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The map label for this gene is leuC [H]

Identifier: 16763501

GI number: 16763501

Start: 129673

End: 131073

Strand: Reverse

Name: leuC [H]

Synonym: STM0111

Alternate gene names: 16763501

Gene position: 131073-129673 (Counterclockwise)

Preceding gene: 16763502

Following gene: 16763500

Centisome position: 2.7

GC content: 58.96

Gene sequence:

>1401_bases
ATGGCCAAAACGTTATACGAAAAATTATTTGATGCCCACGTGGTCTTTGAGGCGCCAAACGAAACGCCGCTGCTGTACAT
CGACCGCCACCTGGTGCATGAAGTCACCTCTCCGCAGGCGTTTGACGGTCTGCGCGCGCACCATCGTCCGGTACGTCAGC
CAGGGAAAACCTTCGCTACGATGGATCACAACGTCTCGACGCAGACTAAAGACATTAATGCTTCCGGTGAAATGGCGCGT
ATCCAGATGCAGGAGCTGATTAAGAACTGTAACGAGTTCGGCGTCGAGCTGTATGACCTGAATCACCCATATCAGGGCAT
CGTCCATGTGATGGGGCCGGAACAGGGCGTCACCCTGCCGGGCATGACCATCGTCTGCGGCGACTCCCACACCGCCACCC
ACGGCGCGTTTGGTGCGCTGGCCTTCGGCATCGGCACTTCTGAGGTAGAACATGTACTGGCGACGCAAACCCTGAAACAG
GGACGCGCTAAAACCATGAAGATTGAAGTCACGGGCAACGCCGCGCCGGGCATTACCGCCAAAGACATCGTGCTGGCGAT
CATCGGTAAAACCGGTAGCGCCGGCGGCACCGGACACGTGGTTGAATTTTGCGGCGACGCTATCCGCGCGCTGAGTATGG
AAGGCCGCATGACGCTGTGCAATATGGCGATTGAGATGGGCGCCAAAGCCGGTCTGGTCGCCCCGGATGAAACCACTTTC
AACTACGTAAAAGGGCGTTTGCACGCGCCGAAGGGCCGCGATTTTGACGAAGCCGTCGAGTACTGGAAAACGCTGAAAAC
CGATGACGGCGCGACCTTTGATACTGTCGTCGCCCTGCGAGCAGAAGAGATCGCGCCGCAGGTGACCTGGGGCACGAATC
CGGGCCAGGTGATTTCCGTCACCGACATCATCCCCGATCCCGCCTCCTTTAGCGATCCGGTTGAGCGCGCCAGCGCCGAA
AAAGCGCTGGCTTATATGGGCTTACAGCCGGGCGTACCGTTAACGGACGTTGCTATCGATAAAGTCTTTATCGGCTCTTG
TACCAATTCACGCATTGAAGATTTGCGCGCGGCGGCGGAAGTCGCCAAAGGGCGCAAAGTTGCGCCGGGCGTGCAGGCGC
TGGTGGTGCCGGGTTCAGGTCCGGTGAAAGCGCAGGCGGAAGCGGAAGGTCTGGACAAGATCTTTATCGAAGCAGGATTT
GAATGGCGCTTACCGGGCTGTTCCATGTGCCTGGCCATGAATAACGACCGCCTGAACCCGGGCGAGCGCTGCGCCTCCAC
CAGCAACCGTAACTTTGAAGGTCGTCAGGGCCGCGGGGGTCGCACGCATTTAGTCAGCCCGGCGATGGCCGCCGCTGCCG
CCGTTACCGGCCACTTCGCCGACATTCGCAGCATCAAATAA

Upstream 100 bases:

>100_bases
AGGGCGTTCGCACCGGCGATTTAGCCAGGGGCGCTGCCGCAGTCAGTACCGATGAGATGGGCGATATTATCGCCCGCTAT
GTCGCAGAAGGGGTGTAATC

Downstream 100 bases:

