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The map label for this gene is prfA [H]

Identifier: 17231677

GI number: 17231677

Start: 5034548

End: 5035648

Strand: Reverse

Name: prfA [H]

Synonym: all4185

Alternate gene names: 17231677

Gene position: 5035648-5034548 (Counterclockwise)

Preceding gene: 17231678

Following gene: 17231676

Centisome position: 78.51

GC content: 45.05

Gene sequence:

>1101_bases
ATGGCTGAATCATACCTGCTGGAGAAACTAAAATCCGTTGAACAAACCTTCAATGAATTAACTCGTCGTCTGGGAGATCC
TGACACGGCTAAAAACCCCGATGAGTATCAAAAGATTGCTAAGTCCCGTTCTTCTTTAGAAGAAGTTGTAGAGACTTATG
ATAATTGGAAAATCGCCCAAGAAGAATTAATTGGGGCGCGTCAGGTACTTAAAGAGGCAAACAGTGATCCAGATATGCAC
GAAATGGCAGCTCTGGAAGTAAAAGAATTAGAAGAAAAAATAGACTATTTAGAAACTCGTTTGAAGGTTTTGCTGCTACC
CCGTGACCCCAACGATGATAAAAACATCATGTTGGAAATTCGCGCTGGTACTGGTGGCGATGAAGCGAGTATTTGGGCTG
GTGATTTACTGCGGATGTATTCTCGCTATGCTGATACTCAAGGCTGGCGGGTAAAGTTGGTGAGTGAGTCCCTGGGTGAA
ATGGGTGGCTTCAAAGAAGTTATCCTGGAAATTCAAGGTGAGAGCGTTTACAGTAAGCTGAAATTTGAAGCAGGCGTTCA
TCGTGTGCAGCGTGTACCAGCAACCGAAGCTGGGGGAAGGGTGCATACTTCAACGGCGACAGTGGCAATTATGCCAGAAG
TTGATGAAGTAGAAATCCACATTGATCCGAAAGATATTGAAATGACTACGGCTCGTTCTGGTGGTGCTGGTGGACAAAAC
GTCAACAAGGTGGAAACTGCTGTTGACTTGATGCACAAACCGACGGGAATCCGCATTTTCTGTACAGAAGAACGCAGCCA
GTTACAAAACAAAGAACGGGCGATGCAGATTTTGCGGGCGAAGTTGTATGAGATTAAGCTGCGCGAACAACAAGAAGAAG
TGACTTCGATGAGGCGATCGCAAGTTGGAACAGGATCGCGATCGGAGAAAATTCGCACTTATAATTATAAAGATAGCCGT
GCGACTGACCACCGTTTAGGTCAAAACTTTTCGCTTAATCCAGTTTTGGAAGGCGATTTAGAGACGGTCATTCAATCTTG
TATTTCTCAAGACCAGCAGGAACGGTTGGCGGAGTTAGCAACTTCTAGCGCTGCTGGTTAA

Upstream 100 bases:

>100_bases
GTTTGGCTCTGCTGTTAACGACGACCCTGCGTCTGCTGGGTCGATTGTCATTTTTTGCCGAGCCAAGTGGTTATTTTTTA
AAGGAGCGATCGCACTCGTT

Downstream 100 bases:

>100_bases
TTATTAGTTTAATAAATTATGTTTAAACCGTCTGTGAGTAACAGGCGGTTTTTGTTTTGCTTCACGCAGAAAAGCACTTA
CGCACCCAAGTTGTCTGTGA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MAESYLLEKLKSVEQTFNELTRRLGDPDTAKNPDEYQKIAKSRSSLEEVVETYDNWKIAQEELIGARQVLKEANSDPDMH
EMAALEVKELEEKIDYLETRLKVLLLPRDPNDDKNIMLEIRAGTGGDEASIWAGDLLRMYSRYADTQGWRVKLVSESLGE
MGGFKEVILEIQGESVYSKLKFEAGVHRVQRVPATEAGGRVHTSTATVAIMPEVDEVEIHIDPKDIEMTTARSGGAGGQN
VNKVETAVDLMHKPTGIRIFCTEERSQLQNKERAMQILRAKLYEIKLREQQEEVTSMRRSQVGTGSRSEKIRTYNYKDSR
ATDHRLGQNFSLNPVLEGDLETVIQSCISQDQQERLAELATSSAAG

Sequences:

>Translated_366_residues
MAESYLLEKLKSVEQTFNELTRRLGDPDTAKNPDEYQKIAKSRSSLEEVVETYDNWKIAQEELIGARQVLKEANSDPDMH
EMAALEVKELEEKIDYLETRLKVLLLPRDPNDDKNIMLEIRAGTGGDEASIWAGDLLRMYSRYADTQGWRVKLVSESLGE
MGGFKEVILEIQGESVYSKLKFEAGVHRVQRVPATEAGGRVHTSTATVAIMPEVDEVEIHIDPKDIEMTTARSGGAGGQN
VNKVETAVDLMHKPTGIRIFCTEERSQLQNKERAMQILRAKLYEIKLREQQEEVTSMRRSQVGTGSRSEKIRTYNYKDSR
ATDHRLGQNFSLNPVLEGDLETVIQSCISQDQQERLAELATSSAAG
>Mature_365_residues
AESYLLEKLKSVEQTFNELTRRLGDPDTAKNPDEYQKIAKSRSSLEEVVETYDNWKIAQEELIGARQVLKEANSDPDMHE
MAALEVKELEEKIDYLETRLKVLLLPRDPNDDKNIMLEIRAGTGGDEASIWAGDLLRMYSRYADTQGWRVKLVSESLGEM
GGFKEVILEIQGESVYSKLKFEAGVHRVQRVPATEAGGRVHTSTATVAIMPEVDEVEIHIDPKDIEMTTARSGGAGGQNV
NKVETAVDLMHKPTGIRIFCTEERSQLQNKERAMQILRAKLYEIKLREQQEEVTSMRRSQVGTGSRSEKIRTYNYKDSRA
TDHRLGQNFSLNPVLEGDLETVIQSCISQDQQERLAELATSSAAG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=334, Percent_Identity=38.0239520958084, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI34577120, Length=289, Percent_Identity=37.7162629757785, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI166795305, Length=225, Percent_Identity=36, Blast_Score=157, Evalue=1e-38,
Organism=Escherichia coli, GI1787462, Length=357, Percent_Identity=44.2577030812325, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=38.622754491018, Blast_Score=222, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=304, Percent_Identity=36.1842105263158, Blast_Score=187, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=334, Percent_Identity=40.1197604790419, Blast_Score=211, Evalue=1e-55,
Organism=Drosophila melanogaster, GI19921226, Length=340, Percent_Identity=36.1764705882353, Blast_Score=211, Evalue=6e-55,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41236; Mature: 41105

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESYLLEKLKSVEQTFNELTRRLGDPDTAKNPDEYQKIAKSRSSLEEVVETYDNWKIAQ
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
EELIGARQVLKEANSDPDMHEMAALEVKELEEKIDYLETRLKVLLLPRDPNDDKNIMLEI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEE
RAGTGGDEASIWAGDLLRMYSRYADTQGWRVKLVSESLGEMGGFKEVILEIQGESVYSKL
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCHHHHHHHHCCHHHHHHH
KFEAGVHRVQRVPATEAGGRVHTSTATVAIMPEVDEVEIHIDPKDIEMTTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCEEEEEECCCCCEEEECCCCCCCCCC
VNKVETAVDLMHKPTGIRIFCTEERSQLQNKERAMQILRAKLYEIKLREQQEEVTSMRRS
HHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVGTGSRSEKIRTYNYKDSRATDHRLGQNFSLNPVLEGDLETVIQSCISQDQQERLAELA
HCCCCCCCCCEEECCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHH
TSSAAG
HHCCCC
>Mature Secondary Structure 
AESYLLEKLKSVEQTFNELTRRLGDPDTAKNPDEYQKIAKSRSSLEEVVETYDNWKIAQ
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
EELIGARQVLKEANSDPDMHEMAALEVKELEEKIDYLETRLKVLLLPRDPNDDKNIMLEI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEE
RAGTGGDEASIWAGDLLRMYSRYADTQGWRVKLVSESLGEMGGFKEVILEIQGESVYSKL
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCHHHHHHHHCCHHHHHHH
KFEAGVHRVQRVPATEAGGRVHTSTATVAIMPEVDEVEIHIDPKDIEMTTARSGGAGGQN
HHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCEEEEEECCCCCEEEECCCCCCCCCC
VNKVETAVDLMHKPTGIRIFCTEERSQLQNKERAMQILRAKLYEIKLREQQEEVTSMRRS
HHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVGTGSRSEKIRTYNYKDSRATDHRLGQNFSLNPVLEGDLETVIQSCISQDQQERLAELA
HCCCCCCCCCEEECCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHH
TSSAAG
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA