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The map label for this gene is cofH [H]

Identifier: 17231621

GI number: 17231621

Start: 4976929

End: 4978104

Strand: Direct

Name: cofH [H]

Synonym: alr4129

Alternate gene names: 17231621

Gene position: 4976929-4978104 (Clockwise)

Preceding gene: 161353738

Following gene: 17231624

Centisome position: 77.6

GC content: 45.32

Gene sequence:

>1176_bases
GTGAATTATAAAACTGTTGATGTAATTCTTGAGCGCGCCTTGCTAGGTGATGATATATCACCACAAGAGGGAGTAGTCTT
ACTAACGCAAACGGACTCAGGCGCGATCGCCTCTATTCGCGCTACAGCTGACAAACTCCGCCAACAGCAAGCCGGGGATA
CAGTTACTTACGTAATTAACCGGAATATTAATTTTACTAACATTTGTGAGCAGCACTGTAGTTTTTGTGCTTTTCGGCGA
GATGATGGGGATGCGGACGCTTATTGGTTAGATTGGGCAGGTATTCGGGAAAAATCCCACGACGCGGTGCAGAGGGGAGC
GACGGAAATCTGTATGCAGGGAGGTTTGCACCCACAAGCCCAGATTGATGGTAAATCTCTGCCTTATTACCTCAAACTAG
TAGAAACGATTAAGCAAGAATATCCCCAAATTCATCTACACGCTTTTTCACCCCAAGAGGTGCAGTTTATCGCTAGAGTG
GACGGACTGGAATATGTAGACGTAATCTCTGCTTTGCAAAATGCTGGCGTTAACTCCCTACCAGGAACAGCAGCCGAAGT
TTTAGATGATGAAGTGCGGCGCATACTCTGTCCTGAAAAGATTAATACTGCTACTTGGTTGGAAATTATTGGTACAGCCC
ACAAAGTAGGCTTACATACTACCAGCACTATATTATCAGGGCATATTGAAACACCAGAACAAAAAATTGGGCATTTAGAA
AAATTGCGATCGCTCCAACAAACAGCCATCAATCATAAATACCCTGCCAGAATTACCGAGTTTATTGTCTTACCTTTTGT
CGGTCAAGAAGCACCTAAATCGCTACGTCGCCGTGTAGGACGTGACCAACCAATTTTAGCCGATGCCTTATTACTGGGTG
CAGTGGCAAGAATTTACTTAGGTAATTGGATACCCAACCATCAACCAAGTTGGGTCAAGTTGGGGCTAGCTGGGGCGACA
GAAGCCCTAACATGGGGCTGTAACGATATAGGCGGTACATTGATGGAAGAACACATCACCACAATGGCTGGTGCTGTAGG
CGGTACGTGCATGGAAGTCGCAACCTTGCAAAATGCGATCGCTTCCATAGGCAGACCTTACCAACAACGAGATACACTTT
ATCACAAGGTAGATGCAGCCAAAAAACTGGCTAATACGGTAATGAGTAATGGGTAA

Upstream 100 bases:

>100_bases
AAAGTGTGAAAGTTATATAATTTTTTGGTGTGATTAACGACAAAGTAAGTATTTGTATGCTCGTAAGTACAAAATTTTGT
AGAGATATTTTTAATTTACT

Downstream 100 bases:

>100_bases
TTGGTGAAAATTTATTCTCAATTGCCAATCACCTATTACCTATTACCATCTAAAAGTTAAAGTTCACGCTGAGGAATTTC
TTCCCGAAACTCGATAAACA

Product: FO synthase subunit 2

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MNYKTVDVILERALLGDDISPQEGVVLLTQTDSGAIASIRATADKLRQQQAGDTVTYVINRNINFTNICEQHCSFCAFRR
DDGDADAYWLDWAGIREKSHDAVQRGATEICMQGGLHPQAQIDGKSLPYYLKLVETIKQEYPQIHLHAFSPQEVQFIARV
DGLEYVDVISALQNAGVNSLPGTAAEVLDDEVRRILCPEKINTATWLEIIGTAHKVGLHTTSTILSGHIETPEQKIGHLE
KLRSLQQTAINHKYPARITEFIVLPFVGQEAPKSLRRRVGRDQPILADALLLGAVARIYLGNWIPNHQPSWVKLGLAGAT
EALTWGCNDIGGTLMEEHITTMAGAVGGTCMEVATLQNAIASIGRPYQQRDTLYHKVDAAKKLANTVMSNG

Sequences:

>Translated_391_residues
MNYKTVDVILERALLGDDISPQEGVVLLTQTDSGAIASIRATADKLRQQQAGDTVTYVINRNINFTNICEQHCSFCAFRR
DDGDADAYWLDWAGIREKSHDAVQRGATEICMQGGLHPQAQIDGKSLPYYLKLVETIKQEYPQIHLHAFSPQEVQFIARV
DGLEYVDVISALQNAGVNSLPGTAAEVLDDEVRRILCPEKINTATWLEIIGTAHKVGLHTTSTILSGHIETPEQKIGHLE
KLRSLQQTAINHKYPARITEFIVLPFVGQEAPKSLRRRVGRDQPILADALLLGAVARIYLGNWIPNHQPSWVKLGLAGAT
EALTWGCNDIGGTLMEEHITTMAGAVGGTCMEVATLQNAIASIGRPYQQRDTLYHKVDAAKKLANTVMSNG
>Mature_391_residues
MNYKTVDVILERALLGDDISPQEGVVLLTQTDSGAIASIRATADKLRQQQAGDTVTYVINRNINFTNICEQHCSFCAFRR
DDGDADAYWLDWAGIREKSHDAVQRGATEICMQGGLHPQAQIDGKSLPYYLKLVETIKQEYPQIHLHAFSPQEVQFIARV
DGLEYVDVISALQNAGVNSLPGTAAEVLDDEVRRILCPEKINTATWLEIIGTAHKVGLHTTSTILSGHIETPEQKIGHLE
KLRSLQQTAINHKYPARITEFIVLPFVGQEAPKSLRRRVGRDQPILADALLLGAVARIYLGNWIPNHQPSWVKLGLAGAT
EALTWGCNDIGGTLMEEHITTMAGAVGGTCMEVATLQNAIASIGRPYQQRDTLYHKVDAAKKLANTVMSNG

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42981; Mature: 42981

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKTVDVILERALLGDDISPQEGVVLLTQTDSGAIASIRATADKLRQQQAGDTVTYVIN
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEE
RNINFTNICEQHCSFCAFRRDDGDADAYWLDWAGIREKSHDAVQRGATEICMQGGLHPQA
CCCCHHHHHHHHHHHEEEECCCCCCCEEEEECHHCCCCHHHHHHHHHHHHHHHCCCCCCC
QIDGKSLPYYLKLVETIKQEYPQIHLHAFSPQEVQFIARVDGLEYVDVISALQNAGVNSL
CCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
PGTAAEVLDDEVRRILCPEKINTATWLEIIGTAHKVGLHTTSTILSGHIETPEQKIGHLE
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHH
KLRSLQQTAINHKYPARITEFIVLPFVGQEAPKSLRRRVGRDQPILADALLLGAVARIYL
HHHHHHHHHHCCCCCHHHHHHHEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GNWIPNHQPSWVKLGLAGATEALTWGCNDIGGTLMEEHITTMAGAVGGTCMEVATLQNAI
CCCCCCCCCCEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ASIGRPYQQRDTLYHKVDAAKKLANTVMSNG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNYKTVDVILERALLGDDISPQEGVVLLTQTDSGAIASIRATADKLRQQQAGDTVTYVIN
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEE
RNINFTNICEQHCSFCAFRRDDGDADAYWLDWAGIREKSHDAVQRGATEICMQGGLHPQA
CCCCHHHHHHHHHHHEEEECCCCCCCEEEEECHHCCCCHHHHHHHHHHHHHHHCCCCCCC
QIDGKSLPYYLKLVETIKQEYPQIHLHAFSPQEVQFIARVDGLEYVDVISALQNAGVNSL
CCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
PGTAAEVLDDEVRRILCPEKINTATWLEIIGTAHKVGLHTTSTILSGHIETPEQKIGHLE
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHH
KLRSLQQTAINHKYPARITEFIVLPFVGQEAPKSLRRRVGRDQPILADALLLGAVARIYL
HHHHHHHHHHCCCCCHHHHHHHEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GNWIPNHQPSWVKLGLAGATEALTWGCNDIGGTLMEEHITTMAGAVGGTCMEVATLQNAI
CCCCCCCCCCEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ASIGRPYQQRDTLYHKVDAAKKLANTVMSNG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA