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The map label for this gene is emrE [H]

Identifier: 16764997

GI number: 16764997

Start: 1746615

End: 1746953

Strand: Reverse

Name: emrE [H]

Synonym: STM1653

Alternate gene names: 16764997

Gene position: 1746953-1746615 (Counterclockwise)

Preceding gene: 16764999

Following gene: 16764993

Centisome position: 35.96

GC content: 41.89

Gene sequence:

>339_bases
ATGACTAAAGAAGCTGTAATCTTTCTATTTATCGCCATCGTGGTAGAAGTTATCGCCACGATCTCATTAAAATTATCAGA
TAGTTTTACGCGTCTGGTACCGAGCCTCGTTACTATCATCGGATATTGTATTGCGTTCTGGTGTCTTACCATCCCAATGC
GAACCATCCCTGCGGGTATCATTTATGCCATTTGGTCTGGGGTAGGGATTGTTCTTATTGGATTGATAGGATGGTTGTTT
CTTGGCCAAAAACTGGATGTGCCGGCTATTATTGGCATGTTGCTTATCATCTGCGGCGTAATCGTAATCAATCTGTTTTC
AAAAAGTGTCAGTCACTAG

Upstream 100 bases:

>100_bases
TTATGGCAAGATATGCAATGGTTCAAAAGCGTCTTTATTCTGTCATCCGTATTACTTAAGATATCATCACGACCCGAAAA
ATATTGGAACGAAGAGATGT

Downstream 100 bases:

>100_bases
GCGATGATGATTGATTTGAGCCCGCACTCTCTTATGCGGGCTCGGGTATTTAGCGTACCACCAGTACGGAGCATTCCGCA
TGACGCACAACGGCTGCGGC

Product: membrane transporter of cations

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Efflux-multidrug resistance protein emrE; Ethidium resistance protein; Methyl viologen resistance protein C [H]

Number of amino acids: Translated: 112; Mature: 111

Protein sequence:

>112_residues
MTKEAVIFLFIAIVVEVIATISLKLSDSFTRLVPSLVTIIGYCIAFWCLTIPMRTIPAGIIYAIWSGVGIVLIGLIGWLF
LGQKLDVPAIIGMLLIICGVIVINLFSKSVSH

Sequences:

>Translated_112_residues
MTKEAVIFLFIAIVVEVIATISLKLSDSFTRLVPSLVTIIGYCIAFWCLTIPMRTIPAGIIYAIWSGVGIVLIGLIGWLF
LGQKLDVPAIIGMLLIICGVIVINLFSKSVSH
>Mature_111_residues
TKEAVIFLFIAIVVEVIATISLKLSDSFTRLVPSLVTIIGYCIAFWCLTIPMRTIPAGIIYAIWSGVGIVLIGLIGWLFL
GQKLDVPAIIGMLLIICGVIVINLFSKSVSH

Specific function: Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistanc

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=109, Percent_Identity=57.7981651376147, Blast_Score=121, Evalue=1e-29,
Organism=Escherichia coli, GI1787883, Length=98, Percent_Identity=34.6938775510204, Blast_Score=63, Evalue=5e-12,
Organism=Escherichia coli, GI87082378, Length=102, Percent_Identity=31.3725490196078, Blast_Score=60, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 12148; Mature: 12017

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEAVIFLFIAIVVEVIATISLKLSDSFTRLVPSLVTIIGYCIAFWCLTIPMRTIPAGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYAIWSGVGIVLIGLIGWLFLGQKLDVPAIIGMLLIICGVIVINLFSKSVSH
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKEAVIFLFIAIVVEVIATISLKLSDSFTRLVPSLVTIIGYCIAFWCLTIPMRTIPAGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYAIWSGVGIVLIGLIGWLFLGQKLDVPAIIGMLLIICGVIVINLFSKSVSH
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1936950; 1320256; 9278503; 7896833; 9050242; 9688273 [H]