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The map label for this gene is lhgO [H]

Identifier: 17230318

GI number: 17230318

Start: 3442031

End: 3443245

Strand: Direct

Name: lhgO [H]

Synonym: alr2826

Alternate gene names: 17230318

Gene position: 3442031-3443245 (Clockwise)

Preceding gene: 17230317

Following gene: 17230319

Centisome position: 53.67

GC content: 44.28

Gene sequence:

>1215_bases
ATGTACGATTTTACCATTGTTGGCGGGGGCATAGTTGGGCTATCTACAGGGATGGCTTTAGGTAAGCGTTATCCTCAAGC
AAGAATTTTAGTATTAGAAAAAGAAAGCCAGTGGGCATTTCACCAAACTGGTAATAATAGTGGTGTAATTCACTCTGGAA
TTTATTACAAGCCAGGTAGTTTTAAAGCTAAATTTTGCCGTGATGGTAGAGACTCAATGGTAAAATTTTGCCAGGATTAT
GGCATCGACCATGAGGTTTGTGGCAAGGTAATTGTAGCGACAAATGAGCAGGAGTTACCACGCCTAGAAAACCTTTACCA
ACGTGGCTTGGAAAATGGTACACAAGTTCAGAAAATTAGCGCCGAAGAAGTCAAGGAAATTGAACCTTACGTTAAATGTG
TAGCGGGGATTCGGGTATTTTCTACTGGTATTGTTAATTATAAGCAAGTTTGCTTAAAATACGTCGAATTAATTCAACAA
CAAGGCGGAGATTTAAGGCTTAACACCAAAGTTTTAAAAATTTCCCCCAGTGGTAACAATCACGTACTGGAAACTAATAA
CGGTAACTTTGAAACCAGATTTGTGATCAATTGTGCCGGATTGCACAGCGATCGCATCGCTAAGTTAGGTGGAGTCAAAC
CAAGCGCCAAAATCGTACCCTTCCGGGGAGAATATTACGAACTAACCCCCGAAAAACGGTATTTGGTCAAAACACTCATT
TACCCGGTTCCCAACCCTGAATTTCCCTTCCTGGGTGTCCATTTCACCCGCATGATTGACGGAAGTGTCCACGCGGGGCC
GAATGCAGTTTTGAGTCTGAAGCGCGAAGGTTACAAAAAAACCGACTTTGACCTGAGAGACTTTGCCGAAGTGATGACCT
ACCCTGGCTTTTGGAAACTTGCAGGCAAACACGCCGACGAAGGTATCCAAGAAATCATTCGTTCCTTCAGCAAAGCCGCC
TTCACCAGAAGTTTGCAAAACCTCATTCCCGAAGTCCAAGCCGAGGATTTAGTTCCCACCCATGCAGGGGTACGCGCCCA
AGCCTTAATGGATGATGGCAAACTTGTAGACGACTTCTACATTGTTCCTGGTGAAAACTCCATTCATGTTTGCAATGCAC
CTTCACCAGCCGCGACTTCTTCCCTAGAAATTGGCAAAGCGATCGCTGCACAAATCCCCCAGCAATCACACCTTGAGAAT
GTAGTGATTGCATAG

Upstream 100 bases:

>100_bases
ACGGAATTCAAAAAACATTTTGGCATTTTGTCCTATCTTATTTTGACTTCCGTTGTAAGGTATTCTTTACTGATTTAGTG
TAATTTTTGCAGTAATAACA

Downstream 100 bases:

>100_bases
ATAGTTAAGCAAGGGGCAGGGTTTATTACTCAGCCCTCCTCACTCCCCATTAACAAATATAGAGAATTTGACACAAATGA
AAATTTTAGTAACCGGAACC

Product: hydroxyglutarate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MYDFTIVGGGIVGLSTGMALGKRYPQARILVLEKESQWAFHQTGNNSGVIHSGIYYKPGSFKAKFCRDGRDSMVKFCQDY
GIDHEVCGKVIVATNEQELPRLENLYQRGLENGTQVQKISAEEVKEIEPYVKCVAGIRVFSTGIVNYKQVCLKYVELIQQ
QGGDLRLNTKVLKISPSGNNHVLETNNGNFETRFVINCAGLHSDRIAKLGGVKPSAKIVPFRGEYYELTPEKRYLVKTLI
YPVPNPEFPFLGVHFTRMIDGSVHAGPNAVLSLKREGYKKTDFDLRDFAEVMTYPGFWKLAGKHADEGIQEIIRSFSKAA
FTRSLQNLIPEVQAEDLVPTHAGVRAQALMDDGKLVDDFYIVPGENSIHVCNAPSPAATSSLEIGKAIAAQIPQQSHLEN
VVIA

Sequences:

>Translated_404_residues
MYDFTIVGGGIVGLSTGMALGKRYPQARILVLEKESQWAFHQTGNNSGVIHSGIYYKPGSFKAKFCRDGRDSMVKFCQDY
GIDHEVCGKVIVATNEQELPRLENLYQRGLENGTQVQKISAEEVKEIEPYVKCVAGIRVFSTGIVNYKQVCLKYVELIQQ
QGGDLRLNTKVLKISPSGNNHVLETNNGNFETRFVINCAGLHSDRIAKLGGVKPSAKIVPFRGEYYELTPEKRYLVKTLI
YPVPNPEFPFLGVHFTRMIDGSVHAGPNAVLSLKREGYKKTDFDLRDFAEVMTYPGFWKLAGKHADEGIQEIIRSFSKAA
FTRSLQNLIPEVQAEDLVPTHAGVRAQALMDDGKLVDDFYIVPGENSIHVCNAPSPAATSSLEIGKAIAAQIPQQSHLEN
VVIA
>Mature_404_residues
MYDFTIVGGGIVGLSTGMALGKRYPQARILVLEKESQWAFHQTGNNSGVIHSGIYYKPGSFKAKFCRDGRDSMVKFCQDY
GIDHEVCGKVIVATNEQELPRLENLYQRGLENGTQVQKISAEEVKEIEPYVKCVAGIRVFSTGIVNYKQVCLKYVELIQQ
QGGDLRLNTKVLKISPSGNNHVLETNNGNFETRFVINCAGLHSDRIAKLGGVKPSAKIVPFRGEYYELTPEKRYLVKTLI
YPVPNPEFPFLGVHFTRMIDGSVHAGPNAVLSLKREGYKKTDFDLRDFAEVMTYPGFWKLAGKHADEGIQEIIRSFSKAA
FTRSLQNLIPEVQAEDLVPTHAGVRAQALMDDGKLVDDFYIVPGENSIHVCNAPSPAATSSLEIGKAIAAQIPQQSHLEN
VVIA

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=403, Percent_Identity=45.6575682382134, Blast_Score=338, Evalue=4e-93,
Organism=Escherichia coli, GI87082148, Length=393, Percent_Identity=46.8193384223919, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17565764, Length=401, Percent_Identity=44.6384039900249, Blast_Score=331, Evalue=5e-91,
Organism=Drosophila melanogaster, GI24585081, Length=387, Percent_Identity=42.8940568475452, Blast_Score=302, Evalue=3e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 44753; Mature: 44753

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDFTIVGGGIVGLSTGMALGKRYPQARILVLEKESQWAFHQTGNNSGVIHSGIYYKPGS
CCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCEEECCEEECCCC
FKAKFCRDGRDSMVKFCQDYGIDHEVCGKVIVATNEQELPRLENLYQRGLENGTQVQKIS
CCHHHHCCCHHHHHHHHHHHCCCHHHCCEEEEECCHHCCHHHHHHHHHHCCCCCCEECCC
AEEVKEIEPYVKCVAGIRVFSTGIVNYKQVCLKYVELIQQQGGDLRLNTKVLKISPSGNN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCC
HVLETNNGNFETRFVINCAGLHSDRIAKLGGVKPSAKIVPFRGEYYELTPEKRYLVKTLI
EEEECCCCCEEEEEEEEEECCCCHHHHHHCCCCCCCEEEECCCCEEEECCCHHHHHHHHC
YPVPNPEFPFLGVHFTRMIDGSVHAGPNAVLSLKREGYKKTDFDLRDFAEVMTYPGFWKL
CCCCCCCCCEEEEEEEEEECCCCCCCCHHEEEEHHCCCCCCCCCHHHHHHHHCCCCCEEC
AGKHADEGIQEIIRSFSKAAFTRSLQNLIPEVQAEDLVPTHAGVRAQALMDDGKLVDDFY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCEEEEECCCCCEEEEEE
IVPGENSIHVCNAPSPAATSSLEIGKAIAAQIPQQSHLENVVIA
EECCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCHHCCCCEECC
>Mature Secondary Structure
MYDFTIVGGGIVGLSTGMALGKRYPQARILVLEKESQWAFHQTGNNSGVIHSGIYYKPGS
CCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCEEECCEEECCCC
FKAKFCRDGRDSMVKFCQDYGIDHEVCGKVIVATNEQELPRLENLYQRGLENGTQVQKIS
CCHHHHCCCHHHHHHHHHHHCCCHHHCCEEEEECCHHCCHHHHHHHHHHCCCCCCEECCC
AEEVKEIEPYVKCVAGIRVFSTGIVNYKQVCLKYVELIQQQGGDLRLNTKVLKISPSGNN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCC
HVLETNNGNFETRFVINCAGLHSDRIAKLGGVKPSAKIVPFRGEYYELTPEKRYLVKTLI
EEEECCCCCEEEEEEEEEECCCCHHHHHHCCCCCCCEEEECCCCEEEECCCHHHHHHHHC
YPVPNPEFPFLGVHFTRMIDGSVHAGPNAVLSLKREGYKKTDFDLRDFAEVMTYPGFWKL
CCCCCCCCCEEEEEEEEEECCCCCCCCHHEEEEHHCCCCCCCCCHHHHHHHHCCCCCEEC
AGKHADEGIQEIIRSFSKAAFTRSLQNLIPEVQAEDLVPTHAGVRAQALMDDGKLVDDFY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCEEEEECCCCCEEEEEE
IVPGENSIHVCNAPSPAATSSLEIGKAIAAQIPQQSHLENVVIA
EECCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]