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The map label for this gene is ydfI [H]

Identifier: 16764853

GI number: 16764853

Start: 1586069

End: 1587535

Strand: Direct

Name: ydfI [H]

Synonym: STM1508

Alternate gene names: 16764853

Gene position: 1586069-1587535 (Clockwise)

Preceding gene: 16764851

Following gene: 16764857

Centisome position: 32.65

GC content: 56.03

Gene sequence:

>1467_bases
ATGCAAAACAGGCTTTTATCGGCAAAGGCGACGCTTCCTGATTACGATCGCGCGGCGCTGGCGGCGCGGATGGTCCATCT
CGGTTTTGGCGCGTTTCATCGCGCGCATCAGGGGGTTTACACAGATATTCTGGCTGCGGAGCAGCATAGCGACTGGGGCT
ATTATGAGGTTAATCTTATTGGCGGCGAACAGCAGATTGCCGATCTAAAGCAGCAGGATAACCTCTATACCGTGGCGGAG
ATGTCGGCGGAAGCCTGGACGGCGCGTGTGGTGGGCGTCGTCAAAGCGGCGTTGCATGTCCAGGTTGATGGTCTGGAACG
TGTGCTGGCGGCGATGTGCGAACCGCAAATAGCGATTGTGTCTCTGACCATTACCGAGAAAGGGTATTGTCATTCGCCAG
CCACGGGCCAACTTCTGCTGGAACATCCCATGATTGCCGCCGACCTGCAAAATCCGCATCAACCGCTAACGGCACCGGGT
ATTATTGTCGAAGCGTTAGCGCGTCGTAAAGCCGCAGGCTTACCGGCATTTACCGTGATGTCCTGCGATAACATGCCGGA
AAATGGGCATGTGACGCGTCAGGTTGTGACGGCTTACGCCCGGGAGGTGGATGCGGAACTGGCGATATGGATCGAGCAAA
ACGTGACATTTCCGTCAACGATGGTTGATCGGATTGTCCCTGCGGTCACGCCGGAGACGCTGGATAAAATCGAACAACTG
ACCGGCGTGCGCGATCCGGCGGGCGTTGCCTGTGAACCGTTTCGTCAGTGGGTGATAGAGGATACGTTTGTGGCGGGTCG
TCCGCTGTGGGAAAACGCGGGCGCGACGTTGGTCGCCGACGTGGTTCCGTTTGAAGAGATGAAACTGCGAATGCTTAACG
GCAGTCATTCATTCCTGGCTTATCTGGGGTATCTTGCCGGCTATCAGCATATTAATGACTGCATGGCTGACGACAACTAT
CGTCTGACCGCGCAGGCGTTAATGTTACGCGAGCAGGCGCCCACGCTGAAAGTCCAGGGGGTAGATTTACAGCGTTATGC
CGATCAGTTGATTGCGCGTTACCGTAATCCGGCGCTGCGTCATCGTACCTGGCAGATTGCGATGGATGGCAGCCAGAAAC
TTCCGCAGCGTATGCTGGACTCCGTTCGCTGGCATCTTGCTAATCATAGCGATTTTGATTTGCTGGCGCTGGGCGTGGCG
GGGTGGATGCGTTACGTTGGCGGCGTGGATGAGCAGGGTAAGGCGATCGACGTCAGCGATCCGCTATTGCCGGTTATTCA
GCGCGCCGTGGCAAACAGTGAAGAAGGCGCCAGTCGTGTTAAGGCGCTGCTGGGAATGGCTGAAATTTTCGGCAACGATT
TGCCGCAGGCGGCGCGGTTTACGCAAAAAGTGCAAGAAGCCTATGACAGTTTACTGACATATGGCGCGAAAGCGAGTGTG
GCGAAATATGCCGAGCGGTTGAAATAG

Upstream 100 bases:

>100_bases
ATGCTGTCCGGTCACTTTTGTTTAATTAGCGTAAATCCAAATGCACCAGGGCAGGCGTTACTCTGCGACTGTTCCGCTTT
CAGAATATAGAGGTTATAAA

Downstream 100 bases:

>100_bases
CGTAACAGAGACCCGGTAGCATTATGCTACCGGGAAGGCCGGAGCGGTTCTTAGCGGCCAGTGGTATCAGTTCATGCCAA
GACGAATCAGTTCGACCGGC

Product: mannitol dehydrogenase

Products: D-fructuronate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MQNRLLSAKATLPDYDRAALAARMVHLGFGAFHRAHQGVYTDILAAEQHSDWGYYEVNLIGGEQQIADLKQQDNLYTVAE
MSAEAWTARVVGVVKAALHVQVDGLERVLAAMCEPQIAIVSLTITEKGYCHSPATGQLLLEHPMIAADLQNPHQPLTAPG
IIVEALARRKAAGLPAFTVMSCDNMPENGHVTRQVVTAYAREVDAELAIWIEQNVTFPSTMVDRIVPAVTPETLDKIEQL
TGVRDPAGVACEPFRQWVIEDTFVAGRPLWENAGATLVADVVPFEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMADDNY
RLTAQALMLREQAPTLKVQGVDLQRYADQLIARYRNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLANHSDFDLLALGVA
GWMRYVGGVDEQGKAIDVSDPLLPVIQRAVANSEEGASRVKALLGMAEIFGNDLPQAARFTQKVQEAYDSLLTYGAKASV
AKYAERLK

Sequences:

>Translated_488_residues
MQNRLLSAKATLPDYDRAALAARMVHLGFGAFHRAHQGVYTDILAAEQHSDWGYYEVNLIGGEQQIADLKQQDNLYTVAE
MSAEAWTARVVGVVKAALHVQVDGLERVLAAMCEPQIAIVSLTITEKGYCHSPATGQLLLEHPMIAADLQNPHQPLTAPG
IIVEALARRKAAGLPAFTVMSCDNMPENGHVTRQVVTAYAREVDAELAIWIEQNVTFPSTMVDRIVPAVTPETLDKIEQL
TGVRDPAGVACEPFRQWVIEDTFVAGRPLWENAGATLVADVVPFEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMADDNY
RLTAQALMLREQAPTLKVQGVDLQRYADQLIARYRNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLANHSDFDLLALGVA
GWMRYVGGVDEQGKAIDVSDPLLPVIQRAVANSEEGASRVKALLGMAEIFGNDLPQAARFTQKVQEAYDSLLTYGAKASV
AKYAERLK
>Mature_488_residues
MQNRLLSAKATLPDYDRAALAARMVHLGFGAFHRAHQGVYTDILAAEQHSDWGYYEVNLIGGEQQIADLKQQDNLYTVAE
MSAEAWTARVVGVVKAALHVQVDGLERVLAAMCEPQIAIVSLTITEKGYCHSPATGQLLLEHPMIAADLQNPHQPLTAPG
IIVEALARRKAAGLPAFTVMSCDNMPENGHVTRQVVTAYAREVDAELAIWIEQNVTFPSTMVDRIVPAVTPETLDKIEQL
TGVRDPAGVACEPFRQWVIEDTFVAGRPLWENAGATLVADVVPFEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMADDNY
RLTAQALMLREQAPTLKVQGVDLQRYADQLIARYRNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLANHSDFDLLALGVA
GWMRYVGGVDEQGKAIDVSDPLLPVIQRAVANSEEGASRVKALLGMAEIFGNDLPQAARFTQKVQEAYDSLLTYGAKASV
AKYAERLK

Specific function: Unknown

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787823, Length=486, Percent_Identity=81.0699588477366, Blast_Score=822, Evalue=0.0,
Organism=Escherichia coli, GI1788497, Length=464, Percent_Identity=56.8965517241379, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI1790779, Length=472, Percent_Identity=59.9576271186441, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI48994885, Length=377, Percent_Identity=24.6684350132626, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790028, Length=268, Percent_Identity=25.7462686567164, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324401, Length=490, Percent_Identity=37.7551020408163, Blast_Score=330, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6320765, Length=490, Percent_Identity=37.7551020408163, Blast_Score=330, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.1.1.57

Molecular weight: Translated: 53897; Mature: 53897

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNRLLSAKATLPDYDRAALAARMVHLGFGAFHRAHQGVYTDILAAEQHSDWGYYEVNLI
CCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEE
GGEQQIADLKQQDNLYTVAEMSAEAWTARVVGVVKAALHVQVDGLERVLAAMCEPQIAIV
CCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHCCCCEEEE
SLTITEKGYCHSPATGQLLLEHPMIAADLQNPHQPLTAPGIIVEALARRKAAGLPAFTVM
EEEEECCCCCCCCCCCCEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
SCDNMPENGHVTRQVVTAYAREVDAELAIWIEQNVTFPSTMVDRIVPAVTPETLDKIEQL
ECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHH
TGVRDPAGVACEPFRQWVIEDTFVAGRPLWENAGATLVADVVPFEEMKLRMLNGSHSFLA
HCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHH
YLGYLAGYQHINDCMADDNYRLTAQALMLREQAPTLKVQGVDLQRYADQLIARYRNPALR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCHHHH
HRTWQIAMDGSQKLPQRMLDSVRWHLANHSDFDLLALGVAGWMRYVGGVDEQGKAIDVSD
CEEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCC
PLLPVIQRAVANSEEGASRVKALLGMAEIFGNDLPQAARFTQKVQEAYDSLLTYGAKASV
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
AKYAERLK
HHHHHHCC
>Mature Secondary Structure
MQNRLLSAKATLPDYDRAALAARMVHLGFGAFHRAHQGVYTDILAAEQHSDWGYYEVNLI
CCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEE
GGEQQIADLKQQDNLYTVAEMSAEAWTARVVGVVKAALHVQVDGLERVLAAMCEPQIAIV
CCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHCCCCEEEE
SLTITEKGYCHSPATGQLLLEHPMIAADLQNPHQPLTAPGIIVEALARRKAAGLPAFTVM
EEEEECCCCCCCCCCCCEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
SCDNMPENGHVTRQVVTAYAREVDAELAIWIEQNVTFPSTMVDRIVPAVTPETLDKIEQL
ECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHH
TGVRDPAGVACEPFRQWVIEDTFVAGRPLWENAGATLVADVVPFEEMKLRMLNGSHSFLA
HCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHH
YLGYLAGYQHINDCMADDNYRLTAQALMLREQAPTLKVQGVDLQRYADQLIARYRNPALR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCHHHH
HRTWQIAMDGSQKLPQRMLDSVRWHLANHSDFDLLALGVAGWMRYVGGVDEQGKAIDVSD
CEEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCC
PLLPVIQRAVANSEEGASRVKALLGMAEIFGNDLPQAARFTQKVQEAYDSLLTYGAKASV
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
AKYAERLK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-mannonate; NAD+

Specific reaction: D-mannonate + NAD+ = D-fructuronate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]