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The map label for this gene is cobA [H]

Identifier: 17230942

GI number: 17230942

Start: 4158588

End: 4160141

Strand: Direct

Name: cobA [H]

Synonym: alr3450

Alternate gene names: 17230942

Gene position: 4158588-4160141 (Clockwise)

Preceding gene: 17230941

Following gene: 17230943

Centisome position: 64.84

GC content: 43.05

Gene sequence:

>1554_bases
ATGACTCAACAAAATGGCAAAGTTTACCTTGTAGGTGCGGGGCCGGGAGATGTGGCATACCTCACAGTGAAAGCTTATAA
TCTACTGGCTACGGCTCAAGTGTTGGTTTATGATGCCCTAGTGGATGAGCAATTGTTGCAGTGTGTATCATCTGATTGTC
TGAAACTAGATGTAGGGAAGCGTGGCGGTAAACCCAGCACACCGCAAACGGAAATCAATAATTTATTAGTTAAGTTCTGC
CAAGAAGGTGCATTAGTCGTTAGGTTGAAATCAGGTGACCCGTTTATTTTTGGTCGGAGTACCTCAGAAATTGAGGCTTT
GAAAGCAGCAGGCTGTGAATTTGAAGTAGTACCAGGAATTTCCTCAGCTATAGCAGCACCTTTATTAGCAGGAATCCCGC
TTACAGACCCGGTGATGAGTCGTTGCTTTGCAGTGCTGACAGCCCATGAACCAGAAGTTTTAGACTGGGAGGCGCTATCA
AGGTTAGACACCCTAGTCATATTAATGGGTGGGAAAAACCTCGCAGATATTGTACATGAACTTCTACGGAGAGAGCGATC
GCATCTCACACCTATAGCTATTATTCGCTGGGCAGGAACCCCCAGTCAAGAAATTTGGACTGGTCAACTGGGGGATATTC
TCCAACAAACAGCAGGTTTATCTCTCTCCCCAGTCGTTATCGTCATTGGTGAAGTCGTCAGGCTACGCAAGTACCTACAA
CCTGAGAAAATATTTCCAGAGAACTCAACTACACCTATGTCCAGCAACCAACCTCTCACTGGAAAAACCATCCTCGTCAC
CCGTTCATCTGGTCAATCGAGTCAATTTAGCGATCGCCTAACTACACTTGGCGCTACAGTAATTGAAATGCCAGCGTTAG
AAATAGGCCCCCCTTCTAGTTGGAAAGAGTTAGATCAGGCGATCGCTAATTTATCTCACTTTGACTGGTTAATTCTCACT
TCCACCAACGGCGTAGATTATTTCTTTGAAAGGCTATATATCCAAGGTAAAGATACTCGTGCTTTAGCTGGGTTAAAAAT
CGCTGTCGTTGGGGAAAAAACCTCCCAAAGCCTCAAACAAAGAGGAATCCAAGCAGATTTTATTCCCCCTAACTTTGTGG
CTGATTCTTTAGTAGAGCATTTTCCCGAATCACTACTAAATAAAAAGATTTTATTCCCCAGAGTGGAAAGCGGCGGTAGG
GAAATTTTAGTCAAAGAATTATCCGCAAAAGGTGCAGATGTCATTGAAGTAGCTGCTTATCAATCTTGTTGTCCCACTAG
TATTCCCCTAGCAGCAGAACTAGCCTTAAAAAATCGCACAATACATATCATTACCTTTGCCAGTTCTAAAACCGTGCAGT
TTTTCTGTCAACTTGCAGACAAAATATTTCCTCACAATATTCCCAATGCTTTAGAAGGAATATGTATTGCTTCCATCGGA
CCCCAAACTTCAAAAACTTGTCATACCCTATTAGGTAGAGTCGATATAGAAGCTGAAGAATACACCTTAGATGGATTAAC
CCAAGCAATCATCCAATGGTCATTAAAACCCTAA

Upstream 100 bases:

>100_bases
AAAGGGTTGCTAAATTATTAGAGCAAATGAATACTAAGACAATCTGAGTAATATGAGTGATGTTTACGACGATGCTACCC
CTACGCACTAATCTAAAATC

Downstream 100 bases:

>100_bases
AACCCTCATCAATGAACCAATCAAAATAACTTCCCTAAATCCCAGCCAATCAGCTTCAAAAAACCTCCGCGTCACTCTGC
TCTTACCTCCCATCCCTCTG

Product: uroporphyrinogen III methylase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MTQQNGKVYLVGAGPGDVAYLTVKAYNLLATAQVLVYDALVDEQLLQCVSSDCLKLDVGKRGGKPSTPQTEINNLLVKFC
QEGALVVRLKSGDPFIFGRSTSEIEALKAAGCEFEVVPGISSAIAAPLLAGIPLTDPVMSRCFAVLTAHEPEVLDWEALS
RLDTLVILMGGKNLADIVHELLRRERSHLTPIAIIRWAGTPSQEIWTGQLGDILQQTAGLSLSPVVIVIGEVVRLRKYLQ
PEKIFPENSTTPMSSNQPLTGKTILVTRSSGQSSQFSDRLTTLGATVIEMPALEIGPPSSWKELDQAIANLSHFDWLILT
STNGVDYFFERLYIQGKDTRALAGLKIAVVGEKTSQSLKQRGIQADFIPPNFVADSLVEHFPESLLNKKILFPRVESGGR
EILVKELSAKGADVIEVAAYQSCCPTSIPLAAELALKNRTIHIITFASSKTVQFFCQLADKIFPHNIPNALEGICIASIG
PQTSKTCHTLLGRVDIEAEEYTLDGLTQAIIQWSLKP

Sequences:

>Translated_517_residues
MTQQNGKVYLVGAGPGDVAYLTVKAYNLLATAQVLVYDALVDEQLLQCVSSDCLKLDVGKRGGKPSTPQTEINNLLVKFC
QEGALVVRLKSGDPFIFGRSTSEIEALKAAGCEFEVVPGISSAIAAPLLAGIPLTDPVMSRCFAVLTAHEPEVLDWEALS
RLDTLVILMGGKNLADIVHELLRRERSHLTPIAIIRWAGTPSQEIWTGQLGDILQQTAGLSLSPVVIVIGEVVRLRKYLQ
PEKIFPENSTTPMSSNQPLTGKTILVTRSSGQSSQFSDRLTTLGATVIEMPALEIGPPSSWKELDQAIANLSHFDWLILT
STNGVDYFFERLYIQGKDTRALAGLKIAVVGEKTSQSLKQRGIQADFIPPNFVADSLVEHFPESLLNKKILFPRVESGGR
EILVKELSAKGADVIEVAAYQSCCPTSIPLAAELALKNRTIHIITFASSKTVQFFCQLADKIFPHNIPNALEGICIASIG
PQTSKTCHTLLGRVDIEAEEYTLDGLTQAIIQWSLKP
>Mature_516_residues
TQQNGKVYLVGAGPGDVAYLTVKAYNLLATAQVLVYDALVDEQLLQCVSSDCLKLDVGKRGGKPSTPQTEINNLLVKFCQ
EGALVVRLKSGDPFIFGRSTSEIEALKAAGCEFEVVPGISSAIAAPLLAGIPLTDPVMSRCFAVLTAHEPEVLDWEALSR
LDTLVILMGGKNLADIVHELLRRERSHLTPIAIIRWAGTPSQEIWTGQLGDILQQTAGLSLSPVVIVIGEVVRLRKYLQP
EKIFPENSTTPMSSNQPLTGKTILVTRSSGQSSQFSDRLTTLGATVIEMPALEIGPPSSWKELDQAIANLSHFDWLILTS
TNGVDYFFERLYIQGKDTRALAGLKIAVVGEKTSQSLKQRGIQADFIPPNFVADSLVEHFPESLLNKKILFPRVESGGRE
ILVKELSAKGADVIEVAAYQSCCPTSIPLAAELALKNRTIHIITFASSKTVQFFCQLADKIFPHNIPNALEGICIASIGP
QTSKTCHTLLGRVDIEAEEYTLDGLTQAIIQWSLKP

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=243, Percent_Identity=41.1522633744856, Blast_Score=161, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6322922, Length=239, Percent_Identity=30.1255230125523, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 56171; Mature: 56039

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQNGKVYLVGAGPGDVAYLTVKAYNLLATAQVLVYDALVDEQLLQCVSSDCLKLDVGK
CCCCCCEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
RGGKPSTPQTEINNLLVKFCQEGALVVRLKSGDPFIFGRSTSEIEALKAAGCEFEVVPGI
CCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEECCCCHHHHHHHHCCCCEEECCCC
SSAIAAPLLAGIPLTDPVMSRCFAVLTAHEPEVLDWEALSRLDTLVILMGGKNLADIVHE
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCEEEEEECCCCHHHHHHH
LLRRERSHLTPIAIIRWAGTPSQEIWTGQLGDILQQTAGLSLSPVVIVIGEVVRLRKYLQ
HHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
PEKIFPENSTTPMSSNQPLTGKTILVTRSSGQSSQFSDRLTTLGATVIEMPALEIGPPSS
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
WKELDQAIANLSHFDWLILTSTNGVDYFFERLYIQGKDTRALAGLKIAVVGEKTSQSLKQ
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHEECCCCCEEECCEEEEEECCHHHHHHHH
RGIQADFIPPNFVADSLVEHFPESLLNKKILFPRVESGGREILVKELSAKGADVIEVAAY
CCCEECCCCCHHHHHHHHHHHHHHHHCCCEECEECCCCCCEEHHHHHCCCCCCCEEHHHH
QSCCPTSIPLAAELALKNRTIHIITFASSKTVQFFCQLADKIFPHNIPNALEGICIASIG
HCCCCCCCCHHHHHEECCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHCCEEEECCC
PQTSKTCHTLLGRVDIEAEEYTLDGLTQAIIQWSLKP
CCCHHHHHHHHHCEECCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TQQNGKVYLVGAGPGDVAYLTVKAYNLLATAQVLVYDALVDEQLLQCVSSDCLKLDVGK
CCCCCEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
RGGKPSTPQTEINNLLVKFCQEGALVVRLKSGDPFIFGRSTSEIEALKAAGCEFEVVPGI
CCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEECCCCHHHHHHHHCCCCEEECCCC
SSAIAAPLLAGIPLTDPVMSRCFAVLTAHEPEVLDWEALSRLDTLVILMGGKNLADIVHE
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCEEEEEECCCCHHHHHHH
LLRRERSHLTPIAIIRWAGTPSQEIWTGQLGDILQQTAGLSLSPVVIVIGEVVRLRKYLQ
HHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
PEKIFPENSTTPMSSNQPLTGKTILVTRSSGQSSQFSDRLTTLGATVIEMPALEIGPPSS
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
WKELDQAIANLSHFDWLILTSTNGVDYFFERLYIQGKDTRALAGLKIAVVGEKTSQSLKQ
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHEECCCCCEEECCEEEEEECCHHHHHHHH
RGIQADFIPPNFVADSLVEHFPESLLNKKILFPRVESGGREILVKELSAKGADVIEVAAY
CCCEECCCCCHHHHHHHHHHHHHHHHCCCEECEECCCCCCEEHHHHHCCCCCCCEEHHHH
QSCCPTSIPLAAELALKNRTIHIITFASSKTVQFFCQLADKIFPHNIPNALEGICIASIG
HCCCCCCCCHHHHHEECCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHCCEEEECCC
PQTSKTCHTLLGRVDIEAEEYTLDGLTQAIIQWSLKP
CCCHHHHHHHHHCEECCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8123787 [H]