
SMMLRAFSLPLPNEQVGVFLENRQPLLDLNQVRERSFSDLAHELRTPLTSIRLVAETLQNRLEPPLNRWVDRLMQEVDRL
VNLVQSWLELTQMEANPTMQLQMETVELRSLITSVWETLEPLAQRQNLSLSYSGPEQLLIKADSARIYQVFLNLLDNSIK
YSSPNTSIQIQAEILSFKKNHEANSAPPILEINLIDAGKGFADEDLPHVFERFYRGDKARTHSALQDTNIVGNGLGLAIV
RQIILAHGGSIKAMNHSEIGGAWIQLHIPEVVAN
>Mature_434_residues
MLLLGFFLGLAVGIGLWIWQQIQLNRYLTRVTQPLHSHTSKLKVALPLLPRLQREIAALKQHRLDLQQSLQTYQDLLEFA
PVGYLQVDEENQLLWCNQQAREILYLQRWQPGQVRLLLELVRSYELDRLIEQTRDRQKPQTKEWVFHPSCDNAAEMPTIK
SMMLRAFSLPLPNEQVGVFLENRQPLLDLNQVRERSFSDLAHELRTPLTSIRLVAETLQNRLEPPLNRWVDRLMQEVDRL
VNLVQSWLELTQMEANPTMQLQMETVELRSLITSVWETLEPLAQRQNLSLSYSGPEQLLIKADSARIYQVFLNLLDNSIK
YSSPNTSIQIQAEILSFKKNHEANSAPPILEINLIDAGKGFADEDLPHVFERFYRGDKARTHSALQDTNIVGNGLGLAIV
RQIILAHGGSIKAMNHSEIGGAWIQLHIPEVVAN

Specific function: Member of the two-component regulatory system sphR/sphS. Sensory kinase. Is involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to phos

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=244, Percent_Identity=31.5573770491803, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1786600, Length=446, Percent_Identity=25.7847533632287, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786912, Length=248, Percent_Identity=32.258064516129, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786783, Length=222, Percent_Identity=27.027027027027, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790436, Length=264, Percent_Identity=28.4090909090909, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788713, Length=241, Percent_Identity=23.6514522821577, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790861, Length=270, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790346, Length=239, Percent_Identity=28.4518828451883, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789808, Length=233, Percent_Identity=28.3261802575107, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=243, Percent_Identity=24.2798353909465, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788279, Length=214, Percent_Identity=26.6355140186916, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790551, Length=247, Percent_Identity=28.3400809716599, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787894, Length=240, Percent_Identity=29.5833333333333, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49881; Mature: 49881

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLGFFLGLAVGIGLWIWQQIQLNRYLTRVTQPLHSHTSKLKVALPLLPRLQREIAALK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHH
QHRLDLQQSLQTYQDLLEFAPVGYLQVDEENQLLWCNQQAREILYLQRWQPGQVRLLLEL
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHH
VRSYELDRLIEQTRDRQKPQTKEWVFHPSCDNAAEMPTIKSMMLRAFSLPLPNEQVGVFL
HHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEE
ENRQPLLDLNQVRERSFSDLAHELRTPLTSIRLVAETLQNRLEPPLNRWVDRLMQEVDRL
CCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VNLVQSWLELTQMEANPTMQLQMETVELRSLITSVWETLEPLAQRQNLSLSYSGPEQLLI
HHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHEEE
KADSARIYQVFLNLLDNSIKYSSPNTSIQIQAEILSFKKNHEANSAPPILEINLIDAGKG
ECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCCEEEEEEEECCCC
FADEDLPHVFERFYRGDKARTHSALQDTNIVGNGLGLAIVRQIILAHGGSIKAMNHSEIG
CCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCC
GAWIQLHIPEVVAN
CEEEEEECHHHHCC
>Mature Secondary Structure
MLLLGFFLGLAVGIGLWIWQQIQLNRYLTRVTQPLHSHTSKLKVALPLLPRLQREIAALK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHH
QHRLDLQQSLQTYQDLLEFAPVGYLQVDEENQLLWCNQQAREILYLQRWQPGQVRLLLEL
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHH
VRSYELDRLIEQTRDRQKPQTKEWVFHPSCDNAAEMPTIKSMMLRAFSLPLPNEQVGVFL
HHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEE
ENRQPLLDLNQVRERSFSDLAHELRTPLTSIRLVAETLQNRLEPPLNRWVDRLMQEVDRL
CCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VNLVQSWLELTQMEANPTMQLQMETVELRSLITSVWETLEPLAQRQNLSLSYSGPEQLLI
HHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHEEE
KADSARIYQVFLNLLDNSIKYSSPNTSIQIQAEILSFKKNHEANSAPPILEINLIDAGKG
ECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCCEEEEEEEECCCC
FADEDLPHVFERFYRGDKARTHSALQDTNIVGNGLGLAIVRQIILAHGGSIKAMNHSEIG
CCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCC
GAWIQLHIPEVVAN
CEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497200 [H]