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The map label for this gene is pcrA [H]

Identifier: 17231987

GI number: 17231987

Start: 5381870

End: 5384188

Strand: Reverse

Name: pcrA [H]

Synonym: all4495

Alternate gene names: 17231987

Gene position: 5384188-5381870 (Counterclockwise)

Preceding gene: 17231988

Following gene: 17231975

Centisome position: 83.95

GC content: 41.14

Gene sequence:

>2319_bases
ATGACTACCGACTTTCTCAGTCATCTGAATCCCAGCCAACGTCAAGCCGTGGAACACTATTGCGGCCCCTTGTTAGTTGT
TGCTGGTGCTGGTTCCGGTAAAACACGAGCGTTAACTTATCGGATTGCTAATTTAATACTGAAGCACCGTGTTGCTCCCG
AAAATATTTTGGCGGTGACTTTCACCAACAAAGCCGCCAGGGAGATGAAAGAACGGATTCAACGGCTGTTTGCAGAACAA
TTAGCATTGACAGAACATGGAAAGCGTTTAGATTTACTACCAGAACACGAACAAACTAAATTGCGATCGCGTGTTTATAA
AACTACTATTAAAGAACTGTGGTGTGGTACTTTCCATAGTTTGTTTTCCCGTATTCTCCGCTTTGATATCGAAAAATACC
AAGATGAAAAAGGGCGACAATGGAACCGAAATTTTTCCATTTTTGATGAATCTGATGTACAGGGTCTCATCAAAGAAATC
GTCACTAAACAATTAAATCTCGATGATAAGAAGTTTGAGCCTCGTTCTGTCCGCTACGCCATTAGTAATGCAAAAAATCA
AGGACTTTCACCCCAGGAATTTGAAAAAGACCAACCAAATTATCGTGGGAGAGTAATTGCTGATGTCTATAATCGCTATC
AAGATAAACTAGCAGAAAACAACGCTCTTGATTTTGATGATTTAATTTTAATTCCTACGAGATTATTTCAACAAAATGAG
CAGGTTTTAGGTTACTGGCATCGCAAGTTCTGTCATATTCTTGTAGACGAATATCAAGATACTAACCGCACTCAATATGA
CCTAATTCGTCTGTTGACAACTAATGGCGAAGAGAAAAAAAGTGCATGGGAGTGGCAAAATCGCTCTGTTTTTGTTGTAG
GTGATGCAGACCAATCTATTTACAGCTTTAGAATGGCAGATTTCACCATATTACTGGATTTTCAGAATCAGTTTGGTGAT
GGTTTGCCAGATGAAGACACCCGCACAATGGTGAAGTTGGAAGAAAACTATCGTTCTTGTGAAAACATTCTCCAAGTTGC
TAACGAACTAATTGAAAATAATACTCAAAGAATCGATAAAGTCCTCAAACCAACGCGGGGAGCAGGCGAAGAAATTTACT
GTCACAAGGCAGATAATGAATTAGAAGAAGCTGATTTTGTAATTCGTCAAATCCGCACATTAGAACATCAAAACCCGGAA
TTGGATTGGGGAAGCTTTGCAATTTTGTATCGGACAAATGCCCAATCTCGTCCCTTTGAAGAATTGTTAGTGAGGAATCA
GATTCCTTACACTGTTGTGGGTGGGATGAAATTCTATGACCGCAAAGAAATTAAAGATGTTATAGCTTATTTAAGAGCGA
TCTCTAACCCCTCGGATACAGTTAGTTTATTACGAGTAATCAACACCCCTCGGCGCGGTATTGGTAAAGCAACTATTGAT
AACTTAATCAATGCTTCCCAACAATTAGGTACTACTCTCTGGGAAATCTTAAGTGATGAAACATCAGTCAATACATTGGC
TGGGCGATCGGCAAAAGGGGTGAATAACTTTGCCAAAATGATTAACCGTTGGCAAGAACAAATAGCTACAGCCCCAGTTA
CAGAAATAGTTATGGGGATTTTAGAAGATTCTGGTTACGTACAAGATTTACAAAGCCAAGGAACAGACGAATCTGAAGAC
CGCATCCAAAACGTACAGGAACTTTATAACGCTGTCTTGCAATTCCAAGAAGAGAATGAAGATGTTTCTTTAACAGCTTT
CCTGCAAAGTACAGCCCTTAGTTCTGATTTAGATAACCTCAAGGAAGGTCAAACAGCCGTTTCCCTCATGACTCTGCACG
CTTCCAAAGGTTTGGAATTTCCTGTAGTCTTCTTGGTAGGTTTAGAACAGGGATTATTCCCCGGCTACCGTTCACTAAGT
GACCCCGCAGCCTTAGAAGAAGAGCGTCGCCTCTGTTATGTAGGGATTACTCGCGCCCAAGAACGGCTGTATTTATCCTT
CGCCCGTGAACGCCGCTTATACGGCTCACGAGAACCCGCCATGCGATCACAATTTCTCGATGAATTACCAGCCGAATTAA
TCACAACCCAATACCAAGTCCGGCAAACTTATACTAGACCCCCATCTACTACCAATGGTAAAGAAACAGCCAATCAAGAT
TGGCAAGTAGGGGACAGAGTATTACACAAAAGTTTTGGGATTGGGGAAATAACCCACGTTTTTGGTGCTGGTAGCAAGCT
TTCTGTGGCTATTAAATTTACGAGCTTGGGTCAGAAAATTGTTGACCCTAGAGTCGCCCAATTACAAAAGGTGGATTAG

