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The map label for this gene is nrfE [H]

Identifier: 16767531

GI number: 16767531

Start: 4519832

End: 4522054

Strand: Direct

Name: nrfE [H]

Synonym: STM4281

Alternate gene names: 16767531

Gene position: 4519832-4522054 (Clockwise)

Preceding gene: 16767530

Following gene: 16767532

Centisome position: 93.05

GC content: 58.84

Gene sequence:

>2223_bases
TTGCATAATCTGGACATCTTCCTTCCTGAAGCCGGATTTCTGGCGCTGTTGTTGAGTCTCGGGGTCAACGTGCTGACCCC
GCTGGCGACGTGGTGCGGGATGGGGCAGCACTGGCGCGGCGTCATGCGCCTGACGACCTGCGGCGCGTGGACGGCGTTCA
CGCTGCTGCTACTGGCGTTTGCGATTCTGGTTAGCTGTTTTCTGACCAGCGATTTTTCGGTCGTTTATGTTGCGCAGCAT
AGCCATAGTCAGTTGTCATGGGGACTGAAACTGGCGGCGGTATGGGGCGGTCATGAAGGTTCGCTCCTGCTGTGGGCGCT
GCTTCTTTCGGGGTGGACGGCGCTATTCGCCTGGCGCTCCCGGCATGAATCTGACGCGCTTTTTCCGCTGACCCTGAGCA
TCTTGTCCTTCATTATGGCCTCGCTGCTGCTATTTATTGTGCTGTGGTCCGATCCGTTCCTGCGTATTTTCCCTCCCGCC
ATGGAAGGGCGCGACCTTAATCCCATGCTGCAACATCTGGGGCTGATCTTACATCCGCCGCTGCTCTATCTGGGTTATGG
CGGCCTGATGACCGCGGCGAGCGTAGCGCTGGCTTCGCTACTGTGCGGCGGCTTTAACGCCACAACCGCCTGGGTTTGCT
GGCGCTGGGCGTTACCGGGTTGGTGCGCTCTGACGCTGGGGATCATTCTCGGATCGTGGTGGGCATATTGCGAACTGGGC
TGGGGCGGCTGGTGGTTCTGGGACCCGGTGGAAAATGCCTCGTTGTTGCCGTGGCTTGCCGCCAGCGCGTTGCTGCACAG
TCTGTATGTCAGCCGCCAGCGCGGGAGCTTCCGCCACTGGTCGTTGCTGCTGGCGATACTGACGCTGATCCTCTCGTTGC
TGGGAACGCTGATTGTGCGTTCCGGCATTCTGCTCTCCGTCCATGCTTTTGCGCTGGATAACGTGCGCGCGGTGCCGCTG
TTTATCCTTTTTGCCGCGTTAAGTCTGGCATCGCTTGCTCTGTACGGCTGGCGGGCACGGGAGCCGTATCCGGCGACGCG
TTTGGGCGGCGGAAAGTGCGAAACGCTCATTCTGACGACGCTGCTGCTCTTTAGCGCCGTCTTGTTGATCGTGCTGGTCG
GGACGCTCTACCCCATGATTTATGGTTTGATGGGCTGGGGAAGGCTTTCGGTGGGCGCGCCCTATTTTAATCGCGTCACG
CTGCCGTTTGGCCTGCTGATGCTGGTAGTTATCGTGCTGGCGACAATCAGAAGCCGCAAAGTCTCTGTGCGTTGTCAGCT
TCCGGCATTGCTGGCGCATACGGGCGTATTTATCTTCGCCGCCGGGATCATGGTTTCCAGCGGCAGTCGGCATGAAATCA
GCCTTAACCTCAGCCCCGGTCAGCAGGTGGTACTGGCGGGCTATACCTTTCGTTTCGAGCGTCTGGATCTGGAAGCCAAA
GGCAACTACACCAGTGAAAAAGCGCGGATCACGTTGTGGCGGAATGAAAAACGCATTGGCAGCCTGCAGCCTGAAAGGCG
CTTCTATGCCGCGCGTCGTCAACAGATGATGGAGCCGGGGATTCACTGGAACCTGCTACATGACTGGTACGCGGTGATGG
GCGAAAAAACCGGACCGGATCGTTACGCCATGCGCTTATACGTGCAGACCGGCGTGCGCTGGATCTGGGGTGGGGGATTG
TTGATGGTGTGTGGAGCGCTGCTGAGCGGATGGCGGGGGAGGAAGCATCCTGAGTTATTGCCTGATGGCGCTGCGCTTAT
CAGGCCTACGAGTGAAAAGCATGGTAGGCCGGATAAGGCGTTCACGCCGCCATCCGGCAAAACGATGTTACTTTTGGCTT
TACTGATTTTTCTGCCTTTTGCCACCCATGCCCAGGTGGTGGACACATGGACGTTTGCCAACCCGCAGCAGCAGGAGAAA
GCGCTGTCTATTGCCAGTCAGCTGCGCTGTCCGCAGTGTCAGAACCAGAACTTGCTGGAATCCAATGCGCCCGTGGCGGT
CAGTATGCGCCATCAGGTTTACAGCATGGTTGCCGAAGGTAAAAGCGAAGCGGAAATCACCGCCTGGATGACCGACCGCT
ATGGTGATTTCGTGCGCTACAACCCGCCGCTGAATGAGCAAACGCTGCTGTTGTGGGCGCTGCCGTACCTGCTGTTATTG
CTGGTGGGCGTGGTGGCCTGGCGGGTGAGAAAGCGTCAGCACGCAGGAGAGGGGGAACAATGA

