Click here to switch to the map view.

The map label for this gene is hepK [H]

Identifier: 17231988

GI number: 17231988

Start: 5384447

End: 5386174

Strand: Reverse

Name: hepK [H]

Synonym: all4496

Alternate gene names: 17231988

Gene position: 5386174-5384447 (Counterclockwise)

Preceding gene: 17231989

Following gene: 17231987

Centisome position: 83.98

GC content: 42.42

Gene sequence:

>1728_bases
ATGCAAACTCAGCAGCCGATCCCTGTTGACAGCAGTTCAGACAGCAAAGAAGTGTCATTAGAAGAGCCATCAACATCGAC
AGACGAACTCCCAACTATAGAATTTCCTTCACGAGGGAAACTCAAAGCAAGTTCCTGGCGTATCCATCAGAAAATTGGGT
ATGGGTACTTTGTGGCAATTGGAATTGGCTTTTTTGGCTCACTCACAGGATTAGTGATTGCCAACTACTACCGAGGCAAA
GAAATTAGGGAATTTAATCAAGCTCAAGAACAAAGACAATTATTGACTGATTACAAGAATGCTGTTGTTGGAGCGCAACT
GCATAGCTCTAATATTGTGGCATTTTTGGAAGATCCCCAGCGCTTACCGATGAAAAAAGCCGATTTCTTGAGGGATGTCA
AAAAAGCCGAAACTGATGAACAAAAAATTGCCAAGTTTATAGATGGTAGACCTAAAGACTTAGCTGCAACTAGTGCCAAC
TTAAAAGCTCTATTACAAGATTATGCGTTTTATTTAGATTCTTATGTGCAGCAAATAGATGCTGTCGTAGCAGAAATTCA
GCAGCCTGTACCACCAAAACAGGCAGCCGAAGTCAGAGCGAAGTTATTGGAGATTATGCGCGGCGAAACAGCCATGCAGC
TAGAGCAACTCTCACAGAAGTTATCCAATATCTTACAAACTGCTGAATCGAAAGAGCGTCAGAGACAAAGAGATGTTGAA
GAAGCCAAAAGCGTTGAGAGATTAATTGTGATGGTGAGTATGCTGGTGTCAGTGGCGATCGCTGCCATCGTAGCATGGCG
GACTAGTCGAGCGATCGCTGAACCAGTAATTATGGTCACCCAAGTAGCAGAACAAGTAGCTCGCAAACCAAATTTTGATT
TACGCGCTCCTGTCACGACAGAAGATGAAATCGGCTTACTGGCTAAATCCCTCAATCGTCTCATTGAGCGTGTATCTGAG
CGTACTAAAGAACTAGAACAGGCCAAAGAACTAGCTGAAGCAGCCAGCAAAGCAAAAAGTATATTTCTTGCCAATGTTAG
TCATGAGTTGCGTACACCTTTAAACGCTGTAATTGGCTTAAGCCAACTCCTCAAAGACGATGCGGCTGATCTGAATTTGT
CGGGAGAATTCACCAGTGATCTGGAAACCATCAATTCTGCGGGTAGACATCTACTGGAACTGATTAACGACATTCTGGAT
TTGTCAAAAATCGAAGCGGGTAAAATGACCCTTTATCCAGAGATATTTGATGTTGTTACACTCATCAATAATGTTGTTGT
AACCGTCAAACCGACTATAGAAAAAAATAACAACCTTCTAGAAATATATTGTGATGAACACCTGGGTACAATGTATGCTG
ATCAAACCAGGATGCGGCAAGTATTATTAAATCTACTCAGTAATGCTGCTAAATTTACCACTAACGGCAGAATCAAACTG
ACAGTTAGGCGAGAAAAAGAAGACTTTTTACCAGAAATGCCCTTTGGTGCGATCTCCTTCACAGTTAGCGATACAGGTAT
TGGGATGTCTCCAAGTCAGCAGCAACAGTTATTTCAACCCTTTACTCAAGGTGACACCTCAACCACGAAAAAATATGGCG
GTACTGGACTAGGTTTAGCAATTAGCCGCCACTTTTGCCAGATGATGGGAGGGGAAATTATCGTCAAAAGTCAACTGGGT
TTGGGTTCGACTTTTACCGTCTACCTGCCACTTCAGGAGCAAAGTTAA

Upstream 100 bases:

>100_bases
TTATCAGCCAAGATAATACAGGTGTTTATAAACATTTGTAGTGGTTATACAAATGAGGGTGGTTTAGTGGTTTTTGCGGG
AGGGTAAGGAGATTTGAGGG

Downstream 100 bases:

