Click here to switch to the map view.

The map label for this gene is ntcA

Identifier: 17231884

GI number: 17231884

Start: 5265413

End: 5266084

Strand: Direct

Name: ntcA

Synonym: alr4392

Alternate gene names: 17231884

Gene position: 5265413-5266084 (Clockwise)

Preceding gene: 17231878

Following gene: 17231885

Centisome position: 82.1

GC content: 43.9

Gene sequence:

>672_bases
ATGATCGTGACACAAGATAAGGCCCTAGCAAATGTTTTTCGTCAGATGGCAACCGGAGCTTTTCCTCCTGTTGTCGAAAC
GTTTGAACGCAATAAAACGATCTTTTTTCCTGGCGATCCTGCCGAACGAGTCTACTTTCTTTTGAAAGGGGCTGTGAAAC
TTTCCAGGGTGTACGAGGCAGGAGAAGAGATTACAGTAGCACTACTACGGGAAAATAGCGTTTTTGGTGTCCTGTCTTTG
TTGACAGGAAACAAGTCGGATAGGTTTTACCATGCGGTGGCATTTACTCCAGTAGAATTGCTTTCTGCACCAATTGAACA
AGTGGAGCAAGCACTGAAGGAAAATCCTGAATTATCGATGTTGATGCTGCGGGGTCTGTCTTCGCGGATTCTACAAACAG
AGATGATGATTGAAACCTTAGCGCACCGAGATATGGGTTCGAGATTGGTGAGTTTTCTGTTAATTCTCTGTCGTGATTTT
GGTGTTCCTTGTGCAGATGGAATCACAATTGATTTAAAGTTATCTCATCAGGCGATCGCCGAAGCAATTGGCTCTACTCG
CGTTACTGTTACTAGGCTACTAGGGGATTTGCGGGAGAAAAAGATGATTTCCATCCACAAAAAGAAGATTACTGTGCATA
AACCTGTGACTCTCAGCAGACAGTTCACTTAA

Upstream 100 bases:

>100_bases
GTACAGAAAGGTTAACGGTGCTTTATTGATTTTTCAGGTGTATTCTTAGGTAATTAGTTTTTAGTTTTTTGTTATTCCGG
CATTGGGTAGGGGAAGGGAG

Downstream 100 bases:

>100_bases
AGAAGAATAGCAGGAGTCAGAATTCAGAATGTGGCTAACTATTAGCACAGGCTAAATCCTACTCATTATAGATTTAGTTG
GTTTCTTCAATCCGTTTATT

Product: nitrogen-responsive regulatory protein

Products: NA

Alternate protein names: DNA-binding protein VF1; Nitrogen-responsive regulatory protein

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MIVTQDKALANVFRQMATGAFPPVVETFERNKTIFFPGDPAERVYFLLKGAVKLSRVYEAGEEITVALLRENSVFGVLSL
LTGNKSDRFYHAVAFTPVELLSAPIEQVEQALKENPELSMLMLRGLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRDF
GVPCADGITIDLKLSHQAIAEAIGSTRVTVTRLLGDLREKKMISIHKKKITVHKPVTLSRQFT

Sequences:

>Translated_223_residues
MIVTQDKALANVFRQMATGAFPPVVETFERNKTIFFPGDPAERVYFLLKGAVKLSRVYEAGEEITVALLRENSVFGVLSL
LTGNKSDRFYHAVAFTPVELLSAPIEQVEQALKENPELSMLMLRGLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRDF
GVPCADGITIDLKLSHQAIAEAIGSTRVTVTRLLGDLREKKMISIHKKKITVHKPVTLSRQFT
>Mature_223_residues
MIVTQDKALANVFRQMATGAFPPVVETFERNKTIFFPGDPAERVYFLLKGAVKLSRVYEAGEEITVALLRENSVFGVLSL
LTGNKSDRFYHAVAFTPVELLSAPIEQVEQALKENPELSMLMLRGLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRDF
GVPCADGITIDLKLSHQAIAEAIGSTRVTVTRLLGDLREKKMISIHKKKITVHKPVTLSRQFT

Specific function: Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3'

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789756, Length=193, Percent_Identity=27.4611398963731, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NTCA_ANAVT (P0A4U7)

Other databases:

- EMBL:   U89516
- EMBL:   CP000117
- RefSeq:   YP_323786.1
- ProteinModelPortal:   P0A4U7
- SMR:   P0A4U7
- STRING:   P0A4U7
- GeneID:   3680314
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_3283
- eggNOG:   COG0664
- HOGENOM:   HBG285314
- OMA:   VEMITAP
- PhylomeDB:   P0A4U7
- ProtClustDB:   CLSK893081
- BioCyc:   AVAR240292:AVA_3283-MONOMER
- GO:   GO:0005622
- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.60.120.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00034
- SMART:   SM00100
- SMART:   SM00419
- TIGRFAMs:   TIGR03697

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp; SSF51206 cNMP_binding

EC number: NA

Molecular weight: Translated: 24919; Mature: 24919

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS50042 CNMP_BINDING_3; PS00042 HTH_CRP_1; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTQDKALANVFRQMATGAFPPVVETFERNKTIFFPGDPAERVYFLLKGAVKLSRVYEA
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHC
GEEITVALLRENSVFGVLSLLTGNKSDRFYHAVAFTPVELLSAPIEQVEQALKENPELSM
CCCEEEEEEECCCHHHHHHHHHCCCCCCEEEEEHHCHHHHHHHHHHHHHHHHHCCCCHHH
LMLRGLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRDFGVPCADGITIDLKLSHQAIA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHH
EAIGSTRVTVTRLLGDLREKKMISIHKKKITVHKPVTLSRQFT
HHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCEECCCCCC
>Mature Secondary Structure
MIVTQDKALANVFRQMATGAFPPVVETFERNKTIFFPGDPAERVYFLLKGAVKLSRVYEA
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHC
GEEITVALLRENSVFGVLSLLTGNKSDRFYHAVAFTPVELLSAPIEQVEQALKENPELSM
CCCEEEEEEECCCHHHHHHHHHCCCCCCEEEEEHHCHHHHHHHHHHHHHHHHHCCCCHHH
LMLRGLSSRILQTEMMIETLAHRDMGSRLVSFLLILCRDFGVPCADGITIDLKLSHQAIA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHH
EAIGSTRVTVTRLLGDLREKKMISIHKKKITVHKPVTLSRQFT
HHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11114927