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The map label for this gene is ansB [H]

Identifier: 16766884

GI number: 16766884

Start: 3769992

End: 3771035

Strand: Reverse

Name: ansB [H]

Synonym: STM3598

Alternate gene names: 16766884

Gene position: 3771035-3769992 (Counterclockwise)

Preceding gene: 16766885

Following gene: 16766881

Centisome position: 77.63

GC content: 51.34

Gene sequence:

>1044_bases
ATGAAAATTAGAGTTTTCATGGCCACCGTGTTGCTGCTCATCAGCCACTGTGTATTTAGCACAACGTCACTACCGCATAT
TGTTATTCTCGCGACAGGTGGTACTATCGCCGGGACGGCAGCCAATAATACGCAAACCGCCGGATATAAATCTGGTGAAC
TTGGCGTGCAAACATTAATAAATGCCGTGCCGGAAATGAATAATATCGCTCGCGTTGACGGCGAGCAGGTGGCGAATATT
GGTAGCGAAAATATGACCAGCGATATCATCCTGAAACTTTCACAGAAGGTGAATGCGTTATTGGCGCGGGACGATGTTGA
CGGTGTGGTTATTACTCATGGCACTGACACGCTCGATGAAACCGCCTACTTTCTTAATTTGACCGTGAAAAGCGACAAAC
CGGTGGTGTTTACCGCTGCAATGCGGCCCGCGTCGGCAATCAGCGCCGATGGCGCAATGAACCTGCTGGAAGCGGTCACG
GTGGCTGCTGACCCGAATGCGAAGGGACGCGGTGTGATGGTGGTTTTAAACGATCGTATTGGTTCGGCGCGCTTTGTGAC
GAAAACTAATGCCACGACTCTGGATACCTTTAAAGCGCCGGAAGAGGGCTATCTGGGGGTCATCGTTAATGGTCAGCCAC
AGTTCGAAACGCGGGTGGAAAAAATTCATACCCTGCGATCTGTTTTTGACGTACGTAATATCAAAAAATTACCCAATGTG
GTGATTATTTACGGCTATCAGGACGACCCGGAATATATGTATGATGCGGCGATCGCCCATCACGCGGACGGTATTATTTA
TGCCGGAACCGGCGCAGGTTCGGTCTCGGTACGCAGCGACGCGGGGATTAAAAAAGCGGAGAAAGCCGGGATTATCGTGG
TGCGCGCTTCCCGCACCGGAAACGGCGTCGTACCGTTGGATAAAGGGCAGCCAGGGCTGGTGTCTGACTCGCTCAACCCG
GCGAAGGCGCGAGTCTTGCTGATGACGGCATTAACTCAGACGCGTAATCCGGAACTGATCCAGAGTTATTTCAGTACGTA
TTAA

Upstream 100 bases:

>100_bases
GTCATTGTCGGGCTGTTAATAGGAAAAATGGTTCTGGCCTGATTGTTATTCATGTTTTTATAGCACACGCCACTTATTAT
TTTTAATTCAGAGGAAGAAA

Downstream 100 bases:

>100_bases
TCATGCAGGCTTGTGCGCCCACCCTTGAAGATGGGCGCACAAGATATTAACGCATGGTCACAAACTCTTCCGATGCGGTC
GGGTGAATAGCGACGGTATT

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKIRVFMATVLLLISHCVFSTTSLPHIVILATGGTIAGTAANNTQTAGYKSGELGVQTLINAVPEMNNIARVDGEQVANI
GSENMTSDIILKLSQKVNALLARDDVDGVVITHGTDTLDETAYFLNLTVKSDKPVVFTAAMRPASAISADGAMNLLEAVT
VAADPNAKGRGVMVVLNDRIGSARFVTKTNATTLDTFKAPEEGYLGVIVNGQPQFETRVEKIHTLRSVFDVRNIKKLPNV
VIIYGYQDDPEYMYDAAIAHHADGIIYAGTGAGSVSVRSDAGIKKAEKAGIIVVRASRTGNGVVPLDKGQPGLVSDSLNP
AKARVLLMTALTQTRNPELIQSYFSTY

Sequences:

>Translated_347_residues
MKIRVFMATVLLLISHCVFSTTSLPHIVILATGGTIAGTAANNTQTAGYKSGELGVQTLINAVPEMNNIARVDGEQVANI
GSENMTSDIILKLSQKVNALLARDDVDGVVITHGTDTLDETAYFLNLTVKSDKPVVFTAAMRPASAISADGAMNLLEAVT
VAADPNAKGRGVMVVLNDRIGSARFVTKTNATTLDTFKAPEEGYLGVIVNGQPQFETRVEKIHTLRSVFDVRNIKKLPNV
VIIYGYQDDPEYMYDAAIAHHADGIIYAGTGAGSVSVRSDAGIKKAEKAGIIVVRASRTGNGVVPLDKGQPGLVSDSLNP
AKARVLLMTALTQTRNPELIQSYFSTY
>Mature_347_residues
MKIRVFMATVLLLISHCVFSTTSLPHIVILATGGTIAGTAANNTQTAGYKSGELGVQTLINAVPEMNNIARVDGEQVANI
GSENMTSDIILKLSQKVNALLARDDVDGVVITHGTDTLDETAYFLNLTVKSDKPVVFTAAMRPASAISADGAMNLLEAVT
VAADPNAKGRGVMVVLNDRIGSARFVTKTNATTLDTFKAPEEGYLGVIVNGQPQFETRVEKIHTLRSVFDVRNIKKLPNV
VIIYGYQDDPEYMYDAAIAHHADGIIYAGTGAGSVSVRSDAGIKKAEKAGIIVVRASRTGNGVVPLDKGQPGLVSDSLNP
AKARVLLMTALTQTRNPELIQSYFSTY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=330, Percent_Identity=46.6666666666667, Blast_Score=254, Evalue=4e-69,
Organism=Escherichia coli, GI1788065, Length=178, Percent_Identity=30.8988764044944, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323189, Length=330, Percent_Identity=43.3333333333333, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6323187, Length=330, Percent_Identity=43.3333333333333, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6323186, Length=330, Percent_Identity=43.3333333333333, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6323184, Length=330, Percent_Identity=43.3333333333333, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6320527, Length=334, Percent_Identity=38.0239520958084, Blast_Score=220, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36935; Mature: 36935

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRVFMATVLLLISHCVFSTTSLPHIVILATGGTIAGTAANNTQTAGYKSGELGVQTLI
CEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEECCCCCCCCCCCCCCCHHHHHHH
NAVPEMNNIARVDGEQVANIGSENMTSDIILKLSQKVNALLARDDVDGVVITHGTDTLDE
HHCCCCCCHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCC
TAYFLNLTVKSDKPVVFTAAMRPASAISADGAMNLLEAVTVAADPNAKGRGVMVVLNDRI
CEEEEEEEEECCCCEEEEECCCCCHHHCCCHHHHHHHHEEEECCCCCCCCEEEEEEECCC
GSARFVTKTNATTLDTFKAPEEGYLGVIVNGQPQFETRVEKIHTLRSVFDVRNIKKLPNV
CCEEEEEECCCCEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
VIIYGYQDDPEYMYDAAIAHHADGIIYAGTGAGSVSVRSDAGIKKAEKAGIIVVRASRTG
EEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCCCCCEEEEEECCCC
NGVVPLDKGQPGLVSDSLNPAKARVLLMTALTQTRNPELIQSYFSTY
CCEEECCCCCCCCCCCCCCHHHHHEEEEEHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKIRVFMATVLLLISHCVFSTTSLPHIVILATGGTIAGTAANNTQTAGYKSGELGVQTLI
CEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEECCCCCCCCCCCCCCCHHHHHHH
NAVPEMNNIARVDGEQVANIGSENMTSDIILKLSQKVNALLARDDVDGVVITHGTDTLDE
HHCCCCCCHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCC
TAYFLNLTVKSDKPVVFTAAMRPASAISADGAMNLLEAVTVAADPNAKGRGVMVVLNDRI
CEEEEEEEEECCCCEEEEECCCCCHHHCCCHHHHHHHHEEEECCCCCCCCEEEEEEECCC
GSARFVTKTNATTLDTFKAPEEGYLGVIVNGQPQFETRVEKIHTLRSVFDVRNIKKLPNV
CCEEEEEECCCCEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
VIIYGYQDDPEYMYDAAIAHHADGIIYAGTGAGSVSVRSDAGIKKAEKAGIIVVRASRTG
EEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCCCCCEEEEEECCCC
NGVVPLDKGQPGLVSDSLNPAKARVLLMTALTQTRNPELIQSYFSTY
CCEEECCCCCCCCCCCCCCHHHHHEEEEEHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3026924; 3194219; 3379033; 8348975 [H]