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The map label for this gene is pflD [H]

Identifier: 16767379

GI number: 16767379

Start: 4328814

End: 4331111

Strand: Direct

Name: pflD [H]

Synonym: STM4114

Alternate gene names: 16767379

Gene position: 4328814-4331111 (Clockwise)

Preceding gene: 16767378

Following gene: 16767380

Centisome position: 89.12

GC content: 55.66

Gene sequence:

>2298_bases
ATGACCCATCGTATTCAACGCCTCAAAGCCGCTCTGTTTCAGAATCACCGTGAAATTTCTCTGGAGCGCGCGCTGCTTTA
TACCGCCAGCCATCAGCAGACGGAGGGCGAACCGGTCATTTTGCGCCGCGCCAAAGCGACGGCGTATATCCTTGAACATG
TTGAAATTTCGATTCGTGACGAGGAGCTGATTGCCGGAAACCGAACCGTTAAGCCGCGCGCGGGTATTATGTCGCCGGAA
ATGGACCCGTATTGGCTGCTTAACGAGCTGGATCGGTTCCCGACGCGTCCGCAGGACCGTTTTGATATCAGCGAAGAGGA
TAAGCGCCTCTATCGTGAGGTTCTATTCCCGTACTGGGAAAAACGGTCGATGAAAGATTTTATTAACGGGCAGATGACCG
ATGAGGTGAAAGCCGCCGTCAGCACCCAGATTTTTAGCATTAACCAGACGGATAAAGGGCAGGGGCATATCATTATTGAT
TATCCACGCTTGCTCAATAACGGGCTGGGTGAACTGGCGGCGCAGATGCGCGCGCGCTGCGAGCAACAGCCGGAAAACGA
CTTTTATCAGGCCGCTCTGTTGTTACTTGAGGCATCGCAACGCCACATCCTGCGTTATGCCGTACTGGCGGAACAGCAGG
CGGAGCGCTGCCCGGACGCTCGTCGTCGTCAGGAACTGCTCACCATTGCCGCCAATTCGCGGCATAACGCGCAGCACAAA
CCACAGACGTTCTGGCAAGCATGTCAGCTGTTCTGGTACATGAACATTATTTTGCAGTACGAGTCCAACGCCAGTTCGCT
GTCGCTCGGGCGCTTCGACCAGTACATGCTGCCGTTTTATCAGACTTCGCTCACTCAGGGCGACGATCCGGCGTTCCTGA
AAGAGTTGCTCGAGTCGCTGTGGGTGAAATGCAACGATATCGTGCTGCTGCGTTCCACCAGTAGCGCGCGCTATTTTGCC
GGTTTCCCGACGGGTTACACCGCGCTGCTGGGCGGATTGACGGAAAGCGGACGCAGCGCGGTAAACGTGCTCTCTTTTCT
CTGCCTGGATGCCTACCAGAGCGTGCAGTTGCCGCAGCCGAATCTCGGCGTGCGCACTAACGCGCTGATTGATACGCCGT
TCCTGCTCAAGACCGCCGAGACCATTCGCCTCGGTACCGGCATTCCACAGATTTTCAATGACGAAGTGGTGGTTCCCGCG
TTCCTTAACCGTGGCGTGTCGTTGGAGGATGCGCGCGATTATGCGGTGGTGGGATGTGTTGAGCTGTCGATTCCGGGCAG
AACCTACGGGCTGCATGACATCGCCATGTTTAATCTGCTGAAAGTGATGGAAATCAGCTTGTATGAAAATGAGGGCAACG
ACACTCTTACTTATGAGGCGTTGCTGGCGCATATTCGCGCCAAAATCAGTCACTACATTACACTAATGGTAGAAGGCAGC
AATATTTGCGATATCGGCCATCGTGACTGGGCGCCGGTGCCGCTGCTCTCTTCGTTTATCAGCGACTGCCTGGAGAAAGG
GCGAGACATCACCGACGGCGGCGCGCGCTATAACTTTTCGGGCGTACAGGGCATCGGTATCGCCAACCTGAGTGATTCCC
TTCATGCGCTCAATGGGCTGGTATTCGATCAGCAGCGCCTGAGTTTTGATGCGCTGCTGTCGATCCTGAAAAACAACTTC
GCCACGCCAGAAGGGGAAAAGATCCGCGCCAGGCTTATCAACCGCTTCGAAAAATATGGCAACGACATTGATAATGTCGA
TAACATCAGCGCCGAACTGCTGCGGTATTACTGCAAAGAGGTGGAAAAATACCAGAATCCGCGCGGCGGCCAGTTTACGC
CGGGTTCATACACGGTTTCCGCGCATGTGCCGCTGGGAGCGGTGGTCGGCGCGACACCGGACGGGCGTTTTGCCGGAGAA
CAGCTGGCGGATGGCGGCCTGTCGCCGATGCTCGGACAGGATATGCAAGGGCCGACGGCGGTACTAAAATCAGTAAGTAA
GTTGGATAACACGCTGCTGTCGAACGGTACGTTGCTGAACGTGAAGTTTACTCCTGCGACTCTGGAAGGAGAGGCGGGAC
TGCGCAAGCTGGCGGATTTCCTGCGCGCGTTTACCCAGCTCAAACTGCAGCACATCCAGTTTAATGTGGTGAATGCCGAC
ACATTGCGCGAAGCGCAGCTACGGCCTCAGGATTTCGCTGGGCTGGTGGTGCGCGTGGCGGGCTACAGCGCCTTCTTTGT
CGAGCTGTCGAAGGAGATTCAGGATGACATTATTCGCCGCACCGCGCATCAGTTGTGA

Upstream 100 bases:

>100_bases
AACGTTAAAATGCCCGATGGCGCAAGTTTATCGGGCCTGCTCACAGGCCCGATAAGAGATTTATGCCGCCATCCGGCGCT
CGTGTCCACAGGAGCTCCTC

Downstream 100 bases:

>100_bases
GGTTGTGGGGGCGCGCGATGAGGAGACCGCGCGTATCTTCAATATCCAGCGCTATTCGCTGAATGACGGGCAGGGGATTC
GTACGGTGGTCTTTTTTAAG

Product: formate acetyltransferase 2

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 765; Mature: 764

Protein sequence:

>765_residues
MTHRIQRLKAALFQNHREISLERALLYTASHQQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE
MDPYWLLNELDRFPTRPQDRFDISEEDKRLYREVLFPYWEKRSMKDFINGQMTDEVKAAVSTQIFSINQTDKGQGHIIID
YPRLLNNGLGELAAQMRARCEQQPENDFYQAALLLLEASQRHILRYAVLAEQQAERCPDARRRQELLTIAANSRHNAQHK
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQTSLTQGDDPAFLKELLESLWVKCNDIVLLRSTSSARYFA
GFPTGYTALLGGLTESGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLLKTAETIRLGTGIPQIFNDEVVVPA
FLNRGVSLEDARDYAVVGCVELSIPGRTYGLHDIAMFNLLKVMEISLYENEGNDTLTYEALLAHIRAKISHYITLMVEGS
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALNGLVFDQQRLSFDALLSILKNNF
ATPEGEKIRARLINRFEKYGNDIDNVDNISAELLRYYCKEVEKYQNPRGGQFTPGSYTVSAHVPLGAVVGATPDGRFAGE
QLADGGLSPMLGQDMQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
TLREAQLRPQDFAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL

Sequences:

>Translated_765_residues
MTHRIQRLKAALFQNHREISLERALLYTASHQQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE
MDPYWLLNELDRFPTRPQDRFDISEEDKRLYREVLFPYWEKRSMKDFINGQMTDEVKAAVSTQIFSINQTDKGQGHIIID
YPRLLNNGLGELAAQMRARCEQQPENDFYQAALLLLEASQRHILRYAVLAEQQAERCPDARRRQELLTIAANSRHNAQHK
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQTSLTQGDDPAFLKELLESLWVKCNDIVLLRSTSSARYFA
GFPTGYTALLGGLTESGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLLKTAETIRLGTGIPQIFNDEVVVPA
FLNRGVSLEDARDYAVVGCVELSIPGRTYGLHDIAMFNLLKVMEISLYENEGNDTLTYEALLAHIRAKISHYITLMVEGS
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALNGLVFDQQRLSFDALLSILKNNF
ATPEGEKIRARLINRFEKYGNDIDNVDNISAELLRYYCKEVEKYQNPRGGQFTPGSYTVSAHVPLGAVVGATPDGRFAGE
QLADGGLSPMLGQDMQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
TLREAQLRPQDFAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
>Mature_764_residues
THRIQRLKAALFQNHREISLERALLYTASHQQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEM
DPYWLLNELDRFPTRPQDRFDISEEDKRLYREVLFPYWEKRSMKDFINGQMTDEVKAAVSTQIFSINQTDKGQGHIIIDY
PRLLNNGLGELAAQMRARCEQQPENDFYQAALLLLEASQRHILRYAVLAEQQAERCPDARRRQELLTIAANSRHNAQHKP
QTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQTSLTQGDDPAFLKELLESLWVKCNDIVLLRSTSSARYFAG
FPTGYTALLGGLTESGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLLKTAETIRLGTGIPQIFNDEVVVPAF
LNRGVSLEDARDYAVVGCVELSIPGRTYGLHDIAMFNLLKVMEISLYENEGNDTLTYEALLAHIRAKISHYITLMVEGSN
ICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALNGLVFDQQRLSFDALLSILKNNFA
TPEGEKIRARLINRFEKYGNDIDNVDNISAELLRYYCKEVEKYQNPRGGQFTPGSYTVSAHVPLGAVVGATPDGRFAGEQ
LADGGLSPMLGQDMQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADT
LREAQLRPQDFAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=765, Percent_Identity=92.4183006535948, Blast_Score=1453, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=807, Percent_Identity=37.546468401487, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI48994926, Length=643, Percent_Identity=25.194401244168, Blast_Score=142, Evalue=9e-35,
Organism=Escherichia coli, GI1787131, Length=646, Percent_Identity=25.2321981424149, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85987; Mature: 85856

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHRIQRLKAALFQNHREISLERALLYTASHQQTEGEPVILRRAKATAYILEHVEISIRD
CCHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCCEEEEEHHHHHHHHHHEEEEEEC
EELIAGNRTVKPRAGIMSPEMDPYWLLNELDRFPTRPQDRFDISEEDKRLYREVLFPYWE
CCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KRSMKDFINGQMTDEVKAAVSTQIFSINQTDKGQGHIIIDYPRLLNNGLGELAAQMRARC
HCCHHHHHCCCCHHHHHHHHHHHEEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHH
EQQPENDFYQAALLLLEASQRHILRYAVLAEQQAERCPDARRRQELLTIAANSRHNAQHK
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCCC
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQTSLTQGDDPAFLKELLESL
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
WVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLTESGRSAVNVLSFLCLDAYQSVQLPQP
CCCCCCEEEEECCCCCEEEECCCCHHHHHHHCHHHCHHHHHHHHHHHHHHHHHHCCCCCC
NLGVRTNALIDTPFLLKTAETIRLGTGIPQIFNDEVVVPAFLNRGVSLEDARDYAVVGCV
CCCCCCCEEECCCHHHHHHHHHEECCCCCHHHCCCEEEHHHHCCCCCCCCCCCEEEEEEE
ELSIPGRTYGLHDIAMFNLLKVMEISLYENEGNDTLTYEALLAHIRAKISHYITLMVEGS
EEECCCCCCCHHHHHHHHHHHHHHHEEEECCCCCEEHHHHHHHHHHHHHHHEEEEEEECC
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALNGL
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEECCCCHHHHHHHCC
VFDQQRLSFDALLSILKNNFATPEGEKIRARLINRFEKYGNDIDNVDNISAELLRYYCKE
EECHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VEKYQNPRGGQFTPGSYTVSAHVPLGAVVGATPDGRFAGEQLADGGLSPMLGQDMQGPTA
HHHHCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCHHH
VLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECHH
TLREAQLRPQDFAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
HHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
THRIQRLKAALFQNHREISLERALLYTASHQQTEGEPVILRRAKATAYILEHVEISIRD
CHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCCEEEEEHHHHHHHHHHEEEEEEC
EELIAGNRTVKPRAGIMSPEMDPYWLLNELDRFPTRPQDRFDISEEDKRLYREVLFPYWE
CCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KRSMKDFINGQMTDEVKAAVSTQIFSINQTDKGQGHIIIDYPRLLNNGLGELAAQMRARC
HCCHHHHHCCCCHHHHHHHHHHHEEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHH
EQQPENDFYQAALLLLEASQRHILRYAVLAEQQAERCPDARRRQELLTIAANSRHNAQHK
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCCC
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQTSLTQGDDPAFLKELLESL
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
WVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLTESGRSAVNVLSFLCLDAYQSVQLPQP
CCCCCCEEEEECCCCCEEEECCCCHHHHHHHCHHHCHHHHHHHHHHHHHHHHHHCCCCCC
NLGVRTNALIDTPFLLKTAETIRLGTGIPQIFNDEVVVPAFLNRGVSLEDARDYAVVGCV
CCCCCCCEEECCCHHHHHHHHHEECCCCCHHHCCCEEEHHHHCCCCCCCCCCCEEEEEEE
ELSIPGRTYGLHDIAMFNLLKVMEISLYENEGNDTLTYEALLAHIRAKISHYITLMVEGS
EEECCCCCCCHHHHHHHHHHHHHHHEEEECCCCCEEHHHHHHHHHHHHHHHEEEEEEECC
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALNGL
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEECCCCHHHHHHHCC
VFDQQRLSFDALLSILKNNFATPEGEKIRARLINRFEKYGNDIDNVDNISAELLRYYCKE
EECHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VEKYQNPRGGQFTPGSYTVSAHVPLGAVVGATPDGRFAGEQLADGGLSPMLGQDMQGPTA
HHHHCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCHHHHCCCCCCCCCCCCCCHHH
VLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECHH
TLREAQLRPQDFAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
HHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]