Upstream 100 bases:

>100_bases
CGTAGCGTCACGTTTCACGGCGTGCTGGCCGTTTGCGGCGCCAGTTTGACCGGCGTGCTGCTGCTGCGCTTTTTCATTCT
TTATGCGGGACAGCTAACGG

Downstream 100 bases:

>100_bases
GCCAGTCTGAACACTCCACTGCGCCGTTACGTCCCATGCCTGTAAAGCGTCTGGCGGCTGCGGCGGTGTTGATGGTGGCT
GCCTGTGTCGGCGGCTATCT

Product: formate-dependent nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 740; Mature: 740

Protein sequence:

>740_residues
MHNLDIFLPEAGFLALLLSLGVNVLTPLATWCGMGQHWRGVMRLTTCGAWTAFTLLLLAFAILVSCFLTSDFSVVYVAQH
SHSQLSWGLKLAAVWGGHEGSLLLWALLLSGWTALFAWRSRHESDALFPLTLSILSFIMASLLLFIVLWSDPFLRIFPPA
MEGRDLNPMLQHLGLILHPPLLYLGYGGLMTAASVALASLLCGGFNATTAWVCWRWALPGWCALTLGIILGSWWAYCELG
WGGWWFWDPVENASLLPWLAASALLHSLYVSRQRGSFRHWSLLLAILTLILSLLGTLIVRSGILLSVHAFALDNVRAVPL
FILFAALSLASLALYGWRAREPYPATRLGGGKCETLILTTLLLFSAVLLIVLVGTLYPMIYGLMGWGRLSVGAPYFNRVT
LPFGLLMLVVIVLATIRSRKVSVRCQLPALLAHTGVFIFAAGIMVSSGSRHEISLNLSPGQQVVLAGYTFRFERLDLEAK
GNYTSEKARITLWRNEKRIGSLQPERRFYAARRQQMMEPGIHWNLLHDWYAVMGEKTGPDRYAMRLYVQTGVRWIWGGGL
LMVCGALLSGWRGRKHPELLPDGAALIRPTSEKHGRPDKAFTPPSGKTMLLLALLIFLPFATHAQVVDTWTFANPQQQEK
ALSIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEAEITAWMTDRYGDFVRYNPPLNEQTLLLWALPYLLLL
LVGVVAWRVRKRQHAGEGEQ

Sequences:

>Translated_740_residues
MHNLDIFLPEAGFLALLLSLGVNVLTPLATWCGMGQHWRGVMRLTTCGAWTAFTLLLLAFAILVSCFLTSDFSVVYVAQH
SHSQLSWGLKLAAVWGGHEGSLLLWALLLSGWTALFAWRSRHESDALFPLTLSILSFIMASLLLFIVLWSDPFLRIFPPA
MEGRDLNPMLQHLGLILHPPLLYLGYGGLMTAASVALASLLCGGFNATTAWVCWRWALPGWCALTLGIILGSWWAYCELG
WGGWWFWDPVENASLLPWLAASALLHSLYVSRQRGSFRHWSLLLAILTLILSLLGTLIVRSGILLSVHAFALDNVRAVPL
FILFAALSLASLALYGWRAREPYPATRLGGGKCETLILTTLLLFSAVLLIVLVGTLYPMIYGLMGWGRLSVGAPYFNRVT
LPFGLLMLVVIVLATIRSRKVSVRCQLPALLAHTGVFIFAAGIMVSSGSRHEISLNLSPGQQVVLAGYTFRFERLDLEAK
GNYTSEKARITLWRNEKRIGSLQPERRFYAARRQQMMEPGIHWNLLHDWYAVMGEKTGPDRYAMRLYVQTGVRWIWGGGL
LMVCGALLSGWRGRKHPELLPDGAALIRPTSEKHGRPDKAFTPPSGKTMLLLALLIFLPFATHAQVVDTWTFANPQQQEK
ALSIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEAEITAWMTDRYGDFVRYNPPLNEQTLLLWALPYLLLL
LVGVVAWRVRKRQHAGEGEQ
>Mature_740_residues
MHNLDIFLPEAGFLALLLSLGVNVLTPLATWCGMGQHWRGVMRLTTCGAWTAFTLLLLAFAILVSCFLTSDFSVVYVAQH
SHSQLSWGLKLAAVWGGHEGSLLLWALLLSGWTALFAWRSRHESDALFPLTLSILSFIMASLLLFIVLWSDPFLRIFPPA
MEGRDLNPMLQHLGLILHPPLLYLGYGGLMTAASVALASLLCGGFNATTAWVCWRWALPGWCALTLGIILGSWWAYCELG
WGGWWFWDPVENASLLPWLAASALLHSLYVSRQRGSFRHWSLLLAILTLILSLLGTLIVRSGILLSVHAFALDNVRAVPL
FILFAALSLASLALYGWRAREPYPATRLGGGKCETLILTTLLLFSAVLLIVLVGTLYPMIYGLMGWGRLSVGAPYFNRVT
LPFGLLMLVVIVLATIRSRKVSVRCQLPALLAHTGVFIFAAGIMVSSGSRHEISLNLSPGQQVVLAGYTFRFERLDLEAK
GNYTSEKARITLWRNEKRIGSLQPERRFYAARRQQMMEPGIHWNLLHDWYAVMGEKTGPDRYAMRLYVQTGVRWIWGGGL
LMVCGALLSGWRGRKHPELLPDGAALIRPTSEKHGRPDKAFTPPSGKTMLLLALLIFLPFATHAQVVDTWTFANPQQQEK
ALSIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEAEITAWMTDRYGDFVRYNPPLNEQTLLLWALPYLLLL
LVGVVAWRVRKRQHAGEGEQ

Specific function: May be required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG1138

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family [H]

Homologues:

Organism=Escherichia coli, GI1790511, Length=555, Percent_Identity=76.9369369369369, Blast_Score=784, Evalue=0.0,
Organism=Escherichia coli, GI1788524, Length=642, Percent_Identity=36.6043613707165, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1790512, Length=92, Percent_Identity=84.7826086956522, Blast_Score=163, Evalue=4e-41,
Organism=Escherichia coli, GI1788522, Length=118, Percent_Identity=36.4406779661017, Blast_Score=99, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002541
- InterPro:   IPR003567
- InterPro:   IPR003568
- InterPro:   IPR003570 [H]

Pfam domain/function: PF01578 Cytochrom_C_asm [H]

EC number: 2.1.1.-

Molecular weight: Translated: 82452; Mature: 82452

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNLDIFLPEAGFLALLLSLGVNVLTPLATWCGMGQHWRGVMRLTTCGAWTAFTLLLLAF
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
AILVSCFLTSDFSVVYVAQHSHSQLSWGLKLAAVWGGHEGSLLLWALLLSGWTALFAWRS
HHHHHHHHCCCCCEEEEEECCCCCHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RHESDALFPLTLSILSFIMASLLLFIVLWSDPFLRIFPPAMEGRDLNPMLQHLGLILHPP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHCHHHHCCH
LLYLGYGGLMTAASVALASLLCGGFNATTAWVCWRWALPGWCALTLGIILGSWWAYCELG
HHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHEEHHCCCHHHHHHHHHHHHCHHHHEEEC
WGGWWFWDPVENASLLPWLAASALLHSLYVSRQRGSFRHWSLLLAILTLILSLLGTLIVR
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SGILLSVHAFALDNVRAVPLFILFAALSLASLALYGWRAREPYPATRLGGGKCETLILTT
CCHHEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LLLFSAVLLIVLVGTLYPMIYGLMGWGRLSVGAPYFNRVTLPFGLLMLVVIVLATIRSRK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
VSVRCQLPALLAHTGVFIFAAGIMVSSGSRHEISLNLSPGQQVVLAGYTFRFERLDLEAK
EEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEEEEEEEEEEEEC
GNYTSEKARITLWRNEKRIGSLQPERRFYAARRQQMMEPGIHWNLLHDWYAVMGEKTGPD
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
RYAMRLYVQTGVRWIWGGGLLMVCGALLSGWRGRKHPELLPDGAALIRPTSEKHGRPDKA
HHHHEEEHHHCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCHHCCCCCCC
FTPPSGKTMLLLALLIFLPFATHAQVVDTWTFANPQQQEKALSIASQLRCPQCQNQNLLE
CCCCCCCHHHHHHHHHHHCCCCCHHHHHEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
SNAPVAVSMRHQVYSMVAEGKSEAEITAWMTDRYGDFVRYNPPLNEQTLLLWALPYLLLL
CCCCEEEEHHHHHHHHHHCCCCCCEEEEEEHHCCCCCEEECCCCCCCHHHHHHHHHHHHH
LVGVVAWRVRKRQHAGEGEQ
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHNLDIFLPEAGFLALLLSLGVNVLTPLATWCGMGQHWRGVMRLTTCGAWTAFTLLLLAF
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
AILVSCFLTSDFSVVYVAQHSHSQLSWGLKLAAVWGGHEGSLLLWALLLSGWTALFAWRS
HHHHHHHHCCCCCEEEEEECCCCCHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RHESDALFPLTLSILSFIMASLLLFIVLWSDPFLRIFPPAMEGRDLNPMLQHLGLILHPP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHCHHHHCCH
LLYLGYGGLMTAASVALASLLCGGFNATTAWVCWRWALPGWCALTLGIILGSWWAYCELG
HHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHEEHHCCCHHHHHHHHHHHHCHHHHEEEC
WGGWWFWDPVENASLLPWLAASALLHSLYVSRQRGSFRHWSLLLAILTLILSLLGTLIVR
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SGILLSVHAFALDNVRAVPLFILFAALSLASLALYGWRAREPYPATRLGGGKCETLILTT
CCHHEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LLLFSAVLLIVLVGTLYPMIYGLMGWGRLSVGAPYFNRVTLPFGLLMLVVIVLATIRSRK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
VSVRCQLPALLAHTGVFIFAAGIMVSSGSRHEISLNLSPGQQVVLAGYTFRFERLDLEAK
EEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEEEEEEEEEEEEC
GNYTSEKARITLWRNEKRIGSLQPERRFYAARRQQMMEPGIHWNLLHDWYAVMGEKTGPD
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
RYAMRLYVQTGVRWIWGGGLLMVCGALLSGWRGRKHPELLPDGAALIRPTSEKHGRPDKA
HHHHEEEHHHCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCHHCCCCCCC
FTPPSGKTMLLLALLIFLPFATHAQVVDTWTFANPQQQEKALSIASQLRCPQCQNQNLLE
CCCCCCCHHHHHHHHHHHCCCCCHHHHHEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
SNAPVAVSMRHQVYSMVAEGKSEAEITAWMTDRYGDFVRYNPPLNEQTLLLWALPYLLLL
CCCCEEEEHHHHHHHHHHCCCCCCEEEEEEHHCCCCCEEECCCCCCCHHHHHHHHHHHHH
LVGVVAWRVRKRQHAGEGEQ
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]