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The map label for this gene is phoB [C]

Identifier: 17231995

GI number: 17231995

Start: 5392988

End: 5393749

Strand: Reverse

Name: phoB [C]

Synonym: all4503

Alternate gene names: 17231995

Gene position: 5393749-5392988 (Counterclockwise)

Preceding gene: 17231998

Following gene: 17231994

Centisome position: 84.1

GC content: 45.28

Gene sequence:

>762_bases
GTGATATCAATGTACACCAGTGAATCGATCAAGTATTCGGCTAGAACAGAGATTGGACAAACCAGTCGGATTCTGGTAGT
GGAAGATGAAGAACTCATCCAAGAAATGCTAGCAGTAGCACTGGAAGAGGAAGGTTATGGGGTAGTAACGGCTGCTGATG
GCAGGTCAGCTGTAGAGTATCTCAAAAGTTTTGACCCAAACGGAGGAGATTTTCCCTTTGATTTGGTGATTCTCGATTTG
ATGTTACCCCAAATTAATGGCTTAGATATCTGCCGTTTGTTGCGTCATCAAGGCAACCCAGTACCGATTTTGATGCTCAG
TGCCAAAGGTAGTGAAACCGATCGCGTGTTAGGTTTAGAAGTAGGGGCAGATGACTATTTAACTAAACCCTTTAGTATGC
GGGAGTTGGTGGCACGTTGTCGCGCTTTACTCCGTCGTCAGCGTCTCATCACTTTACCCCAATTACCTGTACTGAAATTC
AAAGATGTGACTTTAAATCCTCAAGAGTGTCGGGTCTTGGTACGAGGACAAGAGGTAAATCTTTCACCCAAAGAGTTTCG
CTTGTTGGAACTGTTCATGAGTTACGCCCGTCGAGTATGGTCACGGGAACAGTTGCTAGACCAGGTTTGGGGGCCGGATT
TTGTCGGCGATAGCAAAACAGTGGATGTTCACATTCGTTGGTTGCGAGAGAAATTGGAACAAGACCCCAGTCACCCAGAA
TATATTGTGACTGTAAGAGGATTTGGATATCGATTCGGATAA

Upstream 100 bases:

>100_bases
ACTTGCAGAATCATTCACGATCGCCAAAGTGTGAATGTAGTGAAAAACAGATAATCAACTCTAACCACCATATTGATACA
ACCACAGGGCCTGAACTTTT

Downstream 100 bases:

>100_bases
GCTATTGAGATAGTTGTTAGTTTTTATTAATCACCCGCAACTAACAACTTAAACTCAAATGCTCTTATTGGGATTTTTTC
TGGGTTTGGCGGTAGGTATT

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MISMYTSESIKYSARTEIGQTSRILVVEDEELIQEMLAVALEEEGYGVVTAADGRSAVEYLKSFDPNGGDFPFDLVILDL
MLPQINGLDICRLLRHQGNPVPILMLSAKGSETDRVLGLEVGADDYLTKPFSMRELVARCRALLRRQRLITLPQLPVLKF
KDVTLNPQECRVLVRGQEVNLSPKEFRLLELFMSYARRVWSREQLLDQVWGPDFVGDSKTVDVHIRWLREKLEQDPSHPE
YIVTVRGFGYRFG

Sequences:

>Translated_253_residues
MISMYTSESIKYSARTEIGQTSRILVVEDEELIQEMLAVALEEEGYGVVTAADGRSAVEYLKSFDPNGGDFPFDLVILDL
MLPQINGLDICRLLRHQGNPVPILMLSAKGSETDRVLGLEVGADDYLTKPFSMRELVARCRALLRRQRLITLPQLPVLKF
KDVTLNPQECRVLVRGQEVNLSPKEFRLLELFMSYARRVWSREQLLDQVWGPDFVGDSKTVDVHIRWLREKLEQDPSHPE
YIVTVRGFGYRFG
>Mature_253_residues
MISMYTSESIKYSARTEIGQTSRILVVEDEELIQEMLAVALEEEGYGVVTAADGRSAVEYLKSFDPNGGDFPFDLVILDL
MLPQINGLDICRLLRHQGNPVPILMLSAKGSETDRVLGLEVGADDYLTKPFSMRELVARCRALLRRQRLITLPQLPVLKF
KDVTLNPQECRVLVRGQEVNLSPKEFRLLELFMSYARRVWSREQLLDQVWGPDFVGDSKTVDVHIRWLREKLEQDPSHPE
YIVTVRGFGYRFG

Specific function: This Protein Is A Positive Regulator For The Phosphate Regulon. Transcription Of This Operon Is Positively Regulated By Phob And Phor When Phosphate Is Limited. [C]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=232, Percent_Identity=44.8275862068966, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1786911, Length=232, Percent_Identity=43.1034482758621, Blast_Score=175, Evalue=2e-45,
Organism=Escherichia coli, GI1789809, Length=238, Percent_Identity=40.3361344537815, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1786784, Length=228, Percent_Identity=39.4736842105263, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1790863, Length=239, Percent_Identity=38.0753138075314, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1788394, Length=237, Percent_Identity=38.3966244725738, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1790860, Length=231, Percent_Identity=38.0952380952381, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1789402, Length=231, Percent_Identity=39.3939393939394, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI2367329, Length=235, Percent_Identity=40.8510638297872, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI87082012, Length=230, Percent_Identity=37.8260869565217, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1790552, Length=228, Percent_Identity=38.1578947368421, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787375, Length=237, Percent_Identity=35.0210970464135, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787229, Length=236, Percent_Identity=35.1694915254237, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI145693140, Length=241, Percent_Identity=31.1203319502075, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787487, Length=122, Percent_Identity=35.2459016393443, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788550, Length=115, Percent_Identity=29.5652173913043, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28933; Mature: 28933

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISMYTSESIKYSARTEIGQTSRILVVEDEELIQEMLAVALEEEGYGVVTAADGRSAVEY
CCEEECCCCCCCHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
LKSFDPNGGDFPFDLVILDLMLPQINGLDICRLLRHQGNPVPILMLSAKGSETDRVLGLE
HHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEE
VGADDYLTKPFSMRELVARCRALLRRQRLITLPQLPVLKFKDVTLNPQECRVLVRGQEVN
ECCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHCEEEEECCCCC
LSPKEFRLLELFMSYARRVWSREQLLDQVWGPDFVGDSKTVDVHIRWLREKLEQDPSHPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCC
YIVTVRGFGYRFG
EEEEEECCCCCCC
>Mature Secondary Structure
MISMYTSESIKYSARTEIGQTSRILVVEDEELIQEMLAVALEEEGYGVVTAADGRSAVEY
CCEEECCCCCCCHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
LKSFDPNGGDFPFDLVILDLMLPQINGLDICRLLRHQGNPVPILMLSAKGSETDRVLGLE
HHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEE
VGADDYLTKPFSMRELVARCRALLRRQRLITLPQLPVLKFKDVTLNPQECRVLVRGQEVN
ECCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHCEEEEECCCCC
LSPKEFRLLELFMSYARRVWSREQLLDQVWGPDFVGDSKTVDVHIRWLREKLEQDPSHPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCC
YIVTVRGFGYRFG
EEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1554697 [H]