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The map label for this gene is alkB

Identifier: 16765592

GI number: 16765592

Start: 2362481

End: 2363131

Strand: Reverse

Name: alkB

Synonym: STM2264

Alternate gene names: 16765592

Gene position: 2363131-2362481 (Counterclockwise)

Preceding gene: 16765593

Following gene: 16765591

Centisome position: 48.65

GC content: 58.83

Gene sequence:

>651_bases
ATGCTGGATCTATTTGCTGATGAAGCGCCCTGGCAAGAGCCCCTGGCGCCTGGCGCGGTGGTGTTGCGCCGCTTTGCGTT
TCGCGCGGCGCAGTCGCTATTAGACGATATCGGGTTTGTCGCCAGTCAGTCTCCGTTTCGTCAGATGGTGACGCCGGGCG
GCTACACCATGTCGGTGGCGATGACCAACTGCGGCGCGTTAGGGTGGACTACCGATCGTCACGGTTATTGTTATGCCGTG
CGCGATCCGTTGACTGATAAACCCTGGCCTGCGCTGCCGTTATCGTTTGCCAGCGTATGTCGTCAGGCGGCAATCGCGGC
AGGGTATGCGAGCTTCCAACCGGACGCCTGCCTGATCAATCGCTACGCGCCCGGCGCAAAACTGTCGCTGCATCAGGATA
AAGACGAGCCGGATCTGCGCGCGCCTATTGTCTCAGTTTCGTTGGGGGTGCCGGCGGTTTTTCAGTTTGGCGGCCTGCGT
CGCAGCGACCCGATCCAGCGGATCTTACTGGAGCATGGCGATATCGTGGTCTGGGGCGGCGAGTCCCGGCTGTTTTATCA
TGGTATCCAGCCGCTTAAAGCGGGCTTTCATCCCATGACCGGTGAATTTCGTTACAATCTCACCTTCCGTCAGGCAGCTG
AAAAAGAATAA

Upstream 100 bases:

>100_bases
ATCGGGTCGTGCGTCGCGATGGCGCGCTCTCCGGTTATCGTTGGGGCGTGCGTCGAAAAGCGCAGCTATTAAAGCGAGAA
GCGCAAAAAGAGGAGTAGCA

Downstream 100 bases:

>100_bases
AAATAAGAATTATTATTGCTGTGCAGGTGGAGTTAGTTAAACTGCGGGCTGTTATTATCGGTCCGGGTTGTATGCATGGA
ACTTCTTATTCTTGTCTGGC

Product: DNA repair system protein

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MLDLFADEAPWQEPLAPGAVVLRRFAFRAAQSLLDDIGFVASQSPFRQMVTPGGYTMSVAMTNCGALGWTTDRHGYCYAV
RDPLTDKPWPALPLSFASVCRQAAIAAGYASFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGVPAVFQFGGLR
RSDPIQRILLEHGDIVVWGGESRLFYHGIQPLKAGFHPMTGEFRYNLTFRQAAEKE

Sequences:

>Translated_216_residues
MLDLFADEAPWQEPLAPGAVVLRRFAFRAAQSLLDDIGFVASQSPFRQMVTPGGYTMSVAMTNCGALGWTTDRHGYCYAV
RDPLTDKPWPALPLSFASVCRQAAIAAGYASFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGVPAVFQFGGLR
RSDPIQRILLEHGDIVVWGGESRLFYHGIQPLKAGFHPMTGEFRYNLTFRQAAEKE
>Mature_216_residues
MLDLFADEAPWQEPLAPGAVVLRRFAFRAAQSLLDDIGFVASQSPFRQMVTPGGYTMSVAMTNCGALGWTTDRHGYCYAV
RDPLTDKPWPALPLSFASVCRQAAIAAGYASFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGVPAVFQFGGLR
RSDPIQRILLEHGDIVVWGGESRLFYHGIQPLKAGFHPMTGEFRYNLTFRQAAEKE

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain

Homologues:

Organism=Homo sapiens, GI87298840, Length=123, Percent_Identity=30.8943089430894, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1788541, Length=216, Percent_Identity=79.6296296296296, Blast_Score=366, Evalue=1e-103,
Organism=Drosophila melanogaster, GI45555401, Length=127, Percent_Identity=37.007874015748, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALKB_SALTY (P37462)

Other databases:

- EMBL:   AE006468
- EMBL:   D90221
- PIR:   B39433
- RefSeq:   NP_461207.1
- ProteinModelPortal:   P37462
- SMR:   P37462
- GeneID:   1253786
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2264
- HOGENOM:   HBG595262
- OMA:   GFAPDAC
- ProtClustDB:   PRK15401
- BioCyc:   STYP99287:STM2264-MONOMER

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23787; Mature: 23787

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS51471 FE2OG_OXY

Important sites: BINDING 69-69 BINDING 135-135 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLFADEAPWQEPLAPGAVVLRRFAFRAAQSLLDDIGFVASQSPFRQMVTPGGYTMSVA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEE
MTNCGALGWTTDRHGYCYAVRDPLTDKPWPALPLSFASVCRQAAIAAGYASFQPDACLIN
ECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
RYAPGAKLSLHQDKDEPDLRAPIVSVSLGVPAVFQFGGLRRSDPIQRILLEHGDIVVWGG
CCCCCCEEEECCCCCCCCCCCCEEEEECCCCHHHHHCCCCCCCHHHHHHHHCCCEEEECC
ESRLFYHGIQPLKAGFHPMTGEFRYNLTFRQAAEKE
CCEEEEECCCHHHCCCCCCCCCEEEEEEHHHHCCCC
>Mature Secondary Structure
MLDLFADEAPWQEPLAPGAVVLRRFAFRAAQSLLDDIGFVASQSPFRQMVTPGGYTMSVA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEE
MTNCGALGWTTDRHGYCYAVRDPLTDKPWPALPLSFASVCRQAAIAAGYASFQPDACLIN
ECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
RYAPGAKLSLHQDKDEPDLRAPIVSVSLGVPAVFQFGGLRRSDPIQRILLEHGDIVVWGG
CCCCCCEEEECCCCCCCCCCCCEEEEECCCCHHHHHCCCCCCCHHHHHHHHCCCEEEECC
ESRLFYHGIQPLKAGFHPMTGEFRYNLTFRQAAEKE
CCEEEEECCCHHHCCCCCCCCCEEEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 1904855