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The map label for this gene is recF [H]

Identifier: 17230866

GI number: 17230866

Start: 4082801

End: 4083931

Strand: Reverse

Name: recF [H]

Synonym: all3374

Alternate gene names: 17230866

Gene position: 4083931-4082801 (Counterclockwise)

Preceding gene: 17230867

Following gene: 17230865

Centisome position: 63.67

GC content: 46.07

Gene sequence:

>1131_bases
ATGTACCTCAAGACTCTACACCTCCGACATTTTCGCAACTACTACGACCAAAAGGTTGAGTTTACGGCTGCCAAAACTAT
TTTGGTGGGTAATAACGCTCAGGGAAAGTCGAATTTGTTGGAGGCTGTGGAGTTATTGGCGACATTGCGATCGCACCGGA
TGGCACGCGATCGCGATTTCGTCCAAGAAGAGGAACCAGTAGCCCAAATAAACGCCACTTTGGAGCGAGACACAGGGGTT
AGTGACCTCAGTCTGATTCTGCGGCGTAATGGTCGCCGCACGGTCGCCCTGAATGGGGAACCTCTGCGGCGACAAATGGA
TTTTTTAGGTGTGTTAAATGCGGTGCAGTTCTCCAGTTTGGATTTAGAACTGGTGCGTGGTAGTCCTGAAGTCCGGCGCA
ACTGGCTGGATACTCTCCTGATTCAGCTTGAGCCAGTTTATGCCCATATTTTGCAGCAATATAATCAGGTGTTACGTCAA
CGCAATGCCTATTTAAAGAAGTTGCAAGACTCAGCACTCACTACTCAAGACTCAGCCCTGGCTATTTGGGACGCGCAATT
GGTTACTACAGGAACAAAGGTAATTAGGAGACGCGATCGCGCTTTAGCTCGACTTGCCCCCCTAGCTACTGCTTGGCACA
CCAGTATTAGTGGTAGTACAGAAGTTCTGCAAATCAACTATACGCCTAATGTGCAATTGGTGAAAAATCAACCCGAAGAG
GTGCAGCAAGCTTTTTTAAGCCAGTTACAGCAACGAGCCGTTCCCGAAATTTACCGAGGTACTACCCTTGTCGGCCCCCA
TCGGGACGAAGTAGAATTAACTATCAATCAAACTCCAGCCCGTCAATACGGTTCTCAGGGTCAGCAACGCACGCTGGTAT
TAGCTCTCAAACTCGCAGAACTGCAATTAATTGAGGAAGTTGTTAAAGAACCTCCTTTACTCTTACTTGATGATGTTTTG
GCAGAATTAGATCCATCTCGCCAGAATCAATTACTGGATACAATTCAAGACAGATTTCAAACCTTAATTACTACAACTCA
CCTCAGTTCTTTTGATGCCCAGTGGTTAAACTCATCGCAAATCCTTTTTGTTGAGCAAGGTAAAATATCTACATCTAATG
CTGTTAGATAA

Upstream 100 bases:

>100_bases
AGAGGACTGTTTAGCAATTCAAAATTCAAAATTCAAAATCTCCCGTTTTGAGTTTTAAATTTTGCGTTTTTAATTCTGTC
TGATATCCCTATTGTTTTTT

Downstream 100 bases:

>100_bases
TATTAAATAATCAGCTTATTTAACCACCATTAATTTTTCCATAAATATAGCATCTACTATCTTGAATGATTAAAATACTC
CCAACTTTTAAGTATGAAAA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MYLKTLHLRHFRNYYDQKVEFTAAKTILVGNNAQGKSNLLEAVELLATLRSHRMARDRDFVQEEEPVAQINATLERDTGV
SDLSLILRRNGRRTVALNGEPLRRQMDFLGVLNAVQFSSLDLELVRGSPEVRRNWLDTLLIQLEPVYAHILQQYNQVLRQ
RNAYLKKLQDSALTTQDSALAIWDAQLVTTGTKVIRRRDRALARLAPLATAWHTSISGSTEVLQINYTPNVQLVKNQPEE
VQQAFLSQLQQRAVPEIYRGTTLVGPHRDEVELTINQTPARQYGSQGQQRTLVLALKLAELQLIEEVVKEPPLLLLDDVL
AELDPSRQNQLLDTIQDRFQTLITTTHLSSFDAQWLNSSQILFVEQGKISTSNAVR

