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The map label for this gene is wcaK [H]

Identifier: 16765431

GI number: 16765431

Start: 2182659

End: 2183939

Strand: Reverse

Name: wcaK [H]

Synonym: STM2101

Alternate gene names: 16765431

Gene position: 2183939-2182659 (Counterclockwise)

Preceding gene: 16765432

Following gene: 16765430

Centisome position: 44.96

GC content: 53.24

Gene sequence:

>1281_bases
ATGAAATTATTAATTTTAGGCAACCACACATGCGGCAACCGTGGTGATAGCGCCATTATGCGCGGTTTGCTTGATGCCAT
CCGTCAACAGGCGCCAGAGGCGGAGATGGATGTGATGAGCCGTTTCCCGGTGAGTTCCGCCTGGTTGCAGGGGCGCCCGA
TTATTGCCGACCCGCTGTATCAGTTAAGCCAGAAACAGCAGGCGGCGGCAGGGCTTAACGGTCGGGTGAAAAAAGTATTA
CGCCGTCGTTTTCAGCATAAAATTTTATTGTCTAAAGTCGCCCAGGAAGGATCGCTGCGTAATTTTGCTATCGCGCCGGA
ATTTGCCGAATTCGCGCAATTTATTGCGCAGTATGACGCCGTCATTCAGGTGGGCGGTTCAAACTATGTCGACTTATACG
GTTTGACTCACTTTGAATATCCGCTGTGCGCGTTTATGGCGAACAAACCAATTTATATGGTGGGACATAGCGTCGGCCCT
TTCCAGAATCCTGACTTTAACCAGCTCGCCAATTATGTTTTTGGGCGCACCAGTGCGCTGATCCTGCGTGAAACCGTCAG
CCGCGATCTGATGACCGCCGCGCAGATCGATACCCGGAAAGTGGAGCAGGGGGTGGATACCGCCTGGTTAGTCGAACGCC
GTCACGACGAGTTTGTTGCCAGTTATGCTGTGCAGCACTGGCTGGACGTCACCGCGCGCCGCAAAACCGTCGCGATTACG
TTGCGCGATCTGGAGCCGTTTGATAAACGCCTGGGCACCACTCAGCAGGCCTATGAGCAGGCATTTGCCCAGGTCGTGAA
TCGCCTGATTGCGGACGGTTATCAGGTACTGGCGCTGTCAACCTGCACCAGTATCGATCGCTATAACCGCGATGACCGGA
TGGTTGCGTTACGGATGGCGAAGTATGTAAACGACTCAGAGAATTTCCATATCGCGATGGACGAACTCAATGACGTGGAA
ATCGGAAAGATCCTCGCCTCCTGCGTACTGACCATCGGGACGCGCCTGCATTCCGCCATTATCTCCATGAATTTTGGCAC
ACCCGCCATCGCCATTAATTATGAACATAAATCCGCCGGGATTATGCAGCAGCTAGGCCTGGCGGAATTGGCCATCGATA
TCCGTCACTTGCTGAACGGTTCATTACAAAGCGTGGTGGCCGATACGCTCAATCAATTGCCTGCGCTAAAGGCAAAAGTG
GCTAACGCCGTCACCGAGGAGCGGGCGAAAGGGTTCACGATGATCAAATCTGTTCTCGACAGAATTCGGGAGGCGCAATG
A

Upstream 100 bases:

>100_bases
ACGCAATGCGCTGGTGATGGAAATTAAAAATCAGCTTGTTGGCAGTGCAAAAATGAAAAAGTTATTACGTGTCGGATGAC
GGGGATATAAGAGGCTATTT

Downstream 100 bases:

>100_bases
AAGTCAGCTTTTTTCTGCTGAAATTTCCACTCTCATCGGAAACCTTTGTGCTGAATCAGATTACTGCGTTTATTGATATG
GGCCATGAGGTGGAGATTGT

Product: pyruvyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLYQLSQKQQAAAGLNGRVKKVL
RRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDAVIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGP
FQNPDFNQLANYVFGRTSALILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT
LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMAKYVNDSENFHIAMDELNDVE
IGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKV
ANAVTEERAKGFTMIKSVLDRIREAQ

Sequences:

>Translated_426_residues
MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLYQLSQKQQAAAGLNGRVKKVL
RRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDAVIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGP
FQNPDFNQLANYVFGRTSALILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT
LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMAKYVNDSENFHIAMDELNDVE
IGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKV
ANAVTEERAKGFTMIKSVLDRIREAQ
>Mature_426_residues
MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLYQLSQKQQAAAGLNGRVKKVL
RRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDAVIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGP
FQNPDFNQLANYVFGRTSALILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT
LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMAKYVNDSENFHIAMDELNDVE
IGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAGIMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKV
ANAVTEERAKGFTMIKSVLDRIREAQ

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2327

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide pyruvyl transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788358, Length=426, Percent_Identity=67.8403755868545, Blast_Score=623, Evalue=1e-180,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007345 [H]

Pfam domain/function: PF04230 PS_pyruv_trans [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 47589; Mature: 47589

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLY
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCCCCCHHHHHH
QLSQKQQAAAGLNGRVKKVLRRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHH
VIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGPFQNPDFNQLANYVFGRTSAL
HEEECCCCEEEEECCCHHCCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHH
ILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMA
EECCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHCCCCHHHHHHHHH
KYVNDSENFHIAMDELNDVEIGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAG
HHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCHHHH
IMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKVANAVTEERAKGFTMIKSVLD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIREAQ
HHHCCC
>Mature Secondary Structure
MKLLILGNHTCGNRGDSAIMRGLLDAIRQQAPEAEMDVMSRFPVSSAWLQGRPIIADPLY
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCCCCCHHHHHH
QLSQKQQAAAGLNGRVKKVLRRRFQHKILLSKVAQEGSLRNFAIAPEFAEFAQFIAQYDA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHH
VIQVGGSNYVDLYGLTHFEYPLCAFMANKPIYMVGHSVGPFQNPDFNQLANYVFGRTSAL
HEEECCCCEEEEECCCHHCCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHH
ILRETVSRDLMTAAQIDTRKVEQGVDTAWLVERRHDEFVASYAVQHWLDVTARRKTVAIT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
LRDLEPFDKRLGTTQQAYEQAFAQVVNRLIADGYQVLALSTCTSIDRYNRDDRMVALRMA
EECCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHCCCCHHHHHHHHH
KYVNDSENFHIAMDELNDVEIGKILASCVLTIGTRLHSAIISMNFGTPAIAINYEHKSAG
HHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCHHHH
IMQQLGLAELAIDIRHLLNGSLQSVVADTLNQLPALKAKVANAVTEERAKGFTMIKSVLD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIREAQ
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9278503 [H]