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The map label for this gene is asrC

Identifier: 16765870

GI number: 16765870

Start: 2688319

End: 2689332

Strand: Direct

Name: asrC

Synonym: STM2550

Alternate gene names: 16765870

Gene position: 2688319-2689332 (Clockwise)

Preceding gene: 16765869

Following gene: 16765873

Centisome position: 55.34

GC content: 55.92

Gene sequence:

>1014_bases
ATGAGCATTGATATTGATATCATTAAAGCCCGTGCGAAAAACGAATATCGCCTGTCAAAAGTGCGCGGCGAAGCCATGAT
CAGCGTCCGCATTCCCGGCGGTATTTTGCCCGCGCATTTGCTAACGGTGGCGCGTGACATCGCCGAAACCTGGGGCAACG
GACAAATTCACCTCACTACCCGCCAGAAGCTGGCGATGCCGGGTATCCGTTACGAAGATATCGACAACGTGAACGCTGCG
CTGGAGCCGTTTCTTCGCGAGATTGAAATAGAACTGTGCGACGTCCAGGTTGAGGATACCAAAGCGGGCTATCTCGCTAT
TGGCGGGCGAAATATTGTCGCCTGTCAGGGGAACCGCATTTGCCAGAAAGCCAACACTGACACGACCGGTCTGTCCCGTC
GTCTTGAAAAACTGGTCTATCCCAGCCCTTATCATCTCAAAACGGTCATCGTCGGCTGCCCGAATGATTGCGCGAAGGCG
TCAATGGCCGATCTGGGGATTATTGGCGTGGCGAAAATGCGCTTCACTGCCGATCGCTGTATCGGTTGCGGCGCCTGCGT
GAAGGCCTGTAGTCACCACGCGGTAGGCTGCCTGGCGCTGAAGAACGGCAAAGCGGTCAAAGAAGAGTCCGCCTGTATCG
GCTGCGGCGAGTGCGTGCTGGCCTGTCCGACGCTGGCCTGGCAACGTAAGCCGGATCAGCTCTGGCAAGTCCGTCTGGGC
GGACGCACCAGTAAAAAGACGCCGCGCGTCGGCAAGCTCTTCCTCAACTGGGTGACTGAAGATGTGATAAAGCAGGTGAT
CGTTAACCTGTATGAATTTGAAAAAGAGATGCTCGGCGGAAAACCGATCTATCTACATATGGGCCATCTGATCGACAAAG
GCGGCTACCTGCGTTTTAAAGAACGGGTACTGCGCGGCGTTCAGCTCAATCCGGAAGCGATGGTCGCCGAACGCATTTAC
TGGGCCGAAGACGAATCCGTCGCGCGGATGCATCTCAAACCCGCCGGGCACTAA

Upstream 100 bases:

>100_bases
AAGTGCGGCCACTGCCGTATGGGCGAAGTGTATGTCTGCACCGACGGTCCGATATTTAACTACGCCGTCGCGCAACGTTT
TGCCGATTAAGGAGAACATC

Downstream 100 bases:

>100_bases
TCAACCACGCCATGGCCGCAGCCCGCCCACAGGCTGCGGCACGCTAAACCACATGACCAGCGCCGCGACAAGCAGTATGC
CCCCTCCAGCCAGCGCCAGC

Product: anaerobic sulfide reductase

Products: 3 NAD(P)+; NH4OH; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSIDIDIIKARAKNEYRLSKVRGEAMISVRIPGGILPAHLLTVARDIAETWGNGQIHLTTRQKLAMPGIRYEDIDNVNAA
LEPFLREIEIELCDVQVEDTKAGYLAIGGRNIVACQGNRICQKANTDTTGLSRRLEKLVYPSPYHLKTVIVGCPNDCAKA
SMADLGIIGVAKMRFTADRCIGCGACVKACSHHAVGCLALKNGKAVKEESACIGCGECVLACPTLAWQRKPDQLWQVRLG
GRTSKKTPRVGKLFLNWVTEDVIKQVIVNLYEFEKEMLGGKPIYLHMGHLIDKGGYLRFKERVLRGVQLNPEAMVAERIY
WAEDESVARMHLKPAGH

Sequences:

>Translated_337_residues
MSIDIDIIKARAKNEYRLSKVRGEAMISVRIPGGILPAHLLTVARDIAETWGNGQIHLTTRQKLAMPGIRYEDIDNVNAA
LEPFLREIEIELCDVQVEDTKAGYLAIGGRNIVACQGNRICQKANTDTTGLSRRLEKLVYPSPYHLKTVIVGCPNDCAKA
SMADLGIIGVAKMRFTADRCIGCGACVKACSHHAVGCLALKNGKAVKEESACIGCGECVLACPTLAWQRKPDQLWQVRLG
GRTSKKTPRVGKLFLNWVTEDVIKQVIVNLYEFEKEMLGGKPIYLHMGHLIDKGGYLRFKERVLRGVQLNPEAMVAERIY
WAEDESVARMHLKPAGH
>Mature_336_residues
SIDIDIIKARAKNEYRLSKVRGEAMISVRIPGGILPAHLLTVARDIAETWGNGQIHLTTRQKLAMPGIRYEDIDNVNAAL
EPFLREIEIELCDVQVEDTKAGYLAIGGRNIVACQGNRICQKANTDTTGLSRRLEKLVYPSPYHLKTVIVGCPNDCAKAS
MADLGIIGVAKMRFTADRCIGCGACVKACSHHAVGCLALKNGKAVKEESACIGCGECVLACPTLAWQRKPDQLWQVRLGG
RTSKKTPRVGKLFLNWVTEDVIKQVIVNLYEFEKEMLGGKPIYLHMGHLIDKGGYLRFKERVLRGVQLNPEAMVAERIYW
AEDESVARMHLKPAGH

Specific function: This enzyme catalyzes the hydrogen sulfide production from sulfite. It is strictly anaerobic. It is regulated by electron acceptors rather than by cysteine

COG id: COG2221

COG function: function code C; Dissimilatory sulfite reductase (desulfoviridin), alpha and beta subunits

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASRC_SALTI (P0A1Y3)

Other databases:

- EMBL:   AL627275
- EMBL:   AE014613
- RefSeq:   NP_457081.1
- RefSeq:   NP_804181.1
- ProteinModelPortal:   P0A1Y3
- GeneID:   1067914
- GeneID:   1249106
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t0306
- KEGG:   sty:STY2796
- HOGENOM:   HBG419834
- OMA:   GRTGKQT
- ProtClustDB:   CLSK2301998
- BioCyc:   SENT209261:T0306-MONOMER
- BioCyc:   SENT220341:STY2796-MONOMER
- GO:   GO:0005737
- GO:   GO:0006810
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR014261
- PRINTS:   PR00397
- TIGRFAMs:   TIGR02912

Pfam domain/function: PF00037 Fer4; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: 1.7.1.4 [C]

Molecular weight: Translated: 37291; Mature: 37160

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2; PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDIDIIKARAKNEYRLSKVRGEAMISVRIPGGILPAHLLTVARDIAETWGNGQIHLTT
CCCEEEEEEECCCCCHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
RQKLAMPGIRYEDIDNVNAALEPFLREIEIELCDVQVEDTKAGYLAIGGRNIVACQGNRI
HHHHCCCCCCCCCCCCHHHHHHHHHHHHEEEEEEEEEECCCCCEEEECCCEEEEECCCCH
CQKANTDTTGLSRRLEKLVYPSPYHLKTVIVGCPNDCAKASMADLGIIGVAKMRFTADRC
HHCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHCCEEEHHHHHCCHHHH
IGCGACVKACSHHAVGCLALKNGKAVKEESACIGCGECVLACPTLAWQRKPDQLWQVRLG
CCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCHHHCCCCCCEEEEEEC
GRTSKKTPRVGKLFLNWVTEDVIKQVIVNLYEFEKEMLGGKPIYLHMGHLIDKGGYLRFK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHCCCCCHHHH
ERVLRGVQLNPEAMVAERIYWAEDESVARMHLKPAGH
HHHHCCCCCCHHHHHHHHHEECCCCCEEEEEECCCCC
>Mature Secondary Structure 
SIDIDIIKARAKNEYRLSKVRGEAMISVRIPGGILPAHLLTVARDIAETWGNGQIHLTT
CCEEEEEEECCCCCHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
RQKLAMPGIRYEDIDNVNAALEPFLREIEIELCDVQVEDTKAGYLAIGGRNIVACQGNRI
HHHHCCCCCCCCCCCCHHHHHHHHHHHHEEEEEEEEEECCCCCEEEECCCEEEEECCCCH
CQKANTDTTGLSRRLEKLVYPSPYHLKTVIVGCPNDCAKASMADLGIIGVAKMRFTADRC
HHCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHCCEEEHHHHHCCHHHH
IGCGACVKACSHHAVGCLALKNGKAVKEESACIGCGECVLACPTLAWQRKPDQLWQVRLG
CCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCHHHCCCCCCEEEEEEC
GRTSKKTPRVGKLFLNWVTEDVIKQVIVNLYEFEKEMLGGKPIYLHMGHLIDKGGYLRFK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHCCCCCHHHH
ERVLRGVQLNPEAMVAERIYWAEDESVARMHLKPAGH
HHHHCCCCCCHHHHHHHHHEECCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: 3 NAD(P)H; nitrite [C]

Specific reaction: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504