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The map label for this gene is otsB

Identifier: 16765271

GI number: 16765271

Start: 2024696

End: 2025499

Strand: Reverse

Name: otsB

Synonym: STM1929

Alternate gene names: 16765271

Gene position: 2025499-2024696 (Counterclockwise)

Preceding gene: 16765272

Following gene: 16765270

Centisome position: 41.7

GC content: 54.1

Gene sequence:

>804_bases
GTGGCAGAACCGTTAACCGTATCCCCCGAACTCACTGCGAACTATGCGTATTTTTTTGACCTTGATGGCACATTGGCAGA
GATAAAGCCTCATCCCGATCAGGTAGTGGTACCGCATAAAATCCTTCAGTTGTTAGACCGTCTCGCGGCGCACAACGCAG
GGGCACTGGCATTGATTTCAGGGCGTTCAATGACTGAGCTGGATGCTTTGGCGAAGCCTTTTCGCTTTCCACTCGCTGGC
GTTCACGGGGCAGAGCGTCGTGACATCAATGGTAAAACCCATATCGTGCGCCTTCCTGAAGCGGTAGTCCGTGAGGTAGA
GGCGTTATTACGTTCGACGCTTGTAGCGCTGCCCGGAACGGAGCTGGAGTCGAAGGGGATGGCATTTGCGCTGCACTACC
GTCAGGCTCCGGAACATGAAGCGGCGCTGTTGGCGCTTGCGCAACACGTTACGCAGCACTGGCCGCAACTGGCGTTGCAG
CCTGGTAAATGTGTTGTGGAAATCAAACCTAAGGGAACCAATAAAGGCGAAGCCATTGCCGCCTTTATGCAGGAGGCGCC
CTTTGCGGGACGGATTCCCGTTTTTGTGGGCGACGATTTGACCGATGAGGCTGGCTTTGGCGTGGTGAATCACGCTGGCG
GAATTTCCGTAAAAGTGGGCGTCGGGGCAACCCAGGCGGCATGGCGGCTGGAAAGCGTACCCGATGTCTGGCGCTGGCTG
GAACAAATCAATTATCCACAACAAGAACAACAAGTAATGAATAACAGGAGAGATGGCTATGAGTCGTTTAGTCGTAGTAT
CTAA

Upstream 100 bases:

>100_bases
GATGGTCTTTCTAAATGGCGAGCAGCGTCACACTGTCTATACTTACATGACTAATGAGACCGTTTGTGAGTCTCAATATG
ATGATAAGGAGGAGACCAGG

Downstream 100 bases:

>100_bases
TCGAATTGCCCCCCCGGATAATAAAGGCGGCGCCGGCGGCCTCGCCGTTGGCGTGCTTGGCGCGCTAAAAGCGGCTGGCG
GGTTGTGGTTCGGCTGGAGT

Product: trehalose-6-phosphate phosphatase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MAEPLTVSPELTANYAYFFDLDGTLAEIKPHPDQVVVPHKILQLLDRLAAHNAGALALISGRSMTELDALAKPFRFPLAG
VHGAERRDINGKTHIVRLPEAVVREVEALLRSTLVALPGTELESKGMAFALHYRQAPEHEAALLALAQHVTQHWPQLALQ
PGKCVVEIKPKGTNKGEAIAAFMQEAPFAGRIPVFVGDDLTDEAGFGVVNHAGGISVKVGVGATQAAWRLESVPDVWRWL
EQINYPQQEQQVMNNRRDGYESFSRSI

Sequences:

>Translated_267_residues
MAEPLTVSPELTANYAYFFDLDGTLAEIKPHPDQVVVPHKILQLLDRLAAHNAGALALISGRSMTELDALAKPFRFPLAG
VHGAERRDINGKTHIVRLPEAVVREVEALLRSTLVALPGTELESKGMAFALHYRQAPEHEAALLALAQHVTQHWPQLALQ
PGKCVVEIKPKGTNKGEAIAAFMQEAPFAGRIPVFVGDDLTDEAGFGVVNHAGGISVKVGVGATQAAWRLESVPDVWRWL
EQINYPQQEQQVMNNRRDGYESFSRSI
>Mature_266_residues
AEPLTVSPELTANYAYFFDLDGTLAEIKPHPDQVVVPHKILQLLDRLAAHNAGALALISGRSMTELDALAKPFRFPLAGV
HGAERRDINGKTHIVRLPEAVVREVEALLRSTLVALPGTELESKGMAFALHYRQAPEHEAALLALAQHVTQHWPQLALQP
GKCVVEIKPKGTNKGEAIAAFMQEAPFAGRIPVFVGDDLTDEAGFGVVNHAGGISVKVGVGATQAAWRLESVPDVWRWLE
QINYPQQEQQVMNNRRDGYESFSRSI

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family

Homologues:

Organism=Escherichia coli, GI1788207, Length=267, Percent_Identity=73.4082397003745, Blast_Score=407, Evalue=1e-115,
Organism=Drosophila melanogaster, GI19920676, Length=240, Percent_Identity=30, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI221473368, Length=245, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI20129307, Length=245, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24582490, Length=245, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OTSB_SALTY (Q9L894)

Other databases:

- EMBL:   AF213176
- EMBL:   AE006468
- RefSeq:   NP_460886.1
- ProteinModelPortal:   Q9L894
- SMR:   Q9L894
- PRIDE:   Q9L894
- GeneID:   1253450
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM1929
- NMPDR:   fig|99287.1.peg.1868
- HOGENOM:   HBG686849
- OMA:   VGGHGAE
- ProtClustDB:   PRK10187
- BioCyc:   STYP99287:STM1929-MONOMER
- BRENDA:   3.1.3.12
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR01484
- TIGRFAMs:   TIGR00685

Pfam domain/function: PF02358 Trehalose_PPase; SSF56784 SSF56784

EC number: =3.1.3.12

Molecular weight: Translated: 29246; Mature: 29115

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPLTVSPELTANYAYFFDLDGTLAEIKPHPDQVVVPHKILQLLDRLAAHNAGALALIS
CCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCCEECHHHHHHHHHHHHHCCCCEEEEEC
GRSMTELDALAKPFRFPLAGVHGAERRDINGKTHIVRLPEAVVREVEALLRSTLVALPGT
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCC
ELESKGMAFALHYRQAPEHEAALLALAQHVTQHWPQLALQPGKCVVEIKPKGTNKGEAIA
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHH
AFMQEAPFAGRIPVFVGDDLTDEAGFGVVNHAGGISVKVGVGATQAAWRLESVPDVWRWL
HHHHHCCCCCCCCEEECCCCCCCCCCCEEECCCCEEEEECCCCCHHHHHHHCCHHHHHHH
EQINYPQQEQQVMNNRRDGYESFSRSI
HHCCCCHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
AEPLTVSPELTANYAYFFDLDGTLAEIKPHPDQVVVPHKILQLLDRLAAHNAGALALIS
CCCCCCCCCCCCCEEEEEECCCCCEECCCCCCCEECHHHHHHHHHHHHHCCCCEEEEEC
GRSMTELDALAKPFRFPLAGVHGAERRDINGKTHIVRLPEAVVREVEALLRSTLVALPGT
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCC
ELESKGMAFALHYRQAPEHEAALLALAQHVTQHWPQLALQPGKCVVEIKPKGTNKGEAIA
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHH
AFMQEAPFAGRIPVFVGDDLTDEAGFGVVNHAGGISVKVGVGATQAAWRLESVPDVWRWL
HHHHHCCCCCCCCEEECCCCCCCCCCCEEECCCCEEEEECCCCCHHHHHHHCCHHHHHHH
EQINYPQQEQQVMNNRRDGYESFSRSI
HHCCCCHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609