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The map label for this gene is ppsA [H]

Identifier: 17230889

GI number: 17230889

Start: 4105145

End: 4107472

Strand: Direct

Name: ppsA [H]

Synonym: alr3397

Alternate gene names: 17230889

Gene position: 4105145-4107472 (Clockwise)

Preceding gene: 17230887

Following gene: 17230891

Centisome position: 64.01

GC content: 44.42

Gene sequence:

>2328_bases
GTGGATAAACTCTACTGGCTTGACCAAATTAAATTACAAGACCGCGCTAAAGTAGGTGACAAAGCGTTCTATTTAAGTAG
AATCAGGCAGCGTGGCTACCCGGTTGTTCCTGGTTTTGTAGTGTCTGAAGAAGTTTTGCGATTATTTCTAGAAACTCTCA
ACAGTTCAGAATCATTGGTCGCTAACTTACCTAATTCTTCCTTACACTTGGATGTAGCCAACTGGCGACAACTCCAGCAG
GTGGCTGGGCGTTTACGTCAGGAAATTATTCATGCAACTGTACCATCACAGTGGGTGAATACAATTCTTACGGCCGCTAG
GGAATGGCAAACAAACTTTTTAATTTTTCGCCCTAGTTTAACACTAGCAACAAAAACCCACAGGCTAGGAAACACCTCTG
GATTATTGGATGCTGCTTTCTGTTACTGCAATGAAGACGCGATCGCTCACACACTCAAGCATACCTGGAGTCAACTTTTT
CGCGCCAGAAGCTTGCTATATTGGCAGCGCATAGGAGTTAACCTCCAAGAAGTTAATCTAGCAATCTTAGTACAACCAGT
ACAAAATGCGATCGCCAGTGGTTTATTAATCGCTAACTCTTCAGGCTGTAAAATTCAAGCTACATGGGGATTAGGATTAG
CCTTGTCTCAGGGCGAGATTATACCAGATACATACCAAGTTCAGCAGGAAACAGGTATTGTCTTAGAAAGACATCTGGGA
AATAAAATGTTTGCTTATCGTCTAGATAGTTCAGAATTGAATGATGCCAGACTATCTAGAAAAGAGGTATTCACATTAAA
TCACCCTTGTATAGGCGCGTATTCAGTACAAGAGTCACACCAGCAACAATATGCTTTAGCCGAAGAATATCTACAACAAG
TAATTACCTTAGGACATCAACTGGAAAGCGAACTAGGTAACAGTTTTACTATTGAATGGACAATTGCAGAGGAAAACTCC
ACCCCCTGTCTCTACATTACGCAAGTGAGTACTCCCCAATCTGCAATTCCCAATTTACGTTTCATCAAGGGTGCTGGGGC
GGCTAGAGGACGCGTCACCGCTAATGCTTATGTTGTCAATGACTCCCAACGGAAACCAGAACAACTGCCCAAAGGCGTAA
TTTTAGTGGTACCAGCAATTACACCTGATTGGTTGTCGTTAGTAGAACAAGCCGTTGGTATTGTCACAGTCCAAGGAGGT
GTAACTAGTCATGCAGCGATTTTGGCCAGGGAATTGGGTATTCCAGCCGTTGTGAATGTAGCAGATGCCACCTTACTAAT
TCCCAATGGTGAACGATTGCTAATAGATGGCGATCGCGGAGAGGTATATTTACTAAAAGCAGATGAAGCCACTACGGTAG
AAAATCCACACCAGCAACCTCTGACTCCACCATTTCACCCTGTTGTTAGCAGGCAAATGCCAATGATTGCTACCCAACTG
CTGGTTAACCTCAGTCAACCTAGTCTAATTGAACAAGTGCAGAACTTGCCTGTAGATGGGGTGGGATTATTGCGTTCAGA
GTTGATGTTAGTACCTTTACTCAAAGGTCAGCATCCGAATTTTTGGCTTTTGGATGGGCGGAAATCAGAATTATTAGAGC
AGTGGTCTGAGCATATTATCCAGTTTGCCCGCGCCTTTGCCCCCCGACCGGTTTTTTATCGCTCTTTAGATTGGCGATCG
CACGAGTTATCAGCAAATAATTCCCAATCTGCGCCTCAGTCAATTTTGGGCGATCGCGGTACATTTAGTTATGTCCGCAA
TTCTGATGTTTTTGAATTAGAACTTACAGCGATCGCTAATGCCCAAAAAGCTGGTTATGATAACATTCACTTATTATTGC
CCTTCGTTCGCAGTGTAGGCGAATTTACCTTCTGTCGCCAAAAAGCAGAACAGTTCGGACTTACCCAAATTCCCCAGTTT
CAATTATGGATGATGGCGGAAGTCCCCAGCGTCTTATTTTTACTACCTGAATACGTCAAAGCCGGGGTAGCGGGTATATC
CATCGGCACAAACGACTTGACACAATTGCTATTAGGCGCAGACAGGGAACAAAGCGACATCACCAAAGTTTTAAATGAAC
GCCACCCGGCTGTCATGGCTGCTATTGCCCAACTAATCCAAATGTCCCAAACTGCCGGCATACCTTGTTCTATCTGCGGT
CAAGCACCAGCAATATATCCAGAAATCATCGATCAATTGGTGCAATGGGGTATCACATCGATTTCCGTGGAACCAGAAGC
AGTAGAAAGGACATATCAGGCGATCGCTCGTGCTGAACACCGCTTACTATTACAAGCAGCACGCCGACAGGTGGGAGAGC
GTGGTTAA

Upstream 100 bases:

>100_bases
AAAAATACTTATGAAGGAGGCAATTAACAAAGGGGATAGTGCAACTACTAACCACAAATATAAAAACTTCTCCTGGCAAA
CTGACAATCAAAAAAATTGA

Downstream 100 bases:

>100_bases
AATGTGTAGACTTTTAATGTTTTCTCAAACCCTGGTTTTAGGGTGTAAGTTTCGATAATTTCAAAATTCTGTCACCACTA
AATCGCCCAAGACCAAGAAA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 775; Mature: 775

Protein sequence:

>775_residues
MDKLYWLDQIKLQDRAKVGDKAFYLSRIRQRGYPVVPGFVVSEEVLRLFLETLNSSESLVANLPNSSLHLDVANWRQLQQ
VAGRLRQEIIHATVPSQWVNTILTAAREWQTNFLIFRPSLTLATKTHRLGNTSGLLDAAFCYCNEDAIAHTLKHTWSQLF
RARSLLYWQRIGVNLQEVNLAILVQPVQNAIASGLLIANSSGCKIQATWGLGLALSQGEIIPDTYQVQQETGIVLERHLG
NKMFAYRLDSSELNDARLSRKEVFTLNHPCIGAYSVQESHQQQYALAEEYLQQVITLGHQLESELGNSFTIEWTIAEENS
TPCLYITQVSTPQSAIPNLRFIKGAGAARGRVTANAYVVNDSQRKPEQLPKGVILVVPAITPDWLSLVEQAVGIVTVQGG
VTSHAAILARELGIPAVVNVADATLLIPNGERLLIDGDRGEVYLLKADEATTVENPHQQPLTPPFHPVVSRQMPMIATQL
LVNLSQPSLIEQVQNLPVDGVGLLRSELMLVPLLKGQHPNFWLLDGRKSELLEQWSEHIIQFARAFAPRPVFYRSLDWRS
HELSANNSQSAPQSILGDRGTFSYVRNSDVFELELTAIANAQKAGYDNIHLLLPFVRSVGEFTFCRQKAEQFGLTQIPQF
QLWMMAEVPSVLFLLPEYVKAGVAGISIGTNDLTQLLLGADREQSDITKVLNERHPAVMAAIAQLIQMSQTAGIPCSICG
QAPAIYPEIIDQLVQWGITSISVEPEAVERTYQAIARAEHRLLLQAARRQVGERG

Sequences:

