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The map label for this gene is rfbD [H]

Identifier: 17231982

GI number: 17231982

Start: 5376652

End: 5377536

Strand: Direct

Name: rfbD [H]

Synonym: alr4490

Alternate gene names: 17231982

Gene position: 5376652-5377536 (Clockwise)

Preceding gene: 17231981

Following gene: 17231983

Centisome position: 83.83

GC content: 44.18

Gene sequence:

>885_bases
ATGAATGAACCAATCCTACTACTAGGTAGCAACGGTCAAGTAGGTCAAGAAATCCAAAATCTCCTTGCACCTAGATACAA
AATTATCTCAGTCGCACGACCAAGGATAGACCTGACTCAAGCCGATAGTCTGCGTCAAATCATCAGAGAAGTCCAACCAC
AGATAATTATTAACGCCGCCGCTTACACTGCTGTAGACAAAGCCGAAACTGAACCCGAAATAGCTACAGCTATCAATGCT
ACAGCACCCCAAATTATCGCCGAAGAAAGCCAAAAACTCGGCTCGTTCTTAATTCACATATCCACAGATTACGTTTTTGA
TGGTCAGCAAAGTCACCCTTACCAAGAAACTGACCCCACTAACCCCTTAAGTGTTTATGGTAAGACCAAACTAGCCGGAG
AAATAGCCATTCAACAAACTCACCCTCATCACATTATTCTGCGTACAGCTTGGGTTTATGGCAGTTTTGGTAAAAGTAAC
TTTGTCAAAACCATGCTGCGACTGGGTGCAGAACGCCAAGAAATTCGGGTAGTAAAAGATCAAATTGGCTCCCCTACTTG
GGCGCAAGATATCGCTGACACCATAGCCCAAGTGATACCCCAAATACCAGAAATTTCTGGGACTTACCACTACACAAATA
CTGGTGTAATTAGCTGGTACGAATTTGCTGTTGCTATTTTTGCAGAATCTCAAAAACTAGGTTTTCCTCTCACAGTCCAA
CAAATTATTCCTATTACCACCGCCGAATATCCGACTTTAGCTCCCCGTCCTGCCTATTCTGTCCTGGCTTGTGAGAAGAT
GTCACAAGTTCTGGGAACTGCTCCCCCTCATTGGCAACAAAGACTGCGGTTGATGCTGCAAGACTGGCTCTCCAAATATT
CTTGA

Upstream 100 bases:

>100_bases
TGGAATGATCCTGATTTAGCAATAAATTGGCCTCTCAAAGAACCACCAATTTTATCAGCCAAAGATAGCCAGGGTCAGCC
ATTTAAAAGTGCGGAAGTAT

Downstream 100 bases:

>100_bases
TTATGAAAGCACTAATTCTCTCTGGCGGTAGAGGTACACGTCTACGTCCACTCACCTATACTGGAGCAAAGCAACTTGTC
CCAGTTGCGAACAAGCCTAT

Product: dTDP-6-deoxy-L-mannose-dehydrogenase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MNEPILLLGSNGQVGQEIQNLLAPRYKIISVARPRIDLTQADSLRQIIREVQPQIIINAAAYTAVDKAETEPEIATAINA
TAPQIIAEESQKLGSFLIHISTDYVFDGQQSHPYQETDPTNPLSVYGKTKLAGEIAIQQTHPHHIILRTAWVYGSFGKSN
FVKTMLRLGAERQEIRVVKDQIGSPTWAQDIADTIAQVIPQIPEISGTYHYTNTGVISWYEFAVAIFAESQKLGFPLTVQ
QIIPITTAEYPTLAPRPAYSVLACEKMSQVLGTAPPHWQQRLRLMLQDWLSKYS

Sequences:

>Translated_294_residues
MNEPILLLGSNGQVGQEIQNLLAPRYKIISVARPRIDLTQADSLRQIIREVQPQIIINAAAYTAVDKAETEPEIATAINA
TAPQIIAEESQKLGSFLIHISTDYVFDGQQSHPYQETDPTNPLSVYGKTKLAGEIAIQQTHPHHIILRTAWVYGSFGKSN
FVKTMLRLGAERQEIRVVKDQIGSPTWAQDIADTIAQVIPQIPEISGTYHYTNTGVISWYEFAVAIFAESQKLGFPLTVQ
QIIPITTAEYPTLAPRPAYSVLACEKMSQVLGTAPPHWQQRLRLMLQDWLSKYS
>Mature_294_residues
MNEPILLLGSNGQVGQEIQNLLAPRYKIISVARPRIDLTQADSLRQIIREVQPQIIINAAAYTAVDKAETEPEIATAINA
TAPQIIAEESQKLGSFLIHISTDYVFDGQQSHPYQETDPTNPLSVYGKTKLAGEIAIQQTHPHHIILRTAWVYGSFGKSN
FVKTMLRLGAERQEIRVVKDQIGSPTWAQDIADTIAQVIPQIPEISGTYHYTNTGVISWYEFAVAIFAESQKLGFPLTVQ
QIIPITTAEYPTLAPRPAYSVLACEKMSQVLGTAPPHWQQRLRLMLQDWLSKYS

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=286, Percent_Identity=31.8181818181818, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI33519455, Length=286, Percent_Identity=31.8181818181818, Blast_Score=121, Evalue=6e-28,
Organism=Escherichia coli, GI1788352, Length=291, Percent_Identity=44.3298969072165, Blast_Score=254, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32790; Mature: 32790

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEPILLLGSNGQVGQEIQNLLAPRYKIISVARPRIDLTQADSLRQIIREVQPQIIINAA
CCCCEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEHH
AYTAVDKAETEPEIATAINATAPQIIAEESQKLGSFLIHISTDYVFDGQQSHPYQETDPT
HHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCEEECCCCCCCCCCCCCC
NPLSVYGKTKLAGEIAIQQTHPHHIILRTAWVYGSFGKSNFVKTMLRLGAERQEIRVVKD
CCEEECCCCEECEEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHH
QIGSPTWAQDIADTIAQVIPQIPEISGTYHYTNTGVISWYEFAVAIFAESQKLGFPLTVQ
HCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEHH
QIIPITTAEYPTLAPRPAYSVLACEKMSQVLGTAPPHWQQRLRLMLQDWLSKYS
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNEPILLLGSNGQVGQEIQNLLAPRYKIISVARPRIDLTQADSLRQIIREVQPQIIINAA
CCCCEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEHH
AYTAVDKAETEPEIATAINATAPQIIAEESQKLGSFLIHISTDYVFDGQQSHPYQETDPT
HHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCEEECCCCCCCCCCCCCC
NPLSVYGKTKLAGEIAIQQTHPHHIILRTAWVYGSFGKSNFVKTMLRLGAERQEIRVVKD
CCEEECCCCEECEEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHH
QIGSPTWAQDIADTIAQVIPQIPEISGTYHYTNTGVISWYEFAVAIFAESQKLGFPLTVQ
HCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEHH
QIIPITTAEYPTLAPRPAYSVLACEKMSQVLGTAPPHWQQRLRLMLQDWLSKYS
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]