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The map label for this gene is tyrS [H]

Identifier: 17230474

GI number: 17230474

Start: 3623785

End: 3624981

Strand: Direct

Name: tyrS [H]

Synonym: alr2982

Alternate gene names: 17230474

Gene position: 3623785-3624981 (Clockwise)

Preceding gene: 17230472

Following gene: 17230475

Centisome position: 56.5

GC content: 44.78

Gene sequence:

>1197_bases
ATGGCGGAAAATTTTTCTTGGCTACATCGTGGGATAGCGGAAGTTTTCCCACAACCTACTGATGCTGAAAGTGACATCGA
GAGTCTAGAGAAACGCCTAGCAACTACGGATCGACCTCTACGGGTTAAGTATGGTATTGACCCGACAGGAGCCGATATTC
ATCTTGGTCATAGCATACCAATGCGTAAACTGCGAGGGTTTCAAGATGCTGGTCATACGGCAGTGTTAATTATTGGTGAT
TTTACGGCACGTATTGGCGACCCGACAGGTAAGTCTGAGATGCGTCGTCAACTGACAGAGGAAGATGTGAAGCAAAATGC
TCAAACCTACCTCGACCAAGTGCGTCCTATTTTGGATTTTGACACACCCGGAAGATTAGAGGTACGCTATAACTCAGAAT
GGCTTTCCCGGCTTGATTTAGGGAAAATTACTGAGTTACTTGCCACCATGACCGTGGGGCAAATGTTAGCCAAGGAAGGC
TTTGCCGATCGCTATAAAAAAGAGAATCCAATTTTCCTGCATGAGTTCCTTTATCCTTTGATGCAGGGGTATGATTCGGT
GGCTATTGAGGCAGATGTGGAATTAGGCGGCACAGACCAGAAGTTTAACATTGCTGTTGGCCGTGATTTACAGCGACACT
TTGGTCAAAAGCCCCAGTTTGGTGTACTTCTACCAATTTTAATTGGTACTGATGGCGTGCAGAAAATGTCCAAGTCTTTA
GGTAATTACGTGAGTTTGTCTGAACACCCAGGACAAAAATACCAAAAGTTACAAGGCGTTCCCGATGTTCTGCTAAAAGA
CTATTTTGAATTATTGACTAATTTACCCATTGATGCACTGCCAGAAAATCCGCGCGATCGCCAAATGCTCTTAGCTTGGG
AGATTGTCAAACAATACCACGGTGAACAAGCAGCCAATGAAGCTAAAGAAGCGGCTCAAAGTGGCGGTAAAGAAGGTGCA
GTCCCAGAATTTTCTCTATCTAATGTCTCTCAATTCCCCGTCAAGTTAGCTTATTTACTTGGTGCGACTGGCTTGTGTAA
AAGTACAGGGGAAGGTAAACGGAAAATTCAAGAAGGCGGAGTACGCTTAGATGGCGATCGCATTTCTGATGCCGATACCA
CCTTTCAACAGCCAGATGAACTCCAAGGGCGAGTGTTACAAGTAGGGAAAAATAAGTTTATGCGCTTAGTACCTTAG

Upstream 100 bases:

>100_bases
TATTAGTTTGGCAGATGAAATAACGGACAATTTGTCTCGTGAACATAACCAACCCAATTTCTCTAGTAAAAAACGTAGTT
AATAGCGAATCTCTTAAAGT

Downstream 100 bases:

>100_bases
AGGAGATAGGGGTATAAGGGTGTAAGGGTGTAGGGGGAAATTAATGAGTAATGACAAAATAATTGTGGCTTTGGATGTGC
CAGATACGGAGAGTGCGATC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAENFSWLHRGIAEVFPQPTDAESDIESLEKRLATTDRPLRVKYGIDPTGADIHLGHSIPMRKLRGFQDAGHTAVLIIGD
FTARIGDPTGKSEMRRQLTEEDVKQNAQTYLDQVRPILDFDTPGRLEVRYNSEWLSRLDLGKITELLATMTVGQMLAKEG
FADRYKKENPIFLHEFLYPLMQGYDSVAIEADVELGGTDQKFNIAVGRDLQRHFGQKPQFGVLLPILIGTDGVQKMSKSL
GNYVSLSEHPGQKYQKLQGVPDVLLKDYFELLTNLPIDALPENPRDRQMLLAWEIVKQYHGEQAANEAKEAAQSGGKEGA
VPEFSLSNVSQFPVKLAYLLGATGLCKSTGEGKRKIQEGGVRLDGDRISDADTTFQQPDELQGRVLQVGKNKFMRLVP

Sequences:

>Translated_398_residues
MAENFSWLHRGIAEVFPQPTDAESDIESLEKRLATTDRPLRVKYGIDPTGADIHLGHSIPMRKLRGFQDAGHTAVLIIGD
FTARIGDPTGKSEMRRQLTEEDVKQNAQTYLDQVRPILDFDTPGRLEVRYNSEWLSRLDLGKITELLATMTVGQMLAKEG
FADRYKKENPIFLHEFLYPLMQGYDSVAIEADVELGGTDQKFNIAVGRDLQRHFGQKPQFGVLLPILIGTDGVQKMSKSL
GNYVSLSEHPGQKYQKLQGVPDVLLKDYFELLTNLPIDALPENPRDRQMLLAWEIVKQYHGEQAANEAKEAAQSGGKEGA
VPEFSLSNVSQFPVKLAYLLGATGLCKSTGEGKRKIQEGGVRLDGDRISDADTTFQQPDELQGRVLQVGKNKFMRLVP
>Mature_397_residues
AENFSWLHRGIAEVFPQPTDAESDIESLEKRLATTDRPLRVKYGIDPTGADIHLGHSIPMRKLRGFQDAGHTAVLIIGDF
TARIGDPTGKSEMRRQLTEEDVKQNAQTYLDQVRPILDFDTPGRLEVRYNSEWLSRLDLGKITELLATMTVGQMLAKEGF
ADRYKKENPIFLHEFLYPLMQGYDSVAIEADVELGGTDQKFNIAVGRDLQRHFGQKPQFGVLLPILIGTDGVQKMSKSLG
NYVSLSEHPGQKYQKLQGVPDVLLKDYFELLTNLPIDALPENPRDRQMLLAWEIVKQYHGEQAANEAKEAAQSGGKEGAV
PEFSLSNVSQFPVKLAYLLGATGLCKSTGEGKRKIQEGGVRLDGDRISDADTTFQQPDELQGRVLQVGKNKFMRLVP

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=334, Percent_Identity=29.3413173652695, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1787925, Length=406, Percent_Identity=29.3103448275862, Blast_Score=157, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI25145802, Length=383, Percent_Identity=26.1096605744125, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6325160, Length=436, Percent_Identity=25.6880733944954, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20130383, Length=324, Percent_Identity=27.7777777777778, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44439; Mature: 44308

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENFSWLHRGIAEVFPQPTDAESDIESLEKRLATTDRPLRVKYGIDPTGADIHLGHSIP
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCC
MRKLRGFQDAGHTAVLIIGDFTARIGDPTGKSEMRRQLTEEDVKQNAQTYLDQVRPILDF
HHHHCCCCCCCCEEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DTPGRLEVRYNSEWLSRLDLGKITELLATMTVGQMLAKEGFADRYKKENPIFLHEFLYPL
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCEEHHHHHHHH
MQGYDSVAIEADVELGGTDQKFNIAVGRDLQRHFGQKPQFGVLLPILIGTDGVQKMSKSL
HCCCCCEEEEEEEEECCCCCEEEEECCCHHHHHCCCCCCCCEEEHHHHCCHHHHHHHHHH
GNYVSLSEHPGQKYQKLQGVPDVLLKDYFELLTNLPIDALPENPRDRQMLLAWEIVKQYH
CCEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
GEQAANEAKEAAQSGGKEGAVPEFSLSNVSQFPVKLAYLLGATGLCKSTGEGKRKIQEGG
CHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHCC
VRLDGDRISDADTTFQQPDELQGRVLQVGKNKFMRLVP
CEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEECCC
>Mature Secondary Structure 
AENFSWLHRGIAEVFPQPTDAESDIESLEKRLATTDRPLRVKYGIDPTGADIHLGHSIP
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCC
MRKLRGFQDAGHTAVLIIGDFTARIGDPTGKSEMRRQLTEEDVKQNAQTYLDQVRPILDF
HHHHCCCCCCCCEEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DTPGRLEVRYNSEWLSRLDLGKITELLATMTVGQMLAKEGFADRYKKENPIFLHEFLYPL
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCEEHHHHHHHH
MQGYDSVAIEADVELGGTDQKFNIAVGRDLQRHFGQKPQFGVLLPILIGTDGVQKMSKSL
HCCCCCEEEEEEEEECCCCCEEEEECCCHHHHHCCCCCCCCEEEHHHHCCHHHHHHHHHH
GNYVSLSEHPGQKYQKLQGVPDVLLKDYFELLTNLPIDALPENPRDRQMLLAWEIVKQYH
CCEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
GEQAANEAKEAAQSGGKEGAVPEFSLSNVSQFPVKLAYLLGATGLCKSTGEGKRKIQEGG
CHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHCC
VRLDGDRISDADTTFQQPDELQGRVLQVGKNKFMRLVP
CEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA