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The map label for this gene is ispG [H]

Identifier: 17229993

GI number: 17229993

Start: 3001329

End: 3002555

Strand: Reverse

Name: ispG [H]

Synonym: all2501

Alternate gene names: 17229993

Gene position: 3002555-3001329 (Counterclockwise)

Preceding gene: 17229998

Following gene: 17229992

Centisome position: 46.81

GC content: 44.5

Gene sequence:

>1227_bases
ATGCAAACTCTGCCGACACCCACAACATCCAGTAATACAGCCAACCAAGCCACCTTTGATACTACAATCAAGCGTCGTAA
AACCCGTCCTGTGAAAGTGGGTAATGTCACCATCGGCGGTGGCTACCCTGTGGTGGTGCAGTCGATGATTAACGAAGACA
CTCTTGATATCGATGGTTCTGTAGCCGCTATTCGGCGTTTGCACGAAATTGGCTGTGAAATCGTCCGTGTCACAGTGCCG
AGCATAGCTCACGCCGTAGCGTTGGCAGAAATTAAACAAAAACTCATTAAAACTTATCAAGATGTGCCAATTGTTGCTGA
CGTACATCACAATGGAATGAAAATTGCCCTGGAAGTTGCCAAACATATAGAAAAAGTCCGGATAAATCCCGGCTTGTATG
TGTTTGAAAAACCCAACACCAATAGAACTGAATATACTCAAGCCGAATTTGAGGAAATTGGCGAAAAAATCCGCGAAACT
CTCGCACCCTTGGTAATTACCCTGCGCGACCAAGGTAAAGCCATGCGTATTGGTGTCAATCACGGTTCTCTCGCTGAGAG
AATGTTATTTACTTACGGCGACACTCCAGAAGGCATGGTGGAATCTGCCTTAGAATTTATTCGCATCTGCGAATCTCTCG
ACTTCCGTAACATAGTCATTTCCATGAAAGCTTCACGAGTTCCCGTGATGGTAGCCGCCTATCGCCTCATGGCTAAACGC
ATGGATGATTTAGGAATGGATTACCCCTTACATTTAGGTGTCACCGAAGCTGGTGATGGTGAATATGGACGGATTAAATC
CACAGCAGGTATTGCCACATTATTAGCTGATGGTATCGGCGATACTATTCGCGTCTCCCTGACAGAAGCACCGGAAAAGG
AAATTCCCGTCTGTTACAGCATTCTGCAAGCTTTAGGTTTGCGGAAAACAATGGTGGAATATGTAGCCTGTCCTTCCTGC
GGACGTACTTTATTTAACTTAGAAGAAGTATTACATAAAGTACGTGAATCTACTAAACACCTCACAGGACTAGACATTGC
TGTCATGGGTTGCATTGTCAATGGCCCAGGAGAGATGGCTGATGCTGACTACGGTTATGTAGGTAAAACTCCTGGTTACA
TTTCTTTATATCGTGGCAGAGAAGAAATTAAAAAAGTCCCAGAAGATAAAGGCGTTGAGGAACTAATTAACTTAATTAAA
GCTGATGGTCGTTGGGTAGATCCTTAG

Upstream 100 bases:

>100_bases
TCCATTTCATGGTTCAAAAAAGCTCCCACTGTGGCAATCTGAGAAACATAGGCTTTTAAATCTGATATTTTAACTACAGC
AAATCCACCTAATTAAGGTT

Downstream 100 bases:

>100_bases
AGGAAGCAGGGGAGCAGAGGGAAGTAATAACTCTCTACTCCCACATTGATTCATAAACTTTCTGTAAACAACAATCGGCT
AGGTCGCCGGATTACAAAGT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQTLPTPTTSSNTANQATFDTTIKRRKTRPVKVGNVTIGGGYPVVVQSMINEDTLDIDGSVAAIRRLHEIGCEIVRVTVP
SIAHAVALAEIKQKLIKTYQDVPIVADVHHNGMKIALEVAKHIEKVRINPGLYVFEKPNTNRTEYTQAEFEEIGEKIRET
LAPLVITLRDQGKAMRIGVNHGSLAERMLFTYGDTPEGMVESALEFIRICESLDFRNIVISMKASRVPVMVAAYRLMAKR
MDDLGMDYPLHLGVTEAGDGEYGRIKSTAGIATLLADGIGDTIRVSLTEAPEKEIPVCYSILQALGLRKTMVEYVACPSC
GRTLFNLEEVLHKVRESTKHLTGLDIAVMGCIVNGPGEMADADYGYVGKTPGYISLYRGREEIKKVPEDKGVEELINLIK
ADGRWVDP

