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The map label for this gene is yfeJ

Identifier: 16765757

GI number: 16765757

Start: 2549810

End: 2550529

Strand: Direct

Name: yfeJ

Synonym: STM2437

Alternate gene names: 16765757

Gene position: 2549810-2550529 (Clockwise)

Preceding gene: 16765756

Following gene: 16765758

Centisome position: 52.49

GC content: 55.0

Gene sequence:

>720_bases
GTGCGAGTTCATTTTGTTGTCCATGAGTCGTTTGAATCCGCTGGCGCTTATCTGAAATGGGCTGAAGATCGCGGTTATAC
CATTTCATGGTCGCGCGTCTATGCCGGAGAAGCGCTACCGCCCAATGCCGATGAGTTCGATATGCTCGTGGTATTTGGCG
GTCCGCAGTCGCCGCGCACCACCCGCGAAGAGTGCCCGTACTTTGACTCTCGCGCCGAACAGCACTTAATTAATCAGGCA
GTTACGGCCCGGCGAATGGTTATCGGCATTTGTCTTGGTTCGCAGCTCATTGGCGAGGCGCTGGGCGCCGCTGTGTGCCA
AAGTCCGGAAAAAGAGATTGGTCACTACCCTATTACGCTCACTGAAGCCGGTCTGCGGCATCCGCTTATCGCCCACTTTG
GCTCCCCGTTAACCGTCGGCCACTGGCACAACGATATGCCAGGACTCACCGATCAGGCGACCGTGCTGGCCGAAAGCGAA
GGCTGTCCACGTCAGATAGTGCAGTACGGTAATTTTGTCTACGGCTTCCAGTGTCATATGGAATTTACCGTCGAGGCGGT
TGAAGGATTAATCCAACATTCGCAGCAGGAGCTGGCCGACGCGCAAGGGAAGCGTTTTATCCGCTCGGTCGCCGAGATGC
GCGCATGGAATTACCAGCAAATGAATGAAAAGCTGTGGCGTTTTCTGGATGAACTCACGCTGGCGCACTCACAGAAATAA

Upstream 100 bases:

>100_bases
ACGGTCTACGTATCACGCTGTCGACATTAAATGACAGCGAGATCAACACGCTGGCGGCTGATATCCATCAGGCGCTAAAT
CGCTAACAGGAGAAAATAAC

Downstream 100 bases:

>100_bases
CCACACCAGGAGTGCAACTTATTACCCGGCTAACGGTCTGAGGTGCAGTGATTATTGCATGAAAAAGGATTTTACCATGA
AGAAAATTGTTTGCGCCGTC

Product: glutamine amidotransferase

Products: AMP; L-Glutamate; GMP; H+; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRVHFVVHESFESAGAYLKWAEDRGYTISWSRVYAGEALPPNADEFDMLVVFGGPQSPRTTREECPYFDSRAEQHLINQA
VTARRMVIGICLGSQLIGEALGAAVCQSPEKEIGHYPITLTEAGLRHPLIAHFGSPLTVGHWHNDMPGLTDQATVLAESE
GCPRQIVQYGNFVYGFQCHMEFTVEAVEGLIQHSQQELADAQGKRFIRSVAEMRAWNYQQMNEKLWRFLDELTLAHSQK

Sequences:

>Translated_239_residues
MRVHFVVHESFESAGAYLKWAEDRGYTISWSRVYAGEALPPNADEFDMLVVFGGPQSPRTTREECPYFDSRAEQHLINQA
VTARRMVIGICLGSQLIGEALGAAVCQSPEKEIGHYPITLTEAGLRHPLIAHFGSPLTVGHWHNDMPGLTDQATVLAESE
GCPRQIVQYGNFVYGFQCHMEFTVEAVEGLIQHSQQELADAQGKRFIRSVAEMRAWNYQQMNEKLWRFLDELTLAHSQK
>Mature_239_residues
MRVHFVVHESFESAGAYLKWAEDRGYTISWSRVYAGEALPPNADEFDMLVVFGGPQSPRTTREECPYFDSRAEQHLINQA
VTARRMVIGICLGSQLIGEALGAAVCQSPEKEIGHYPITLTEAGLRHPLIAHFGSPLTVGHWHNDMPGLTDQATVLAESE
GCPRQIVQYGNFVYGFQCHMEFTVEAVEGLIQHSQQELADAQGKRFIRSVAEMRAWNYQQMNEKLWRFLDELTLAHSQK