>100_bases
GGAAACTACCATGGCAGAGAAATTTACCCAGCATACCGGCCTGGTTGTCCCACTGGATGCCGCCAACGTCGATACCGATG
CAATTATCCCTAAACAGTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAKTLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHHRPVRQPGKTFATMDHNVSTQTKDINASGEMAR
IQMQELIKNCNEFGVELYDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
GRAKTMKIEVTGNAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTF
NYVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVALRAEEIAPQVTWGTNPGQVISVTDIIPDPASFSDPVERASAE
KALAYMGLQPGVPLTDVAIDKVFIGSCTNSRIEDLRAAAEVAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGF
EWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRSIK

Sequences:

>Translated_466_residues
MAKTLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHHRPVRQPGKTFATMDHNVSTQTKDINASGEMAR
IQMQELIKNCNEFGVELYDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
GRAKTMKIEVTGNAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTF
NYVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVALRAEEIAPQVTWGTNPGQVISVTDIIPDPASFSDPVERASAE
KALAYMGLQPGVPLTDVAIDKVFIGSCTNSRIEDLRAAAEVAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGF
EWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRSIK
>Mature_465_residues
AKTLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHHRPVRQPGKTFATMDHNVSTQTKDINASGEMARI
QMQELIKNCNEFGVELYDLNHPYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQG
RAKTMKIEVTGNAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTFN
YVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVALRAEEIAPQVTWGTNPGQVISVTDIIPDPASFSDPVERASAEK
ALAYMGLQPGVPLTDVAIDKVFIGSCTNSRIEDLRAAAEVAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFE
WRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRSIK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=410, Percent_Identity=26.5853658536585, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI8659555, Length=402, Percent_Identity=27.1144278606965, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI41352693, Length=385, Percent_Identity=27.012987012987, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=94.6351931330472, Blast_Score=905, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=364, Percent_Identity=28.8461538461538, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081781, Length=354, Percent_Identity=26.5536723163842, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI2367097, Length=364, Percent_Identity=25.8241758241758, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=412, Percent_Identity=28.6407766990291, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=412, Percent_Identity=28.6407766990291, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=307, Percent_Identity=30.6188925081433, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=457, Percent_Identity=27.7899343544858, Blast_Score=108, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=60.0849256900212, Blast_Score=579, Evalue=1e-166,
Organism=Saccharomyces cerevisiae, GI6320440, Length=417, Percent_Identity=26.6187050359712, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6323335, Length=367, Percent_Identity=29.4277929155313, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6322261, Length=402, Percent_Identity=26.6169154228856, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI281365315, Length=410, Percent_Identity=24.8780487804878, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17864292, Length=410, Percent_Identity=24.8780487804878, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161076999, Length=410, Percent_Identity=24.8780487804878, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28571643, Length=409, Percent_Identity=25.4278728606357, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645686, Length=369, Percent_Identity=27.10027100271, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI17137564, Length=454, Percent_Identity=25.3303964757709, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 49786; Mature: 49655

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHHRPVRQPGKTFAT
CCHHHHHHHCCCEEEEECCCCCCEEEECHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEE
MDHNVSTQTKDINASGEMARIQMQELIKNCNEFGVELYDLNHPYQGIVHVMGPEQGVTLP
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVTGNAAPGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
KDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTF
HCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHH
NYVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVALRAEEIAPQVTWGTNPGQVISV
HHHHCEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEE
TDIIPDPASFSDPVERASAEKALAYMGLQPGVPLTDVAIDKVFIGSCTNSRIEDLRAAAE
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEECCCCCHHHHHHHHHHH
VAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNP
HHCCCCCCCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCCH
GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRSIK
HHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKTLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHHRPVRQPGKTFAT
CHHHHHHHCCCEEEEECCCCCCEEEECHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEE
MDHNVSTQTKDINASGEMARIQMQELIKNCNEFGVELYDLNHPYQGIVHVMGPEQGVTLP
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVTGNAAPGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
KDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTF
HCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHH
NYVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVALRAEEIAPQVTWGTNPGQVISV
HHHHCEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEE
TDIIPDPASFSDPVERASAEKALAYMGLQPGVPLTDVAIDKVFIGSCTNSRIEDLRAAAE
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEECCCCCHHHHHHHHHHH
VAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNP
HHCCCCCCCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCCH
GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRSIK
HHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA