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The map label for this gene is est [H]

Identifier: 16767297

GI number: 16767297

Start: 4240489

End: 4241418

Strand: Reverse

Name: est [H]

Synonym: STM4032

Alternate gene names: 16767297

Gene position: 4241418-4240489 (Counterclockwise)

Preceding gene: 16767299

Following gene: 16767296

Centisome position: 87.32

GC content: 55.81

Gene sequence:

>930_bases
ATGCCACTGGAAAAAGGAATTGCCGAACTGGTCGCTGGGTTTATCGCCGCGGGCCGCCCCTCTTCTCGCGAACAGAATAT
TGATGACCGGAGGGCGGGCTACATCGCCAGCACGACACTGGCAGGAGAAACAGAAACCCGCGTCCAGGTTGAAGATATTG
AACTCGATGCCATGACGTTTCGCGTGGTTTCGCCGCTCAATGCCACAGGGAAATTACCCTGCATCATTTATTATCATGGT
GGCTGCTTTGTCAGCGGCGGTTTTGCCACTCACGATAACCAGTTAAGGCAGTTAGCTTTTTACAGCCGTTGTCGCGTGAT
TGCGGCACAGTACCGACTTGCACCAGATCACACCTTCCCGGCCGCACATAACGATGCCGAAACCGGAGCCAATACTATCT
GGAAATATGCCCAGAAACTGGGGATAGATCGCGAGAACATTACGCTTGCTGGTGACAGCGCGGGTGGGCATCTGGCGCTG
GTCACCGCGCTACGCCTGAAAGCCGCCCGTCAATGGCAACCTGCACAGCTGATTCTGATCTACCCCATGCTGGACGCAAC
CGCCAGGTTTGCGAGCTACACCTGTAATGGGCTGGATTACATTATCACGCGTGACACGCTGCTTTCAGGCTACGAGATGT
ATATGCCGCGAACCGATCCACTGCATCCTGAAGCCAGTCCGCTCTGGCGTGAGGACTTCGCCGGACTGCCATCCACGCAC
ATCATTACTGCCGAATTCGACCCACTGCGCGATGAGGGCGAGGCCCTGTATCAACGGTTCCAGGAACAGGGTGTGGAATG
TACTTGCCAGCGCTATCTCGGTGTCATCCATGGCTTTTTCCAGCTCGGAGGCGTGAGCCAGGCGGCACGAAGCGCCATGC
GAGATGTGGCCTGGCGTGTTGTAAGTCCATCAACAGGCAAAATGACCTAA

Upstream 100 bases:

>100_bases
CGAACCTCTATCCAGAATCATTATCCCCTCGTTTCACCACTTTCCATCCCCAACGAACCAGGTTATAAACTGTACATACA
TACAGTCAAAGGAGCGTTAT

Downstream 100 bases:

>100_bases
AATTCAATTTTGTTTTCAACTTGAAAACTGGATAACGTTGCGCTAAATTGTTTTCAGGTTGAAAACTTTGTAATGGAACT
ACATTGTGCATGTTATCAGC

Product: acetyl esterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MPLEKGIAELVAGFIAAGRPSSREQNIDDRRAGYIASTTLAGETETRVQVEDIELDAMTFRVVSPLNATGKLPCIIYYHG
GCFVSGGFATHDNQLRQLAFYSRCRVIAAQYRLAPDHTFPAAHNDAETGANTIWKYAQKLGIDRENITLAGDSAGGHLAL
VTALRLKAARQWQPAQLILIYPMLDATARFASYTCNGLDYIITRDTLLSGYEMYMPRTDPLHPEASPLWREDFAGLPSTH
IITAEFDPLRDEGEALYQRFQEQGVECTCQRYLGVIHGFFQLGGVSQAARSAMRDVAWRVVSPSTGKMT

Sequences:

>Translated_309_residues
MPLEKGIAELVAGFIAAGRPSSREQNIDDRRAGYIASTTLAGETETRVQVEDIELDAMTFRVVSPLNATGKLPCIIYYHG
GCFVSGGFATHDNQLRQLAFYSRCRVIAAQYRLAPDHTFPAAHNDAETGANTIWKYAQKLGIDRENITLAGDSAGGHLAL
VTALRLKAARQWQPAQLILIYPMLDATARFASYTCNGLDYIITRDTLLSGYEMYMPRTDPLHPEASPLWREDFAGLPSTH
IITAEFDPLRDEGEALYQRFQEQGVECTCQRYLGVIHGFFQLGGVSQAARSAMRDVAWRVVSPSTGKMT
>Mature_308_residues
PLEKGIAELVAGFIAAGRPSSREQNIDDRRAGYIASTTLAGETETRVQVEDIELDAMTFRVVSPLNATGKLPCIIYYHGG
CFVSGGFATHDNQLRQLAFYSRCRVIAAQYRLAPDHTFPAAHNDAETGANTIWKYAQKLGIDRENITLAGDSAGGHLALV
TALRLKAARQWQPAQLILIYPMLDATARFASYTCNGLDYIITRDTLLSGYEMYMPRTDPLHPEASPLWREDFAGLPSTHI
ITAEFDPLRDEGEALYQRFQEQGVECTCQRYLGVIHGFFQLGGVSQAARSAMRDVAWRVVSPSTGKMT

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=266, Percent_Identity=27.4436090225564, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786682, Length=226, Percent_Identity=31.4159292035398, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71996133, Length=128, Percent_Identity=37.5, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540028, Length=121, Percent_Identity=33.8842975206612, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI72001146, Length=141, Percent_Identity=32.6241134751773, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34155; Mature: 34024

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLEKGIAELVAGFIAAGRPSSREQNIDDRRAGYIASTTLAGETETRVQVEDIELDAMTF
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEEEECCCCCCEEEEEEEEEEEEEE
RVVSPLNATGKLPCIIYYHGGCFVSGGFATHDNQLRQLAFYSRCRVIAAQYRLAPDHTFP
EEECCCCCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AAHNDAETGANTIWKYAQKLGIDRENITLAGDSAGGHLALVTALRLKAARQWQPAQLILI
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
YPMLDATARFASYTCNGLDYIITRDTLLSGYEMYMPRTDPLHPEASPLWREDFAGLPSTH
EECHHHHHHHHHHCCCCEEEEEEHHHHHCCHHEECCCCCCCCCCCCCCHHHHHCCCCCCE
IITAEFDPLRDEGEALYQRFQEQGVECTCQRYLGVIHGFFQLGGVSQAARSAMRDVAWRV
EEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEE
VSPSTGKMT
ECCCCCCCC
>Mature Secondary Structure 
PLEKGIAELVAGFIAAGRPSSREQNIDDRRAGYIASTTLAGETETRVQVEDIELDAMTF
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEEEECCCCCCEEEEEEEEEEEEEE
RVVSPLNATGKLPCIIYYHGGCFVSGGFATHDNQLRQLAFYSRCRVIAAQYRLAPDHTFP
EEECCCCCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AAHNDAETGANTIWKYAQKLGIDRENITLAGDSAGGHLALVTALRLKAARQWQPAQLILI
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
YPMLDATARFASYTCNGLDYIITRDTLLSGYEMYMPRTDPLHPEASPLWREDFAGLPSTH
EECHHHHHHHHHHCCCCEEEEEEHHHHHCCHHEECCCCCCCCCCCCCCHHHHHCCCCCCE
IITAEFDPLRDEGEALYQRFQEQGVECTCQRYLGVIHGFFQLGGVSQAARSAMRDVAWRV
EEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEE
VSPSTGKMT
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]