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The map label for this gene is purB [H]

Identifier: 17230887

GI number: 17230887

Start: 4102742

End: 4104037

Strand: Direct

Name: purB [H]

Synonym: alr3395

Alternate gene names: 17230887

Gene position: 4102742-4104037 (Clockwise)

Preceding gene: 17230886

Following gene: 17230889

Centisome position: 63.97

GC content: 46.99

Gene sequence:

>1296_bases
TTGATAGAGCGTTATACTCTGCCCGAAATGGGCAACCTGTGGACTGACTTTTATAAACTAAAAACCTGGCTTCAGGTGGA
AATTGCAGTCTGTGAAGCCCAAGCGGAATTGGGTTATATTCCATCTGCTGCTGTTGAGGAAATCAAGGCTAAGGCAAATT
TTGATCCGCAACGGGTGTTGGAAATAGAGGCGGAAGTCCGTCACGATGTCATCGCCTTTTTGACTAATGTCAATGAGTAT
GTGGGTGATGCTGGACGTTACATTCACTTGGGCTTAACTAGCTCTGATGTACTAGATACAGCTTTAGCTTTACAACTGGT
TGCTAGTTTGGATGTGTTGGCGCAACGTCTGGAAGATTTGATTCAGGTGATTCGCCAAAAGGCACGGGAACACCGGTATA
CGGTAATGGCTGGCCGATCGCATGGTATTCACGCTGAACCAATCACTTTTGGGTTTAAACTGGCTGGTTGGTTGGCGGAA
GTGTTACGCCACCAACAACGGCTGACAATTTTGCGGGAAACGATCGCCGTGGGTAAGATTTCTGGCGCGGTGGGTACCTA
TGCCAATATTGAACCACGGGTGGAGGCGATCGCTTGTCAAAAACTCGGACTCAAACCCGATACAGCTTCTACACAAGTCA
TTTCCCGCGATATTCATGCTGATTTTGTGCAGCAATTAGCCCTCATCGCTGCTTCTATTGAACGCTTTGCTGTAGAAATT
CGCAATCTACAAAAAACAGACGTTCTGGAAGTAGAGGAATTTTTCTCGAAGGGACAGAAAGGCTCTAGTGCAATGCCACA
CAAACGCAATCCCATCCGTTCGGAACGGCTGACGGGGATGGCGCGGTTAGTGAGGAGTCATGCCGGCGCAGCTTTAGAAG
ATGTCGCTTTGTGGCACGAACGGGACATTTCCCATAGTTCTGTGGAACGAGTGGTTCTGCCAGATGCCTGCATTTTGACC
CATTTCATGCTGATAGAAATCACTAATTTGGTGGAAAATCTGTTAGTTTACCCAGAAAACATGACGCGGAATCTCAACTG
CTATGGTGGCGTTGTATTCAGCCAAAAAGTTTTACTGGCATTGGTAGATAAGGGACTCAGCCGGGAAGAAGCCTATGCGA
TCGTGCAGCAAAATGCTCATACGGCATGGAATAAGCCGGAAGGCAATTTCCACGACTTGATCATTAACGATCCTCGTGTC
ACTCAACATTTGTCGCCCGTAGAAATTGCCGTGTGTTTTGACCCACAGCAACATCTCAAGCATTTGGATGAGGTTTATCA
TAGGTTGGATATTTAG

Upstream 100 bases:

>100_bases
AGTAGTAATTGTTGACTGTTAACAGTTAACGGTCAACTAACAACTAACAACTATCAACCAAAGCGGGGTAAAATATGACA
GTCGTATTCTGCCGCGAACG

Downstream 100 bases:

>100_bases
AATTGGGGATTGGGGACTGGAGGAAAATTTTTCTCTAAGTTCCTCCCCCTGCGACCAACTTCTGAAGATATATTTATCAC
TCAAATCATTAACTATTTTC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTLPEMGNLWTDFYKLKTWLQVEIAVCEAQAELGYIPSAAVEEIKAKANFDPQRVLEIEAEVRHDVIAFLTNVNEY
VGDAGRYIHLGLTSSDVLDTALALQLVASLDVLAQRLEDLIQVIRQKAREHRYTVMAGRSHGIHAEPITFGFKLAGWLAE
VLRHQQRLTILRETIAVGKISGAVGTYANIEPRVEAIACQKLGLKPDTASTQVISRDIHADFVQQLALIAASIERFAVEI
RNLQKTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGMARLVRSHAGAALEDVALWHERDISHSSVERVVLPDACILT
HFMLIEITNLVENLLVYPENMTRNLNCYGGVVFSQKVLLALVDKGLSREEAYAIVQQNAHTAWNKPEGNFHDLIINDPRV
TQHLSPVEIAVCFDPQQHLKHLDEVYHRLDI

Sequences:

>Translated_431_residues
MIERYTLPEMGNLWTDFYKLKTWLQVEIAVCEAQAELGYIPSAAVEEIKAKANFDPQRVLEIEAEVRHDVIAFLTNVNEY
VGDAGRYIHLGLTSSDVLDTALALQLVASLDVLAQRLEDLIQVIRQKAREHRYTVMAGRSHGIHAEPITFGFKLAGWLAE
VLRHQQRLTILRETIAVGKISGAVGTYANIEPRVEAIACQKLGLKPDTASTQVISRDIHADFVQQLALIAASIERFAVEI
RNLQKTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGMARLVRSHAGAALEDVALWHERDISHSSVERVVLPDACILT
HFMLIEITNLVENLLVYPENMTRNLNCYGGVVFSQKVLLALVDKGLSREEAYAIVQQNAHTAWNKPEGNFHDLIINDPRV
TQHLSPVEIAVCFDPQQHLKHLDEVYHRLDI
>Mature_431_residues
MIERYTLPEMGNLWTDFYKLKTWLQVEIAVCEAQAELGYIPSAAVEEIKAKANFDPQRVLEIEAEVRHDVIAFLTNVNEY
VGDAGRYIHLGLTSSDVLDTALALQLVASLDVLAQRLEDLIQVIRQKAREHRYTVMAGRSHGIHAEPITFGFKLAGWLAE
VLRHQQRLTILRETIAVGKISGAVGTYANIEPRVEAIACQKLGLKPDTASTQVISRDIHADFVQQLALIAASIERFAVEI
RNLQKTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGMARLVRSHAGAALEDVALWHERDISHSSVERVVLPDACILT
HFMLIEITNLVENLLVYPENMTRNLNCYGGVVFSQKVLLALVDKGLSREEAYAIVQQNAHTAWNKPEGNFHDLIINDPRV
TQHLSPVEIAVCFDPQQHLKHLDEVYHRLDI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=423, Percent_Identity=29.5508274231678, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI183227688, Length=397, Percent_Identity=29.7229219143577, Blast_Score=158, Evalue=9e-39,
Organism=Escherichia coli, GI1787376, Length=330, Percent_Identity=28.1818181818182, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17508577, Length=380, Percent_Identity=29.4736842105263, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564234, Length=347, Percent_Identity=30.2593659942363, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6323391, Length=410, Percent_Identity=29.0243902439024, Blast_Score=157, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24647570, Length=424, Percent_Identity=27.8301886792453, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48493; Mature: 48493

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTLPEMGNLWTDFYKLKTWLQVEIAVCEAQAELGYIPSAAVEEIKAKANFDPQRVL
CCCCCCCCCHHHHHHHHHHHHHHHHEEEHEEHHHHHCCCCCHHHHHHHHHHCCCCHHHHH
EIEAEVRHDVIAFLTNVNEYVGDAGRYIHLGLTSSDVLDTALALQLVASLDVLAQRLEDL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IQVIRQKAREHRYTVMAGRSHGIHAEPITFGFKLAGWLAEVLRHQQRLTILRETIAVGKI
HHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANIEPRVEAIACQKLGLKPDTASTQVISRDIHADFVQQLALIAASIERFAVEI
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQKTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGMARLVRSHAGAALEDVALWHE
HCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RDISHSSVERVVLPDACILTHFMLIEITNLVENLLVYPENMTRNLNCYGGVVFSQKVLLA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHH
LVDKGLSREEAYAIVQQNAHTAWNKPEGNFHDLIINDPRVTQHLSPVEIAVCFDPQQHLK
HHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHCCCCEEEEEEECHHHHHH
HLDEVYHRLDI
HHHHHHHHHCC
>Mature Secondary Structure
MIERYTLPEMGNLWTDFYKLKTWLQVEIAVCEAQAELGYIPSAAVEEIKAKANFDPQRVL
CCCCCCCCCHHHHHHHHHHHHHHHHEEEHEEHHHHHCCCCCHHHHHHHHHHCCCCHHHHH
EIEAEVRHDVIAFLTNVNEYVGDAGRYIHLGLTSSDVLDTALALQLVASLDVLAQRLEDL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IQVIRQKAREHRYTVMAGRSHGIHAEPITFGFKLAGWLAEVLRHQQRLTILRETIAVGKI
HHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANIEPRVEAIACQKLGLKPDTASTQVISRDIHADFVQQLALIAASIERFAVEI
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQKTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGMARLVRSHAGAALEDVALWHE
HCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RDISHSSVERVVLPDACILTHFMLIEITNLVENLLVYPENMTRNLNCYGGVVFSQKVLLA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHH
LVDKGLSREEAYAIVQQNAHTAWNKPEGNFHDLIINDPRVTQHLSPVEIAVCFDPQQHLK
HHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHCCCCEEEEEEECHHHHHH
HLDEVYHRLDI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]