Click here to switch to the map view.

The map label for this gene is ada

Identifier: 16765593

GI number: 16765593

Start: 2363134

End: 2364195

Strand: Reverse

Name: ada

Synonym: STM2265

Alternate gene names: 16765593

Gene position: 2364195-2363134 (Counterclockwise)

Preceding gene: 16765594

Following gene: 16765592

Centisome position: 48.67

GC content: 60.73

Gene sequence:

>1062_bases
ATGATGAAAAAAGCGTTACTTATCGATGATGAATGCTGGCTGCGGGTGCAGGCGCGCGATGCCAGCGCGGATGGGCGTTT
CGTTTTTGCGGTGCGAACCACCGGCGTTTTTTGCCGTCCTTCTTGTCGCTCGAAGCGGGCGTTACGTAAAAATGTTCGCT
TTTTTGCCAACGCGCAGCAGGCGCTGGACGCCGGTTTTCGCCCCTGCAAGCGCTGTCAGCCGGATAATGCGCGCGCGCAG
CAACGGCGGTTGGATAAGATTGCCTGCGCCTGCCGTTTGCTTGAGCAGGAGACGCCGGTAACGCTGGCGTTTCTGGCGCA
GGCGGTGGCGATGAGCCCGTTTCATCTGCACCGTCTGTTTAAAGCGAGCACCGGAATGACGCCGAAAGGGTGGCAGCAGG
CGTGGCGCGCCCGGCGGCTGCGTGAGGCTTTGGCGAAAGGAGAGCCGATCACGGCGGCTATTTACCGCGCTGGCTTCCCG
GATAGCAGTAGCTACTACCGTCATGCCGACCAGACGCTGGGCATGACGGCAAAACAGTTTCGCAAAGGCGGCGATAATGT
CTCCGTTCGCTATGCGCTGACGGACTGGGTTTACGGACGGTGCCTGGTGGCGGAGAGCGAGCGGGGGATTTGCGCGATTC
TCCCCGGTGATAGCGACGACGCGCTACTGGCTGAATTACACACCCTTTTCCCGTCGGCCCGCCACGAACCTGCTGACGCG
CTTTTTCAGCAACGGGTGCGGCAGGTTGTCGCGGCTATCAACACACGCGATGTGCTGCTCTCGTTGCCGCTGGATATCCA
GGGAACCGCGTTTCAACAGCAGGTCTGGCAGGCGTTATGCGCAATTCCCTGCGGCGAAACCGTAAGCTATCAACAGCTTG
CCGCGACTATCGGCAAACCCACGGCGGTACGCGCGGTCGCCAGCGCGTGCGGCGCGAATAAACTGGCGATGGTGATCCCG
TGTCATCGGGTCGTGCGTCGCGATGGCGCGCTCTCCGGTTATCGTTGGGGCGTGCGTCGAAAAGCGCAGCTATTAAAGCG
AGAAGCGCAAAAAGAGGAGTAG

Upstream 100 bases:

>100_bases
TGGTGAGCGATAAGAATTAAAACGCAAGATTACGGGTTTTTGTGGGCGGATTTACGGACACACTGCATGTAAGCAAGCAC
TATGCTTAATGAAGGAGTCT

Downstream 100 bases:

>100_bases
CAATGCTGGATCTATTTGCTGATGAAGCGCCCTGGCAAGAGCCCCTGGCGCCTGGCGCGGTGGTGTTGCGCCGCTTTGCG
TTTCGCGCGGCGCAGTCGCT

Product: O6-methylguanine-DNA methyltransferase/transcription activator/repressor

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MMKKALLIDDECWLRVQARDASADGRFVFAVRTTGVFCRPSCRSKRALRKNVRFFANAQQALDAGFRPCKRCQPDNARAQ
QRRLDKIACACRLLEQETPVTLAFLAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAKGEPITAAIYRAGFP
DSSSYYRHADQTLGMTAKQFRKGGDNVSVRYALTDWVYGRCLVAESERGICAILPGDSDDALLAELHTLFPSARHEPADA
LFQQRVRQVVAAINTRDVLLSLPLDIQGTAFQQQVWQALCAIPCGETVSYQQLAATIGKPTAVRAVASACGANKLAMVIP
CHRVVRRDGALSGYRWGVRRKAQLLKREAQKEE

Sequences:

>Translated_353_residues
MMKKALLIDDECWLRVQARDASADGRFVFAVRTTGVFCRPSCRSKRALRKNVRFFANAQQALDAGFRPCKRCQPDNARAQ
QRRLDKIACACRLLEQETPVTLAFLAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAKGEPITAAIYRAGFP
DSSSYYRHADQTLGMTAKQFRKGGDNVSVRYALTDWVYGRCLVAESERGICAILPGDSDDALLAELHTLFPSARHEPADA
LFQQRVRQVVAAINTRDVLLSLPLDIQGTAFQQQVWQALCAIPCGETVSYQQLAATIGKPTAVRAVASACGANKLAMVIP
CHRVVRRDGALSGYRWGVRRKAQLLKREAQKEE
>Mature_353_residues
MMKKALLIDDECWLRVQARDASADGRFVFAVRTTGVFCRPSCRSKRALRKNVRFFANAQQALDAGFRPCKRCQPDNARAQ
QRRLDKIACACRLLEQETPVTLAFLAQAVAMSPFHLHRLFKASTGMTPKGWQQAWRARRLREALAKGEPITAAIYRAGFP
DSSSYYRHADQTLGMTAKQFRKGGDNVSVRYALTDWVYGRCLVAESERGICAILPGDSDDALLAELHTLFPSARHEPADA
LFQQRVRQVVAAINTRDVLLSLPLDIQGTAFQQQVWQALCAIPCGETVSYQQLAATIGKPTAVRAVASACGANKLAMVIP
CHRVVRRDGALSGYRWGVRRKAQLLKREAQKEE

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain

Homologues:

Organism=Homo sapiens, GI197304670, Length=92, Percent_Identity=41.304347826087, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788542, Length=353, Percent_Identity=74.2209631728045, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1787596, Length=93, Percent_Identity=46.2365591397849, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=163, Percent_Identity=40.4907975460123, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI115533070, Length=128, Percent_Identity=44.53125, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320001, Length=91, Percent_Identity=43.956043956044, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137554, Length=178, Percent_Identity=37.6404494382022, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ADA_SALTY (P26189)

Other databases:

- EMBL:   D90221
- EMBL:   AE006468
- PIR:   A39433
- RefSeq:   NP_461208.1
- ProteinModelPortal:   P26189
- GeneID:   1253787
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2265
- NMPDR:   fig|99287.1.peg.2189
- HOGENOM:   HBG752398
- OMA:   SNSRFYE
- ProtClustDB:   PRK15435
- BioCyc:   STYP99287:STM2265-MONOMER
- BRENDA:   2.1.1.63
- GO:   GO:0005622
- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991
- Gene3D:   G3DSA:3.40.10.10
- Gene3D:   G3DSA:1.10.10.60
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR10815
- PIRSF:   PIRSF000409
- SMART:   SM00342
- TIGRFAMs:   TIGR00589

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N; SSF57884 Ada_DNA_repair_Zn-bd; SSF46689 Homeodomain_like; SSF46767 MethylDNA_cys_mtrans_DNA_bd

EC number: =2.1.1.63

Molecular weight: Translated: 39349; Mature: 39349

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS00041 HTH_ARAC_FAMILY_1; PS01124 HTH_ARAC_FAMILY_2; PS00374 MGMT

Important sites: ACT_SITE 38-38 ACT_SITE 321-321 BINDING 34-34 BINDING 43-43 BINDING 67-67

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKALLIDDECWLRVQARDASADGRFVFAVRTTGVFCRPSCRSKRALRKNVRFFANAQQ
CCCCCEEECCCCEEEEEECCCCCCCCEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHH
ALDAGFRPCKRCQPDNARAQQRRLDKIACACRLLEQETPVTLAFLAQAVAMSPFHLHRLF
HHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHH
KASTGMTPKGWQQAWRARRLREALAKGEPITAAIYRAGFPDSSSYYRHADQTLGMTAKQF
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHH
RKGGDNVSVRYALTDWVYGRCLVAESERGICAILPGDSDDALLAELHTLFPSARHEPADA
HCCCCCEEEEEEEHHHHHCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHH
LFQQRVRQVVAAINTRDVLLSLPLDIQGTAFQQQVWQALCAIPCGETVSYQQLAATIGKP
HHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
TAVRAVASACGANKLAMVIPCHRVVRRDGALSGYRWGVRRKAQLLKREAQKEE
HHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMKKALLIDDECWLRVQARDASADGRFVFAVRTTGVFCRPSCRSKRALRKNVRFFANAQQ
CCCCCEEECCCCEEEEEECCCCCCCCEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHH
ALDAGFRPCKRCQPDNARAQQRRLDKIACACRLLEQETPVTLAFLAQAVAMSPFHLHRLF
HHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHH
KASTGMTPKGWQQAWRARRLREALAKGEPITAAIYRAGFPDSSSYYRHADQTLGMTAKQF
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHH
RKGGDNVSVRYALTDWVYGRCLVAESERGICAILPGDSDDALLAELHTLFPSARHEPADA
HCCCCCEEEEEEEHHHHHCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHH
LFQQRVRQVVAAINTRDVLLSLPLDIQGTAFQQQVWQALCAIPCGETVSYQQLAATIGKP
HHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
TAVRAVASACGANKLAMVIPCHRVVRRDGALSGYRWGVRRKAQLLKREAQKEE
HHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904855; 11677609