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The map label for this gene is bioB [H]

Identifier: 17229413

GI number: 17229413

Start: 2303806

End: 2304813

Strand: Direct

Name: bioB [H]

Synonym: alr1921

Alternate gene names: 17229413

Gene position: 2303806-2304813 (Clockwise)

Preceding gene: 17229412

Following gene: 17229415

Centisome position: 35.92

GC content: 44.44

Gene sequence:

>1008_bases
ATGTCGGTGGGAAGAATACGCTACGATTGGCACAAAGCAGAGATTCGCGCAGTATATGATACGCCATTGCTAGAGTTGAT
TTATCAAGCTGCTAGTGTACATCGGCAGTTTCATAATCCTAAACAAATACAAGTATGTAAGCTGATATCGATTAAAACAG
GTGCGTGTCCTGAAGATTGTAGTTACTGCGCCCAGTCTTCTCGTTATCAAACTGAAGTCAAACCCCAAGCACTCCTTGAT
AAGCAAACGGTTGTGGAAATTGCCCAAAACGCTAAACAAAAGGGTGTCAGCCGTGTCTGTATGGGTGCGGCTTGGCGGGA
GGTGCGAGATAATTCTCAATTTGACCGAGTTCTAGAAATGGTCAAAGATGTTACAGACATGGGTTTAGAAGTGTGCTGTA
CTCTGGGAATGCTGACATCTGAGCAAGCCAAGAAGTTGGAGACAGCAGGATTGTATGCTTATAACCATAACTTGGATACT
TCCTCTGACTACTACAGCACTATTATTACCACGAGAACTTATGGCGATCGCCTAAACACTATCGAAAACGTCCGACAGAC
TAATGTTACCGTATGTTCTGGCGGCATTCTCGGTTTAGGTGAAAGCATAGATGACCGTGTGGCAATGTTACAAACCTTAG
CCACCCTCAATCCTCATCCAGAATCCGTACCAATTAATATTCTCTCCCAAGTAGAAGGCACACCTCTAGAAGATCAACCA
GATGTACCTGTTTGGGATGTAGTCAGAATGATTGCAACTGCACGTATAGTTATGCCCACATCGGATGTGCGTTTAAGTGC
TGGTAGGGCGAGACTTTCTCAAGTGGAACAAGCCTTTTGTTTTATGGCAGGAGCAAATTCCATCTTTTCCAGCGACGATA
ATAAAATGCTGACGGTGACAACACCTTGTCCAGATTACGATGCTGATCAGGAAATGCTCAACCTACTCGGCTTAGAAATG
CGTCCTCCGTCCCAAAGACCACAACCTGTAGTCATGGGTAATTCATAA

Upstream 100 bases:

>100_bases
ATGAGAAAGAAACATCTCAAAACTCTTGCTGTCTCCCCCAATCCCCAGTCCCCAGTCCCCAATCCCCAGTCCCTAATACT
GAAATAAGGATACTATACTG

Downstream 100 bases:

>100_bases
TCGCTTCAGATTCCCGACTTCTTGAAGAAGTTGGGAATCTAGTTTTTCACACTACAGTATCTTCGCAGAGCGTAAACCAA
ACAGCAGACCAGCAGCTATG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSVGRIRYDWHKAEIRAVYDTPLLELIYQAASVHRQFHNPKQIQVCKLISIKTGACPEDCSYCAQSSRYQTEVKPQALLD
KQTVVEIAQNAKQKGVSRVCMGAAWREVRDNSQFDRVLEMVKDVTDMGLEVCCTLGMLTSEQAKKLETAGLYAYNHNLDT
SSDYYSTIITTRTYGDRLNTIENVRQTNVTVCSGGILGLGESIDDRVAMLQTLATLNPHPESVPINILSQVEGTPLEDQP
DVPVWDVVRMIATARIVMPTSDVRLSAGRARLSQVEQAFCFMAGANSIFSSDDNKMLTVTTPCPDYDADQEMLNLLGLEM
RPPSQRPQPVVMGNS

Sequences:

>Translated_335_residues
MSVGRIRYDWHKAEIRAVYDTPLLELIYQAASVHRQFHNPKQIQVCKLISIKTGACPEDCSYCAQSSRYQTEVKPQALLD
KQTVVEIAQNAKQKGVSRVCMGAAWREVRDNSQFDRVLEMVKDVTDMGLEVCCTLGMLTSEQAKKLETAGLYAYNHNLDT
SSDYYSTIITTRTYGDRLNTIENVRQTNVTVCSGGILGLGESIDDRVAMLQTLATLNPHPESVPINILSQVEGTPLEDQP
DVPVWDVVRMIATARIVMPTSDVRLSAGRARLSQVEQAFCFMAGANSIFSSDDNKMLTVTTPCPDYDADQEMLNLLGLEM
RPPSQRPQPVVMGNS
>Mature_334_residues
SVGRIRYDWHKAEIRAVYDTPLLELIYQAASVHRQFHNPKQIQVCKLISIKTGACPEDCSYCAQSSRYQTEVKPQALLDK
QTVVEIAQNAKQKGVSRVCMGAAWREVRDNSQFDRVLEMVKDVTDMGLEVCCTLGMLTSEQAKKLETAGLYAYNHNLDTS
SDYYSTIITTRTYGDRLNTIENVRQTNVTVCSGGILGLGESIDDRVAMLQTLATLNPHPESVPINILSQVEGTPLEDQPD
VPVWDVVRMIATARIVMPTSDVRLSAGRARLSQVEQAFCFMAGANSIFSSDDNKMLTVTTPCPDYDADQEMLNLLGLEMR
PPSQRPQPVVMGNS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=312, Percent_Identity=51.9230769230769, Blast_Score=330, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6321725, Length=326, Percent_Identity=48.159509202454, Blast_Score=331, Evalue=8e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37246; Mature: 37115

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVGRIRYDWHKAEIRAVYDTPLLELIYQAASVHRQFHNPKQIQVCKLISIKTGACPEDC
CCCCCEEEEEHHHHEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHH
SYCAQSSRYQTEVKPQALLDKQTVVEIAQNAKQKGVSRVCMGAAWREVRDNSQFDRVLEM
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VKDVTDMGLEVCCTLGMLTSEQAKKLETAGLYAYNHNLDTSSDYYSTIITTRTYGDRLNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHEEEECCCCHHHHH
IENVRQTNVTVCSGGILGLGESIDDRVAMLQTLATLNPHPESVPINILSQVEGTPLEDQP
HHHHHHCCEEEECCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCC
DVPVWDVVRMIATARIVMPTSDVRLSAGRARLSQVEQAFCFMAGANSIFSSDDNKMLTVT
CCCHHHHHHHHHHHHEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEE
TPCPDYDADQEMLNLLGLEMRPPSQRPQPVVMGNS
CCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCC
>Mature Secondary Structure 
SVGRIRYDWHKAEIRAVYDTPLLELIYQAASVHRQFHNPKQIQVCKLISIKTGACPEDC
CCCCEEEEEHHHHEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHH
SYCAQSSRYQTEVKPQALLDKQTVVEIAQNAKQKGVSRVCMGAAWREVRDNSQFDRVLEM
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VKDVTDMGLEVCCTLGMLTSEQAKKLETAGLYAYNHNLDTSSDYYSTIITTRTYGDRLNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHEEEECCCCHHHHH
IENVRQTNVTVCSGGILGLGESIDDRVAMLQTLATLNPHPESVPINILSQVEGTPLEDQP
HHHHHHCCEEEECCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCC
DVPVWDVVRMIATARIVMPTSDVRLSAGRARLSQVEQAFCFMAGANSIFSSDDNKMLTVT
CCCHHHHHHHHHHHHEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEE
TPCPDYDADQEMLNLLGLEMRPPSQRPQPVVMGNS
CCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA