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The map label for this gene is cbiA

Identifier: 16765365

GI number: 16765365

Start: 2112422

End: 2113801

Strand: Reverse

Name: cbiA

Synonym: STM2035

Alternate gene names: 16765365

Gene position: 2113801-2112422 (Counterclockwise)

Preceding gene: 16765366

Following gene: 16765364

Centisome position: 43.52

GC content: 55.8

Gene sequence:

>1380_bases
ATGGCGGCAAGGCATCACGCATTTATTCTTGCAGGCACAGGAAGCGGTTGCGGCAAAACCACGGTGACGCTTGGTTTGTT
GAGGCTGCTGCAAAAACGCGCGCTGCGCGTACAGCCGTTTAAAGTTGGGCCAGATTACCTTGATACCGGCTGGCATACCG
CTATCTGTGGCGTGGCGTCCCGTAATCTTGACAGTTTCATGCTTCCTCCTCCTGTTCTCAACGCCTTATTTTGCGAACAA
ATGCGGCAGGCGGATATCGCTGTCATTGAAGGGGTCATGGGGTTGTATGACGGCTATGGCGTCGACCCCAATTACTGCAG
CACAGCCGCGATGGCCAAACAGCTTGGTTGCCCGGTCATCCTACTGGTGGACGGCAAGGCTGTTTCAACGTCGCTTGCGG
CTACCGTTATGGGATTTCAGCACTTCGACCCGACCCTCAACCTCGCGGGCGTGATTGTGAATCGCGTCACTAGCGACGCC
CACTATCAGCTCCTCAAAAATGCCATTGAACATTATTGCTCACTGCCGGTACTGGGGTATGTCCCTCCTTGCGACGGCGT
CGCATTACCTGAACGCCACCTGGGGTTGATTACTGCCAGAGAATCCCTCGTCAATCAGCAATCATGGCATGATTTTGCCG
CCACGCTGGAACAAACAGTGGATGTGGATGCGCTACTCTCGCTGAGCGTGTTATCCGCGCTTCCGGCGGGAATGTGGCCT
GAACGCCCCGATAACACAGCGGGGGCAGGGCTGACGCTTGCTCTGGCGGATGATGAGGCCTTTAACTTTTATTATCCCGA
TAACATCGATTTGCTGGAGCGTGCCGGCGTTAACATTGTGCGTTTTAGCCCACTGCATGACCGCGCATTGCCTGATTGTC
AGATGATCTGGCTGGGCGGTGGATATCCTGAATTATACGCCGCGGATCTGGCGGCAAATACGGTGATGTTAAAACATTTG
CGGGCGGCGCATCAGCGCGGCGTGGCGATTTACGCGGAGTGTGGTGGTCTGATGTATCTGGGGAGCACTCTGGAGGATAG
CGGCGGGGAAATTCATCAGATGGCGAATATTATCCCCGGTCACAGCAAAATGGGTAAGCGACTCACCCGCTTTGGTTATT
GTGAGGCGCAGGCTATGCAGCCAACGCTATTGGCGGCGCCTGGCGAGATAGTGCGCGGCCATGAATTTCACTACTCAGAT
TTTATCCCCGAAACACCCGCCGTTATGGCCTGTCGTAAGGTTCGCGATGGCCGCGTGTTACAGGAATGGACTGGCGGCTG
GCAGACAGGCAACACGTTTGCCAGCTATCTGCACGTCCATTTCGCCCAGCGTCCGGAAATGTTGCAGCACTGGCTGGCGG
CGGCGAGGCGCGTATTATGA

Upstream 100 bases:

>100_bases
AACAACTTCGGTGGGAAGTGGGTTGCGAAGACGCGTACAGTCGAAAGACTGAACATGCGCGTACCTGTATACCCCTACCA
CCCTGAACAGGATCAGGGTA

Downstream 100 bases:

>100_bases
CGATTCTTGCCTGGTGTATCGCCTGGGTGCTGGATTTTATCATCGGCGACCCTCAACACTGGCCCCATCCGGTACGCTGG
ATAGGTCGTTTAATCACGTT

Product: cobyrinic acid a,c-diamide synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MAARHHAFILAGTGSGCGKTTVTLGLLRLLQKRALRVQPFKVGPDYLDTGWHTAICGVASRNLDSFMLPPPVLNALFCEQ
MRQADIAVIEGVMGLYDGYGVDPNYCSTAAMAKQLGCPVILLVDGKAVSTSLAATVMGFQHFDPTLNLAGVIVNRVTSDA
HYQLLKNAIEHYCSLPVLGYVPPCDGVALPERHLGLITARESLVNQQSWHDFAATLEQTVDVDALLSLSVLSALPAGMWP
ERPDNTAGAGLTLALADDEAFNFYYPDNIDLLERAGVNIVRFSPLHDRALPDCQMIWLGGGYPELYAADLAANTVMLKHL
RAAHQRGVAIYAECGGLMYLGSTLEDSGGEIHQMANIIPGHSKMGKRLTRFGYCEAQAMQPTLLAAPGEIVRGHEFHYSD
FIPETPAVMACRKVRDGRVLQEWTGGWQTGNTFASYLHVHFAQRPEMLQHWLAAARRVL

Sequences:

>Translated_459_residues
MAARHHAFILAGTGSGCGKTTVTLGLLRLLQKRALRVQPFKVGPDYLDTGWHTAICGVASRNLDSFMLPPPVLNALFCEQ
MRQADIAVIEGVMGLYDGYGVDPNYCSTAAMAKQLGCPVILLVDGKAVSTSLAATVMGFQHFDPTLNLAGVIVNRVTSDA
HYQLLKNAIEHYCSLPVLGYVPPCDGVALPERHLGLITARESLVNQQSWHDFAATLEQTVDVDALLSLSVLSALPAGMWP
ERPDNTAGAGLTLALADDEAFNFYYPDNIDLLERAGVNIVRFSPLHDRALPDCQMIWLGGGYPELYAADLAANTVMLKHL
RAAHQRGVAIYAECGGLMYLGSTLEDSGGEIHQMANIIPGHSKMGKRLTRFGYCEAQAMQPTLLAAPGEIVRGHEFHYSD
FIPETPAVMACRKVRDGRVLQEWTGGWQTGNTFASYLHVHFAQRPEMLQHWLAAARRVL
>Mature_458_residues
AARHHAFILAGTGSGCGKTTVTLGLLRLLQKRALRVQPFKVGPDYLDTGWHTAICGVASRNLDSFMLPPPVLNALFCEQM
RQADIAVIEGVMGLYDGYGVDPNYCSTAAMAKQLGCPVILLVDGKAVSTSLAATVMGFQHFDPTLNLAGVIVNRVTSDAH
YQLLKNAIEHYCSLPVLGYVPPCDGVALPERHLGLITARESLVNQQSWHDFAATLEQTVDVDALLSLSVLSALPAGMWPE
RPDNTAGAGLTLALADDEAFNFYYPDNIDLLERAGVNIVRFSPLHDRALPDCQMIWLGGGYPELYAADLAANTVMLKHLR
AAHQRGVAIYAECGGLMYLGSTLEDSGGEIHQMANIIPGHSKMGKRLTRFGYCEAQAMQPTLLAAPGEIVRGHEFHYSDF
IPETPAVMACRKVRDGRVLQEWTGGWQTGNTFASYLHVHFAQRPEMLQHWLAAARRVL

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CBIA_SALTY (P29946)

Other databases:

- EMBL:   L12006
- EMBL:   AE006468
- EMBL:   X63012
- PIR:   S20553
- RefSeq:   NP_460980.1
- ProteinModelPortal:   P29946
- PRIDE:   P29946
- GeneID:   1253556
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2035
- NMPDR:   fig|99287.1.peg.1960
- HOGENOM:   HBG634910
- OMA:   VFKCGPD
- ProtClustDB:   PRK01077
- BioCyc:   MetaCyc:MONOMER-13217
- BioCyc:   STYP99287:STM2035-MONOMER
- HAMAP:   MF_00027
- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00379

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: NA

Molecular weight: Translated: 50037; Mature: 49906

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS51274 GATASE_COBBQ; PS00013 PROKAR_LIPOPROTEIN

Important sites: ACT_SITE 334-334 ACT_SITE 438-438

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARHHAFILAGTGSGCGKTTVTLGLLRLLQKRALRVQPFKVGPDYLDTGWHTAICGVAS
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCEECCEECCCCHHCCCHHHHHHHHHH
RNLDSFMLPPPVLNALFCEQMRQADIAVIEGVMGLYDGYGVDPNYCSTAAMAKQLGCPVI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEE
LLVDGKAVSTSLAATVMGFQHFDPTLNLAGVIVNRVTSDAHYQLLKNAIEHYCSLPVLGY
EEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEC
VPPCDGVALPERHLGLITARESLVNQQSWHDFAATLEQTVDVDALLSLSVLSALPAGMWP
CCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCC
ERPDNTAGAGLTLALADDEAFNFYYPDNIDLLERAGVNIVRFSPLHDRALPDCQMIWLGG
CCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHCCCCEEEECCCCCCCCCCCEEEEECC
GYPELYAADLAANTVMLKHLRAAHQRGVAIYAECGGLMYLGSTLEDSGGEIHQMANIIPG
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHCCC
HSKMGKRLTRFGYCEAQAMQPTLLAAPGEIVRGHEFHYSDFIPETPAVMACRKVRDGRVL
HHHHHHHHHHHCCCHHHCCCCEEEECCCHHHCCCCCCHHCCCCCCHHHHHHHHHCCCCHH
QEWTGGWQTGNTFASYLHVHFAQRPEMLQHWLAAARRVL
HHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AARHHAFILAGTGSGCGKTTVTLGLLRLLQKRALRVQPFKVGPDYLDTGWHTAICGVAS
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCEECCEECCCCHHCCCHHHHHHHHHH
RNLDSFMLPPPVLNALFCEQMRQADIAVIEGVMGLYDGYGVDPNYCSTAAMAKQLGCPVI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEE
LLVDGKAVSTSLAATVMGFQHFDPTLNLAGVIVNRVTSDAHYQLLKNAIEHYCSLPVLGY
EEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEC
VPPCDGVALPERHLGLITARESLVNQQSWHDFAATLEQTVDVDALLSLSVLSALPAGMWP
CCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCC
ERPDNTAGAGLTLALADDEAFNFYYPDNIDLLERAGVNIVRFSPLHDRALPDCQMIWLGG
CCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHCCCCEEEECCCCCCCCCCCEEEEECC
GYPELYAADLAANTVMLKHLRAAHQRGVAIYAECGGLMYLGSTLEDSGGEIHQMANIIPG
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHCCC
HSKMGKRLTRFGYCEAQAMQPTLLAAPGEIVRGHEFHYSDFIPETPAVMACRKVRDGRVL
HHHHHHHHHHHCCCHHHCCCCEEEECCCHHHCCCCCCHHCCCCCCHHHHHHHHHCCCCHH
QEWTGGWQTGNTFASYLHVHFAQRPEMLQHWLAAARRVL
HHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609; 1374146