Sequences:

>Translated_376_residues
MYLKTLHLRHFRNYYDQKVEFTAAKTILVGNNAQGKSNLLEAVELLATLRSHRMARDRDFVQEEEPVAQINATLERDTGV
SDLSLILRRNGRRTVALNGEPLRRQMDFLGVLNAVQFSSLDLELVRGSPEVRRNWLDTLLIQLEPVYAHILQQYNQVLRQ
RNAYLKKLQDSALTTQDSALAIWDAQLVTTGTKVIRRRDRALARLAPLATAWHTSISGSTEVLQINYTPNVQLVKNQPEE
VQQAFLSQLQQRAVPEIYRGTTLVGPHRDEVELTINQTPARQYGSQGQQRTLVLALKLAELQLIEEVVKEPPLLLLDDVL
AELDPSRQNQLLDTIQDRFQTLITTTHLSSFDAQWLNSSQILFVEQGKISTSNAVR
>Mature_376_residues
MYLKTLHLRHFRNYYDQKVEFTAAKTILVGNNAQGKSNLLEAVELLATLRSHRMARDRDFVQEEEPVAQINATLERDTGV
SDLSLILRRNGRRTVALNGEPLRRQMDFLGVLNAVQFSSLDLELVRGSPEVRRNWLDTLLIQLEPVYAHILQQYNQVLRQ
RNAYLKKLQDSALTTQDSALAIWDAQLVTTGTKVIRRRDRALARLAPLATAWHTSISGSTEVLQINYTPNVQLVKNQPEE
VQQAFLSQLQQRAVPEIYRGTTLVGPHRDEVELTINQTPARQYGSQGQQRTLVLALKLAELQLIEEVVKEPPLLLLDDVL
AELDPSRQNQLLDTIQDRFQTLITTTHLSSFDAQWLNSSQILFVEQGKISTSNAVR

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=375, Percent_Identity=22.9333333333333, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 42788; Mature: 42788

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2 ; PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLKTLHLRHFRNYYDQKVEFTAAKTILVGNNAQGKSNLLEAVELLATLRSHRMARDRDF
CCEEHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VQEEEPVAQINATLERDTGVSDLSLILRRNGRRTVALNGEPLRRQMDFLGVLNAVQFSSL
HHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCC
DLELVRGSPEVRRNWLDTLLIQLEPVYAHILQQYNQVLRQRNAYLKKLQDSALTTQDSAL
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
AIWDAQLVTTGTKVIRRRDRALARLAPLATAWHTSISGSTEVLQINYTPNVQLVKNQPEE
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCHH
VQQAFLSQLQQRAVPEIYRGTTLVGPHRDEVELTINQTPARQYGSQGQQRTLVLALKLAE
HHHHHHHHHHHHCCHHHHCCCEEECCCCCCEEEEEECCCHHHHCCCCCCEEEHHHHHHHH
LQLIEEVVKEPPLLLLDDVLAELDPSRQNQLLDTIQDRFQTLITTTHLSSFDAQWLNSSQ
HHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCC
ILFVEQGKISTSNAVR
EEEEECCCCCCCCCCC
>Mature Secondary Structure
MYLKTLHLRHFRNYYDQKVEFTAAKTILVGNNAQGKSNLLEAVELLATLRSHRMARDRDF
CCEEHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VQEEEPVAQINATLERDTGVSDLSLILRRNGRRTVALNGEPLRRQMDFLGVLNAVQFSSL
HHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCC
DLELVRGSPEVRRNWLDTLLIQLEPVYAHILQQYNQVLRQRNAYLKKLQDSALTTQDSAL
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
AIWDAQLVTTGTKVIRRRDRALARLAPLATAWHTSISGSTEVLQINYTPNVQLVKNQPEE
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCHH
VQQAFLSQLQQRAVPEIYRGTTLVGPHRDEVELTINQTPARQYGSQGQQRTLVLALKLAE
HHHHHHHHHHHHCCHHHHCCCEEECCCCCCEEEEEECCCHHHHCCCCCCEEEHHHHHHHH
LQLIEEVVKEPPLLLLDDVLAELDPSRQNQLLDTIQDRFQTLITTTHLSSFDAQWLNSSQ
HHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCC
ILFVEQGKISTSNAVR
EEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA