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The map label for this gene is folD [H]

Identifier: 17227708

GI number: 17227708

Start: 228735

End: 229634

Strand: Direct

Name: folD [H]

Synonym: alr0212

Alternate gene names: 17227708

Gene position: 228735-229634 (Clockwise)

Preceding gene: 17227704

Following gene: 17227709

Centisome position: 3.57

GC content: 45.78

Gene sequence:

>900_bases
ATGGAAACAAAAACTGCCAAAATTCTTGATGGTAAAACTTTAGCTGAGAAAATTCAAAAAGAACTGACTGCTCAAATCAT
AGACGCACAAGCTAAAATTGGTCGTCCTCCTGGGTTAGCAGTATTGATGGTTGGAGATAACCCAGCCTCAGCAGCCTATG
TGCGTAACAAGGAAAAATCCTGCGCCAAAGTGGGTATTGCTTCTTTTGGCAAGCATTTTCCCCAAGAAACCACCCAAACA
GAATTAGAGGATGTGATTGCTGCACTCAACCAAGACGAACAGGTAGATGGCATTCTCGTACAATTGCCCTTACCTGAACA
TTTAGATGCGGTGAAACTGCTGCATCAAATAGAACCGGATAAAGATGCTGATGGACTCCACCCAGTTAATTTAGGGCGGT
TGGTGCGTGGGGAAAAGGGTTTACGCAGTTGCACCCCTGCTGGAGTCATGCGCCTATTGGCAGAATATGAAATTTCTTTG
CGAGGAAAGCAAGCTGTGGTGGTGGGACGGAGTATTTTGGTAGGGAAGCCAATGGCTTTGATGCTCCTAGAAGCTGATGC
CACCGTGACTATTGCCCACTCGCGATCGCAAGACCTAAAATCCATCACTCAAAATGCCGACATTCTCATTGCTGCTGCCG
GGTTGCCAGGATTAATTACTGCTGACATGGTGAAACCAGGCGCTGTTGTGGTAGATGTGGGGATAAATCGCGTGAGTGAT
GCTCATGGCAAAAGTCGCTTAGTAGGCGATATTAATTTTGCATCAATTGCTGGTGTGGCAGAATATATCACCCCGGTTCC
CGGTGGTATTGGCCCTATGACTGTGGCTTTGTTATTACAAAATACAGTCACCAGCTATTTACAAACAGCAAAAGAAAGCG
GAGCCTTGGATGTTAAATGA

Upstream 100 bases:

>100_bases
GTGGTAAAAAAGACGCTACGGCATCAGATGGTAACGCCAACCATCATAATTACTGCTGACAAATAAAATTTGCACCTAAG
CGCATCGTCTGGGAATTCTC

Downstream 100 bases:

>100_bases
AAAATTACAAATCGTAACATAAATCATCATTTATCATCCATCTTTGATGATTACTGAAGTATGAGTTAATCTTTCAGCTC
CAGAGCATCTTAAAATTGTG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
METKTAKILDGKTLAEKIQKELTAQIIDAQAKIGRPPGLAVLMVGDNPASAAYVRNKEKSCAKVGIASFGKHFPQETTQT
ELEDVIAALNQDEQVDGILVQLPLPEHLDAVKLLHQIEPDKDADGLHPVNLGRLVRGEKGLRSCTPAGVMRLLAEYEISL
RGKQAVVVGRSILVGKPMALMLLEADATVTIAHSRSQDLKSITQNADILIAAAGLPGLITADMVKPGAVVVDVGINRVSD
AHGKSRLVGDINFASIAGVAEYITPVPGGIGPMTVALLLQNTVTSYLQTAKESGALDVK

Sequences:

>Translated_299_residues
METKTAKILDGKTLAEKIQKELTAQIIDAQAKIGRPPGLAVLMVGDNPASAAYVRNKEKSCAKVGIASFGKHFPQETTQT
ELEDVIAALNQDEQVDGILVQLPLPEHLDAVKLLHQIEPDKDADGLHPVNLGRLVRGEKGLRSCTPAGVMRLLAEYEISL
RGKQAVVVGRSILVGKPMALMLLEADATVTIAHSRSQDLKSITQNADILIAAAGLPGLITADMVKPGAVVVDVGINRVSD
AHGKSRLVGDINFASIAGVAEYITPVPGGIGPMTVALLLQNTVTSYLQTAKESGALDVK
>Mature_299_residues
METKTAKILDGKTLAEKIQKELTAQIIDAQAKIGRPPGLAVLMVGDNPASAAYVRNKEKSCAKVGIASFGKHFPQETTQT
ELEDVIAALNQDEQVDGILVQLPLPEHLDAVKLLHQIEPDKDADGLHPVNLGRLVRGEKGLRSCTPAGVMRLLAEYEISL
RGKQAVVVGRSILVGKPMALMLLEADATVTIAHSRSQDLKSITQNADILIAAAGLPGLITADMVKPGAVVVDVGINRVSD
AHGKSRLVGDINFASIAGVAEYITPVPGGIGPMTVALLLQNTVTSYLQTAKESGALDVK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222418558, Length=289, Percent_Identity=47.4048442906574, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI94721354, Length=289, Percent_Identity=45.6747404844291, Blast_Score=237, Evalue=9e-63,
Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=40.2777777777778, Blast_Score=211, Evalue=9e-55,
Organism=Homo sapiens, GI36796743, Length=205, Percent_Identity=25.8536585365854, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=48.943661971831, Blast_Score=275, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17568735, Length=288, Percent_Identity=38.8888888888889, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319558, Length=299, Percent_Identity=42.4749163879599, Blast_Score=230, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=38.3333333333333, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6322933, Length=312, Percent_Identity=25.9615384615385, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=45.9183673469388, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI17136818, Length=291, Percent_Identity=46.3917525773196, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI62472483, Length=286, Percent_Identity=41.958041958042, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI45551871, Length=286, Percent_Identity=41.958041958042, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24645718, Length=286, Percent_Identity=41.958041958042, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17137370, Length=286, Percent_Identity=41.958041958042, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31551; Mature: 31551

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKTAKILDGKTLAEKIQKELTAQIIDAQAKIGRPPGLAVLMVGDNPASAAYVRNKEKS
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHCCHHH
CAKVGIASFGKHFPQETTQTELEDVIAALNQDEQVDGILVQLPLPEHLDAVKLLHQIEPD
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCC
KDADGLHPVNLGRLVRGEKGLRSCTPAGVMRLLAEYEISLRGKQAVVVGRSILVGKPMAL
CCCCCCCCCCHHHHHHCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEECCHHECCCCEEE
MLLEADATVTIAHSRSQDLKSITQNADILIAAAGLPGLITADMVKPGAVVVDVGINRVSD
EEEECCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCCEEHHHCCCCCEEEECCCCHHHC
AHGKSRLVGDINFASIAGVAEYITPVPGGIGPMTVALLLQNTVTSYLQTAKESGALDVK
CCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
METKTAKILDGKTLAEKIQKELTAQIIDAQAKIGRPPGLAVLMVGDNPASAAYVRNKEKS
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHCCHHH
CAKVGIASFGKHFPQETTQTELEDVIAALNQDEQVDGILVQLPLPEHLDAVKLLHQIEPD
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCC
KDADGLHPVNLGRLVRGEKGLRSCTPAGVMRLLAEYEISLRGKQAVVVGRSILVGKPMAL
CCCCCCCCCCHHHHHHCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEECCHHECCCCEEE
MLLEADATVTIAHSRSQDLKSITQNADILIAAAGLPGLITADMVKPGAVVVDVGINRVSD
EEEECCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCCEEHHHCCCCCEEEECCCCHHHC
AHGKSRLVGDINFASIAGVAEYITPVPGGIGPMTVALLLQNTVTSYLQTAKESGALDVK
CCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA