Click here to switch to the map view.

The map label for this gene is rfaC

Identifier: 16766997

GI number: 16766997

Start: 3907297

End: 3908250

Strand: Direct

Name: rfaC

Synonym: STM3712

Alternate gene names: 16766997

Gene position: 3907297-3908250 (Clockwise)

Preceding gene: 16766996

Following gene: 16766998

Centisome position: 80.44

GC content: 55.35

Gene sequence:

>954_bases
ATGCGGGTTTTGATCGTTAAAACATCATCGATGGGCGACGTATTACATACCCTGCCTGCGCTTACCGACGCGCAACAGGC
GATTCCGGGGATTCAATTTGATTGGGCTGTCGAAGAAGGGTTTGCACAAATTCCGTCCTGGCACAGTGCTGTCGATCGCG
TGATTCCCGTCGCTATTCGCCGTTGGCGCAAGGCCTGGTTTTCCGCGCCCATCAAAGCGGAACGCACAGCCTTTCGTCGG
GCGGTATGCGCAAACCAATACGACGCTGTGATTGATGCGCAGGGGCTGGTAAAAAGCGCGGCGCTGGTGACGCGTCTGGC
GCATGGGATAAAGCACGGTATGGACTGGAGTACCGCCCGCGAACCGCTGGCCAGCCTGTTCTATAACCGTAAACACCATA
TCGCAAAGCAACAACATGCGGTTGAACGGACGCGCGAGCTGTTCGCCAAAAGCCTGGGATACGATAAACCGCAGTCGCAG
GGCGATTATGCCATCGCAAAACATTTTCTGCATTGCCAGCAGGCGGTTAGCGATCCGTATGCGGTGTTTTTACATGCCAC
GACCCGCGATGATAAACACTGGCCGGAAGCAAACTGGCGCGAGCTTATCGGCCTGGTGGGCAACACCGGATTACGGATAA
AGCTTCCCTGGGGCGCGCCTCATGAGGAGGCCCGGGCTAAACGACTGGCCGAAGGCTTTGACTATGTGGATGTGTTACCG
CGCATGAGCCTGGAGGAGGTCGCCAGAGTGCTGGCTGGCGCAAAATTTGTCGTATCGGTTGATACCGGCCTGAGCCATCT
CACCGCCGCGCTCGACAGACCGAATATTACGCTATATGGCCCAACGGACCCTGGGTTAATTGGAGGTTATGGGAAGAACC
AAATGGCATGCTGCTCACCAGAACAGAACCTGGCGAATTTAGATGCCACAAGCGTATTTGGAAAGATTCATTAA

Upstream 100 bases:

>100_bases
AAGGTGATACGGCGCAAGGCTATCACCAGAGCCTGATCGATATCACGCCGCAGCGGGTTCTGGAAGAGCTTCATTCGCTG
TTGTCGGAAGAGGGCGTTTA

Downstream 100 bases:

>100_bases
AGAGACTCTGTCTCATCCCAAACCTATTGTGGAGAAAAGATGCTAACCACATCATTAACGTTAAATAAAGAGAAATGGAA
GCCGATCTGGAATAAAGCGC

Product: ADP-heptose:LPS heptosyl transferase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIQFDWAVEEGFAQIPSWHSAVDRVIPVAIRRWRKAWFSAPIKAERTAFRR
AVCANQYDAVIDAQGLVKSAALVTRLAHGIKHGMDWSTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYDKPQSQ
GDYAIAKHFLHCQQAVSDPYAVFLHATTRDDKHWPEANWRELIGLVGNTGLRIKLPWGAPHEEARAKRLAEGFDYVDVLP
RMSLEEVARVLAGAKFVVSVDTGLSHLTAALDRPNITLYGPTDPGLIGGYGKNQMACCSPEQNLANLDATSVFGKIH

Sequences:

>Translated_317_residues
MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIQFDWAVEEGFAQIPSWHSAVDRVIPVAIRRWRKAWFSAPIKAERTAFRR
AVCANQYDAVIDAQGLVKSAALVTRLAHGIKHGMDWSTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYDKPQSQ
GDYAIAKHFLHCQQAVSDPYAVFLHATTRDDKHWPEANWRELIGLVGNTGLRIKLPWGAPHEEARAKRLAEGFDYVDVLP
RMSLEEVARVLAGAKFVVSVDTGLSHLTAALDRPNITLYGPTDPGLIGGYGKNQMACCSPEQNLANLDATSVFGKIH
>Mature_317_residues
MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIQFDWAVEEGFAQIPSWHSAVDRVIPVAIRRWRKAWFSAPIKAERTAFRR
AVCANQYDAVIDAQGLVKSAALVTRLAHGIKHGMDWSTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYDKPQSQ
GDYAIAKHFLHCQQAVSDPYAVFLHATTRDDKHWPEANWRELIGLVGNTGLRIKLPWGAPHEEARAKRLAEGFDYVDVLP
RMSLEEVARVLAGAKFVVSVDTGLSHLTAALDRPNITLYGPTDPGLIGGYGKNQMACCSPEQNLANLDATSVFGKIH

Specific function: Heptose transfer to the lipopolysaccharide core. It transfers the innnermost heptose to [4'-P](3-deoxy-D-manno- octulosonic acid)2-IVA

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family

Homologues:

Organism=Escherichia coli, GI1790051, Length=316, Percent_Identity=84.1772151898734, Blast_Score=556, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFAC_SALTY (P26469)

Other databases:

- EMBL:   M95927
- EMBL:   AE006468
- EMBL:   M73826
- PIR:   A44156
- RefSeq:   NP_462612.1
- ProteinModelPortal:   P26469
- SMR:   P26469
- PRIDE:   P26469
- GeneID:   1255236
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM3712
- NMPDR:   fig|99287.1.peg.3588
- HOGENOM:   HBG489094
- OMA:   GEWRRFK
- ProtClustDB:   PRK10964
- BioCyc:   STYP99287:STM3712-MONOMER
- InterPro:   IPR002201
- InterPro:   IPR011908
- TIGRFAMs:   TIGR02193

Pfam domain/function: PF01075 Glyco_transf_9

EC number: 2.4.1.-

Molecular weight: Translated: 35173; Mature: 35173

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIQFDWAVEEGFAQIPSWHSAVDRVIPVAIR
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHH
RWRKAWFSAPIKAERTAFRRAVCANQYDAVIDAQGLVKSAALVTRLAHGIKHGMDWSTAR
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYDKPQSQGDYAIAKHFLHCQQAVSDPY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCE
AVFLHATTRDDKHWPEANWRELIGLVGNTGLRIKLPWGAPHEEARAKRLAEGFDYVDVLP
EEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHC
RMSLEEVARVLAGAKFVVSVDTGLSHLTAALDRPNITLYGPTDPGLIGGYGKNQMACCSP
CCCHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEECCC
EQNLANLDATSVFGKIH
CCHHHCCCHHHHHCCCC
>Mature Secondary Structure
MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIQFDWAVEEGFAQIPSWHSAVDRVIPVAIR
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHH
RWRKAWFSAPIKAERTAFRRAVCANQYDAVIDAQGLVKSAALVTRLAHGIKHGMDWSTAR
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYDKPQSQGDYAIAKHFLHCQQAVSDPY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCE
AVFLHATTRDDKHWPEANWRELIGLVGNTGLRIKLPWGAPHEEARAKRLAEGFDYVDVLP
EEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHC
RMSLEEVARVLAGAKFVVSVDTGLSHLTAALDRPNITLYGPTDPGLIGGYGKNQMACCSP
CCCHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEECCC
EQNLANLDATSVFGKIH
CCHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1527014; 11677609; 1657881