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The map label for this gene is pflC [H]

Identifier: 16767380

GI number: 16767380

Start: 4331077

End: 4331955

Strand: Direct

Name: pflC [H]

Synonym: STM4115

Alternate gene names: 16767380

Gene position: 4331077-4331955 (Clockwise)

Preceding gene: 16767379

Following gene: 16767381

Centisome position: 89.16

GC content: 54.95

Gene sequence:

>879_bases
ATGACATTATTCGCCGCACCGCGCATCAGTTGTGAGGTTGTGGGGGCGCGCGATGAGGAGACCGCGCGTATCTTCAATAT
CCAGCGCTATTCGCTGAATGACGGGCAGGGGATTCGTACGGTGGTCTTTTTTAAGGGCTGTCCGCATACCTGTCCGTGGT
GCGCTAATCCGGAATCTATCTCGCCGCGCATTGAAACGGTGCGCCGGGAAAATAAATGCCTGCGTTGCACGCCGTGTCTG
CGGGATGCCGACGAATGTCCTTCCGGCGCGTTTGAGCGTATTGGGCGTGATATTACGCTGGATGAACTGGAACGCGAAGT
GCTGAAAGACGACATCTTCTTTCGTACTTCCGGCGGCGGGGTGACGCTTTCTGGCGGCGAAGTGCTGATGCAGGCGCCGT
TCGCCACGCGCTTCTTACAGCGATTGCGGCGTTGGGGCGTTCCTTGTGCTATTGAAACGGCTGGCGATACGTCGGCCAGC
CGTCTACTGCCGTTGGCAAGGGCGTGTGATGAGGTATTGTTTGATCTGAAAATTATGGATGCGGAGCGGGCGCGCGAGGT
GATCAACATGAATCTGCCGCGCGTACTGGAGAATTTACGGTTACTGGTTAGCGAGGGAATCACGGTGATCCCTCGTTTGC
CGTTGATTCCTGGCTTTACGCTTAACGCAGAGAATCTGCAGTGCGCGTTAACATTGTTGAGATCATTAGGAATAAAGCAG
GTTCACCTGCTACCGTTTCATCAGTACGGCGAGCCGAAATACCGGCTGCTTGGAAAATCATGGATGATGAAAGATATTCC
CGCGCCGTCGGTGCAAGAGATCGCTCTGTTTCGGGAGATGGCGGAGCAAGCAGGATTTCAGGTCACCACAGGAGGTTAA

Upstream 100 bases:

>100_bases
TTGCGCGAAGCGCAGCTACGGCCTCAGGATTTCGCTGGGCTGGTGGTGCGCGTGGCGGGCTACAGCGCCTTCTTTGTCGA
GCTGTCGAAGGAGATTCAGG

Downstream 100 bases:

>100_bases
TATGGCAGTACCTGTACGGCATCTGGTGGCAGTGACCGCCTGCGTGAGCGGTGTAGCGCATACCTATATGGCTGCTGAAC
GGCTGGAAAAACTTTGCCAG

Product: pyruvate formate lyase II activase

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 2; PFL-activating enzyme 2 [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTLFAAPRISCEVVGARDEETARIFNIQRYSLNDGQGIRTVVFFKGCPHTCPWCANPESISPRIETVRRENKCLRCTPCL
RDADECPSGAFERIGRDITLDELEREVLKDDIFFRTSGGGVTLSGGEVLMQAPFATRFLQRLRRWGVPCAIETAGDTSAS
RLLPLARACDEVLFDLKIMDAERAREVINMNLPRVLENLRLLVSEGITVIPRLPLIPGFTLNAENLQCALTLLRSLGIKQ
VHLLPFHQYGEPKYRLLGKSWMMKDIPAPSVQEIALFREMAEQAGFQVTTGG

Sequences:

>Translated_292_residues
MTLFAAPRISCEVVGARDEETARIFNIQRYSLNDGQGIRTVVFFKGCPHTCPWCANPESISPRIETVRRENKCLRCTPCL
RDADECPSGAFERIGRDITLDELEREVLKDDIFFRTSGGGVTLSGGEVLMQAPFATRFLQRLRRWGVPCAIETAGDTSAS
RLLPLARACDEVLFDLKIMDAERAREVINMNLPRVLENLRLLVSEGITVIPRLPLIPGFTLNAENLQCALTLLRSLGIKQ
VHLLPFHQYGEPKYRLLGKSWMMKDIPAPSVQEIALFREMAEQAGFQVTTGG
>Mature_291_residues
TLFAAPRISCEVVGARDEETARIFNIQRYSLNDGQGIRTVVFFKGCPHTCPWCANPESISPRIETVRRENKCLRCTPCLR
DADECPSGAFERIGRDITLDELEREVLKDDIFFRTSGGGVTLSGGEVLMQAPFATRFLQRLRRWGVPCAIETAGDTSASR
LLPLARACDEVLFDLKIMDAERAREVINMNLPRVLENLRLLVSEGITVIPRLPLIPGFTLNAENLQCALTLLRSLGIKQV
HLLPFHQYGEPKYRLLGKSWMMKDIPAPSVQEIALFREMAEQAGFQVTTGG

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1790389, Length=292, Percent_Identity=78.7671232876712, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI226510931, Length=299, Percent_Identity=33.4448160535117, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787130, Length=272, Percent_Identity=25.7352941176471, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790839, Length=259, Percent_Identity=24.7104247104247, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006638
- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 32694; Mature: 32563

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFAAPRISCEVVGARDEETARIFNIQRYSLNDGQGIRTVVFFKGCPHTCPWCANPESI
CCEECCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCC
SPRIETVRRENKCLRCTPCLRDADECPSGAFERIGRDITLDELEREVLKDDIFFRTSGGG
CHHHHHHHHCCCCEEECCCCCCHHCCCCHHHHHCCCCCCHHHHHHHHHHHCCEEEECCCC
VTLSGGEVLMQAPFATRFLQRLRRWGVPCAIETAGDTSASRLLPLARACDEVLFDLKIMD
EEEECCCEEEECHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEC
AERAREVINMNLPRVLENLRLLVSEGITVIPRLPLIPGFTLNAENLQCALTLLRSLGIKQ
HHHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCEECCCHHHHHHHHHHHCCCCE
VHLLPFHQYGEPKYRLLGKSWMMKDIPAPSVQEIALFREMAEQAGFQVTTGG
EEEEECHHCCCCHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEECCC
>Mature Secondary Structure 
TLFAAPRISCEVVGARDEETARIFNIQRYSLNDGQGIRTVVFFKGCPHTCPWCANPESI
CEECCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCC
SPRIETVRRENKCLRCTPCLRDADECPSGAFERIGRDITLDELEREVLKDDIFFRTSGGG
CHHHHHHHHCCCCEEECCCCCCHHCCCCHHHHHCCCCCCHHHHHHHHHHHCCEEEECCCC
VTLSGGEVLMQAPFATRFLQRLRRWGVPCAIETAGDTSASRLLPLARACDEVLFDLKIMD
EEEECCCEEEECHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEC
AERAREVINMNLPRVLENLRLLVSEGITVIPRLPLIPGFTLNAENLQCALTLLRSLGIKQ
HHHHHHHHCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCEECCCHHHHHHHHHHHCCCCE
VHLLPFHQYGEPKYRLLGKSWMMKDIPAPSVQEIALFREMAEQAGFQVTTGG
EEEEECHHCCCCHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]