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The map label for this gene is mraW [H]

Identifier: 17230846

GI number: 17230846

Start: 4059043

End: 4059951

Strand: Reverse

Name: mraW [H]

Synonym: all3354

Alternate gene names: 17230846

Gene position: 4059951-4059043 (Counterclockwise)

Preceding gene: 17230851

Following gene: 17230841

Centisome position: 63.3

GC content: 44.77

Gene sequence:

>909_bases
ATGACTAAAACACCGCTCACTATAGAAGAACCGATATTTTCTCATCTACCTGTTTTACCGCAAGAAGTGATTACAGGTCT
GGTGGTGCGTCCTGGTGGGCGTTATTTGGATGTAACGGTAGGGGGTGGTGGTCACAGCCGGTTGATTTTAGAGGCTGCGC
CAGATGTCAAGCTAACAGCAGTTGACCAAGATGGGGATGCTTTAACAGCCGCGAAGCAAGAGCTGGCAGAATTTGGGGAA
CAAGTAAAGTTTGTGCGGAGTAATTTTGCTGCTTATGATTTCCCGTCCACTAGTTTCGATGGTGTACTGGCAGATTTAGG
CGTGAGTTCTTATCACCTAGATACCCCAGAGCGTGGTTTTAGCTTCCGTCATCAAGCGAGTCTCGATATGCGGATGGATC
AAAGACAATCTCTTAGCGCCGCCGATGTCATTAATGATTGGGACGAAGTTGAGTTGGCAAATATTTTCTTCAAATATGGT
GAAGAAAGGCTCTCACGACGTATTGCTAGACGGATTGTGGAAAAACGTCCTTTTCATACAACTACAGAATTAGCTGAGGC
GATCGCCTCTTCTGTTCCCCCCAAATACCGCTATGGTAGGATTCATCCTGCTACCCGTGTATTTCAAGCTTTGCGAATTG
TCGTCAATGATGAGTTGAAGTCCTTAGAAACTTTTATAGAAAAGGCTCCCAAAGCTCTTGTCCCTGGTGGCAGAATTGCC
ATTATCAGTTTTCACAGTTTGGAAGACCGTTTAGTAAAACACGGTTTAAGAAATTCACCTTTATTAAAAGTTTTAACGAA
AAAACCCATTATTGCTACCGACGACGAGATTGCAAATAATCCGCGATCGCGTTCCGCCAAACTCAGGATAGCAGAGAAGC
AGGCTGAGACGGGAGACGAAGATAATTAA

Upstream 100 bases:

>100_bases
TTGTTTCACTTCTTCAATTATTATAGGAAAGCTCGATATTTAAGAAATTGAGGCGATGGAATTTTTTAACTGTTGACCAT
TGACTATTGACTAACGACTA

Downstream 100 bases:

>100_bases
AAAATAAAAGAGGGGAGTTACGTTAACCCCCCTCTACAAACAAAGAAATTATTCAGTCGAAGATAGCCAGGAACTATATG
CGGTTGCTGGTGGTGGCTGG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTKTPLTIEEPIFSHLPVLPQEVITGLVVRPGGRYLDVTVGGGGHSRLILEAAPDVKLTAVDQDGDALTAAKQELAEFGE
QVKFVRSNFAAYDFPSTSFDGVLADLGVSSYHLDTPERGFSFRHQASLDMRMDQRQSLSAADVINDWDEVELANIFFKYG
EERLSRRIARRIVEKRPFHTTTELAEAIASSVPPKYRYGRIHPATRVFQALRIVVNDELKSLETFIEKAPKALVPGGRIA
IISFHSLEDRLVKHGLRNSPLLKVLTKKPIIATDDEIANNPRSRSAKLRIAEKQAETGDEDN

Sequences:

>Translated_302_residues
MTKTPLTIEEPIFSHLPVLPQEVITGLVVRPGGRYLDVTVGGGGHSRLILEAAPDVKLTAVDQDGDALTAAKQELAEFGE
QVKFVRSNFAAYDFPSTSFDGVLADLGVSSYHLDTPERGFSFRHQASLDMRMDQRQSLSAADVINDWDEVELANIFFKYG
EERLSRRIARRIVEKRPFHTTTELAEAIASSVPPKYRYGRIHPATRVFQALRIVVNDELKSLETFIEKAPKALVPGGRIA
IISFHSLEDRLVKHGLRNSPLLKVLTKKPIIATDDEIANNPRSRSAKLRIAEKQAETGDEDN
>Mature_301_residues
TKTPLTIEEPIFSHLPVLPQEVITGLVVRPGGRYLDVTVGGGGHSRLILEAAPDVKLTAVDQDGDALTAAKQELAEFGEQ
VKFVRSNFAAYDFPSTSFDGVLADLGVSSYHLDTPERGFSFRHQASLDMRMDQRQSLSAADVINDWDEVELANIFFKYGE
ERLSRRIARRIVEKRPFHTTTELAEAIASSVPPKYRYGRIHPATRVFQALRIVVNDELKSLETFIEKAPKALVPGGRIAI
ISFHSLEDRLVKHGLRNSPLLKVLTKKPIIATDDEIANNPRSRSAKLRIAEKQAETGDEDN

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=38.3928571428571, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=40.2116402116402, Blast_Score=124, Evalue=9e-29,
Organism=Escherichia coli, GI1786270, Length=316, Percent_Identity=41.1392405063291, Blast_Score=206, Evalue=1e-54,
Organism=Drosophila melanogaster, GI62472493, Length=328, Percent_Identity=36.5853658536585, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI28571637, Length=328, Percent_Identity=36.5853658536585, Blast_Score=164, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 33613; Mature: 33481

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00862 OX2_COVAL_FAD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTPLTIEEPIFSHLPVLPQEVITGLVVRPGGRYLDVTVGGGGHSRLILEAAPDVKLTA
CCCCCCCCCCCHHHHCCCCHHHHHCCEEECCCCCEEEEEECCCCCCEEEEEECCCEEEEE
VDQDGDALTAAKQELAEFGEQVKFVRSNFAAYDFPSTSFDGVLADLGVSSYHLDTPERGF
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHCCCCCEECCCCCCCC
SFRHQASLDMRMDQRQSLSAADVINDWDEVELANIFFKYGEERLSRRIARRIVEKRPFHT
CCCCCCCCCHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TTELAEAIASSVPPKYRYGRIHPATRVFQALRIVVNDELKSLETFIEKAPKALVPGGRIA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEE
IISFHSLEDRLVKHGLRNSPLLKVLTKKPIIATDDEIANNPRSRSAKLRIAEKQAETGDE
EEEEHHHHHHHHHCCCCCCCHHHHHHCCCCEECCHHHHCCCCCCCCEEEEEHHHHCCCCC
DN
CC
>Mature Secondary Structure 
TKTPLTIEEPIFSHLPVLPQEVITGLVVRPGGRYLDVTVGGGGHSRLILEAAPDVKLTA
CCCCCCCCCCHHHHCCCCHHHHHCCEEECCCCCEEEEEECCCCCCEEEEEECCCEEEEE
VDQDGDALTAAKQELAEFGEQVKFVRSNFAAYDFPSTSFDGVLADLGVSSYHLDTPERGF
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHCCCCCEECCCCCCCC
SFRHQASLDMRMDQRQSLSAADVINDWDEVELANIFFKYGEERLSRRIARRIVEKRPFHT
CCCCCCCCCHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TTELAEAIASSVPPKYRYGRIHPATRVFQALRIVVNDELKSLETFIEKAPKALVPGGRIA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEE
IISFHSLEDRLVKHGLRNSPLLKVLTKKPIIATDDEIANNPRSRSAKLRIAEKQAETGDE
EEEEHHHHHHHHHCCCCCCCHHHHHHCCCCEECCHHHHCCCCCCCCEEEEEHHHHCCCCC
DN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA