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The map label for this gene is rluE [H]

Identifier: 17228041

GI number: 17228041

Start: 637215

End: 637829

Strand: Direct

Name: rluE [H]

Synonym: alr0545

Alternate gene names: 17228041

Gene position: 637215-637829 (Clockwise)

Preceding gene: 17228039

Following gene: 17228042

Centisome position: 9.94

GC content: 45.37

Gene sequence:

>615_bases
ATGACTAATGACGATCGCTATATTATTTTTCATAAACCCTACGGTGTCCTGAGCCAGTTTACTCAGGAATCGCCTAAGCA
TATAACTCTTAAAGATTATATTGATGTACCCGATGTTTATCCTGTGGGGAGATTGGATTGGGATAGTGAAGGGTTACTAT
TATTAACCAATAATGGCAGGTTGCAACATCGGCTTTCTGATCCGCGTTTTGGTCATGAGCGGACTTACTGGGTACAGGTA
GAGCGTATTCCTGATGAGGAGGCTATCAGCAAGCTACAAACAGGGGTGGAGATTCGAGATTATCGCACGCGGCCAGCAAA
AGTTGGATTATTACCAGAATATCCGCCTGTGGGCGATCGCCAACCACCTATAAGATTTCGCAAAAATGTTCCCACTGCTT
GGTTAGAAATGACTTTGACTGAAGGGAAAAATCGTCAAGTCCGGCGCATGACTGCGGCTGTGGGGTTTCCGACTTTGCGG
TTAGTACGGGTCAGTATAGGTCATTTACGCTTGGATGGTCTGGCTTTAGGTGAGTGGCGTGATCTCACTTCCTTGGAACT
TAAGTTACTGCATGATTCGTCTCAGAGTCGAGGGATAGGCAAATTTCCTAGTTAA

Upstream 100 bases:

>100_bases
CGAATTAGAGTAAAGAAAAGAGAAAATTTATATTCATGCTGAAAGCGATCGCCTTCTTTCTACAATTGATCAGGATCAAT
TGACCAATGACCAATGACCA

Downstream 100 bases:

>100_bases
ACTTTGTAAACAGTTGCAGGAATAAGCTAGTAATCATTTGAAGTTGAACAAAAGGCACAAGACTTTTTATTTGGCAAAAA
AAGAATTTAAAATTTGTTGT

Product: hypothetical protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MTNDDRYIIFHKPYGVLSQFTQESPKHITLKDYIDVPDVYPVGRLDWDSEGLLLLTNNGRLQHRLSDPRFGHERTYWVQV
ERIPDEEAISKLQTGVEIRDYRTRPAKVGLLPEYPPVGDRQPPIRFRKNVPTAWLEMTLTEGKNRQVRRMTAAVGFPTLR
LVRVSIGHLRLDGLALGEWRDLTSLELKLLHDSSQSRGIGKFPS

Sequences:

>Translated_204_residues
MTNDDRYIIFHKPYGVLSQFTQESPKHITLKDYIDVPDVYPVGRLDWDSEGLLLLTNNGRLQHRLSDPRFGHERTYWVQV
ERIPDEEAISKLQTGVEIRDYRTRPAKVGLLPEYPPVGDRQPPIRFRKNVPTAWLEMTLTEGKNRQVRRMTAAVGFPTLR
LVRVSIGHLRLDGLALGEWRDLTSLELKLLHDSSQSRGIGKFPS
>Mature_203_residues
TNDDRYIIFHKPYGVLSQFTQESPKHITLKDYIDVPDVYPVGRLDWDSEGLLLLTNNGRLQHRLSDPRFGHERTYWVQVE
RIPDEEAISKLQTGVEIRDYRTRPAKVGLLPEYPPVGDRQPPIRFRKNVPTAWLEMTLTEGKNRQVRRMTAAVGFPTLRL
VRVSIGHLRLDGLALGEWRDLTSLELKLLHDSSQSRGIGKFPS

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=55.6179775280899, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1790453, Length=193, Percent_Identity=34.1968911917098, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788510, Length=188, Percent_Identity=32.4468085106383, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787524, Length=153, Percent_Identity=35.9477124183007, Blast_Score=71, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 23554; Mature: 23423

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDDRYIIFHKPYGVLSQFTQESPKHITLKDYIDVPDVYPVGRLDWDSEGLLLLTNNGR
CCCCCCEEEEECCHHHHHHHHHCCCCEEEEHHCCCCCCCCCCCCCCCCCCCEEEEECCCE
LQHRLSDPRFGHERTYWVQVERIPDEEAISKLQTGVEIRDYRTRPAKVGLLPEYPPVGDR
EEEECCCCCCCCCEEEEEEEEECCCHHHHHHHHCCCEEEECCCCCEEECCCCCCCCCCCC
QPPIRFRKNVPTAWLEMTLTEGKNRQVRRMTAAVGFPTLRLVRVSIGHLRLDGLALGEWR
CCCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECEEEECCEEECCCC
DLTSLELKLLHDSSQSRGIGKFPS
CCCEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TNDDRYIIFHKPYGVLSQFTQESPKHITLKDYIDVPDVYPVGRLDWDSEGLLLLTNNGR
CCCCCEEEEECCHHHHHHHHHCCCCEEEEHHCCCCCCCCCCCCCCCCCCCEEEEECCCE
LQHRLSDPRFGHERTYWVQVERIPDEEAISKLQTGVEIRDYRTRPAKVGLLPEYPPVGDR
EEEECCCCCCCCCEEEEEEEEECCCHHHHHHHHCCCEEEECCCCCEEECCCCCCCCCCCC
QPPIRFRKNVPTAWLEMTLTEGKNRQVRRMTAAVGFPTLRLVRVSIGHLRLDGLALGEWR
CCCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECEEEECCEEECCCC
DLTSLELKLLHDSSQSRGIGKFPS
CCCEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]