Upstream 100 bases:

>100_bases
CATTAATTGTTGACTACTGACTACTGACTACTGACTACTGACTACTGACTACTGACTACTGACTACTGACTACTGACTAC
TGACCACTGACTAATAACTA

Downstream 100 bases:

>100_bases
TCATTAGTCAATAGTCAATGGTCAATAGTTTTTCTTCCTCTCGTCCCCAGTCCCTAATTCAAAGATAACGAGATAAGACT
TCACTCGTTGCTTTACCTGT

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 772; Mature: 771

Protein sequence:

>772_residues
MTTDFLSHLNPSQRQAVEHYCGPLLVVAGAGSGKTRALTYRIANLILKHRVAPENILAVTFTNKAAREMKERIQRLFAEQ
LALTEHGKRLDLLPEHEQTKLRSRVYKTTIKELWCGTFHSLFSRILRFDIEKYQDEKGRQWNRNFSIFDESDVQGLIKEI
VTKQLNLDDKKFEPRSVRYAISNAKNQGLSPQEFEKDQPNYRGRVIADVYNRYQDKLAENNALDFDDLILIPTRLFQQNE
QVLGYWHRKFCHILVDEYQDTNRTQYDLIRLLTTNGEEKKSAWEWQNRSVFVVGDADQSIYSFRMADFTILLDFQNQFGD
GLPDEDTRTMVKLEENYRSCENILQVANELIENNTQRIDKVLKPTRGAGEEIYCHKADNELEEADFVIRQIRTLEHQNPE
LDWGSFAILYRTNAQSRPFEELLVRNQIPYTVVGGMKFYDRKEIKDVIAYLRAISNPSDTVSLLRVINTPRRGIGKATID
NLINASQQLGTTLWEILSDETSVNTLAGRSAKGVNNFAKMINRWQEQIATAPVTEIVMGILEDSGYVQDLQSQGTDESED
RIQNVQELYNAVLQFQEENEDVSLTAFLQSTALSSDLDNLKEGQTAVSLMTLHASKGLEFPVVFLVGLEQGLFPGYRSLS
DPAALEEERRLCYVGITRAQERLYLSFARERRLYGSREPAMRSQFLDELPAELITTQYQVRQTYTRPPSTTNGKETANQD
WQVGDRVLHKSFGIGEITHVFGAGSKLSVAIKFTSLGQKIVDPRVAQLQKVD

Sequences:

>Translated_772_residues
MTTDFLSHLNPSQRQAVEHYCGPLLVVAGAGSGKTRALTYRIANLILKHRVAPENILAVTFTNKAAREMKERIQRLFAEQ
LALTEHGKRLDLLPEHEQTKLRSRVYKTTIKELWCGTFHSLFSRILRFDIEKYQDEKGRQWNRNFSIFDESDVQGLIKEI
VTKQLNLDDKKFEPRSVRYAISNAKNQGLSPQEFEKDQPNYRGRVIADVYNRYQDKLAENNALDFDDLILIPTRLFQQNE
QVLGYWHRKFCHILVDEYQDTNRTQYDLIRLLTTNGEEKKSAWEWQNRSVFVVGDADQSIYSFRMADFTILLDFQNQFGD
GLPDEDTRTMVKLEENYRSCENILQVANELIENNTQRIDKVLKPTRGAGEEIYCHKADNELEEADFVIRQIRTLEHQNPE
LDWGSFAILYRTNAQSRPFEELLVRNQIPYTVVGGMKFYDRKEIKDVIAYLRAISNPSDTVSLLRVINTPRRGIGKATID
NLINASQQLGTTLWEILSDETSVNTLAGRSAKGVNNFAKMINRWQEQIATAPVTEIVMGILEDSGYVQDLQSQGTDESED
RIQNVQELYNAVLQFQEENEDVSLTAFLQSTALSSDLDNLKEGQTAVSLMTLHASKGLEFPVVFLVGLEQGLFPGYRSLS
DPAALEEERRLCYVGITRAQERLYLSFARERRLYGSREPAMRSQFLDELPAELITTQYQVRQTYTRPPSTTNGKETANQD
WQVGDRVLHKSFGIGEITHVFGAGSKLSVAIKFTSLGQKIVDPRVAQLQKVD
>Mature_771_residues
TTDFLSHLNPSQRQAVEHYCGPLLVVAGAGSGKTRALTYRIANLILKHRVAPENILAVTFTNKAAREMKERIQRLFAEQL
ALTEHGKRLDLLPEHEQTKLRSRVYKTTIKELWCGTFHSLFSRILRFDIEKYQDEKGRQWNRNFSIFDESDVQGLIKEIV
TKQLNLDDKKFEPRSVRYAISNAKNQGLSPQEFEKDQPNYRGRVIADVYNRYQDKLAENNALDFDDLILIPTRLFQQNEQ
VLGYWHRKFCHILVDEYQDTNRTQYDLIRLLTTNGEEKKSAWEWQNRSVFVVGDADQSIYSFRMADFTILLDFQNQFGDG
LPDEDTRTMVKLEENYRSCENILQVANELIENNTQRIDKVLKPTRGAGEEIYCHKADNELEEADFVIRQIRTLEHQNPEL
DWGSFAILYRTNAQSRPFEELLVRNQIPYTVVGGMKFYDRKEIKDVIAYLRAISNPSDTVSLLRVINTPRRGIGKATIDN
LINASQQLGTTLWEILSDETSVNTLAGRSAKGVNNFAKMINRWQEQIATAPVTEIVMGILEDSGYVQDLQSQGTDESEDR
IQNVQELYNAVLQFQEENEDVSLTAFLQSTALSSDLDNLKEGQTAVSLMTLHASKGLEFPVVFLVGLEQGLFPGYRSLSD
PAALEEERRLCYVGITRAQERLYLSFARERRLYGSREPAMRSQFLDELPAELITTQYQVRQTYTRPPSTTNGKETANQDW
QVGDRVLHKSFGIGEITHVFGAGSKLSVAIKFTSLGQKIVDPRVAQLQKVD

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=776, Percent_Identity=38.4020618556701, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI48994965, Length=694, Percent_Identity=38.328530259366, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1787196, Length=369, Percent_Identity=28.7262872628726, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=777, Percent_Identity=28.4427284427284, Blast_Score=234, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6324477, Length=739, Percent_Identity=24.4925575101488, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 88547; Mature: 88416