Specific function: Catalyzes The Synthesis Of Gmp From Xmp. [C]

COG id: COG0518

COG function: function code F; GMP synthase - Glutamine amidotransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFEJ_SALTY (P40194)

Other databases:

- EMBL:   U11243
- EMBL:   AE006468
- RefSeq:   NP_461372.1
- ProteinModelPortal:   P40194
- PRIDE:   P40194
- GeneID:   1253959
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2437
- NMPDR:   fig|99287.1.peg.2353
- HOGENOM:   HBG711623
- OMA:   TEIGWYP
- ProtClustDB:   PRK08250
- BioCyc:   STYP99287:STM2437-MONOMER
- InterPro:   IPR017926
- InterPro:   IPR000991

Pfam domain/function: PF00117 GATase

EC number: 6.3.5.2 [C]

Molecular weight: Translated: 26952; Mature: 26952

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVHFVVHESFESAGAYLKWAEDRGYTISWSRVYAGEALPPNADEFDMLVVFGGPQSPRT
CEEEEEEECCHHHCCCEEEEECCCCEEEEEHHEEECCCCCCCCCCEEEEEEECCCCCCCC
TREECPYFDSRAEQHLINQAVTARRMVIGICLGSQLIGEALGAAVCQSPEKEIGHYPITL
CHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEE
TEAGLRHPLIAHFGSPLTVGHWHNDMPGLTDQATVLAESEGCPRQIVQYGNFVYGFQCHM
ECCCCCCHHHHHCCCCCEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCEEEEEEEEH
EFTVEAVEGLIQHSQQELADAQGKRFIRSVAEMRAWNYQQMNEKLWRFLDELTLAHSQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVHFVVHESFESAGAYLKWAEDRGYTISWSRVYAGEALPPNADEFDMLVVFGGPQSPRT
CEEEEEEECCHHHCCCEEEEECCCCEEEEEHHEEECCCCCCCCCCEEEEEEECCCCCCCC
TREECPYFDSRAEQHLINQAVTARRMVIGICLGSQLIGEALGAAVCQSPEKEIGHYPITL
CHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEE
TEAGLRHPLIAHFGSPLTVGHWHNDMPGLTDQATVLAESEGCPRQIVQYGNFVYGFQCHM
ECCCCCCHHHHHCCCCCEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCEEEEEEEEH
EFTVEAVEGLIQHSQQELADAQGKRFIRSVAEMRAWNYQQMNEKLWRFLDELTLAHSQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 2 {1-ribosyl-4,6-dihydroxypyrazolo[3,4-d]-pyrimidine} 0.058 {XMP}} 0.029 {XMP}} 1 {NH4+}} 0.42 {6-thioXMP}} 0.53 {ATP}} 0.35 {8-azaXMP}} [C]

Substrates: ATP; L-Glutamine; H2O; Xanthosine 5'-phosphate [C]

Specific reaction: ATP + L-Glutamine + H2O + Xanthosine 5'-phosphate --> AMP + L-Glutamate + GMP + (2) H+ + Diphosphate [C]

General reaction: Amination [C]

Inhibitor: 1, N6-Ethenoadenosine; 1-Methyl adenosine 5'-phosphate 5'-phosphate; 2', 3'-Dihydroadenosine; 2'-Deoxyadenosine; 2, 6-Diaminopurine ribonucleoside; 2-Amino-6-methyl thiopurine ribonucleoside; 2-Fluoroadenosine; 3'-Deoxyadenosine; 5'-Azido-5'-deoxyadenosin

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7612925; 11677609