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The map label for this gene is pleD [H]

Identifier: 17229908

GI number: 17229908

Start: 2899124

End: 2900122

Strand: Reverse

Name: pleD [H]

Synonym: all2416

Alternate gene names: 17229908

Gene position: 2900122-2899124 (Counterclockwise)

Preceding gene: 17229909

Following gene: 17229907

Centisome position: 45.22

GC content: 43.54

Gene sequence:

>999_bases
ATGACTATGACAATTCTGGTCTTTGGAAGTTATGACTTTCTCACAAAACTTCCCGATCAGATCCATAAAAGAGCTACTGA
TAAGTTAGTAGTGATTACGGATTTTCATCAGGCCGCATCGCAGATCCAAACTGCATCACCCGATGTCATATTTGTACAGG
CAAGCCTCAAGGGTAGTTTAGAATTGTGCAGCTGGTTGAAAGAACAAACTCAGCTATCGTATATACACTGTATTCTCGTG
GAAGACCGTCCTCAACAACTAGCACAGCGAAGTCAAAACGGCTGGGAATGGGAGTTTGAGATGACTGCCACAGCACTTCA
CCAAGGTGCAGATGCTTATATTTGGCAACTGCCGACGGAAGTAATGGCAACTCACAATCTGATGTTAGGTCAATTCACAG
TTGGTTTGCGTAAAGCGCAGAAGTACCGCGATTTGCTGCAAAAAAACCATATCTTATCAGCGATCGCCTTAGCTGATTCT
TTGACAGAATTGAACAATCGCCGCGCGTTGGAATGGGATTTACCCAGACAGATTACCAAAGCTCGTAGTCAAAATCTTCC
TTTGAGTTTGGTGATTCTAGATGTGGATTATTTTAAGAAAGTCAATGATCAGTATGGACACTTAATTGGCGATCGCCTGT
TGCAGTCGTTGTGTACTCGGCTACGCCATAATTTACGCTCTCAAGATACGCCGTTCCGTTATGGTGGTGAGGAGTTTGTC
GTTATTCTGGCTAATACCACTGGTGAAGAGGCAATGATCGTGGCGGATCGCCTCAATCGGATAGTAGGTGAACAGCAGTT
TAGTATCAATAGTAAACTCATGATTAGTGTCACTATTAGCTTGGGTGTAGCCTGTCTGGAAACAGATGATGATGAACAGG
GAAATAGCCTGTTACATCGCGCTGATCAATGCCTACTAGCAGCTAAAGCATCTGGACGTAACCAAGTTATCGGGGAGCAT
CAATTATCTAAATTTTCATCTCTAGAAGCCGTTTCTTGA

Upstream 100 bases:

>100_bases
ATCAGTATATGTAAGGGTCTCTTGTTTCCAGAGACAATATATTCATGAATCGGCAGAATATCAGTAATTATTAGCTAGAA
AAAAGGCAAAAAATACAGCC

Downstream 100 bases:

>100_bases
TAGTGTTCTGCCTACCGCTACGTTTAAAAAACGCACTGATGATATGTGAAGCTGTAGCGTAAATTAGTTGGCTGTCAATG
TCAACAAGTAGTACTTTTTA

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTMTILVFGSYDFLTKLPDQIHKRATDKLVVITDFHQAASQIQTASPDVIFVQASLKGSLELCSWLKEQTQLSYIHCILV
EDRPQQLAQRSQNGWEWEFEMTATALHQGADAYIWQLPTEVMATHNLMLGQFTVGLRKAQKYRDLLQKNHILSAIALADS
LTELNNRRALEWDLPRQITKARSQNLPLSLVILDVDYFKKVNDQYGHLIGDRLLQSLCTRLRHNLRSQDTPFRYGGEEFV
VILANTTGEEAMIVADRLNRIVGEQQFSINSKLMISVTISLGVACLETDDDEQGNSLLHRADQCLLAAKASGRNQVIGEH
QLSKFSSLEAVS

Sequences:

