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The map label for this gene is metK [H]

Identifier: 161353738

GI number: 161353738

Start: 4967988

End: 4969250

Strand: Direct

Name: metK [H]

Synonym: alr4124

Alternate gene names: 161353738

Gene position: 4967988-4969250 (Clockwise)

Preceding gene: 17231615

Following gene: 17231621

Centisome position: 77.46

GC content: 46.79

Gene sequence:

>1263_bases
TTGTCTCGACGTTATTTATTTACCTCCGAGTCAGTCACCGAAGGCCATCCAGATAAAATCTGCGATCAAATTTCTGATAC
GATTTTAGACACTTTGCTGGCACAAGACCCGACTAGTCGGGTGGCAGCTGAGGTTGTGGTAAATACTGGCTTGGTGTTAA
TCACCGGTGAAATCACCACCAAGGCTAACGTTAATTATGCCAATATTGCCCGCCAAAAAATTGCGGAAATTGGCTACACC
AATGCTGATAATGGCTTTTCTGCTAACAGCACTAGTGTAATTGTGGCTTTAGATGAACAATCACCAGATATTGCTCAAGG
TGTCAACACTGCTCAAGAAACCCGCGAACAGGATAGTGAAGAACTATTCGATAAAATCGGCGCTGGTGATCAAGGTATCA
TGTTCGGTTTTGCTTGTAACGAAACTCCAGAATTGATGCCTTTGCCTATTTGCTTGGCTCACCGCATTGCTCGCCGACTA
GCAGCAGTCCGTAAGACTGGGGAGTTGTCCTACTTACGTCCTGATGGTAAAACTCAAGTTACCGTCGTTTATGAAGATGG
CCGCCCAGTTGGCATTGATACAGTGCTGATTTCCACCCAGCATACAGCCACCATCGGGGATATTACTGATGAAGCGGCAG
TACAAGCCAAAATTAAACAAGACCTGTGGACTGCTGTAGTGGAACCAGTGTTTGGTGACTTGGAAATTAAACCAGATCAA
GAGACACGCTTTTTAGTTAACCCCACTGGCAAGTTTGTCATTGGTGGCCCGCAAGGCGACTCTGGTTTGACAGGACGGAA
AATTATCGTTGATACCTATGGCGGTTACTCCCGACATGGTGGTGGCGCTTTCTCTGGCAAAGACCCCACAAAGGTAGACC
GTTCTGCGGCTTATGCGGCTCGTTATGTAGCTAAAAATGTTGTAGCTGCCGGGTTAGCAGAAAAATGTGAAGTCCAGTTG
AGCTATGCGATTGGTGTAGCGCGACCGGTGAGTATTTTTCTAGATACCTTCGGGACTGGCAAGGTAGATGATGAAATTCT
GTTGGAACTAGTTAAAGATAACTTTGAACTACGTCCAGCAGGGATTATCCACAGCTTCAACTTACGCAACTTACCAAGCG
AACGAGGCGGACGTTTTTATCAGGACGTCGCGGCTTACGGTCACTTGGGACGGAACGATCTAGATTTACCCTGGGAACGT
ACCGATAAGGCGGATTTCTTGAAGCAAGCGGTTACTCATTCATTGTCAGCTGCGATCGCCTAG

Upstream 100 bases:

>100_bases
CTGGATGTGTCAAGATTGTTATGATTTCAGTAATTAGTAGCTTGACTAAATATCTCAAATGTCTAGTCAGTGAAAATACT
ATCATTAGGAGGAATACGAA

Downstream 100 bases:

>100_bases
ATACTCTCTAATCCAGATACCCGACTTTTCTCAAAAGTCGGGTATCTTTGTTCACCGAGACTTTTCCCGACAGTGATAAA
TTTTGTATTCATAGCCGTCT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSRRYLFTSESVTEGHPDKICDQISDTILDTLLAQDPTSRVAAEVVVNTGLVLITGEITTKANVNYANIARQKIAEIGYT
NADNGFSANSTSVIVALDEQSPDIAQGVNTAQETREQDSEELFDKIGAGDQGIMFGFACNETPELMPLPICLAHRIARRL
AAVRKTGELSYLRPDGKTQVTVVYEDGRPVGIDTVLISTQHTATIGDITDEAAVQAKIKQDLWTAVVEPVFGDLEIKPDQ
ETRFLVNPTGKFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKNVVAAGLAEKCEVQL
SYAIGVARPVSIFLDTFGTGKVDDEILLELVKDNFELRPAGIIHSFNLRNLPSERGGRFYQDVAAYGHLGRNDLDLPWER
TDKADFLKQAVTHSLSAAIA

Sequences:

>Translated_420_residues
MSRRYLFTSESVTEGHPDKICDQISDTILDTLLAQDPTSRVAAEVVVNTGLVLITGEITTKANVNYANIARQKIAEIGYT
NADNGFSANSTSVIVALDEQSPDIAQGVNTAQETREQDSEELFDKIGAGDQGIMFGFACNETPELMPLPICLAHRIARRL
AAVRKTGELSYLRPDGKTQVTVVYEDGRPVGIDTVLISTQHTATIGDITDEAAVQAKIKQDLWTAVVEPVFGDLEIKPDQ
ETRFLVNPTGKFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKNVVAAGLAEKCEVQL
SYAIGVARPVSIFLDTFGTGKVDDEILLELVKDNFELRPAGIIHSFNLRNLPSERGGRFYQDVAAYGHLGRNDLDLPWER
TDKADFLKQAVTHSLSAAIA
>Mature_419_residues
SRRYLFTSESVTEGHPDKICDQISDTILDTLLAQDPTSRVAAEVVVNTGLVLITGEITTKANVNYANIARQKIAEIGYTN
ADNGFSANSTSVIVALDEQSPDIAQGVNTAQETREQDSEELFDKIGAGDQGIMFGFACNETPELMPLPICLAHRIARRLA
AVRKTGELSYLRPDGKTQVTVVYEDGRPVGIDTVLISTQHTATIGDITDEAAVQAKIKQDLWTAVVEPVFGDLEIKPDQE
TRFLVNPTGKFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKNVVAAGLAEKCEVQLS
YAIGVARPVSIFLDTFGTGKVDDEILLELVKDNFELRPAGIIHSFNLRNLPSERGGRFYQDVAAYGHLGRNDLDLPWERT
DKADFLKQAVTHSLSAAIA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=406, Percent_Identity=50.2463054187192, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI4557737, Length=406, Percent_Identity=50.9852216748768, Blast_Score=364, Evalue=1e-101,
Organism=Escherichia coli, GI1789311, Length=407, Percent_Identity=55.2825552825553, Blast_Score=444, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17538498, Length=402, Percent_Identity=50.2487562189055, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25145633, Length=409, Percent_Identity=50.1222493887531, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17538494, Length=402, Percent_Identity=49.7512437810945, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17551082, Length=404, Percent_Identity=48.5148514851485, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32565905, Length=402, Percent_Identity=45.5223880597015, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI32565911, Length=354, Percent_Identity=48.0225988700565, Blast_Score=304, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI25145635, Length=358, Percent_Identity=47.7653631284916, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI32565903, Length=327, Percent_Identity=48.3180428134557, Blast_Score=277, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=54.7619047619048, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320710, Length=409, Percent_Identity=49.6332518337408, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6323209, Length=410, Percent_Identity=49.7560975609756, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45552159, Length=399, Percent_Identity=47.8696741854637, Blast_Score=338, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24580529, Length=399, Percent_Identity=47.8696741854637, Blast_Score=338, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24580537, Length=399, Percent_Identity=47.8696741854637, Blast_Score=338, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24580533, Length=399, Percent_Identity=47.8696741854637, Blast_Score=338, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24580535, Length=399, Percent_Identity=47.8696741854637, Blast_Score=338, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24580531, Length=399, Percent_Identity=47.8696741854637, Blast_Score=338, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24580527, Length=399, Percent_Identity=47.6190476190476, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24580525, Length=399, Percent_Identity=47.6190476190476, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24580541, Length=386, Percent_Identity=46.3730569948187, Blast_Score=314, Evalue=9e-86,
Organism=Drosophila melanogaster, GI24580539, Length=162, Percent_Identity=43.2098765432099, Blast_Score=135, Evalue=8e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 45436; Mature: 45304

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRYLFTSESVTEGHPDKICDQISDTILDTLLAQDPTSRVAAEVVVNTGLVLITGEITT
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEECEEE
KANVNYANIARQKIAEIGYTNADNGFSANSTSVIVALDEQSPDIAQGVNTAQETREQDSE
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCHHHHHHHHHHH
ELFDKIGAGDQGIMFGFACNETPELMPLPICLAHRIARRLAAVRKTGELSYLRPDGKTQV
HHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEE
TVVYEDGRPVGIDTVLISTQHTATIGDITDEAAVQAKIKQDLWTAVVEPVFGDLEIKPDQ
EEEEECCCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
ETRFLVNPTGKFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAA
CCEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
RYVAKNVVAAGLAEKCEVQLSYAIGVARPVSIFLDTFGTGKVDDEILLELVKDNFELRPA
HHHHHHHHHHCCHHHHEEEEEEECCCCCHHHHHEECCCCCCCCHHHHHHHHHCCCCCCCC
GIIHSFNLRNLPSERGGRFYQDVAAYGHLGRNDLDLPWERTDKADFLKQAVTHSLSAAIA
CEEEECCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRRYLFTSESVTEGHPDKICDQISDTILDTLLAQDPTSRVAAEVVVNTGLVLITGEITT
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEECEEE
KANVNYANIARQKIAEIGYTNADNGFSANSTSVIVALDEQSPDIAQGVNTAQETREQDSE
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCHHHHHHHHHHH
ELFDKIGAGDQGIMFGFACNETPELMPLPICLAHRIARRLAAVRKTGELSYLRPDGKTQV
HHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEE
TVVYEDGRPVGIDTVLISTQHTATIGDITDEAAVQAKIKQDLWTAVVEPVFGDLEIKPDQ
EEEEECCCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
ETRFLVNPTGKFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAA
CCEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
RYVAKNVVAAGLAEKCEVQLSYAIGVARPVSIFLDTFGTGKVDDEILLELVKDNFELRPA
HHHHHHHHHHCCHHHHEEEEEEECCCCCHHHHHEECCCCCCCCHHHHHHHHHCCCCCCCC
GIIHSFNLRNLPSERGGRFYQDVAAYGHLGRNDLDLPWERTDKADFLKQAVTHSLSAAIA
CEEEECCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA