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The map label for this gene is ptsJ

Identifier: 16765756

GI number: 16765756

Start: 2548503

End: 2549795

Strand: Direct

Name: ptsJ

Synonym: STM2436

Alternate gene names: 16765756

Gene position: 2548503-2549795 (Clockwise)

Preceding gene: 16765753

Following gene: 16765757

Centisome position: 52.47

GC content: 58.93

Gene sequence:

>1293_bases
ATGATCGACGGAAAAACCGCTAACGAAATTTTTGACAGTATCCGTCAACACATTATCGCCGGCACGTTACGGGCGGAGGA
TTCGCTCCCGCCGGTACGGGAGCTGGCAAGCGAATTAAAGGTGAACCGCAATACCGTCGCAGCGGCGTATAAACGGCTGA
TAACGGCGGGACTGGCGCAAAGTTTAGGCCGCAATGGGACGGTGATTAAAGGCAGTCCGTCTCCCGTAGCGCTGGAAGGC
GGCGATCCACATACGCCTCTGCACGACCTTTCCGGCGGCAATCCCGATCCGCAGCGTCTGCCTGACCTTAGCCGTTATTT
TGCCCGTCTCAGCCGCACGCCGCATCTCTACGGCGATGCGCCCGTTTCCCCTGAACTACACGCCTGGGCGGCGCGCTGGC
TACGGGATGCCACCCCCGTCGCAGGCGAAATTGATATCACCAGCGGAGCTATCGACGCCATTGAAAGACTGCTTTGCGCG
CATCTGCTGCCGGGCGACAGCGTCGCGGTGGAAGATCCCTGTTTTTTAAGCAGTATCAATATGCTGCGCTATGCCGGTTT
TAGCGCCAGCCCGGTCAGTGTGGATAGCGAAGGAATGCAGCCTGAAAAGCTGGAACGGGCGCTAAATCAGGGCGCGCGGG
CAGTGATCTTAACGCCGCGCGCGCACAACCCTACCGGCTGTAGCCTGAGCGCTCGCCGTGCCGCTGCGCTGCAAAATATG
CTGGCGCGTTACCCTCAGGTAGTGGTCATTATCGACGATCACTTCGCCCTGCTTTCTTCGTCGCCCTGGCAGCCGGTTAT
TGCGCAGACAACGCAACACTGGGCGGTGATACGCTCCGTTTCCAAAACCCTCGGCCCCGATTTACGTCTTGCTATTGTCG
CCAGCGACAGCGCGACCTCGGCAAAACTTCGGCTGCGGCTAAATGCCGGTAGTCAGTGGGTCAGCCATCTGTTACAGGAT
CTGGTCTACGCCTGCCTGACCGACCCTGAGTATCAGCACAGGCTGACGCAAACGCGGCTGTTTTACGCCGCCCGGCAACA
AAAACTGGCCCGCGCCCTACAGCAATATGGCATCGCCATTTCTCCCGGCGACGGCGTGAATGCCTGGCTCCCACTGGACA
CCCATAGCCAGGCTACCGCGTTTACGTTGGCAAAATCCGGCTGGCTGGTACGTGAGGGAGAAGCCTTTGGCGTAAGCGCG
CCGTCTCACGGTCTACGTATCACGCTGTCGACATTAAATGACAGCGAGATCAACACGCTGGCGGCTGATATCCATCAGGC
GCTAAATCGCTAA

Upstream 100 bases:

>100_bases
ATCACTCTCTTGTCCCATAAAATTTTCCTCTCGCTGGACAGCAGGCTACCGCCTCATTATCATTGTCATATGCTAAAATT
CATTTTGTCATAGGTCAATA

Downstream 100 bases:

>100_bases
CAGGAGAAAATAACGTGCGAGTTCATTTTGTTGTCCATGAGTCGTTTGAATCCGCTGGCGCTTATCTGAAATGGGCTGAA
GATCGCGGTTATACCATTTC

Product: regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MIDGKTANEIFDSIRQHIIAGTLRAEDSLPPVRELASELKVNRNTVAAAYKRLITAGLAQSLGRNGTVIKGSPSPVALEG
GDPHTPLHDLSGGNPDPQRLPDLSRYFARLSRTPHLYGDAPVSPELHAWAARWLRDATPVAGEIDITSGAIDAIERLLCA
HLLPGDSVAVEDPCFLSSINMLRYAGFSASPVSVDSEGMQPEKLERALNQGARAVILTPRAHNPTGCSLSARRAAALQNM
LARYPQVVVIIDDHFALLSSSPWQPVIAQTTQHWAVIRSVSKTLGPDLRLAIVASDSATSAKLRLRLNAGSQWVSHLLQD
LVYACLTDPEYQHRLTQTRLFYAARQQKLARALQQYGIAISPGDGVNAWLPLDTHSQATAFTLAKSGWLVREGEAFGVSA
PSHGLRITLSTLNDSEINTLAADIHQALNR

Sequences:

>Translated_430_residues
MIDGKTANEIFDSIRQHIIAGTLRAEDSLPPVRELASELKVNRNTVAAAYKRLITAGLAQSLGRNGTVIKGSPSPVALEG
GDPHTPLHDLSGGNPDPQRLPDLSRYFARLSRTPHLYGDAPVSPELHAWAARWLRDATPVAGEIDITSGAIDAIERLLCA
HLLPGDSVAVEDPCFLSSINMLRYAGFSASPVSVDSEGMQPEKLERALNQGARAVILTPRAHNPTGCSLSARRAAALQNM
LARYPQVVVIIDDHFALLSSSPWQPVIAQTTQHWAVIRSVSKTLGPDLRLAIVASDSATSAKLRLRLNAGSQWVSHLLQD
LVYACLTDPEYQHRLTQTRLFYAARQQKLARALQQYGIAISPGDGVNAWLPLDTHSQATAFTLAKSGWLVREGEAFGVSA
PSHGLRITLSTLNDSEINTLAADIHQALNR
>Mature_430_residues
MIDGKTANEIFDSIRQHIIAGTLRAEDSLPPVRELASELKVNRNTVAAAYKRLITAGLAQSLGRNGTVIKGSPSPVALEG
GDPHTPLHDLSGGNPDPQRLPDLSRYFARLSRTPHLYGDAPVSPELHAWAARWLRDATPVAGEIDITSGAIDAIERLLCA
HLLPGDSVAVEDPCFLSSINMLRYAGFSASPVSVDSEGMQPEKLERALNQGARAVILTPRAHNPTGCSLSARRAAALQNM
LARYPQVVVIIDDHFALLSSSPWQPVIAQTTQHWAVIRSVSKTLGPDLRLAIVASDSATSAKLRLRLNAGSQWVSHLLQD
LVYACLTDPEYQHRLTQTRLFYAARQQKLARALQQYGIAISPGDGVNAWLPLDTHSQATAFTLAKSGWLVREGEAFGVSA
PSHGLRITLSTLNDSEINTLAADIHQALNR

Specific function: Putative transcriptional regulator of the ptsHI/crr operon

COG id: COG1167

COG function: function code KE; Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787710, Length=418, Percent_Identity=28.4688995215311, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790797, Length=319, Percent_Identity=30.4075235109718, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): PTSJ_SALTY (P40193)

Other databases:

- EMBL:   U11243
- EMBL:   AE006468
- RefSeq:   NP_461371.1
- ProteinModelPortal:   P40193
- SMR:   P40193
- PRIDE:   P40193
- GeneID:   1253958
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2436
- NMPDR:   fig|99287.1.peg.2352
- HOGENOM:   HBG416807
- OMA:   GNPDPAR
- ProtClustDB:   PRK15481
- BioCyc:   STYP99287:STM2436-MONOMER
- GO:   GO:0005622
- InterPro:   IPR004839
- InterPro:   IPR000524
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR011991
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00345

Pfam domain/function: PF00155 Aminotran_1_2; PF00392 GntR; SSF53383 PyrdxlP-dep_Trfase_major

EC number: NA

Molecular weight: Translated: 46508; Mature: 46508

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDGKTANEIFDSIRQHIIAGTLRAEDSLPPVRELASELKVNRNTVAAAYKRLITAGLAQ
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SLGRNGTVIKGSPSPVALEGGDPHTPLHDLSGGNPDPQRLPDLSRYFARLSRTPHLYGDA
HHCCCCCEEECCCCCEEECCCCCCCCCHHCCCCCCCHHHCHHHHHHHHHHCCCCCCCCCC
PVSPELHAWAARWLRDATPVAGEIDITSGAIDAIERLLCAHLLPGDSVAVEDPCFLSSIN
CCCCHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHH
MLRYAGFSASPVSVDSEGMQPEKLERALNQGARAVILTPRAHNPTGCSLSARRAAALQNM
HHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
LARYPQVVVIIDDHFALLSSSPWQPVIAQTTQHWAVIRSVSKTLGPDLRLAIVASDSATS
HHHCCEEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
AKLRLRLNAGSQWVSHLLQDLVYACLTDPEYQHRLTQTRLFYAARQQKLARALQQYGIAI
EEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
SPGDGVNAWLPLDTHSQATAFTLAKSGWLVREGEAFGVSAPSHGLRITLSTLNDSEINTL
CCCCCCCEEEECCCCCCHHEEEEECCCCEEECCCEECCCCCCCCEEEEEEECCCCHHHHH
AADIHQALNR
HHHHHHHHCC
>Mature Secondary Structure
MIDGKTANEIFDSIRQHIIAGTLRAEDSLPPVRELASELKVNRNTVAAAYKRLITAGLAQ
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SLGRNGTVIKGSPSPVALEGGDPHTPLHDLSGGNPDPQRLPDLSRYFARLSRTPHLYGDA
HHCCCCCEEECCCCCEEECCCCCCCCCHHCCCCCCCHHHCHHHHHHHHHHCCCCCCCCCC
PVSPELHAWAARWLRDATPVAGEIDITSGAIDAIERLLCAHLLPGDSVAVEDPCFLSSIN
CCCCHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHH
MLRYAGFSASPVSVDSEGMQPEKLERALNQGARAVILTPRAHNPTGCSLSARRAAALQNM
HHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHH
LARYPQVVVIIDDHFALLSSSPWQPVIAQTTQHWAVIRSVSKTLGPDLRLAIVASDSATS
HHHCCEEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
AKLRLRLNAGSQWVSHLLQDLVYACLTDPEYQHRLTQTRLFYAARQQKLARALQQYGIAI
EEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
SPGDGVNAWLPLDTHSQATAFTLAKSGWLVREGEAFGVSAPSHGLRITLSTLNDSEINTL
CCCCCCCEEEECCCCCCHHEEEEECCCCEEECCCEECCCCCCCCEEEEEEECCCCHHHHH
AADIHQALNR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7612925; 11677609