>Translated_332_residues
MTMTILVFGSYDFLTKLPDQIHKRATDKLVVITDFHQAASQIQTASPDVIFVQASLKGSLELCSWLKEQTQLSYIHCILV
EDRPQQLAQRSQNGWEWEFEMTATALHQGADAYIWQLPTEVMATHNLMLGQFTVGLRKAQKYRDLLQKNHILSAIALADS
LTELNNRRALEWDLPRQITKARSQNLPLSLVILDVDYFKKVNDQYGHLIGDRLLQSLCTRLRHNLRSQDTPFRYGGEEFV
VILANTTGEEAMIVADRLNRIVGEQQFSINSKLMISVTISLGVACLETDDDEQGNSLLHRADQCLLAAKASGRNQVIGEH
QLSKFSSLEAVS
>Mature_331_residues
TMTILVFGSYDFLTKLPDQIHKRATDKLVVITDFHQAASQIQTASPDVIFVQASLKGSLELCSWLKEQTQLSYIHCILVE
DRPQQLAQRSQNGWEWEFEMTATALHQGADAYIWQLPTEVMATHNLMLGQFTVGLRKAQKYRDLLQKNHILSAIALADSL
TELNNRRALEWDLPRQITKARSQNLPLSLVILDVDYFKKVNDQYGHLIGDRLLQSLCTRLRHNLRSQDTPFRYGGEEFVV
ILANTTGEEAMIVADRLNRIVGEQQFSINSKLMISVTISLGVACLETDDDEQGNSLLHRADQCLLAAKASGRNQVIGEHQ
LSKFSSLEAVS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=36.7088607594937, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87082007, Length=164, Percent_Identity=37.8048780487805, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=35.8823529411765, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787262, Length=179, Percent_Identity=35.195530726257, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786584, Length=217, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788381, Length=165, Percent_Identity=33.9393939393939, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=36.046511627907, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787802, Length=174, Percent_Identity=31.6091954022989, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787541, Length=167, Percent_Identity=33.5329341317365, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788085, Length=212, Percent_Identity=26.8867924528302, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787056, Length=162, Percent_Identity=32.0987654320988, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87081977, Length=184, Percent_Identity=28.804347826087, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87081974, Length=104, Percent_Identity=28.8461538461538, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 37482; Mature: 37351

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMTILVFGSYDFLTKLPDQIHKRATDKLVVITDFHQAASQIQTASPDVIFVQASLKGSL
CEEEEEEEECHHHHHHCHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCEEEEEECCCCCH
ELCSWLKEQTQLSYIHCILVEDRPQQLAQRSQNGWEWEFEMTATALHQGADAYIWQLPTE
HHHHHHHHHCCEEEEEEEEECCCHHHHHHHCCCCCEEEEEEHHHHHHCCCCEEEEECCHH
VMATHNLMLGQFTVGLRKAQKYRDLLQKNHILSAIALADSLTELNNRRALEWDLPRQITK
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHH
ARSQNLPLSLVILDVDYFKKVNDQYGHLIGDRLLQSLCTRLRHNLRSQDTPFRYGGEEFV
HHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCEEE
VILANTTGEEAMIVADRLNRIVGEQQFSINSKLMISVTISLGVACLETDDDEQGNSLLHR
EEEECCCCCCHHHHHHHHHHHHCCHHCCCCCEEEEEEEEECCEEEEECCCCCCHHHHHHH
ADQCLLAAKASGRNQVIGEHQLSKFSSLEAVS
HHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
TMTILVFGSYDFLTKLPDQIHKRATDKLVVITDFHQAASQIQTASPDVIFVQASLKGSL
EEEEEEEECHHHHHHCHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCEEEEEECCCCCH
ELCSWLKEQTQLSYIHCILVEDRPQQLAQRSQNGWEWEFEMTATALHQGADAYIWQLPTE
HHHHHHHHHCCEEEEEEEEECCCHHHHHHHCCCCCEEEEEEHHHHHHCCCCEEEEECCHH
VMATHNLMLGQFTVGLRKAQKYRDLLQKNHILSAIALADSLTELNNRRALEWDLPRQITK
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHH
ARSQNLPLSLVILDVDYFKKVNDQYGHLIGDRLLQSLCTRLRHNLRSQDTPFRYGGEEFV
HHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCEEE
VILANTTGEEAMIVADRLNRIVGEQQFSINSKLMISVTISLGVACLETDDDEQGNSLLHR
EEEECCCCCCHHHHHHHHHHHHCCHHCCCCCEEEEEEEEECCEEEEECCCCCCHHHHHHH
ADQCLLAAKASGRNQVIGEHQLSKFSSLEAVS
HHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA