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The map label for this gene is wcaL

Identifier: 16765430

GI number: 16765430

Start: 2181442

End: 2182662

Strand: Reverse

Name: wcaL

Synonym: STM2100

Alternate gene names: 16765430

Gene position: 2182662-2181442 (Counterclockwise)

Preceding gene: 16765431

Following gene: 16765429

Centisome position: 44.93

GC content: 56.18

Gene sequence:

>1221_bases
ATGAAAGTCAGCTTTTTTCTGCTGAAATTTCCACTCTCATCGGAAACCTTTGTGCTGAATCAGATTACTGCGTTTATTGA
TATGGGCCATGAGGTGGAGATTGTCGCGTTACAAAAAGGCGATACCCAACATACTCACGCCGCCTGGGAGAAGTATGGCC
TGGCGGCGAAAACCCGCTGGTTACAGGATGAGCCCCAGGGACGGCTGGCGAAACTGCGCTACCGGGCATGTAAAACGCTG
CCGGGGCTGCATCGGGCGGCGACCTGGAAAGCGCTCAATTTTACCCGCTATGGCGATGAATCACGCAATTTGATCCTTTC
CGCGATTTGCGCGCAGGTGAGCCAGCCTTTTGTGGCGGATGTGTTTATCGCACACTTTGGTCCGGCGGGCGTGACGGCGG
CCAAACTACGCGAACTGGGCGTGCTTCGCGGCAAAATCGCGACTATTTTCCACGGGATTGATATCTCTAGTCGTGAGGTG
CTCAGTCATTACACGCCGGAGTATCAGCAGTTGTTTCGTCGTGGCGATCTGATGCTGCCCATCAGCGATCTGTGGGCCGG
TCGCCTGAAAAGTATGGGCTGTCCGCCGGAAAAGATTGCCGTTTCGCGCATGGGCGTCGACATGACGCGTTTTACCCATC
GTTCGGTGAAAGCGCCAGGGATGCCGCTGGAGATGATTTCCGTCGCGCGCCTGACAGAAAAAAAAGGCCTGCATGTGGCG
ATTGAAGCCTGTCGGCAACTGAAAGCACAGGGCGTGGCGTTTCGCTACCGCATTCTGGGGATTGGCCCGTGGGAACGTCG
GCTGCGCACGCTCATCGAGCAGTATCAGCTAGAGGATGTCATTGAGATGCCGGGGTTTAAACCGAGCCATGAAGTGAAGG
CGATGCTGGATGACGCCGATGTTTTTTTGCTGCCGTCGATTACCGGTACGGATGGCGATATGGAAGGTATTCCGGTAGCG
CTGATGGAGGCGATGGCGGTAGGGATTCCCGTGGTATCTACCGTGCATAGCGGTATTCCGGAACTGGTGGAGGCCGGCAA
ATCCGGCTGGCTGGTGCCGGAAAACGATGCGCAGGCGCTGGCGGCCCGACTCGCTGAGTTCAGCCGGATTGACCACGACA
CGCTGGAGTCGGTGATCACGCGCGCCCGTGAAAAAGTGGCGCAAGATTTTAATCAGCAGGCGATTAATCGCCAGTTAGCC
AGCCTGCTACAAACGATATAA

Upstream 100 bases:

>100_bases
TGCCTGCGCTAAAGGCAAAAGTGGCTAACGCCGTCACCGAGGAGCGGGCGAAAGGGTTCACGATGATCAAATCTGTTCTC
GACAGAATTCGGGAGGCGCA

Downstream 100 bases:

>100_bases
ACGAGGTGGTATGCCCGCGACTAAATTCTCCCGACGTACCCTCCTGACGGCAGGTTCTGCGCTTGCTGTTCTTCCTTTTC
TGCGCGCCTTGCCGGTACAG

Product: glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKVSFFLLKFPLSSETFVLNQITAFIDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRWLQDEPQGRLAKLRYRACKTL
PGLHRAATWKALNFTRYGDESRNLILSAICAQVSQPFVADVFIAHFGPAGVTAAKLRELGVLRGKIATIFHGIDISSREV
LSHYTPEYQQLFRRGDLMLPISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFTHRSVKAPGMPLEMISVARLTEKKGLHVA
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDADVFLLPSITGTDGDMEGIPVA
LMEAMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQALAARLAEFSRIDHDTLESVITRAREKVAQDFNQQAINRQLA
SLLQTI

Sequences:

>Translated_406_residues
MKVSFFLLKFPLSSETFVLNQITAFIDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRWLQDEPQGRLAKLRYRACKTL
PGLHRAATWKALNFTRYGDESRNLILSAICAQVSQPFVADVFIAHFGPAGVTAAKLRELGVLRGKIATIFHGIDISSREV
LSHYTPEYQQLFRRGDLMLPISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFTHRSVKAPGMPLEMISVARLTEKKGLHVA
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDADVFLLPSITGTDGDMEGIPVA
LMEAMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQALAARLAEFSRIDHDTLESVITRAREKVAQDFNQQAINRQLA
SLLQTI
>Mature_406_residues
MKVSFFLLKFPLSSETFVLNQITAFIDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRWLQDEPQGRLAKLRYRACKTL
PGLHRAATWKALNFTRYGDESRNLILSAICAQVSQPFVADVFIAHFGPAGVTAAKLRELGVLRGKIATIFHGIDISSREV
LSHYTPEYQQLFRRGDLMLPISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFTHRSVKAPGMPLEMISVARLTEKKGLHVA
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDADVFLLPSITGTDGDMEGIPVA
LMEAMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQALAARLAEFSRIDHDTLESVITRAREKVAQDFNQQAINRQLA
SLLQTI

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family

Homologues:

Organism=Escherichia coli, GI1788357, Length=406, Percent_Identity=85.4679802955665, Blast_Score=706, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17532709, Length=168, Percent_Identity=26.1904761904762, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WCAL_SALTY (P26388)

Other databases:

- EMBL:   X56793
- EMBL:   AE006468
- PIR:   S15296
- RefSeq:   NP_461045.1
- ProteinModelPortal:   P26388
- GeneID:   1253621
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2100
- NMPDR:   fig|99287.1.peg.2025
- HOGENOM:   HBG306876
- OMA:   ICAQVSH
- ProtClustDB:   PRK15427
- BioCyc:   STYP99287:STM2100-MONOMER
- InterPro:   IPR001296

Pfam domain/function: PF00534 Glycos_transf_1

EC number: 2.-.-.- [C]

Molecular weight: Translated: 45194; Mature: 45194

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSFFLLKFPLSSETFVLNQITAFIDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRW
CCEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHCCHHHHHC
LQDEPQGRLAKLRYRACKTLPGLHRAATWKALNFTRYGDESRNLILSAICAQVSQPFVAD
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHEEEHCCCCCHHHHHHHHHHHHHCCCHHHH
VFIAHFGPAGVTAAKLRELGVLRGKIATIFHGIDISSREVLSHYTPEYQQLFRRGDLMLP
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCCEEE
ISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFTHRSVKAPGMPLEMISVARLTEKKGLHVA
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHH
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDAD
HHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
VFLLPSITGTDGDMEGIPVALMEAMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQAL
EEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
AARLAEFSRIDHDTLESVITRAREKVAQDFNQQAINRQLASLLQTI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKVSFFLLKFPLSSETFVLNQITAFIDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRW
CCEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHCCHHHHHC
LQDEPQGRLAKLRYRACKTLPGLHRAATWKALNFTRYGDESRNLILSAICAQVSQPFVAD
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHEEEHCCCCCHHHHHHHHHHHHHCCCHHHH
VFIAHFGPAGVTAAKLRELGVLRGKIATIFHGIDISSREVLSHYTPEYQQLFRRGDLMLP
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCCEEE
ISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFTHRSVKAPGMPLEMISVARLTEKKGLHVA
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHH
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDAD
HHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
VFLLPSITGTDGDMEGIPVALMEAMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQAL
EEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
AARLAEFSRIDHDTLESVITRAREKVAQDFNQQAINRQLASLLQTI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609