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDFLSHLNPSQRQAVEHYCGPLLVVAGAGSGKTRALTYRIANLILKHRVAPENILAVT
CCCHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
FTNKAAREMKERIQRLFAEQLALTEHGKRLDLLPEHEQTKLRSRVYKTTIKELWCGTFHS
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LFSRILRFDIEKYQDEKGRQWNRNFSIFDESDVQGLIKEIVTKQLNLDDKKFEPRSVRYA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
ISNAKNQGLSPQEFEKDQPNYRGRVIADVYNRYQDKLAENNALDFDDLILIPTRLFQQNE
HHCCCCCCCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCH
QVLGYWHRKFCHILVDEYQDTNRTQYDLIRLLTTNGEEKKSAWEWQNRSVFVVGDADQSI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCHH
YSFRMADFTILLDFQNQFGDGLPDEDTRTMVKLEENYRSCENILQVANELIENNTQRIDK
HHHHHCCEEEEEEECHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VLKPTRGAGEEIYCHKADNELEEADFVIRQIRTLEHQNPELDWGSFAILYRTNAQSRPFE
HHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHH
ELLVRNQIPYTVVGGMKFYDRKEIKDVIAYLRAISNPSDTVSLLRVINTPRRGIGKATID
HHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHH
NLINASQQLGTTLWEILSDETSVNTLAGRSAKGVNNFAKMINRWQEQIATAPVTEIVMGI
HHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
LEDSGYVQDLQSQGTDESEDRIQNVQELYNAVLQFQEENEDVSLTAFLQSTALSSDLDNL
HCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
KEGQTAVSLMTLHASKGLEFPVVFLVGLEQGLFPGYRSLSDPAALEEERRLCYVGITRAQ
HCCHHHHHHHHHHHCCCCCCHHHEEEECHHCCCCCCCCCCCCHHHHHCCCEEEEEHHHHH
ERLYLSFARERRLYGSREPAMRSQFLDELPAELITTQYQVRQTYTRPPSTTNGKETANQD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
WQVGDRVLHKSFGIGEITHVFGAGSKLSVAIKFTSLGQKIVDPRVAQLQKVD
HHHHHHHHHHCCCCCHHHHHHCCCCEEEEEEEEHHHCHHHCCCHHHHHHCCC
>Mature Secondary Structure 
TTDFLSHLNPSQRQAVEHYCGPLLVVAGAGSGKTRALTYRIANLILKHRVAPENILAVT
CCHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
FTNKAAREMKERIQRLFAEQLALTEHGKRLDLLPEHEQTKLRSRVYKTTIKELWCGTFHS
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LFSRILRFDIEKYQDEKGRQWNRNFSIFDESDVQGLIKEIVTKQLNLDDKKFEPRSVRYA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
ISNAKNQGLSPQEFEKDQPNYRGRVIADVYNRYQDKLAENNALDFDDLILIPTRLFQQNE
HHCCCCCCCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCH
QVLGYWHRKFCHILVDEYQDTNRTQYDLIRLLTTNGEEKKSAWEWQNRSVFVVGDADQSI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCHH
YSFRMADFTILLDFQNQFGDGLPDEDTRTMVKLEENYRSCENILQVANELIENNTQRIDK
HHHHHCCEEEEEEECHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VLKPTRGAGEEIYCHKADNELEEADFVIRQIRTLEHQNPELDWGSFAILYRTNAQSRPFE
HHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHH
ELLVRNQIPYTVVGGMKFYDRKEIKDVIAYLRAISNPSDTVSLLRVINTPRRGIGKATID
HHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHH
NLINASQQLGTTLWEILSDETSVNTLAGRSAKGVNNFAKMINRWQEQIATAPVTEIVMGI
HHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
LEDSGYVQDLQSQGTDESEDRIQNVQELYNAVLQFQEENEDVSLTAFLQSTALSSDLDNL
HCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
KEGQTAVSLMTLHASKGLEFPVVFLVGLEQGLFPGYRSLSDPAALEEERRLCYVGITRAQ
HCCHHHHHHHHHHHCCCCCCHHHEEEECHHCCCCCCCCCCCCHHHHHCCCEEEEEHHHHH
ERLYLSFARERRLYGSREPAMRSQFLDELPAELITTQYQVRQTYTRPPSTTNGKETANQD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
WQVGDRVLHKSFGIGEITHVFGAGSKLSVAIKFTSLGQKIVDPRVAQLQKVD
HHHHHHHHHHCCCCCHHHHHHCCCCEEEEEEEEHHHCHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]