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The map label for this gene is rfaL

Identifier: 16766998

GI number: 16766998

Start: 3908290

End: 3909504

Strand: Direct

Name: rfaL

Synonym: STM3713

Alternate gene names: 16766998

Gene position: 3908290-3909504 (Clockwise)

Preceding gene: 16766997

Following gene: 16767009

Centisome position: 80.46

GC content: 39.26

Gene sequence:

>1215_bases
ATGCTAACCACATCATTAACGTTAAATAAAGAGAAATGGAAGCCGATCTGGAATAAAGCGCTGGTTTTTCTTTTTGTTGC
CACGTATTTTCTGGATGGTATTACGCGTTATAAACATTTGATAATCATACTTATGGTTATCACCGCGATTTATCAGGTCT
CACGCTCACCGAAAAGTTTCCCCCCTCTTTTCAAAAATAGCGTATTTTATAGCGTAGCAGTATTATCATTAATCCTTGTT
TATTCCATACTCATATCGCCAGATATGAAAGAAAGTTTCAAGGAATTTGAAAATACGGTACTGGAGGGCTTCTTATTATA
TACTTTATTAATTCCCGTACTATTAAAAGATGAAACAAAAGAAACGGTTGCGAAAATAGTACTTTTCTCCTTTTTAACAA
GTTTAGGACTTCGCTGCCTTGCAGAGAGTATTCTGTATATCGAGGACTATAATAAAGGGATTATGCCATTCATAAGCTAT
GCGCATCGACATATGTCCGATTCCATGGTTTTCTTATTTCCAGCATTATTGAATATTTGGCTGTTTAGAAAAAATGCAAT
TAAGTTGGTTTTTTTGGTGCTTAGCGCCATCTACCTTTTCTTTATCCTGGGAACCCTATCGCGAGGGGCATGGTTGGCGG
TGCTTATAGTAGGTGTTCTGTGGGCAATACTGAACCGCCAATGGAAGTTAATAGGAGTTGGTGCCATTTTATTAGCCATT
ATCGGCGCTTTGGTTATCACTCAACATAATAACAAACCAGACCCAGAACATTTACTGTATAAATTACAGCAGACAGATAG
CTCATATCGTTATACTAACGGAACCCAGGGCACCGCGTGGATACTGATTCAGGAAAACCCGATCAAGGGCTACGGCTATG
GTAATGATGTGTATGATGGTGTTTATAATAAACGCGTTGTCGATTATCCAACGTGGACCTTTAAAGAATCTATCGGTCCG
CATAATACCATTCTGTACATCTGGTTTAGTGCAGGCATATTGGGTCTGGCGAGCCTGGTCTATTTATATGGCGCTATCAT
CAGGGAAACAGCCAGCTCTACCCTCAGGAAAGTAGAGATAAGCCCCTACAATGCTCATCTCTTGCTATTTTTATCTTTCG
TCGGTTTTTATATCGTTCGTGGCAATTTTGAACAGGTCGATATTGCTCAAATTGGTATCATTACCGGTTTTCTGCTGGCG
CTAAGAAATAGATAA

Upstream 100 bases:

>100_bases
CTCACCAGAACAGAACCTGGCGAATTTAGATGCCACAAGCGTATTTGGAAAGATTCATTAAAGAGACTCTGTCTCATCCC
AAACCTATTGTGGAGAAAAG

Downstream 100 bases:

>100_bases
AAAAACGCGCTGATACTTATTACGGTATCAGCGCGTTTTCCATCATCAGGACTCAATCACTTATCAAACCAGTTTTTCAT
TTGTTCCTCGAAACGCTGCG

Product: O-antigen ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MLTTSLTLNKEKWKPIWNKALVFLFVATYFLDGITRYKHLIIILMVITAIYQVSRSPKSFPPLFKNSVFYSVAVLSLILV
YSILISPDMKESFKEFENTVLEGFLLYTLLIPVLLKDETKETVAKIVLFSFLTSLGLRCLAESILYIEDYNKGIMPFISY
AHRHMSDSMVFLFPALLNIWLFRKNAIKLVFLVLSAIYLFFILGTLSRGAWLAVLIVGVLWAILNRQWKLIGVGAILLAI
IGALVITQHNNKPDPEHLLYKLQQTDSSYRYTNGTQGTAWILIQENPIKGYGYGNDVYDGVYNKRVVDYPTWTFKESIGP
HNTILYIWFSAGILGLASLVYLYGAIIRETASSTLRKVEISPYNAHLLLFLSFVGFYIVRGNFEQVDIAQIGIITGFLLA
LRNR

Sequences:

>Translated_404_residues
MLTTSLTLNKEKWKPIWNKALVFLFVATYFLDGITRYKHLIIILMVITAIYQVSRSPKSFPPLFKNSVFYSVAVLSLILV
YSILISPDMKESFKEFENTVLEGFLLYTLLIPVLLKDETKETVAKIVLFSFLTSLGLRCLAESILYIEDYNKGIMPFISY
AHRHMSDSMVFLFPALLNIWLFRKNAIKLVFLVLSAIYLFFILGTLSRGAWLAVLIVGVLWAILNRQWKLIGVGAILLAI
IGALVITQHNNKPDPEHLLYKLQQTDSSYRYTNGTQGTAWILIQENPIKGYGYGNDVYDGVYNKRVVDYPTWTFKESIGP
HNTILYIWFSAGILGLASLVYLYGAIIRETASSTLRKVEISPYNAHLLLFLSFVGFYIVRGNFEQVDIAQIGIITGFLLA
LRNR
>Mature_404_residues
MLTTSLTLNKEKWKPIWNKALVFLFVATYFLDGITRYKHLIIILMVITAIYQVSRSPKSFPPLFKNSVFYSVAVLSLILV
YSILISPDMKESFKEFENTVLEGFLLYTLLIPVLLKDETKETVAKIVLFSFLTSLGLRCLAESILYIEDYNKGIMPFISY
AHRHMSDSMVFLFPALLNIWLFRKNAIKLVFLVLSAIYLFFILGTLSRGAWLAVLIVGVLWAILNRQWKLIGVGAILLAI
IGALVITQHNNKPDPEHLLYKLQQTDSSYRYTNGTQGTAWILIQENPIKGYGYGNDVYDGVYNKRVVDYPTWTFKESIGP
HNTILYIWFSAGILGLASLVYLYGAIIRETASSTLRKVEISPYNAHLLLFLSFVGFYIVRGNFEQVDIAQIGIITGFLLA
LRNR

Specific function: Adds the O-antigen on the glucose(II) group of LPS

COG id: COG3307

COG function: function code M; Lipid A core - O-antigen ligase and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the O-antigen polymerase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFAL_SALTY (P26471)

Other databases:

- EMBL:   M73826
- EMBL:   AE006468
- PIR:   B41317
- RefSeq:   NP_462613.1
- PRIDE:   P26471
- GeneID:   1255237
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM3713
- NMPDR:   fig|99287.1.peg.3589
- HOGENOM:   HBG467985
- OMA:   NDVYDSV
- ProtClustDB:   PRK15487
- BioCyc:   STYP99287:STM3713-MONOMER
- InterPro:   IPR007016

Pfam domain/function: PF04932 Wzy_C

EC number: 6.-.-.-

Molecular weight: Translated: 46035; Mature: 46035

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1172c53c)-; HASH(0x123df9a0)-; HASH(0x4099c1bc)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTSLTLNKEKWKPIWNKALVFLFVATYFLDGITRYKHLIIILMVITAIYQVSRSPKSF
CCCCCEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PPLFKNSVFYSVAVLSLILVYSILISPDMKESFKEFENTVLEGFLLYTLLIPVLLKDETK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ETVAKIVLFSFLTSLGLRCLAESILYIEDYNKGIMPFISYAHRHMSDSMVFLFPALLNIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
LFRKNAIKLVFLVLSAIYLFFILGTLSRGAWLAVLIVGVLWAILNRQWKLIGVGAILLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHH
IGALVITQHNNKPDPEHLLYKLQQTDSSYRYTNGTQGTAWILIQENPIKGYGYGNDVYDG
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHH
VYNKRVVDYPTWTFKESIGPHNTILYIWFSAGILGLASLVYLYGAIIRETASSTLRKVEI
HHCCEEECCCCCHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
SPYNAHLLLFLSFVGFYIVRGNFEQVDIAQIGIITGFLLALRNR
CCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MLTTSLTLNKEKWKPIWNKALVFLFVATYFLDGITRYKHLIIILMVITAIYQVSRSPKSF
CCCCCEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PPLFKNSVFYSVAVLSLILVYSILISPDMKESFKEFENTVLEGFLLYTLLIPVLLKDETK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ETVAKIVLFSFLTSLGLRCLAESILYIEDYNKGIMPFISYAHRHMSDSMVFLFPALLNIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
LFRKNAIKLVFLVLSAIYLFFILGTLSRGAWLAVLIVGVLWAILNRQWKLIGVGAILLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHH
IGALVITQHNNKPDPEHLLYKLQQTDSSYRYTNGTQGTAWILIQENPIKGYGYGNDVYDG
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHH
VYNKRVVDYPTWTFKESIGPHNTILYIWFSAGILGLASLVYLYGAIIRETASSTLRKVEI
HHCCEEECCCCCHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
SPYNAHLLLFLSFVGFYIVRGNFEQVDIAQIGIITGFLLALRNR
CCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1657881; 11677609