Click here to switch to the map view.

The map label for this gene is yjbN [H]

Identifier: 16767493

GI number: 16767493

Start: 4463960

End: 4464958

Strand: Direct

Name: yjbN [H]

Synonym: STM4243

Alternate gene names: 16767493

Gene position: 4463960-4464958 (Clockwise)

Preceding gene: 16767492

Following gene: 16767494

Centisome position: 91.9

GC content: 55.56

Gene sequence:

>999_bases
ATGCAGCCTGAAACCCAGTCCTCCGCTTTACCTGCTTATCGCTTCTCCATCGCGCCTATGCTCGACTGGACGGACAGACA
TTGCCGCTATTTCCTGCGTTTGCTGTCTCGCCAGACGCAGCTCTACACCGAAATGGTGACCACGGGCGCGATTATTCACG
GTAAGGGTGACTATCTGGCTTATAGCGAAGAAGAGCATCCGGTCGCTCTACAGCTTGGTGGAAGCGATCCGGCTCAGCTT
GCGCATTGTGCAAAGCTGGCGGAAGCGCGTGGCTACGATGAAATTAACCTCAACGTGGGGTGCCCTTCCGATCGCGTGCA
AAACGGTATGTTTGGCGCCTGTTTGATGGGCAATGCGCAACTGGTCGCCGATTGTGTTAAAGCCATGCGTGATGTCGTCT
CGATTCCGGTGACGGTAAAAACCCGCATTGGTATTGACGATCAGGACAGCTATGCGTTTCTGTGTGATTTCATCGATACG
GTTTCCGGTCAGGGCGAATGCGAGATGTTTATTATCCATGCGCGCAAAGCCTGGCTTTCTGGCTTAAGCCCGAAAGAAAA
TCGTGAGATCCCGCCGCTGGATTACCCGCGCGTCTATCAGCTAAAGCGGGATTTTCCGCACCTGACCATGTCCATTAACG
GCGGCATCAAATCGCTGGAAGAGGCGAAGGAGCACCTGCGCCATATGGATGGCGTCATGGTTGGCCGCGAAGCTTATCAG
AATCCGGGTATACTGGCCGCGGTGGATCGGGAGATTTTTGGCGCCGATACCACCGATGCCGACCCGGTTGCGGTGGTTCG
CGCGATGTATCCCTATATTGAGCGTGAATTGAGCCAGGGAGCGTATCTGGGGCATATCACTCGCCATATGCTGGGGTTGT
TCCAGGGCATCCCCGGCGCGCGGCAGTGGCGTCGCTATCTGAGCGAAAACGCCCATAAAGCTGGCGCGGATGTTGCTGTA
CTGGAGCAGGCGCTAAAACTGGTGGCAGACAAGCGTTAA

Upstream 100 bases:

>100_bases
AATGACATTCTGAGGTGCTTTCACCGGAGGCGCTACGCCTCCGGTTTAGCGTTATGCTATAGTAACGCCCCTTTTTTAAC
CGGATGCTTATTTATTCGCC

Downstream 100 bases:

>100_bases
AAGTTCGCCAAAAATTAGTCAATCTCACCACGCCCTGTGCAGTCTTGCAGGGCGTTTTTCTTATATATCAATAATATAAA
TATTGGCATGATTTTTGTAA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MQPETQSSALPAYRFSIAPMLDWTDRHCRYFLRLLSRQTQLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAQL
AHCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYAFLCDFIDT
VSGQGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLRHMDGVMVGREAYQ
NPGILAAVDREIFGADTTDADPVAVVRAMYPYIERELSQGAYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADVAV
LEQALKLVADKR

Sequences:

>Translated_332_residues
MQPETQSSALPAYRFSIAPMLDWTDRHCRYFLRLLSRQTQLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAQL
AHCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYAFLCDFIDT
VSGQGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLRHMDGVMVGREAYQ
NPGILAAVDREIFGADTTDADPVAVVRAMYPYIERELSQGAYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADVAV
LEQALKLVADKR
>Mature_332_residues
MQPETQSSALPAYRFSIAPMLDWTDRHCRYFLRLLSRQTQLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAQL
AHCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYAFLCDFIDT
VSGQGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLRHMDGVMVGREAYQ
NPGILAAVDREIFGADTTDADPVAVVRAMYPYIERELSQGAYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADVAV
LEQALKLVADKR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693211, Length=319, Percent_Identity=94.6708463949843, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1788462, Length=251, Percent_Identity=25.4980079681275, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=245, Percent_Identity=25.7142857142857, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24580595, Length=314, Percent_Identity=22.9299363057325, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19920448, Length=314, Percent_Identity=22.9299363057325, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36987; Mature: 36987

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPETQSSALPAYRFSIAPMLDWTDRHCRYFLRLLSRQTQLYTEMVTTGAIIHGKGDYLA
CCCCCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEE
YSEEEHPVALQLGGSDPAQLAHCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQ
ECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEECCCHH
LVADCVKAMRDVVSIPVTVKTRIGIDDQDSYAFLCDFIDTVSGQGECEMFIIHARKAWLS
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHC
GLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLRHMDGVMVGREAYQ
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHC
NPGILAAVDREIFGADTTDADPVAVVRAMYPYIERELSQGAYLGHITRHMLGLFQGIPGA
CCCEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
RQWRRYLSENAHKAGADVAVLEQALKLVADKR
HHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQPETQSSALPAYRFSIAPMLDWTDRHCRYFLRLLSRQTQLYTEMVTTGAIIHGKGDYLA
CCCCCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEE
YSEEEHPVALQLGGSDPAQLAHCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQ
ECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEECCCHH
LVADCVKAMRDVVSIPVTVKTRIGIDDQDSYAFLCDFIDTVSGQGECEMFIIHARKAWLS
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHC
GLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLRHMDGVMVGREAYQ
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHC
NPGILAAVDREIFGADTTDADPVAVVRAMYPYIERELSQGAYLGHITRHMLGLFQGIPGA
CCCEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
RQWRRYLSENAHKAGADVAVLEQALKLVADKR
HHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]