Sequences:

>Translated_408_residues
MQTLPTPTTSSNTANQATFDTTIKRRKTRPVKVGNVTIGGGYPVVVQSMINEDTLDIDGSVAAIRRLHEIGCEIVRVTVP
SIAHAVALAEIKQKLIKTYQDVPIVADVHHNGMKIALEVAKHIEKVRINPGLYVFEKPNTNRTEYTQAEFEEIGEKIRET
LAPLVITLRDQGKAMRIGVNHGSLAERMLFTYGDTPEGMVESALEFIRICESLDFRNIVISMKASRVPVMVAAYRLMAKR
MDDLGMDYPLHLGVTEAGDGEYGRIKSTAGIATLLADGIGDTIRVSLTEAPEKEIPVCYSILQALGLRKTMVEYVACPSC
GRTLFNLEEVLHKVRESTKHLTGLDIAVMGCIVNGPGEMADADYGYVGKTPGYISLYRGREEIKKVPEDKGVEELINLIK
ADGRWVDP
>Mature_408_residues
MQTLPTPTTSSNTANQATFDTTIKRRKTRPVKVGNVTIGGGYPVVVQSMINEDTLDIDGSVAAIRRLHEIGCEIVRVTVP
SIAHAVALAEIKQKLIKTYQDVPIVADVHHNGMKIALEVAKHIEKVRINPGLYVFEKPNTNRTEYTQAEFEEIGEKIRET
LAPLVITLRDQGKAMRIGVNHGSLAERMLFTYGDTPEGMVESALEFIRICESLDFRNIVISMKASRVPVMVAAYRLMAKR
MDDLGMDYPLHLGVTEAGDGEYGRIKSTAGIATLLADGIGDTIRVSLTEAPEKEIPVCYSILQALGLRKTMVEYVACPSC
GRTLFNLEEVLHKVRESTKHLTGLDIAVMGCIVNGPGEMADADYGYVGKTPGYISLYRGREEIKKVPEDKGVEELINLIK
ADGRWVDP

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=349, Percent_Identity=38.9684813753582, Blast_Score=232, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44928; Mature: 44928

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTLPTPTTSSNTANQATFDTTIKRRKTRPVKVGNVTIGGGYPVVVQSMINEDTLDIDGS
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECEEEECCCHHHHHHHHHCCCCCCCCCH
VAAIRRLHEIGCEIVRVTVPSIAHAVALAEIKQKLIKTYQDVPIVADVHHNGMKIALEVA
HHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
KHIEKVRINPGLYVFEKPNTNRTEYTQAEFEEIGEKIRETLAPLVITLRDQGKAMRIGVN
HHHHHEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECC
HGSLAERMLFTYGDTPEGMVESALEFIRICESLDFRNIVISMKASRVPVMVAAYRLMAKR
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHH
MDDLGMDYPLHLGVTEAGDGEYGRIKSTAGIATLLADGIGDTIRVSLTEAPEKEIPVCYS
HHHCCCCCCEEECCEECCCCCCCCEEHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHH
ILQALGLRKTMVEYVACPSCGRTLFNLEEVLHKVRESTKHLTGLDIAVMGCIVNGPGEMA
HHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
DADYGYVGKTPGYISLYRGREEIKKVPEDKGVEELINLIKADGRWVDP
CCCCCCCCCCCCEEEEECCHHHHHCCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQTLPTPTTSSNTANQATFDTTIKRRKTRPVKVGNVTIGGGYPVVVQSMINEDTLDIDGS
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECEEEECCCHHHHHHHHHCCCCCCCCCH
VAAIRRLHEIGCEIVRVTVPSIAHAVALAEIKQKLIKTYQDVPIVADVHHNGMKIALEVA
HHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
KHIEKVRINPGLYVFEKPNTNRTEYTQAEFEEIGEKIRETLAPLVITLRDQGKAMRIGVN
HHHHHEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECC
HGSLAERMLFTYGDTPEGMVESALEFIRICESLDFRNIVISMKASRVPVMVAAYRLMAKR
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHH
MDDLGMDYPLHLGVTEAGDGEYGRIKSTAGIATLLADGIGDTIRVSLTEAPEKEIPVCYS
HHHCCCCCCEEECCEECCCCCCCCEEHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHH
ILQALGLRKTMVEYVACPSCGRTLFNLEEVLHKVRESTKHLTGLDIAVMGCIVNGPGEMA
HHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
DADYGYVGKTPGYISLYRGREEIKKVPEDKGVEELINLIKADGRWVDP
CCCCCCCCCCCCEEEEECCHHHHHCCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA