Click here to switch to the map view.

The map label for this gene is lacF [H]

Identifier: 17228284

GI number: 17228284

Start: 911188

End: 912072

Strand: Direct

Name: lacF [H]

Synonym: alr0789

Alternate gene names: 17228284

Gene position: 911188-912072 (Clockwise)

Preceding gene: 17228282

Following gene: 17228285

Centisome position: 14.21

GC content: 43.28

Gene sequence:

>885_bases
ATGCCGAAAATATCCACTAAGTCATGGTTAGACAATGATACATTCGCCGCTTGGGCTTTTCTCGCCCCGGCTGTAATTTT
GTTGGGTTTATTTGTCCTGTGGCCTATTGCCTATTTGTTTTATCTAAGTTTTACTGCTGGCAGTTTCACTTCCACAGGTA
CATATTGGGTAGGTTTCAAGAATTACTGGCGATTGTTACTTAACCCTGATTTTTGGCAAGTTATTGGTAACACCGTTTAT
TTTACGGTAGCTTCTGTAATCCCCAGCTTAGTGATTCCTTTGGGGCTAGCAGTTTTATTGAACCGTTCTATGGCCTTGCG
GGGGGTGCTACGTAGTGCATATTTTCTACCATCAATTATTTCTCTGGTTGCTGCTGGGTTAGGCTTTCGTTGGTTGTTTC
AAACCTCTGGCCCGGTAAACGCATTTTTAAATATATTTGGCATCCCAGCAATTTCTTGGTTAGGCGATACCTTTTGGGCA
ATGCCTGTACTAATCATTTTAAGCATTTGGAAACAACTAGGGTTTAATATGGTGGTGTTCTTGGCAGGGTTGCAAGCCAT
TCCCCCCAGTCGCTACGAAGCCGCAGAATTAGATGGTGCCAATGGTTGGCGACAGTTTTGGCATATCACCCTACCAGGAT
TAAGACCCACTTTAATCTTTGCCACTGTCACCACGGCTATTTTTACATTGCGGAGTTTTGAGCAAGTTTATGTAATGACT
GGTGGAGGCCCTTTAAATACAACTAACTTACTGGTTTACTACATCTATCAGGAAGCTTTCGGACAATTTGATTTTGGTTA
CGCCGCCGCCGCCGCAACGGTACTATTAGCTATGGCTTTGGTTCTTGTGTATTTGCAATTGCGTACTTGGGAAGAGGAAG
CATAA

Upstream 100 bases:

>100_bases
AGAATATACACTCAGCACTTTCAAGTTAATTACTCATGAGAAAATTTCTTTTACCTACACCCTTACACCCATTTTCAAGA
CAGTTGAGAGGAAAATTATA

Downstream 100 bases:

>100_bases
GTTGGGTAATGGGTAATGGATGATTGGTAATTGGTAATTGGAGTATTTTATAGTCCATAACCTCTGATTTCCTAGAAAAT
GTAGTAAATTTTACGTCTAC

Product: ABC transporter sugar permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MPKISTKSWLDNDTFAAWAFLAPAVILLGLFVLWPIAYLFYLSFTAGSFTSTGTYWVGFKNYWRLLLNPDFWQVIGNTVY
FTVASVIPSLVIPLGLAVLLNRSMALRGVLRSAYFLPSIISLVAAGLGFRWLFQTSGPVNAFLNIFGIPAISWLGDTFWA
MPVLIILSIWKQLGFNMVVFLAGLQAIPPSRYEAAELDGANGWRQFWHITLPGLRPTLIFATVTTAIFTLRSFEQVYVMT
GGGPLNTTNLLVYYIYQEAFGQFDFGYAAAAATVLLAMALVLVYLQLRTWEEEA

Sequences:

>Translated_294_residues
MPKISTKSWLDNDTFAAWAFLAPAVILLGLFVLWPIAYLFYLSFTAGSFTSTGTYWVGFKNYWRLLLNPDFWQVIGNTVY
FTVASVIPSLVIPLGLAVLLNRSMALRGVLRSAYFLPSIISLVAAGLGFRWLFQTSGPVNAFLNIFGIPAISWLGDTFWA
MPVLIILSIWKQLGFNMVVFLAGLQAIPPSRYEAAELDGANGWRQFWHITLPGLRPTLIFATVTTAIFTLRSFEQVYVMT
GGGPLNTTNLLVYYIYQEAFGQFDFGYAAAAATVLLAMALVLVYLQLRTWEEEA
>Mature_293_residues
PKISTKSWLDNDTFAAWAFLAPAVILLGLFVLWPIAYLFYLSFTAGSFTSTGTYWVGFKNYWRLLLNPDFWQVIGNTVYF
TVASVIPSLVIPLGLAVLLNRSMALRGVLRSAYFLPSIISLVAAGLGFRWLFQTSGPVNAFLNIFGIPAISWLGDTFWAM
PVLIILSIWKQLGFNMVVFLAGLQAIPPSRYEAAELDGANGWRQFWHITLPGLRPTLIFATVTTAIFTLRSFEQVYVMTG
GGPLNTTNLLVYYIYQEAFGQFDFGYAAAAATVLLAMALVLVYLQLRTWEEEA

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787570, Length=265, Percent_Identity=28.6792452830189, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1789861, Length=277, Percent_Identity=28.158844765343, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1790465, Length=258, Percent_Identity=34.4961240310078, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32794; Mature: 32663

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKISTKSWLDNDTFAAWAFLAPAVILLGLFVLWPIAYLFYLSFTAGSFTSTGTYWVGFK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHH
NYWRLLLNPDFWQVIGNTVYFTVASVIPSLVIPLGLAVLLNRSMALRGVLRSAYFLPSII
HHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLVAAGLGFRWLFQTSGPVNAFLNIFGIPAISWLGDTFWAMPVLIILSIWKQLGFNMVVF
HHHHHCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LAGLQAIPPSRYEAAELDGANGWRQFWHITLPGLRPTLIFATVTTAIFTLRSFEQVYVMT
HHHHHCCCCCCCCHHHCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
GGGPLNTTNLLVYYIYQEAFGQFDFGYAAAAATVLLAMALVLVYLQLRTWEEEA
CCCCCCHHHHHHHHEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PKISTKSWLDNDTFAAWAFLAPAVILLGLFVLWPIAYLFYLSFTAGSFTSTGTYWVGFK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHH
NYWRLLLNPDFWQVIGNTVYFTVASVIPSLVIPLGLAVLLNRSMALRGVLRSAYFLPSII
HHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLVAAGLGFRWLFQTSGPVNAFLNIFGIPAISWLGDTFWAMPVLIILSIWKQLGFNMVVF
HHHHHCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LAGLQAIPPSRYEAAELDGANGWRQFWHITLPGLRPTLIFATVTTAIFTLRSFEQVYVMT
HHHHHCCCCCCCCHHHCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
GGGPLNTTNLLVYYIYQEAFGQFDFGYAAAAATVLLAMALVLVYLQLRTWEEEA
CCCCCCHHHHHHHHEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]