>100_bases
AGGTAGAGTAGTAGATGGGGAGGCCAGGAGAACAAATCATACCAATTCAAAATAGCCTACGGCAAGGCTGCGCCAACAAA
ATTCAAAATTAGGAAAGCCA

Product: two-component system sensory histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MQTQQPIPVDSSSDSKEVSLEEPSTSTDELPTIEFPSRGKLKASSWRIHQKIGYGYFVAIGIGFFGSLTGLVIANYYRGK
EIREFNQAQEQRQLLTDYKNAVVGAQLHSSNIVAFLEDPQRLPMKKADFLRDVKKAETDEQKIAKFIDGRPKDLAATSAN
LKALLQDYAFYLDSYVQQIDAVVAEIQQPVPPKQAAEVRAKLLEIMRGETAMQLEQLSQKLSNILQTAESKERQRQRDVE
EAKSVERLIVMVSMLVSVAIAAIVAWRTSRAIAEPVIMVTQVAEQVARKPNFDLRAPVTTEDEIGLLAKSLNRLIERVSE
RTKELEQAKELAEAASKAKSIFLANVSHELRTPLNAVIGLSQLLKDDAADLNLSGEFTSDLETINSAGRHLLELINDILD
LSKIEAGKMTLYPEIFDVVTLINNVVVTVKPTIEKNNNLLEIYCDEHLGTMYADQTRMRQVLLNLLSNAAKFTTNGRIKL
TVRREKEDFLPEMPFGAISFTVSDTGIGMSPSQQQQLFQPFTQGDTSTTKKYGGTGLGLAISRHFCQMMGGEIIVKSQLG
LGSTFTVYLPLQEQS

Sequences:

>Translated_575_residues
MQTQQPIPVDSSSDSKEVSLEEPSTSTDELPTIEFPSRGKLKASSWRIHQKIGYGYFVAIGIGFFGSLTGLVIANYYRGK
EIREFNQAQEQRQLLTDYKNAVVGAQLHSSNIVAFLEDPQRLPMKKADFLRDVKKAETDEQKIAKFIDGRPKDLAATSAN
LKALLQDYAFYLDSYVQQIDAVVAEIQQPVPPKQAAEVRAKLLEIMRGETAMQLEQLSQKLSNILQTAESKERQRQRDVE
EAKSVERLIVMVSMLVSVAIAAIVAWRTSRAIAEPVIMVTQVAEQVARKPNFDLRAPVTTEDEIGLLAKSLNRLIERVSE
RTKELEQAKELAEAASKAKSIFLANVSHELRTPLNAVIGLSQLLKDDAADLNLSGEFTSDLETINSAGRHLLELINDILD
LSKIEAGKMTLYPEIFDVVTLINNVVVTVKPTIEKNNNLLEIYCDEHLGTMYADQTRMRQVLLNLLSNAAKFTTNGRIKL
TVRREKEDFLPEMPFGAISFTVSDTGIGMSPSQQQQLFQPFTQGDTSTTKKYGGTGLGLAISRHFCQMMGGEIIVKSQLG
LGSTFTVYLPLQEQS
>Mature_575_residues
MQTQQPIPVDSSSDSKEVSLEEPSTSTDELPTIEFPSRGKLKASSWRIHQKIGYGYFVAIGIGFFGSLTGLVIANYYRGK
EIREFNQAQEQRQLLTDYKNAVVGAQLHSSNIVAFLEDPQRLPMKKADFLRDVKKAETDEQKIAKFIDGRPKDLAATSAN
LKALLQDYAFYLDSYVQQIDAVVAEIQQPVPPKQAAEVRAKLLEIMRGETAMQLEQLSQKLSNILQTAESKERQRQRDVE
EAKSVERLIVMVSMLVSVAIAAIVAWRTSRAIAEPVIMVTQVAEQVARKPNFDLRAPVTTEDEIGLLAKSLNRLIERVSE
RTKELEQAKELAEAASKAKSIFLANVSHELRTPLNAVIGLSQLLKDDAADLNLSGEFTSDLETINSAGRHLLELINDILD
LSKIEAGKMTLYPEIFDVVTLINNVVVTVKPTIEKNNNLLEIYCDEHLGTMYADQTRMRQVLLNLLSNAAKFTTNGRIKL
TVRREKEDFLPEMPFGAISFTVSDTGIGMSPSQQQQLFQPFTQGDTSTTKKYGGTGLGLAISRHFCQMMGGEIIVKSQLG
LGSTFTVYLPLQEQS

Specific function: At low cell density, in absence of autoinducer has a kinase activity, and autophosphorylates on a histidine residue. The phosphoryl group is then transferred to an aspartate residue in the response regulator domain. The phosphoryl group is transferred to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI87081816, Length=408, Percent_Identity=33.578431372549, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789149, Length=360, Percent_Identity=33.0555555555556, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI145693157, Length=260, Percent_Identity=39.2307692307692, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI48994928, Length=429, Percent_Identity=29.6037296037296, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788713, Length=254, Percent_Identity=36.6141732283465, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1786912, Length=271, Percent_Identity=29.1512915129151, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786600, Length=234, Percent_Identity=30.3418803418803, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788393, Length=306, Percent_Identity=25.8169934640523, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790436, Length=267, Percent_Identity=29.2134831460674, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790346, Length=235, Percent_Identity=29.3617021276596, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788549, Length=261, Percent_Identity=27.2030651340996, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786783, Length=324, Percent_Identity=24.3827160493827, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788545, Length=258, Percent_Identity=26.7441860465116, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87082128, Length=342, Percent_Identity=23.6842105263158, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790300, Length=255, Percent_Identity=27.843137254902, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787894, Length=241, Percent_Identity=25.7261410788382, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=76, Percent_Identity=50, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR015387
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789
- ProDom:   PD142495 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF09308 LuxQ-periplasm; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 63962; Mature: 63962

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCC
>Mature Secondary Structure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HHHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA