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The map label for this gene is hupB [H]

Identifier: 17228195

GI number: 17228195

Start: 806344

End: 807189

Strand: Direct

Name: hupB [H]

Synonym: alr0700

Alternate gene names: 17228195

Gene position: 806344-807189 (Clockwise)

Preceding gene: 17228194

Following gene: 17228196

Centisome position: 12.57

GC content: 38.18

Gene sequence:

>846_bases
ATGTGTGTAACCTGTGGTTGTTCTGATGAGTCTGAAGATAAAATTACTAATTTAGAAACAGGCGAAATGGTACATAATCA
CCATCATCATACTCACACCTTAGCTGATGGTACTGTCATTACTCACTCTCATAGTCATGAGCAGGAACCATCACAAATTC
ATGCCAAAATTCATAACACTACGATATCTTTAGAACAAGAAATTTTAGGGAAAAATAACCTATTAGCTGCCCAAAATCGG
GGATGGTTTAAAGGTCGAAATATTCTGTCCTTAAATCTAATGAGTTCTCCTGGCGCAGGTAAAACAACTCTTTTGACTCA
GACTATCAACGACCTGAAGCATCAATTACCCATAACTGTGATTGAAGGCGACCAAGAAACAATTAACGATGCCGAAAAAA
TTAAAGAAACTGGCTGTAAAGTAGTCCAAATTAACACGGGAACAGGTTGTCATTTGGATGCTTCCATGATTGAAAGGGGT
TTACAACAATTAAACCCACCGATAAACTCTGTTTTGATGATTGAAAATGTCGGTAATTTAGTTTGTCCGGCTTTATTTGA
TTTGGGTGAACAAGCGAAAGTCGTGATTCTTTCGGTGACAGAGGGAGAGGATAAACCAATCAAATACCCCCACATCTTCC
GCGCCAGTGAGATTATGATTCTCACTAAAATTGATTTGTTGCCCTATGTGAATTTCGATGTGCAGCGTTGTGTAGAATAT
GCAAAGCAGGTAAACCCGAACATCCAGATTTTTCAAGTTTCTGCAACTACGGGTTTAGGGTTGGATACTTGGTATAAATG
GTTAACGAATAAAGTAGCTAATTCATCAGTACCAGTTTTTCCTTAA

Upstream 100 bases:

>100_bases
CTTTAGAAAAACCATTTGGTATTTGTGGTTGTGGTAGTGTGCATTTAGATGTAATTACTGGTGAAGAACTGAAAATCAAA
GAAATAGAGGTCGAAGAAGT

Downstream 100 bases:

>100_bases
TCTAGTAATTTTGCGTCAACCTGTAGATGTTTTTTAGCCACGATGAACATTTCTGACTGAAAAGCTTATACTCTATGTCT
TTGGACGGTTTTTCCTCAAA

Product: hydrogenase expression/formation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MCVTCGCSDESEDKITNLETGEMVHNHHHHTHTLADGTVITHSHSHEQEPSQIHAKIHNTTISLEQEILGKNNLLAAQNR
GWFKGRNILSLNLMSSPGAGKTTLLTQTINDLKHQLPITVIEGDQETINDAEKIKETGCKVVQINTGTGCHLDASMIERG
LQQLNPPINSVLMIENVGNLVCPALFDLGEQAKVVILSVTEGEDKPIKYPHIFRASEIMILTKIDLLPYVNFDVQRCVEY
AKQVNPNIQIFQVSATTGLGLDTWYKWLTNKVANSSVPVFP

Sequences:

>Translated_281_residues
MCVTCGCSDESEDKITNLETGEMVHNHHHHTHTLADGTVITHSHSHEQEPSQIHAKIHNTTISLEQEILGKNNLLAAQNR
GWFKGRNILSLNLMSSPGAGKTTLLTQTINDLKHQLPITVIEGDQETINDAEKIKETGCKVVQINTGTGCHLDASMIERG
LQQLNPPINSVLMIENVGNLVCPALFDLGEQAKVVILSVTEGEDKPIKYPHIFRASEIMILTKIDLLPYVNFDVQRCVEY
AKQVNPNIQIFQVSATTGLGLDTWYKWLTNKVANSSVPVFP
>Mature_281_residues
MCVTCGCSDESEDKITNLETGEMVHNHHHHTHTLADGTVITHSHSHEQEPSQIHAKIHNTTISLEQEILGKNNLLAAQNR
GWFKGRNILSLNLMSSPGAGKTTLLTQTINDLKHQLPITVIEGDQETINDAEKIKETGCKVVQINTGTGCHLDASMIERG
LQQLNPPINSVLMIENVGNLVCPALFDLGEQAKVVILSVTEGEDKPIKYPHIFRASEIMILTKIDLLPYVNFDVQRCVEY
AKQVNPNIQIFQVSATTGLGLDTWYKWLTNKVANSSVPVFP

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=290, Percent_Identity=48.2758620689655, Blast_Score=266, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 31102; Mature: 31102

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVTCGCSDESEDKITNLETGEMVHNHHHHTHTLADGTVITHSHSHEQEPSQIHAKIHNT
CEEECCCCCCCCCHHCCCCCCHHHHCCCCCEEEECCCEEEECCCCCCCCHHHHEEEEECE
TISLEQEILGKNNLLAAQNRGWFKGRNILSLNLMSSPGAGKTTLLTQTINDLKHQLPITV
EEEEHHHHCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHCCEEE
IEGDQETINDAEKIKETGCKVVQINTGTGCHLDASMIERGLQQLNPPINSVLMIENVGNL
EECCHHHHHHHHHHHHCCCEEEEEECCCCCEECHHHHHHHHHHHCCCCCEEEEEECCCCH
VCPALFDLGEQAKVVILSVTEGEDKPIKYPHIFRASEIMILTKIDLLPYVNFDVQRCVEY
HHHHHHCCCCCCEEEEEEEECCCCCCCCCCCEECCCCEEEEEEECCCCCCCCCHHHHHHH
AKQVNPNIQIFQVSATTGLGLDTWYKWLTNKVANSSVPVFP
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MCVTCGCSDESEDKITNLETGEMVHNHHHHTHTLADGTVITHSHSHEQEPSQIHAKIHNT
CEEECCCCCCCCCHHCCCCCCHHHHCCCCCEEEECCCEEEECCCCCCCCHHHHEEEEECE
TISLEQEILGKNNLLAAQNRGWFKGRNILSLNLMSSPGAGKTTLLTQTINDLKHQLPITV
EEEEHHHHCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHCCEEE
IEGDQETINDAEKIKETGCKVVQINTGTGCHLDASMIERGLQQLNPPINSVLMIENVGNL
EECCHHHHHHHHHHHHCCCEEEEEECCCCCEECHHHHHHHHHHHCCCCCEEEEEECCCCH
VCPALFDLGEQAKVVILSVTEGEDKPIKYPHIFRASEIMILTKIDLLPYVNFDVQRCVEY
HHHHHHCCCCCCEEEEEEEECCCCCCCCCCCEECCCCEEEEEEECCCCCCCCCHHHHHHH
AKQVNPNIQIFQVSATTGLGLDTWYKWLTNKVANSSVPVFP
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]