>Translated_775_residues
MDKLYWLDQIKLQDRAKVGDKAFYLSRIRQRGYPVVPGFVVSEEVLRLFLETLNSSESLVANLPNSSLHLDVANWRQLQQ
VAGRLRQEIIHATVPSQWVNTILTAAREWQTNFLIFRPSLTLATKTHRLGNTSGLLDAAFCYCNEDAIAHTLKHTWSQLF
RARSLLYWQRIGVNLQEVNLAILVQPVQNAIASGLLIANSSGCKIQATWGLGLALSQGEIIPDTYQVQQETGIVLERHLG
NKMFAYRLDSSELNDARLSRKEVFTLNHPCIGAYSVQESHQQQYALAEEYLQQVITLGHQLESELGNSFTIEWTIAEENS
TPCLYITQVSTPQSAIPNLRFIKGAGAARGRVTANAYVVNDSQRKPEQLPKGVILVVPAITPDWLSLVEQAVGIVTVQGG
VTSHAAILARELGIPAVVNVADATLLIPNGERLLIDGDRGEVYLLKADEATTVENPHQQPLTPPFHPVVSRQMPMIATQL
LVNLSQPSLIEQVQNLPVDGVGLLRSELMLVPLLKGQHPNFWLLDGRKSELLEQWSEHIIQFARAFAPRPVFYRSLDWRS
HELSANNSQSAPQSILGDRGTFSYVRNSDVFELELTAIANAQKAGYDNIHLLLPFVRSVGEFTFCRQKAEQFGLTQIPQF
QLWMMAEVPSVLFLLPEYVKAGVAGISIGTNDLTQLLLGADREQSDITKVLNERHPAVMAAIAQLIQMSQTAGIPCSICG
QAPAIYPEIIDQLVQWGITSISVEPEAVERTYQAIARAEHRLLLQAARRQVGERG
>Mature_775_residues
MDKLYWLDQIKLQDRAKVGDKAFYLSRIRQRGYPVVPGFVVSEEVLRLFLETLNSSESLVANLPNSSLHLDVANWRQLQQ
VAGRLRQEIIHATVPSQWVNTILTAAREWQTNFLIFRPSLTLATKTHRLGNTSGLLDAAFCYCNEDAIAHTLKHTWSQLF
RARSLLYWQRIGVNLQEVNLAILVQPVQNAIASGLLIANSSGCKIQATWGLGLALSQGEIIPDTYQVQQETGIVLERHLG
NKMFAYRLDSSELNDARLSRKEVFTLNHPCIGAYSVQESHQQQYALAEEYLQQVITLGHQLESELGNSFTIEWTIAEENS
TPCLYITQVSTPQSAIPNLRFIKGAGAARGRVTANAYVVNDSQRKPEQLPKGVILVVPAITPDWLSLVEQAVGIVTVQGG
VTSHAAILARELGIPAVVNVADATLLIPNGERLLIDGDRGEVYLLKADEATTVENPHQQPLTPPFHPVVSRQMPMIATQL
LVNLSQPSLIEQVQNLPVDGVGLLRSELMLVPLLKGQHPNFWLLDGRKSELLEQWSEHIIQFARAFAPRPVFYRSLDWRS
HELSANNSQSAPQSILGDRGTFSYVRNSDVFELELTAIANAQKAGYDNIHLLLPFVRSVGEFTFCRQKAEQFGLTQIPQF
QLWMMAEVPSVLFLLPEYVKAGVAGISIGTNDLTQLLLGADREQSDITKVLNERHPAVMAAIAQLIQMSQTAGIPCSICG
QAPAIYPEIIDQLVQWGITSISVEPEAVERTYQAIARAEHRLLLQAARRQVGERG

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=825, Percent_Identity=26.0606060606061, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1789193, Length=487, Percent_Identity=26.8993839835729, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI48994992, Length=375, Percent_Identity=25.0666666666667, Blast_Score=93, Evalue=7e-20,
Organism=Escherichia coli, GI1788756, Length=365, Percent_Identity=25.2054794520548, Blast_Score=93, Evalue=8e-20,
Organism=Escherichia coli, GI1788726, Length=373, Percent_Identity=24.6648793565684, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 86168; Mature: 86168

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLYWLDQIKLQDRAKVGDKAFYLSRIRQRGYPVVPGFVVSEEVLRLFLETLNSSESLV
CCCEEEHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
ANLPNSSLHLDVANWRQLQQVAGRLRQEIIHATVPSQWVNTILTAAREWQTNFLIFRPSL
EECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCE
TLATKTHRLGNTSGLLDAAFCYCNEDAIAHTLKHTWSQLFRARSLLYWQRIGVNLQEVNL
EEEEHHHCCCCCCCHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCE
AILVQPVQNAIASGLLIANSSGCKIQATWGLGLALSQGEIIPDTYQVQQETGIVLERHLG
EEEEEHHHHHHHCCEEEECCCCCEEEEECCCEEEECCCCCCCCHHHHHHHCCHHHHHHCC
NKMFAYRLDSSELNDARLSRKEVFTLNHPCIGAYSVQESHQQQYALAEEYLQQVITLGHQ
CCEEEEEECCCCCCHHHCCHHHEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LESELGNSFTIEWTIAEENSTPCLYITQVSTPQSAIPNLRFIKGAGAARGRVTANAYVVN
HHHHCCCEEEEEEEEECCCCCCEEEEEEECCCHHHCCCCEEEECCCCCCCCEEEEEEEEE
DSQRKPEQLPKGVILVVPAITPDWLSLVEQAVGIVTVQGGVTSHAAILARELGIPAVVNV
CCCCCHHHCCCCEEEEEECCCHHHHHHHHHHHCEEEECCCCCHHHHHHHHHCCCCEEEEE
ADATLLIPNGERLLIDGDRGEVYLLKADEATTVENPHQQPLTPPFHPVVSRQMPMIATQL
CCEEEEECCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHH
LVNLSQPSLIEQVQNLPVDGVGLLRSELMLVPLLKGQHPNFWLLDGRKSELLEQWSEHII
HHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHH
QFARAFAPRPVFYRSLDWRSHELSANNSQSAPQSILGDRGTFSYVRNSDVFELELTAIAN
HHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHCCCCEEEEEEHHHHC
AQKAGYDNIHLLLPFVRSVGEFTFCRQKAEQFGLTQIPQFQLWMMAEVPSVLFLLPEYVK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGVAGISIGTNDLTQLLLGADREQSDITKVLNERHPAVMAAIAQLIQMSQTAGIPCSICG
CCCCEEECCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECC
QAPAIYPEIIDQLVQWGITSISVEPEAVERTYQAIARAEHRLLLQAARRQVGERG
CCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDKLYWLDQIKLQDRAKVGDKAFYLSRIRQRGYPVVPGFVVSEEVLRLFLETLNSSESLV
CCCEEEHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
ANLPNSSLHLDVANWRQLQQVAGRLRQEIIHATVPSQWVNTILTAAREWQTNFLIFRPSL
EECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCE
TLATKTHRLGNTSGLLDAAFCYCNEDAIAHTLKHTWSQLFRARSLLYWQRIGVNLQEVNL
EEEEHHHCCCCCCCHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCE
AILVQPVQNAIASGLLIANSSGCKIQATWGLGLALSQGEIIPDTYQVQQETGIVLERHLG
EEEEEHHHHHHHCCEEEECCCCCEEEEECCCEEEECCCCCCCCHHHHHHHCCHHHHHHCC
NKMFAYRLDSSELNDARLSRKEVFTLNHPCIGAYSVQESHQQQYALAEEYLQQVITLGHQ
CCEEEEEECCCCCCHHHCCHHHEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LESELGNSFTIEWTIAEENSTPCLYITQVSTPQSAIPNLRFIKGAGAARGRVTANAYVVN
HHHHCCCEEEEEEEEECCCCCCEEEEEEECCCHHHCCCCEEEECCCCCCCCEEEEEEEEE
DSQRKPEQLPKGVILVVPAITPDWLSLVEQAVGIVTVQGGVTSHAAILARELGIPAVVNV
CCCCCHHHCCCCEEEEEECCCHHHHHHHHHHHCEEEECCCCCHHHHHHHHHCCCCEEEEE
ADATLLIPNGERLLIDGDRGEVYLLKADEATTVENPHQQPLTPPFHPVVSRQMPMIATQL
CCEEEEECCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHH
LVNLSQPSLIEQVQNLPVDGVGLLRSELMLVPLLKGQHPNFWLLDGRKSELLEQWSEHII
HHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHH
QFARAFAPRPVFYRSLDWRSHELSANNSQSAPQSILGDRGTFSYVRNSDVFELELTAIAN
HHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHCCCCEEEEEEHHHHC
AQKAGYDNIHLLLPFVRSVGEFTFCRQKAEQFGLTQIPQFQLWMMAEVPSVLFLLPEYVK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGVAGISIGTNDLTQLLLGADREQSDITKVLNERHPAVMAAIAQLIQMSQTAGIPCSICG
CCCCEEECCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECC
QAPAIYPEIIDQLVQWGITSISVEPEAVERTYQAIARAEHRLLLQAARRQVGERG
CCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7805870 [H]