Click here to switch to the map view.

The map label for this gene is nfdA [H]

Identifier: 16764818

GI number: 16764818

Start: 1547817

End: 1549523

Strand: Reverse

Name: nfdA [H]

Synonym: STM1472

Alternate gene names: 16764818

Gene position: 1549523-1547817 (Counterclockwise)

Preceding gene: 16764819

Following gene: 16764817

Centisome position: 31.9

GC content: 56.88

Gene sequence:

>1707_bases
ATGCTAATGATCAGTCTCGTCCCGTCTCTGCTTTCCAGAACCGCGCTTCTCTTTCTGCTCACGGCCACAGGCGCAGCCAC
CGCCGCCCGCCCGGCAGCGGATATTATTTTGCATAACGGTAATATCATCACTCTGAATGATGCCCAGCCGCAGGCCAGCG
CGCTGGCGATTTCCGGCTCGCGGATTGTGGCGATTGGCGATGATACGGCGACAAATGAATGGCGCGGCGACCATACCCGT
ACCATCGATTTACAGGGTAAAACCGTGATACCCGGCCTGACCGATACTCACATCCACGCCATTCGCGGCGGACAAACCTG
GACATTCGAAACCTACTGGTACGACAGCCCTTCGCTTAAAGACGCGCTGGATAAATTACGCGCCGACGCTAACCGTCGTC
CCCACGATCAATGGGTAGCCGTAGTGGGATCGTGGATACCGGCGCAATTTGCAGAAAACCGGGCGCCGACGGTAGCCGAA
CTGAGTCACGCCCTTCCCGATCATCCGGCTTATATTCAGTATCTTTACGACTATGCGTTAGTGAATCAGCGCGGTATAGA
CGTACTTGGCCTTAACAACACCCCTCCTCCTGATTTAGCGGGAATCCGCGTAGAGCGCGACGCAAAAGGTAGCGCCACGG
GGAAATTATTTGGTGACATCGCCGCGTTTAACCAGCTTTTTGCCAGCATAAGTCGTAACGCCGATCGTGAGGGTGGTCTG
CGGCAATTTTTCGCTGATATGAACGCTCGCGGCGTGACCGGCATCATTGACCCCTCTGCCGGGCCTGCCGCCGCTTATGA
GCCTTTATTTGCAATGCGAAACCAGGGGGATTTACCGCTGCGCGTGGGGTATCGCATTCCGGTACAGCCAGAAGCGAAAG
GTCATGAAGCGCAGTGGTTCAGCAACCTGATGGCCTTTCGCCCGGCGCGTGCCGATGACGGGCAACTGGCTTTTCTTGGC
CTGGGGGAAAGCCTGGTGGCCGGAATGAATGACGGCGTGCGGATGGCCCCAGGGTTTTCTTCCTCAGAGCAGGACAAAAC
CGCGCTTCGCCAGGTCGCGACATTTGCGGCAAAACGGGGAATACCGCTTGAGATCCACGCCTATACCGATGATAGCGCCG
ACGCGATATTGACGATTTTTGAGCAGGTAGCTCAGCAGTACGATCTGCGCCCTCTCCGTTGGTCTATTGCGCATCTGAAT
ACCGGTTCGCCACAGACGCTTGAGCGAATGCGTAAGCTGGGTCTGGCATACACTGTGCAAATGGGGCCTTACTTTGAGGG
GCTTGCCATCCGTGACGCCAATCCACCCGGCGCGACGGACAATTCGCCGCCGGTTCGACTGGCGCTGGATAAAGGGCTTG
TCGTAGCTGGCGGTACCGATTCGACGCGTATTGGCATTGCCGGTGTCTGGCACGCTATCGAATATCATATCACCGGTATA
GCGTCAGGCGGTTCCGTGCGTAAACCCGCCAGCGAGCGGCTCACGCGTCTGGAAGCGCTAGCGTTATATACACGTCATGC
CGCCTGGCTCGCCTTTGCCGAACAACACCGGGGCCAGCTTCGCGTCGGAAAACAGGCCGATCTGGCGGTACTCAATCAGC
CATTTATGACGATGCCGGAAGACAGAATTGATACCATTCGCGCTGTTTTGACGCTTGTCGATGGACGCATTGTTCACGAA
AGTCCGGACCTTAACGCCGGACAATGA

Upstream 100 bases:

>100_bases
ATCACCACCGTTCTAAAAACAGAACGCACAGTTCCCTGGGCACAGATTGAACAATTCTTGTTCGCTCTCTATGGTTTGCT
TATCGCCCGACAAAAGGAAC

Downstream 100 bases:

>100_bases
GGTTGTCAGTTGACGCAAAGCCTGCTTTTATAACCCACCAGCGGCGCTGACGGGAAATCTTTTTCGTCAGCGCGCGATAG
AGCTAATGTATTGAGATCCG

Product: periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MLMISLVPSLLSRTALLFLLTATGAATAARPAADIILHNGNIITLNDAQPQASALAISGSRIVAIGDDTATNEWRGDHTR
TIDLQGKTVIPGLTDTHIHAIRGGQTWTFETYWYDSPSLKDALDKLRADANRRPHDQWVAVVGSWIPAQFAENRAPTVAE
LSHALPDHPAYIQYLYDYALVNQRGIDVLGLNNTPPPDLAGIRVERDAKGSATGKLFGDIAAFNQLFASISRNADREGGL
RQFFADMNARGVTGIIDPSAGPAAAYEPLFAMRNQGDLPLRVGYRIPVQPEAKGHEAQWFSNLMAFRPARADDGQLAFLG
LGESLVAGMNDGVRMAPGFSSSEQDKTALRQVATFAAKRGIPLEIHAYTDDSADAILTIFEQVAQQYDLRPLRWSIAHLN
TGSPQTLERMRKLGLAYTVQMGPYFEGLAIRDANPPGATDNSPPVRLALDKGLVVAGGTDSTRIGIAGVWHAIEYHITGI
ASGGSVRKPASERLTRLEALALYTRHAAWLAFAEQHRGQLRVGKQADLAVLNQPFMTMPEDRIDTIRAVLTLVDGRIVHE
SPDLNAGQ

Sequences:

>Translated_568_residues
MLMISLVPSLLSRTALLFLLTATGAATAARPAADIILHNGNIITLNDAQPQASALAISGSRIVAIGDDTATNEWRGDHTR
TIDLQGKTVIPGLTDTHIHAIRGGQTWTFETYWYDSPSLKDALDKLRADANRRPHDQWVAVVGSWIPAQFAENRAPTVAE
LSHALPDHPAYIQYLYDYALVNQRGIDVLGLNNTPPPDLAGIRVERDAKGSATGKLFGDIAAFNQLFASISRNADREGGL
RQFFADMNARGVTGIIDPSAGPAAAYEPLFAMRNQGDLPLRVGYRIPVQPEAKGHEAQWFSNLMAFRPARADDGQLAFLG
LGESLVAGMNDGVRMAPGFSSSEQDKTALRQVATFAAKRGIPLEIHAYTDDSADAILTIFEQVAQQYDLRPLRWSIAHLN
TGSPQTLERMRKLGLAYTVQMGPYFEGLAIRDANPPGATDNSPPVRLALDKGLVVAGGTDSTRIGIAGVWHAIEYHITGI
ASGGSVRKPASERLTRLEALALYTRHAAWLAFAEQHRGQLRVGKQADLAVLNQPFMTMPEDRIDTIRAVLTLVDGRIVHE
SPDLNAGQ
>Mature_568_residues
MLMISLVPSLLSRTALLFLLTATGAATAARPAADIILHNGNIITLNDAQPQASALAISGSRIVAIGDDTATNEWRGDHTR
TIDLQGKTVIPGLTDTHIHAIRGGQTWTFETYWYDSPSLKDALDKLRADANRRPHDQWVAVVGSWIPAQFAENRAPTVAE
LSHALPDHPAYIQYLYDYALVNQRGIDVLGLNNTPPPDLAGIRVERDAKGSATGKLFGDIAAFNQLFASISRNADREGGL
RQFFADMNARGVTGIIDPSAGPAAAYEPLFAMRNQGDLPLRVGYRIPVQPEAKGHEAQWFSNLMAFRPARADDGQLAFLG
LGESLVAGMNDGVRMAPGFSSSEQDKTALRQVATFAAKRGIPLEIHAYTDDSADAILTIFEQVAQQYDLRPLRWSIAHLN
TGSPQTLERMRKLGLAYTVQMGPYFEGLAIRDANPPGATDNSPPVRLALDKGLVVAGGTDSTRIGIAGVWHAIEYHITGI
ASGGSVRKPASERLTRLEALALYTRHAAWLAFAEQHRGQLRVGKQADLAVLNQPFMTMPEDRIDTIRAVLTLVDGRIVHE
SPDLNAGQ

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 61576; Mature: 61576

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMISLVPSLLSRTALLFLLTATGAATAARPAADIILHNGNIITLNDAQPQASALAISGS
CEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEECCC
RIVAIGDDTATNEWRGDHTRTIDLQGKTVIPGLTDTHIHAIRGGQTWTFETYWYDSPSLK
EEEEECCCCCCCCCCCCCEEEEEECCCEECCCCCCCEEEEEECCCEEEEEEEEECCCCHH
DALDKLRADANRRPHDQWVAVVGSWIPAQFAENRAPTVAELSHALPDHPAYIQYLYDYAL
HHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
VNQRGIDVLGLNNTPPPDLAGIRVERDAKGSATGKLFGDIAAFNQLFASISRNADREGGL
HCCCCCEEEECCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCH
RQFFADMNARGVTGIIDPSAGPAAAYEPLFAMRNQGDLPLRVGYRIPVQPEAKGHEAQWF
HHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCEEECEECCCCCCCCCCHHHHH
SNLMAFRPARADDGQLAFLGLGESLVAGMNDGVRMAPGFSSSEQDKTALRQVATFAAKRG
HHHHHHCCCCCCCCCEEEEECCHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCC
IPLEIHAYTDDSADAILTIFEQVAQQYDLRPLRWSIAHLNTGSPQTLERMRKLGLAYTVQ
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEE
MGPYFEGLAIRDANPPGATDNSPPVRLALDKGLVVAGGTDSTRIGIAGVWHAIEYHITGI
ECCCCCCEEEECCCCCCCCCCCCCEEEEECCCEEEECCCCCCEEEHHHHHHEEEEEEEEE
ASGGSVRKPASERLTRLEALALYTRHAAWLAFAEQHRGQLRVGKQADLAVLNQPFMTMPE
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCCCCCCH
DRIDTIRAVLTLVDGRIVHESPDLNAGQ
HHHHHHHHHHHHHCCEEEECCCCCCCCC
>Mature Secondary Structure
MLMISLVPSLLSRTALLFLLTATGAATAARPAADIILHNGNIITLNDAQPQASALAISGS
CEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEECCC
RIVAIGDDTATNEWRGDHTRTIDLQGKTVIPGLTDTHIHAIRGGQTWTFETYWYDSPSLK
EEEEECCCCCCCCCCCCCEEEEEECCCEECCCCCCCEEEEEECCCEEEEEEEEECCCCHH
DALDKLRADANRRPHDQWVAVVGSWIPAQFAENRAPTVAELSHALPDHPAYIQYLYDYAL
HHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
VNQRGIDVLGLNNTPPPDLAGIRVERDAKGSATGKLFGDIAAFNQLFASISRNADREGGL
HCCCCCEEEECCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCH
RQFFADMNARGVTGIIDPSAGPAAAYEPLFAMRNQGDLPLRVGYRIPVQPEAKGHEAQWF
HHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCEEECEECCCCCCCCCCHHHHH
SNLMAFRPARADDGQLAFLGLGESLVAGMNDGVRMAPGFSSSEQDKTALRQVATFAAKRG
HHHHHHCCCCCCCCCEEEEECCHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCC
IPLEIHAYTDDSADAILTIFEQVAQQYDLRPLRWSIAHLNTGSPQTLERMRKLGLAYTVQ
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEE
MGPYFEGLAIRDANPPGATDNSPPVRLALDKGLVVAGGTDSTRIGIAGVWHAIEYHITGI
ECCCCCCEEEECCCCCCCCCCCCCEEEEECCCEEEECCCCCCEEEHHHHHHEEEEEEEEE
ASGGSVRKPASERLTRLEALALYTRHAAWLAFAEQHRGQLRVGKQADLAVLNQPFMTMPE
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCCCCCCH
DRIDTIRAVLTLVDGRIVHESPDLNAGQ
HHHHHHHHHHHHHCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA