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The map label for this gene is cls [H]

Identifier: 17231715

GI number: 17231715

Start: 5059332

End: 5060774

Strand: Direct

Name: cls [H]

Synonym: alr4223

Alternate gene names: 17231715

Gene position: 5059332-5060774 (Clockwise)

Preceding gene: 17231714

Following gene: 17231716

Centisome position: 78.88

GC content: 39.29

Gene sequence:

>1443_bases
ATGCTGATAGACAGTAGCGTCCTTGCTTTTTTTAGCGCAGCTACAGTTGTTATTCATGGTTTGGGAATTGCCCATGCTGC
CCATGCCGTGATGGATGTGCGTTCTTCTCGTGGGGCGATCGCTTGGAGTATTTCCTTAATCACTTTTCCCTGGCTGACTA
TTCCTTTATATTGGATCTTAGGCAGAACTAAGTTTCATGGATATTCTGAAGCTATCCGCTCAGTTTACGCCCAACATTAT
CAATTTGTTCGTCAAACTTATATTGAGATTACAAAATTTCAGGTGGTAGTTTCGGCAAAATTGGAACCATTGCAACTATT
AGCTGAGGCCTTCATTGGTATTCCTTTTACATCTGGTAATAATGCCAAATTACTAATTAATGGTCAGCAAACTTATGCAG
CCATGTTGAGTGCGATCGCATCTGCCAATAGTTATATTTTGCTGCAATCCTACATTGTTGTTGATGACAAAGCTGGGAAT
GAGTTTAAAGATGCTCTAATTGCCAAGGCAAAGCAAGGAATAAGAGTTTACTTAATTTATGATGAAATTGGTTCTAATAA
AATCTCTCGCCTTTATGTTAAATCTCTGCAAAAATATGATATTCAAGTGAGTGCATTTCATACTACCAGAGGCAGAGGTA
ATCGTTTTCAATTAAATTTCCGCAACCATCGCAAAATTTTAGTAGTTGATGGGAGAACTGCGTTTATTGGTGGTTTGAAT
ATTAGTGATGAATATCTAGGTAAAAATCCTCGCCTGAGTCCTTGGCGTGATACCCACATGATGTTAGAAGGCCCCACTGT
CCAAAGTCTGCAAGGATGTTTTTTGCAAGATTGGTATTGGGCGACTCGTCAAGTTATCGATGTAAACTGGCAAGTGCAAC
CCAATTGGGAAAGTGACTACACAGCATTGGTATTTCCTACAGGCCCCGCAGATAAGCTGAAAGCTTGTAAACTGTTTTTC
GTCAGCGCTATCAATCAAGCACAAACTCGTCTCTGGATTGCTACTCCCTATTTTGTGCCGGATGATTCCACTTTGACAGC
CTTGAAATTGGCAGCACTACGCGGCGTGGATGTGCGAATTATTTTACCAAATCGACCCGATCATTTACTTGTGTACTTGT
GTTCCTTTTCTTATTACACAGAGATGAAGGCAACGAATATTAAATTATATCGATATAAACATGGATTTATGCACCAGAAG
GTAATACTGATTGACAAAGAAATGGCAGGTGTGGGAACAGTTAATCTAGATAATCGCTCATTCTTTCTCAATTTTGAAGT
TATGGGTTTTGTTGCCAATTCTCAGTTTGTGAAAAGTGTGGAGGAGATGTTACAAGCTGATTTAAAGGCTGCTTTGGCTG
TGGATTTTTCTGATTATGAGAGGAAATATTTGTGGTTTAAGTTAGCTGTGAGAATATCTAGTTTGTTGAAACCTTTGTTG
TGA

Upstream 100 bases:

>100_bases
TGAAAAACCAGCAACTGCTAAGGTTTTAGATGAAATTTGTTCATCCGATCACAAACCTTTACTGGCAGAATTTACTTATA
TCGATACATAATAAATTCAG

Downstream 100 bases:

>100_bases
GAGTCATGTAGCACTTTCCAGGCATAGCATATTATTTTACTTTCCAGTCACTCTCTGCTATCAAAAATCTATCTCTACCT
GAACGTTCTTGCGGTATTGG

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLIDSSVLAFFSAATVVIHGLGIAHAAHAVMDVRSSRGAIAWSISLITFPWLTIPLYWILGRTKFHGYSEAIRSVYAQHY
QFVRQTYIEITKFQVVVSAKLEPLQLLAEAFIGIPFTSGNNAKLLINGQQTYAAMLSAIASANSYILLQSYIVVDDKAGN
EFKDALIAKAKQGIRVYLIYDEIGSNKISRLYVKSLQKYDIQVSAFHTTRGRGNRFQLNFRNHRKILVVDGRTAFIGGLN
ISDEYLGKNPRLSPWRDTHMMLEGPTVQSLQGCFLQDWYWATRQVIDVNWQVQPNWESDYTALVFPTGPADKLKACKLFF
VSAINQAQTRLWIATPYFVPDDSTLTALKLAALRGVDVRIILPNRPDHLLVYLCSFSYYTEMKATNIKLYRYKHGFMHQK
VILIDKEMAGVGTVNLDNRSFFLNFEVMGFVANSQFVKSVEEMLQADLKAALAVDFSDYERKYLWFKLAVRISSLLKPLL

Sequences:

>Translated_480_residues
MLIDSSVLAFFSAATVVIHGLGIAHAAHAVMDVRSSRGAIAWSISLITFPWLTIPLYWILGRTKFHGYSEAIRSVYAQHY
QFVRQTYIEITKFQVVVSAKLEPLQLLAEAFIGIPFTSGNNAKLLINGQQTYAAMLSAIASANSYILLQSYIVVDDKAGN
EFKDALIAKAKQGIRVYLIYDEIGSNKISRLYVKSLQKYDIQVSAFHTTRGRGNRFQLNFRNHRKILVVDGRTAFIGGLN
ISDEYLGKNPRLSPWRDTHMMLEGPTVQSLQGCFLQDWYWATRQVIDVNWQVQPNWESDYTALVFPTGPADKLKACKLFF
VSAINQAQTRLWIATPYFVPDDSTLTALKLAALRGVDVRIILPNRPDHLLVYLCSFSYYTEMKATNIKLYRYKHGFMHQK
VILIDKEMAGVGTVNLDNRSFFLNFEVMGFVANSQFVKSVEEMLQADLKAALAVDFSDYERKYLWFKLAVRISSLLKPLL
>Mature_480_residues
MLIDSSVLAFFSAATVVIHGLGIAHAAHAVMDVRSSRGAIAWSISLITFPWLTIPLYWILGRTKFHGYSEAIRSVYAQHY
QFVRQTYIEITKFQVVVSAKLEPLQLLAEAFIGIPFTSGNNAKLLINGQQTYAAMLSAIASANSYILLQSYIVVDDKAGN
EFKDALIAKAKQGIRVYLIYDEIGSNKISRLYVKSLQKYDIQVSAFHTTRGRGNRFQLNFRNHRKILVVDGRTAFIGGLN
ISDEYLGKNPRLSPWRDTHMMLEGPTVQSLQGCFLQDWYWATRQVIDVNWQVQPNWESDYTALVFPTGPADKLKACKLFF
VSAINQAQTRLWIATPYFVPDDSTLTALKLAALRGVDVRIILPNRPDHLLVYLCSFSYYTEMKATNIKLYRYKHGFMHQK
VILIDKEMAGVGTVNLDNRSFFLNFEVMGFVANSQFVKSVEEMLQADLKAALAVDFSDYERKYLWFKLAVRISSLLKPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=461, Percent_Identity=27.9826464208243, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787007, Length=346, Percent_Identity=29.4797687861272, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87081826, Length=380, Percent_Identity=24.7368421052632, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 54496; Mature: 54496

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDSSVLAFFSAATVVIHGLGIAHAAHAVMDVRSSRGAIAWSISLITFPWLTIPLYWIL
CEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHH
GRTKFHGYSEAIRSVYAQHYQFVRQTYIEITKFQVVVSAKLEPLQLLAEAFIGIPFTSGN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHCCEEECCC
NAKLLINGQQTYAAMLSAIASANSYILLQSYIVVDDKAGNEFKDALIAKAKQGIRVYLIY
CCEEEEECCHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEE
DEIGSNKISRLYVKSLQKYDIQVSAFHTTRGRGNRFQLNFRNHRKILVVDGRTAFIGGLN
ECCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCEEEEEECCCCEEEEECCCEEEEECCC
ISDEYLGKNPRLSPWRDTHMMLEGPTVQSLQGCFLQDWYWATRQVIDVNWQVQPNWESDY
CCHHHCCCCCCCCCCCCCEEEEECCCHHHHHCHHHHHHHHHCEEEEEEEEEECCCCCCCC
TALVFPTGPADKLKACKLFFVSAINQAQTRLWIATPYFVPDDSTLTALKLAALRGVDVRI
EEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECEECCCCCCHHHHHHHHHCCCEEEE
ILPNRPDHLLVYLCSFSYYTEMKATNIKLYRYKHGFMHQKVILIDKEMAGVGTVNLDNRS
ECCCCCCEEEEEEECCHHHEEEEECEEEEEEEECCCCEEEEEEEECCCCCCEEEEECCCE
FFLNFEVMGFVANSQFVKSVEEMLQADLKAALAVDFSDYERKYLWFKLAVRISSLLKPLL
EEEEEEEEEEEECHHHHHHHHHHHHHHHHHEEEECCCHHCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIDSSVLAFFSAATVVIHGLGIAHAAHAVMDVRSSRGAIAWSISLITFPWLTIPLYWIL
CEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHH
GRTKFHGYSEAIRSVYAQHYQFVRQTYIEITKFQVVVSAKLEPLQLLAEAFIGIPFTSGN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHCCEEECCC
NAKLLINGQQTYAAMLSAIASANSYILLQSYIVVDDKAGNEFKDALIAKAKQGIRVYLIY
CCEEEEECCHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEE
DEIGSNKISRLYVKSLQKYDIQVSAFHTTRGRGNRFQLNFRNHRKILVVDGRTAFIGGLN
ECCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCEEEEEECCCCEEEEECCCEEEEECCC
ISDEYLGKNPRLSPWRDTHMMLEGPTVQSLQGCFLQDWYWATRQVIDVNWQVQPNWESDY
CCHHHCCCCCCCCCCCCCEEEEECCCHHHHHCHHHHHHHHHCEEEEEEEEEECCCCCCCC
TALVFPTGPADKLKACKLFFVSAINQAQTRLWIATPYFVPDDSTLTALKLAALRGVDVRI
EEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECEECCCCCCHHHHHHHHHCCCEEEE
ILPNRPDHLLVYLCSFSYYTEMKATNIKLYRYKHGFMHQKVILIDKEMAGVGTVNLDNRS
ECCCCCCEEEEEEECCHHHEEEEECEEEEEEEECCCCEEEEEEEECCCCCCEEEEECCCE
FFLNFEVMGFVANSQFVKSVEEMLQADLKAALAVDFSDYERKYLWFKLAVRISSLLKPLL
EEEEEEEEEEEECHHHHHHHHHHHHHHHHHEEEECCCHHCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA