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The map label for this gene is gldA [H]

Identifier: 16766816

GI number: 16766816

Start: 3692494

End: 3693603

Strand: Reverse

Name: gldA [H]

Synonym: STM3529

Alternate gene names: 16766816

Gene position: 3693603-3692494 (Counterclockwise)

Preceding gene: 16766819

Following gene: 16766815

Centisome position: 76.04

GC content: 54.86

Gene sequence:

>1110_bases
ATGGTTACTACCGCGATCTTTCCGTCACGATATGTTCAGGGTAAAGGAGCGTTGACAACGCATCTCCCGCAGGAATTGGC
CGCACTCGGTCATAAGGCGCTGATTTTGCAGGATCCTGTTGTTCACAACACCTACCGGGATACAGTTGCAACAGCACTGC
ACGGGGTGATTGAATTTGATATCGAAGTGTTCAGTAGCGAGTGCTGTGATGAGGAAATTGCTCGCATCTCCGCACGCGCG
CAGGAAATTGGCGCCGATGTGATTGTTGGTATGGGGGGCGGGAAAACCCTGGATACGGCAAAAGCAACCGGCGCCAGCCT
GCGGCTGCCTATTGCCGTGGTTCCTACTCTGGCCTCAACGGATGCGCCGTGCAGTTCACTGGTTGTTATCTATACGCCAG
AAGGGAAATTCAAACGCTACCTGATGATTCCACGTAACCCGACGCTTGTGCTGGTGGACAGCAGTATTATTGCTGCCGCG
CCGGTACGCTTTTTGGTTTCCGGGATAGGCGATGCGCTGGCAACCTGGTTTGAAGCGGAAGATTGCCGCATCAAAGGGGC
CGGCAATATGACCACCAGGCCAGGACCGATGACCGCATTTGAGCTGGCGCGCTTTTGCTACACCACCTTAATGCGCTATG
GACGCCTGGCGAAACTTGCCTGTGAACAACATCAGGTTACTCCGGCGCTCGAACATGTTATCGAAGCGAATACGCTATTA
TCCGGGCTTGGTTTTGAAAGCGGTGGGCTGGCGGCGGCGCACGCCATTCATAACGGTCTGACAGTACTGCCCGCCACGCA
CAAATACTGGCATGGGGAAAAGGTTGCGTTTGGCACGCTGGCAATGCTTATGCTCACCGATAGAGCGCCTGAGCTTATCG
AGGAGGTTTATCAATTTTGTGAAGATATAGGCTTGCCCACCACGCTGGCGGATATTGGTCTGGCTGGCGTTAGCGATGAT
GAACTGCTGGCCGTTGCCAGGGCGTCTTGTCAAACTGGGGAAACGATACACAACGAGCCGTTTACCATTACGCCGGAGGC
TGTGCAGGCGGCATTGCGGGCCGCCGATGCTGTTGGGCAGGCGCGGAAAAAGATCGATCTTGCAGGTTAA

Upstream 100 bases:

>100_bases
TCTCACCTGGTCATTGTGCGTTCAGAGTAAACAGCATTAAATTCTTTATGTGGAATTTGTGTTCTGTATATTGAACTTTA
ATGCTTTTTAAGGAGAAACA

Downstream 100 bases:

>100_bases
TGTGAGCCTGTAAATAAAAAACGGCAGCCGGAACTTTCAACTTCTGTCTGCCGCTATCGTGAATGTTTTAACGTCGCGAT
TTCAGAAAAATGCATGGGTG

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MVTTAIFPSRYVQGKGALTTHLPQELAALGHKALILQDPVVHNTYRDTVATALHGVIEFDIEVFSSECCDEEIARISARA
QEIGADVIVGMGGGKTLDTAKATGASLRLPIAVVPTLASTDAPCSSLVVIYTPEGKFKRYLMIPRNPTLVLVDSSIIAAA
PVRFLVSGIGDALATWFEAEDCRIKGAGNMTTRPGPMTAFELARFCYTTLMRYGRLAKLACEQHQVTPALEHVIEANTLL
SGLGFESGGLAAAHAIHNGLTVLPATHKYWHGEKVAFGTLAMLMLTDRAPELIEEVYQFCEDIGLPTTLADIGLAGVSDD
ELLAVARASCQTGETIHNEPFTITPEAVQAALRAADAVGQARKKIDLAG

Sequences:

>Translated_369_residues
MVTTAIFPSRYVQGKGALTTHLPQELAALGHKALILQDPVVHNTYRDTVATALHGVIEFDIEVFSSECCDEEIARISARA
QEIGADVIVGMGGGKTLDTAKATGASLRLPIAVVPTLASTDAPCSSLVVIYTPEGKFKRYLMIPRNPTLVLVDSSIIAAA
PVRFLVSGIGDALATWFEAEDCRIKGAGNMTTRPGPMTAFELARFCYTTLMRYGRLAKLACEQHQVTPALEHVIEANTLL
SGLGFESGGLAAAHAIHNGLTVLPATHKYWHGEKVAFGTLAMLMLTDRAPELIEEVYQFCEDIGLPTTLADIGLAGVSDD
ELLAVARASCQTGETIHNEPFTITPEAVQAALRAADAVGQARKKIDLAG
>Mature_369_residues
MVTTAIFPSRYVQGKGALTTHLPQELAALGHKALILQDPVVHNTYRDTVATALHGVIEFDIEVFSSECCDEEIARISARA
QEIGADVIVGMGGGKTLDTAKATGASLRLPIAVVPTLASTDAPCSSLVVIYTPEGKFKRYLMIPRNPTLVLVDSSIIAAA
PVRFLVSGIGDALATWFEAEDCRIKGAGNMTTRPGPMTAFELARFCYTTLMRYGRLAKLACEQHQVTPALEHVIEANTLL
SGLGFESGGLAAAHAIHNGLTVLPATHKYWHGEKVAFGTLAMLMLTDRAPELIEEVYQFCEDIGLPTTLADIGLAGVSDD
ELLAVARASCQTGETIHNEPFTITPEAVQAALRAADAVGQARKKIDLAG

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions [H]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=354, Percent_Identity=49.7175141242938, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI1786815, Length=308, Percent_Identity=32.4675324675325, Blast_Score=135, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 39280; Mature: 39280

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTTAIFPSRYVQGKGALTTHLPQELAALGHKALILQDPVVHNTYRDTVATALHGVIEFD
CCEEEECCCHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
IEVFSSECCDEEIARISARAQEIGADVIVGMGGGKTLDTAKATGASLRLPIAVVPTLAST
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCCEEECEEEECCCCCC
DAPCSSLVVIYTPEGKFKRYLMIPRNPTLVLVDSSIIAAAPVRFLVSGIGDALATWFEAE
CCCCCCEEEEECCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
DCRIKGAGNMTTRPGPMTAFELARFCYTTLMRYGRLAKLACEQHQVTPALEHVIEANTLL
CCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
SGLGFESGGLAAAHAIHNGLTVLPATHKYWHGEKVAFGTLAMLMLTDRAPELIEEVYQFC
HHCCCCCCCHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
EDIGLPTTLADIGLAGVSDDELLAVARASCQTGETIHNEPFTITPEAVQAALRAADAVGQ
HHCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHH
ARKKIDLAG
HHHHCCCCC
>Mature Secondary Structure
MVTTAIFPSRYVQGKGALTTHLPQELAALGHKALILQDPVVHNTYRDTVATALHGVIEFD
CCEEEECCCHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
IEVFSSECCDEEIARISARAQEIGADVIVGMGGGKTLDTAKATGASLRLPIAVVPTLAST
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCCEEECEEEECCCCCC
DAPCSSLVVIYTPEGKFKRYLMIPRNPTLVLVDSSIIAAAPVRFLVSGIGDALATWFEAE
CCCCCCEEEEECCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
DCRIKGAGNMTTRPGPMTAFELARFCYTTLMRYGRLAKLACEQHQVTPALEHVIEANTLL
CCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
SGLGFESGGLAAAHAIHNGLTVLPATHKYWHGEKVAFGTLAMLMLTDRAPELIEEVYQFC
HHCCCCCCCHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
EDIGLPTTLADIGLAGVSDDELLAVARASCQTGETIHNEPFTITPEAVQAALRAADAVGQ
HHCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHH
ARKKIDLAG
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571; 12193646 [H]