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The map label for this gene is nrfD [H]

Identifier: 16767530

GI number: 16767530

Start: 4518883

End: 4519839

Strand: Direct

Name: nrfD [H]

Synonym: STM4280

Alternate gene names: 16767530

Gene position: 4518883-4519839 (Clockwise)

Preceding gene: 16767529

Following gene: 16767531

Centisome position: 93.03

GC content: 59.04

Gene sequence:

>957_bases
ATGACATCCGCTTCTGCATTTCATTTTGCATCACTGGTGTGGGACTGGCCCATCGCCATCTATCTGTTTCTGATTGGCAT
CTCCGCCGGACTGGTTACGTTGGCGATTCTGCTGCGCCGCTTCCATCCGGAGGCGGGCGGTTCGGACAGTACGCTGCTGC
GCACTACGCTGGTGTTAGGGCCGGGGGCGATTATTCTTGGTCTGCTGATTCTGGTCTTTCACCTGACGCGCCCGTGGACC
TTCTGGAAGCTGATGTTCCACTACAGCTTTACCTCGGTGATGTCGATGGGGGTGATGCTGTTCCAGTTGTACATGGTAGT
GCTGGTGCTGTGGCTGGCGAAAATCTTTGAAAAAGAGGTGATTGCCCTGCAACAGCGTTGGCTGCCGCGGTCAGGGATCG
TGCAGAAAGTGCTGACGCTGATCGCGCCGTTTCATCGCGCGCTGGAGACGTTAATGCTGGTACTGGCGGTTCTGCTCGGG
GCGTACACCGGCTTTCTGCTGTCGGCGCTGAAATCCTATCCGTTCCTGAATAACCCGATCCTGCCGGCGCTGTTTCTGTT
CTCCGGCATCTCCTCCGGCGCGGCGGTGGCGCTGATCGCTATGGCGTTACGCCATCGTAGCAACCCGCACAGCACCGAGG
CGCGCTTTGTACATCGCATTGAAACCCCAGTGGTATGGCTGGAGATCTTCCTGCTGGCGGCCTTTTTTATCGGGCTGGCG
CTGGGCGATGACGGCAAAATGCGCGCGCTGGCGGCGGCGCTGGGCGGCGGATTCTGGAGCTGGTGGTTCTGGCTTGGCGT
CGTAGGGCTGGGATTGATTATCCCGCTGCTGCTGAAGCCCTGGGCAAACCGTAGCGTCACGTTTCACGGCGTGCTGGCCG
TTTGCGGCGCCAGTTTGACCGGCGTGCTGCTGCTGCGCTTTTTCATTCTTTATGCGGGACAGCTAACGGTTGCATAA

Upstream 100 bases:

>100_bases
CCCGTTTGTTGCGGCAGAAAACCACCTATCGTTACAAGCTGGCGCTGGGCACAAAACCGAAGGTCTACCGCGTTCCCTTT
AACTATGGGGAGGTGAGCCA

Downstream 100 bases:

>100_bases
TCTGGACATCTTCCTTCCTGAAGCCGGATTTCTGGCGCTGTTGTTGAGTCTCGGGGTCAACGTGCTGACCCCGCTGGCGA
CGTGGTGCGGGATGGGGCAG

Product: formate-dependent nitrate reductase

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTSASAFHFASLVWDWPIAIYLFLIGISAGLVTLAILLRRFHPEAGGSDSTLLRTTLVLGPGAIILGLLILVFHLTRPWT
FWKLMFHYSFTSVMSMGVMLFQLYMVVLVLWLAKIFEKEVIALQQRWLPRSGIVQKVLTLIAPFHRALETLMLVLAVLLG
AYTGFLLSALKSYPFLNNPILPALFLFSGISSGAAVALIAMALRHRSNPHSTEARFVHRIETPVVWLEIFLLAAFFIGLA
LGDDGKMRALAAALGGGFWSWWFWLGVVGLGLIIPLLLKPWANRSVTFHGVLAVCGASLTGVLLLRFFILYAGQLTVA

Sequences:

>Translated_318_residues
MTSASAFHFASLVWDWPIAIYLFLIGISAGLVTLAILLRRFHPEAGGSDSTLLRTTLVLGPGAIILGLLILVFHLTRPWT
FWKLMFHYSFTSVMSMGVMLFQLYMVVLVLWLAKIFEKEVIALQQRWLPRSGIVQKVLTLIAPFHRALETLMLVLAVLLG
AYTGFLLSALKSYPFLNNPILPALFLFSGISSGAAVALIAMALRHRSNPHSTEARFVHRIETPVVWLEIFLLAAFFIGLA
LGDDGKMRALAAALGGGFWSWWFWLGVVGLGLIIPLLLKPWANRSVTFHGVLAVCGASLTGVLLLRFFILYAGQLTVA
>Mature_317_residues
TSASAFHFASLVWDWPIAIYLFLIGISAGLVTLAILLRRFHPEAGGSDSTLLRTTLVLGPGAIILGLLILVFHLTRPWTF
WKLMFHYSFTSVMSMGVMLFQLYMVVLVLWLAKIFEKEVIALQQRWLPRSGIVQKVLTLIAPFHRALETLMLVLAVLLGA
YTGFLLSALKSYPFLNNPILPALFLFSGISSGAAVALIAMALRHRSNPHSTEARFVHRIETPVVWLEIFLLAAFFIGLAL
GDDGKMRALAAALGGGFWSWWFWLGVVGLGLIIPLLLKPWANRSVTFHGVLAVCGASLTGVLLLRFFILYAGQLTVA

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=318, Percent_Identity=85.8490566037736, Blast_Score=547, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 35107; Mature: 34976

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSASAFHFASLVWDWPIAIYLFLIGISAGLVTLAILLRRFHPEAGGSDSTLLRTTLVLG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
PGAIILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQLYMVVLVLWLAKIFEKEV
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALQQRWLPRSGIVQKVLTLIAPFHRALETLMLVLAVLLGAYTGFLLSALKSYPFLNNPI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
LPALFLFSGISSGAAVALIAMALRHRSNPHSTEARFVHRIETPVVWLEIFLLAAFFIGLA
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
LGDDGKMRALAAALGGGFWSWWFWLGVVGLGLIIPLLLKPWANRSVTFHGVLAVCGASLT
CCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
GVLLLRFFILYAGQLTVA
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TSASAFHFASLVWDWPIAIYLFLIGISAGLVTLAILLRRFHPEAGGSDSTLLRTTLVLG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC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HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]