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The map label for this gene is hsdS

Identifier: 16767768

GI number: 16767768

Start: 4777606

End: 4779015

Strand: Reverse

Name: hsdS

Synonym: STM4524

Alternate gene names: 16767768

Gene position: 4779015-4777606 (Counterclockwise)

Preceding gene: 16767769

Following gene: 16767767

Centisome position: 98.39

GC content: 40.92

Gene sequence:

>1410_bases
ATGAGTGGGGGGAAATTGCCGGAGGGGTGGGCTACAAGCACAATCAATGAAATGTGCAACCTCAATCCCAAACTTAAACT
TGATGATGATTTAGATGTTGGATTTATGCCGATGGCCGGTGTTCCAACAACCTATTTAGGTAAATGTAACTTTGAAACCA
AAAAGTGGAGTGAAGTAAAAAAAGGATTTACTCAATTTCAGAACGATGATGTTATTTTTGCCAAAATCACGCCATGCTTC
GAAAATGGAAAAGCTGTTGTAATCAAAGAATTCCCTAACGGCTATGGTGCCGGTAGCACTGAATATTATGTTCTTCGGTC
TATTAATGGGTTAATTAATCCCCATTGGTTGTTTGCTTTAGTTAAAACTAAAGATTTTTTAACTAATGGAGCACTTAATA
TGTCCGGTTCAGTCGGACATAAACGTGTTACTAAAGAATTTCTTGAGAACTATGGTGTTCCTGTCCCACCTCTTGCCGAA
CAAAAAGTCATCGCCGAAAAACTCGATACGCTGCTGGCGCAGGTAGACAGCACCAAAGCACGTCTTGAGCAAATCCCACA
AATCCTGAAACGTTTTCGCCAATCAGTGATAGTTGCAGCAGTAAACGGGCAACTGACAAAAGAACTTCATAAAAAAAATA
AATTCAAGTTAACAGAATTGAATATTTCTATTCCATCTTTATGGAAAATCAGTGAGATTGGTCAATTTGCTGATGTCAAA
GGTGGCAAGAGATTACCTAAAGGTGAATCATTAATAGCTGAGAATACAGGGTTTCCATATATTAGAGCAGGGCAGCTAAA
AAATGGAACTGTTCTTCCTGAAGGACAACTATACTTAGAAGAATATATACAAAAAAGTATTTCTAGATATACAGTCTCAT
CTGGAGATCTTTATATAACTATTGTTGGTGCATGTATTGGTGATGCTGGTATAATTCCAGATGTTTATAATAATGCAAAT
TTAACTGAAAATGCTGCCAAGATATGTAATTTAAACGAAAATATTTTCAATAGATTTCTTTCTTTATGGCTGAGAAGTAG
TTATCTTCAGGATATTATCAATTCAGAAATAAAATCAGGAGCTCAAGGGAAGTTGGCTTTGGCAAGGATAAAATCACTCC
CATTGATACTACCACCACTCCAAGAACAACACGAAATCGTCCGCCGCGTCGAACAACTCTTCGCCTACGCCGACACCATT
GAAAAGCAGGTCAACAACGCCCTGACCCGCGTCAACAGCCTCACCCAGTCGATCCTGGCGAAGGCCTTCCGCGGCGAGCT
GACCGCCCAGTGGCGTGCGGAAAACCCTGAACTTATCAGCGGTGAAAACAGCGCCGCCGCCCTGCTGGAAAAAATTAAGG
CCGAACGCGCCGCCAGCGGCGGTAAAAAAACCTCGCGTAAAAAAGCCTGA

Upstream 100 bases:

>100_bases
CGCTGGGCGAACTGGATGCGCTGATGCGCGAGCTGGGCGCGGGCGATGAAGCGGATGCTCAGCGTCAGTTGCTGAATGAA
GCGTTTGGCGAGGTGAAGGC

Downstream 100 bases:

>100_bases
CCTTTATTTTCAAGCGCACCTTCCCGGTGCGCTTTTTATTATTTCCCCGCAATCATAACCATCAAAAATATATCCAGTTT
ATTCCTGAACATTCCCATTA

Product: type I restriction enzyme specificity protein

Products: NA

Alternate protein names: S.StySJI; Type I restriction enzyme StySJI specificity protein; S protein

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSGGKLPEGWATSTINEMCNLNPKLKLDDDLDVGFMPMAGVPTTYLGKCNFETKKWSEVKKGFTQFQNDDVIFAKITPCF
ENGKAVVIKEFPNGYGAGSTEYYVLRSINGLINPHWLFALVKTKDFLTNGALNMSGSVGHKRVTKEFLENYGVPVPPLAE
QKVIAEKLDTLLAQVDSTKARLEQIPQILKRFRQSVIVAAVNGQLTKELHKKNKFKLTELNISIPSLWKISEIGQFADVK
GGKRLPKGESLIAENTGFPYIRAGQLKNGTVLPEGQLYLEEYIQKSISRYTVSSGDLYITIVGACIGDAGIIPDVYNNAN
LTENAAKICNLNENIFNRFLSLWLRSSYLQDIINSEIKSGAQGKLALARIKSLPLILPPLQEQHEIVRRVEQLFAYADTI
EKQVNNALTRVNSLTQSILAKAFRGELTAQWRAENPELISGENSAAALLEKIKAERAASGGKKTSRKKA

Sequences:

>Translated_469_residues
MSGGKLPEGWATSTINEMCNLNPKLKLDDDLDVGFMPMAGVPTTYLGKCNFETKKWSEVKKGFTQFQNDDVIFAKITPCF
ENGKAVVIKEFPNGYGAGSTEYYVLRSINGLINPHWLFALVKTKDFLTNGALNMSGSVGHKRVTKEFLENYGVPVPPLAE
QKVIAEKLDTLLAQVDSTKARLEQIPQILKRFRQSVIVAAVNGQLTKELHKKNKFKLTELNISIPSLWKISEIGQFADVK
GGKRLPKGESLIAENTGFPYIRAGQLKNGTVLPEGQLYLEEYIQKSISRYTVSSGDLYITIVGACIGDAGIIPDVYNNAN
LTENAAKICNLNENIFNRFLSLWLRSSYLQDIINSEIKSGAQGKLALARIKSLPLILPPLQEQHEIVRRVEQLFAYADTI
EKQVNNALTRVNSLTQSILAKAFRGELTAQWRAENPELISGENSAAALLEKIKAERAASGGKKTSRKKA
>Mature_468_residues
SGGKLPEGWATSTINEMCNLNPKLKLDDDLDVGFMPMAGVPTTYLGKCNFETKKWSEVKKGFTQFQNDDVIFAKITPCFE
NGKAVVIKEFPNGYGAGSTEYYVLRSINGLINPHWLFALVKTKDFLTNGALNMSGSVGHKRVTKEFLENYGVPVPPLAEQ
KVIAEKLDTLLAQVDSTKARLEQIPQILKRFRQSVIVAAVNGQLTKELHKKNKFKLTELNISIPSLWKISEIGQFADVKG
GKRLPKGESLIAENTGFPYIRAGQLKNGTVLPEGQLYLEEYIQKSISRYTVSSGDLYITIVGACIGDAGIIPDVYNNANL
TENAAKICNLNENIFNRFLSLWLRSSYLQDIINSEIKSGAQGKLALARIKSLPLILPPLQEQHEIVRRVEQLFAYADTIE
KQVNNALTRVNSLTQSILAKAFRGELTAQWRAENPELISGENSAAALLEKIKAERAASGGKKTSRKKA

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family

Homologues:

Organism=Escherichia coli, GI1790807, Length=506, Percent_Identity=40.3162055335968, Blast_Score=274, Evalue=8e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): T1S_SALTY (P06187)

Other databases:

- EMBL:   Y00524
- EMBL:   AE006468
- EMBL:   K03551
- RefSeq:   NP_463383.1
- ProteinModelPortal:   P06187
- REBASE:   3686
- REBASE:   3700
- REBASE:   5595
- PRIDE:   P06187
- GeneID:   1256050
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM4524
- NMPDR:   fig|99287.1.peg.4352
- HOGENOM:   HBG754227
- OMA:   LFAYADQ
- ProtClustDB:   CLSK2302031
- BioCyc:   STYP99287:STM4524-MONOMER
- InterPro:   IPR000055

Pfam domain/function: PF01420 Methylase_S

EC number: NA

Molecular weight: Translated: 51910; Mature: 51779

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGGKLPEGWATSTINEMCNLNPKLKLDDDLDVGFMPMAGVPTTYLGKCNFETKKWSEVK
CCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHH
KGFTQFQNDDVIFAKITPCFENGKAVVIKEFPNGYGAGSTEYYVLRSINGLINPHWLFAL
HHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCEEEEEHHCCCCCHHHHEEH
VKTKDFLTNGALNMSGSVGHKRVTKEFLENYGVPVPPLAEQKVIAEKLDTLLAQVDSTKA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RLEQIPQILKRFRQSVIVAAVNGQLTKELHKKNKFKLTELNISIPSLWKISEIGQFADVK
HHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCEEEEEEEECCCCCCCHHHHCHHHHCC
GGKRLPKGESLIAENTGFPYIRAGQLKNGTVLPEGQLYLEEYIQKSISRYTVSSGDLYIT
CCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCEEEE
IVGACIGDAGIIPDVYNNANLTENAAKICNLNENIFNRFLSLWLRSSYLQDIINSEIKSG
EEEHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AQGKLALARIKSLPLILPPLQEQHEIVRRVEQLFAYADTIEKQVNNALTRVNSLTQSILA
CCCCHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAFRGELTAQWRAENPELISGENSAAALLEKIKAERAASGGKKTSRKKA
HHHHCCCEEEECCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SGGKLPEGWATSTINEMCNLNPKLKLDDDLDVGFMPMAGVPTTYLGKCNFETKKWSEVK
CCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHH
KGFTQFQNDDVIFAKITPCFENGKAVVIKEFPNGYGAGSTEYYVLRSINGLINPHWLFAL
HHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCEEEEEHHCCCCCHHHHEEH
VKTKDFLTNGALNMSGSVGHKRVTKEFLENYGVPVPPLAEQKVIAEKLDTLLAQVDSTKA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RLEQIPQILKRFRQSVIVAAVNGQLTKELHKKNKFKLTELNISIPSLWKISEIGQFADVK
HHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCEEEEEEEECCCCCCCHHHHCHHHHCC
GGKRLPKGESLIAENTGFPYIRAGQLKNGTVLPEGQLYLEEYIQKSISRYTVSSGDLYIT
CCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCEEEE
IVGACIGDAGIIPDVYNNANLTENAAKICNLNENIFNRFLSLWLRSSYLQDIINSEIKSG
EEEHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AQGKLALARIKSLPLILPPLQEQHEIVRRVEQLFAYADTIEKQVNNALTRVNSLTQSILA
CCCCHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAFRGELTAQWRAENPELISGENSAAALLEKIKAERAASGGKKTSRKKA
HHHHCCCEEEECCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2838725; 11677609; 3025838